동물들은 항상성 유지를 위해서 지방에 축적된 에너지의 양을 판단하고 섭식량을 조절한다고 알려져 있으나 그 조절 메카니즘은 아직 확실히 알려져있지 않다. 본 연구에서는 노랑초파리로 지방세포에서 섭식조절과 관련된 유전자를 찾기 위해 mRNA의 과발현을 이용한 1차 스크리닝(screening)과 RNAi를 이용한 2차 스크리닝을 수행하여 지방세포에서 발현하는 유전자 purple이 섭식조절과 관련이 있다는 것을 확인하였다. 유전자 purple은 조효소인 tetrahydrobiopterin (BH4)의 생합성에 관여하는 유전자인데, 연구결과 BH4생합성에 관련된 다른 유전자인 Punch와 Sepiapterin Reductase (Sptr) 뿐만 아니라 BH4도 섭식행동을 조절하는데에 관여한다는 것을 밝혀냈다. 흥미로운 사실은 Punch와 purple은 지방에서 발현되어야 하는 반면 Sptr은 뇌에서 발현되어 섭식행동을 조절한다는 사실이다. 추가연구를 통해 BH4가 섭식에 관련된 뉴런인 neuropeptide F (NPF)을 억제하여 섭식억제가 이루어지는 사실을 확인하였다. 본 연구는 새로운 유형의 섭식억제제 개발에 기초적인 정보를 제공할 것으로 보인다.
The family Nabidae (Hemiptera: Heteroptera: Cimicomorpha) is a cosmopolitan family of medium-sized (4-12 mm.) predatory groups in true bugs. Among them, 10 genera and approximately 130 species are distributed in the Palaearctic region. Most Nabidae are predators on various stages of insects (e.g., aphids, leafhoppers and lepidopteran eggs and young caterpillars), but several genera within the subfamily Prostemmatinae (e.g., Prostemma, Alloeorhynchus and Pagasa) are specialized predators of true bugs, especially of the family Lygaeidae. Most Nabinae are herbicolous and arboricolous, whereas all Prostemmatinae are ground-inhabiting (in litter layer, under stones, etc.). In this talk the family Nabidae is reviewed from Korea including two unrecorded species and one new species. The morphological information such as description and diagnoses, and biological information for each species are provided.
Pictorial key to pupa of the genus Olenecamptus in Korea is provided. This study was carried out with pupa of five species in Korea: Olenecamptus formosanus Pic, 1914, Olenecamptus octopustulatus (Motschulsky, 1860), Olenecamptus clarus Pascoe, 1859, Olenecamptus cretaceus cretaceus Bates, 1873 and Olenecamptus subobliteratus Pic, 1923. The description of life cycle and host-plant of the genus Olenecamptus are also given.
분류학은 18세기 린네에 의해 체계화되고 유럽 각국의 전 세계 탐험과 이를 통해 확보한 생물표본을 바탕으로 자연스럽게 발달하여 현재에 이르고 있다. 유럽의 수많은 자연사박물관, 대학연구기관 등은 표본의 수집, 보존, 전시, 교육, 연구에 관한 수백 년간의 노하우가 축적되었으며 문화 속에 자연스럽게 스며들었으나, 국내에는 잘 알려지지 않았다. 이에 필자는 지난 5년간의 폴란드 곤충학 박사과정 동안 유럽 전역의 분류학 연구기관들을 방문하고 이들과 함께 생활하면서 익힌 그들의 분류 문화와 주요 연구기관들을 국내에 소개하고자 한다. 특히 유럽의 연구시스템, 표본 보관방식, 유럽학자들의 특징 등을 중점적으로 알려 앞으로의 국내 분류학 연구에 도움이 되고자 한다.
