간행물
한국응용곤충학회 학술대회논문집
- 발행기관 한국응용곤충학회
- 자료유형 학술대회
- 간기 반년간
- 수록기간 2000 ~ 2024
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 495DDC 595
권호리스트/논문검색
2010 한국응용곤충학회 심포지엄 및 추계학술발표회 (2010년 10월) 172건
161.
2010.10
구독 인증기관·개인회원 무료
해충에 대한 화학농약의 오남용으로 인하여 해충의 저항성이 발달되고 환경오염, 인축에 대한 위험 등의 이유로 화학농약의 사용을 줄이게 되었다. 그래서 친환경적인 방제인자인 미생물농약 Bacillus thuringiensis(이하 B.t)가 현재 전 세계적으로 널리 사용되고 있다. B.t는 그람 양성의 호기성 간균으로 토양, 곡물의 분진, 낙엽, 곤충의 사체 등 다양한 곳에서 분리되는 것으로 알려져 있는데, 본 연구에서는 풍뎅이류 곤충의 사체로부터 분리한 B.t subsp. kurstaki CAB530 균주가 나비목 해충에 대해서 살충활성을 나타내는 것을 확인하였으며, 특히 난방제 해충인 파밤나방에 살충활성이 뛰어난 KB099 균주와 비교하기 위하여 SDS-PAGE와 PCR을 수행하였다. 파밤나방에 대한 생물활성 검정 결과, CAB530 균주는 처리 48시간 후에 90%가 넘는 사충률을 보여주었며, 다른 B.t subsp. kurstaki SDS-PAGE 수행 결과 파밤나방에 살충활성이 있는 B.t subsp. kurstaki와 비슷한 130kDa의 밴드를 나타내었다. 또한 파밤나방 중장액으로 소화를 시킨 후에 약 65kDa의 활성독소를 확인할 수 있었다. 파밤나방에 6%의 사충율을 보이며 낮은 살충활성을 보인 CAB533 균주는 65kDa의 독소단백질 밴드를 나타냈지만 파밤나방에 대해서 살충활성이 높지는 않았다. PCR수행 결과 파밤나방에 대해서 가장 활성이 높은 CAB530 균주는 Cry1Aa, 1Ab, 1Ac, 1B, 1D, 1E, 1F, 1I, Cry2 유전자가 존재하는 것으로 밝혀졌다.
162.
2010.10
구독 인증기관·개인회원 무료
Mi Ri Sohn, Kwang Sik Lee, Bo Yeon Kim, Young Moo Choo, Soo Dong Woo, Sung Sik Yoo, Yeon Ho Je, Jae Young Choi, Jong Yul Roh, Hyun Na Koo, Young In Kim, Byung Rae Jin
Classical swine fever virus (CSFV) envelope glycoprotein E2 is the main target for inducing neutralizing antibodies and protective immunity in swine. Here, we report a novel strategy forthe large-scale production of a CSFV E2 subunit vaccine that demonstrates a high immunogenic capability in the larvae of a baculovirus-infected silkworm, Bombyx mori. We