Pheromone biosynthesis activating neuropeptide (PBAN) produced in the suboesophageal ganglion stimulates pheromone biosynthesis in the pheromone gland, mediating sexual behaviors. Based on the transcriptome of the head, PBAN in the legume pod borer, Maruca vitrata, was identified. To examine the pheromonotropic activity of PBAN in the legume pod borer, Maruca vitrata, a PBAN (Mvi-PBAN) was synthesized. When female adults were injected with a synthetic Mvi-PBAN, pheromone production showed a maximal increase 2 h post-injection. PBAN was expressed in all examined tissues and developmental stages. In contrast, PBAN receptor (PBANr) was detected in the female tissues and all developmental stages except for adult male. In addition, two types of PBANr were identified from the transcriptome of the pheromone gland, suggesting that the molecular signal on the pheromone gland may transduce via PBANr.
Tingidae (Hemiptera: Heteroptera), commonly called “Lace Bugs”, is reported with more than 300 genera and 2,500 species and widely distributed in the world. All of the members of Tingidae are phytophagous, and have associations with their host plants. Some species are known as serious insect pests in agriculture and in forestry (including landscaping trees) in many countries. In this talk, Physatocheila sp. nov. is described from the Korean Peninsula. The morphological information of the Korean congeners and a key to the Korean Physatocheila species are presented with photographs and illustrations.
The superfamily Psylloidea of Korean Peninsula is systematically revised. Systematic studies on the Psylloidea are conducted mainly three themes: (1) The taxonomic review of superfamily Psylloidea (Hemiptera: Sternorrhyncha) in the Korean Peninsula, (2) DNA barcoding for the correction of taxonomic confusions and confirmation of seasonal forms, and (3) molecular phylogeny of Psylloidea to elucidate higher group relationships and to test current controversial morphological classifications.
Calaphidinae is one of the most species rich group in Aphididae. However their phylogenetic relationship is poorly understood. In this study, we reconstructed phylogenetic relationship of Calaphidinae and closely related groups to confirm the taxon boundary and ingroup relationship such as tribe and genera. In addition, evolutionary hypothesis of ancestral host-plant relationship was tested. We performed Bayesian inference and maximum likelihood analyses of combined 4 mitochondrial (ATP6, COI, COII, CytB) and 1 nuclear (EF1α) sequences. Phylogenetic results suggested that Calaphidinae is not a monophyletic and Saltusaphidinae is nested in Calaphidinae. Phyllaphidinae forms a basal clade as the most primitive group of Calaphidinae. Reconstruction of ancestral host plant of Calaphidinae indicated that Calaphidinae might have evolved on members of Fagales plants.
최근 여름부터 가을까지 전국적으로 말벌의 활동이 급증하면서 벌에 쏘이는 사고가 끊이지 않는 등 매우 심각한 사회적 문제가 되고 있다. 그러나 말벌 공격에 대한 잘못된 대응방식이 만연되어져 있어 그 피해를 더 가중시키는 상황이다. 따라서 본 연구에서는 색깔에 따른 말벌의 방어행동에 대한 특성을 분석하였다. 외래종 등검은말벌의 벌집 앞에 8가지 색상의 털뭉치를 설치하여 벌집에 충격을 주었을 때 검은색에 평균 7.6마리(66%)가 공격하여 가장 높게 나타났고 갈색이 3.3마리(28%)로 다음을 이었다. 그 외 노랑, 초록, 주황, 회색, 빨강, 흰색 등에는 공격빈도가 거의 없거나 매우 낮게 나타났다. 이는 각 개별 색깔에 대한 실험에서도 유사한 결과를 나타내었고 농도별(검정, 회색, 흰색) 실험에서도 짙은 농도일수록 더 많은 공격을 받았다. 이는 잠재적인 말벌 천적들의 색깔에 대한 자연선택적 방어 행동일 것으로 판단된다.
The leafhopper subfamily, Typhlocybinae (Hemiptera: Auchenorrhyncha: Cicadellidae), caused direct damage by sap-sucking or indirect damage by transmission of viruses or diseases to the host plants, so Typhlocybinae is one of the most important insect pests in agriculture, urban ecology, forest, etc. in the world. However, their identifications are very difficult due to small size and morphological similarities, and their exact biological information such as host plants and life cycle are not reported yet for most species. So, we construct an integrated biological database that presents accurate diagnostic methods (illustrated key characters, DNA barcoding data), patterns and seasons of occurrences, and some ecological photographs such as dorsal habitus of specimens and plant damages
False click beetles have been barely studied in the Korean peninsula. As a result of the present study, a total of 22 species belonging to 14 genera of Eucnemidae are reviewed from Korea, including two new species and 15 new records. Also, we conducted molecular phylogenetic study of the family Eucnemidae for estimating monophyly based on molecular fragments of mitochondrial protein coding gene (cytochrome c oxidase subunit I gene, COI) and two nuclear ribosomal RNA genes (18S rRNA and 28S rRNA) by Bayesian Inference (BI) and Maximum Likelihood (ML) analysis. The result of the phylogenetic analyses indicated that Eucnemidae is a monophyletic group in Elateroidea, however, mostly form paraphyly compared to previous classification in subfamily level.
Insect chitinases (CHTs), an extracellular enzyme, belong to family 18 glycosyl hydrolases that hydrolyze chitin by an endo-type manner. In insect genomes, there are a large number of genes encoding CHT-like proteins, and they have been classified into eleven groups based on phylogenetic analysis. In this study, we have investigated functions of a group III chitinase (TcCHT7) in Tribolium castaneum. Although, unlike most insect CHTs, TcCHT7 contains a predicted transmembrane segment in N-terminal, immunohistochemical analysis reveals that it is localized in the newly forming procuticle, suggesting that TcCHT7 is released from the plasma membrane of underlying epidermal cells. RNAi for TcCHT7 does not affect on any types of molting. However the resulting pupae and adults fail to undergo wing-expansion and abdominal contraction. In addition, TcCHT7-deficient insects exhibit ultrastructural defects in both rigid (e.g. elytron) and soft (e.g. hindwing) cuticles. These results demonstrate that functional importance of TcCHT7 in the formation of the rigid and soft cuticles of the beetle.
Miridae, commonly plant bugs, is the most diverse group in Heteroptera, with more than 11,000 species, and is widely distributed in the world. This group has a great attention by taxonomists, applied entomologists, and evolutionary biologists because of their great morphological diversity, high biodiversity as well as association with host plants. However, there have been a few phylogenetic studies within this family to date. In this talk, we present the first phylogenetic analyses of the largest tribe Mirini in the family Miridae based on nuclear (18S rRNA and 28S rRNA) and mitochondrial (COI and 16S rRNA) sequence data, based on the results, we discuss on the phylogenetic relationships and classifications within the tribe.
Despite the importance of behavioral ecotoxicological tests, few researches have been conducted to assess the quality of freshwater using behavior of test species, in part due to lack of standardized test method for quantifying the response. In this study, we developed a new test methods using non-biting midge larva’s burrowing behavior to assess field water quality and a new index, AUC (area under curve), to compare burrowing behavior quantitatively. Four kinds of field samples (residential, natural, agricultural and industrial areas) were tested using the method to validate our methodology and index. There were not significant differences in 96 h mortality and heavy metal concentration between samples, but AUC from the industry sample was significantly decreased.
Insect infestation leads to huge loss of agricultural products and transmission of vector-borne diseases causing millions of deaths per annum. Juvenile hormone (JH) controls the development and reproduction of insects, therefore the grouth of insects can be inhibited by interfering the action of JH. Based on this, we developed a novel insect species-specific screening system to identify juvenile hormone antagonists (JHANs) from natural origin. These compounds can disrupt JH-mediated insect development by interfering the binding of a heterodimer, steroid receptor coactivator (SRC), with its partner protein, the methoprene-tolerant (Met) JH receptor. This screening system could be used as a new tool to develop eco-friendly and species-specific insecticides.
An entomopathogenic fungus, Beauveria bassiana ARP14, was isolated from field collected Riptortus pedestris (F.). Lethal concentration of ARP14 was compared with commercialized GHA strain against R. pedestris and its egg parasitoids Ooencyrtus nezarae Ishii and Gryon japonicum (Ashmead). Mortality and infection rate were evaluated in five concentrations, i.e., 1×10 9 , 1×10 8 , 1×10 7 , 1×10 6 , and 1×10 5 conidia/mL using glass scintillation vial. The LC50 of 2nd, 4th instar, and adult stage of R. pedestris was not different between the two strains. The infection rate of ARP14 was higher than that of GHA in 4th instar and adult stage in 1×10 8 conidia/mL. The infection rate was low in ARP14 than GHA in both egg parasitoids. Therefore, our results suggest that the ARP14 strain can be used against R. pedestris with less negative effect on egg parasitoids than GHA strain.
Many turfgrass pests, Ectinohoplia rufipes, Exomala orientalis and Popillia quadriguttata, cause serious damage to grass fields in Korea. In this study, control of turfgrass pests using entomopathogenic fungi were investigated using Protaetia brevitarsis seulensis as an alternative insect. In a preliminary experiment, some Metathizium sp. showed ca. 60% virulence after 7 days exposure to the entomopathogenic fungi. Compared to the contact-exposure, overall a spray treatment showed lower control efficacy against P. brevitarsis seulensis. Furthermore, mortality of various developmental stages of P. brevitarsis seulensis was investigated under laboratory conditions. This work suggests that when treated with fungi mortality of P. brevitarsis seulensis was closely related with developmental stage. These results provide a basic information for the control of P. brevitarsis seulensis using entomopathogenic fungi
현재 적산온도는 절지동물의 생물학적 현상(우화, 생식, 성장 등)의 발생시기를 추정하는데 매우 유용하게 활용되고 있으며 이에 따라 지금까지 많은 적산온도 계산법이 개발되어왔다. 하지만 우리나라에서 활용되고 있는 적산온도 모형의 계산방법은 대부분 mean-minus법을 기반으로 하고 있다. 본 연구에서는 국내 기상환경에서 적산온도를 계산하는 방법에 따라서 그 결과가 어떻게 나타나는지 국내에 분포하고 있는 여러 곤충(솔수염하늘소, 진딧물, 가루깍지벌레 등)들을 대상으로 그 차이를 평가하였다. 그 결과, 기존에 주로 활용되고 있는 mean-minus법을 기반으로 계산할 경우 국내 기상환경에서 특정한 시기에 많은 오차가 발생하는 것으로 나타났으며, 이러한 문제를 해결하기 위해서 다른 계산방법(sine wave method 등)이 대안으로서 활용 될 수 있는 것으로 평가되었다
Asitic apple leafminer, Phyllonorycter ringoniella Matsumura, is an important pest in apple orchard, however its reproductive characteristics and seasonal adaption is poorly understood. The longevity and fecundity of P. ringoniella were investigated at seven constant temperatures (13.7, 15.6, 20.5, 23.7, 26.1, 30.2, and 32.3℃), 60-80% RH, and a photoperiod of 14:10 (L:D) h. Female adult longevity increased as the temperature decreased. The highest fecundity was obtained at 15.6℃ (65.2 eggs/female), and the fecundity decreased as temperature increased. The oviposition model were comprised by four components: adult aging rate model, total fecundity model, age-specific oviposition rate model, and age-specific survival rate model. Female adult aging rates were well described by an inverse second-order polynomial function and the total fecundity model was well described by an extreme value function. Age-specific cumulative oviposition rate was well fitted to a three-parameter Weibull function and age-specific survival rate to a sigmoid function, respectively. The model would be useful in developing population model for P. ringoniella and establishing management strategy against P. ringoniella in apple orchards.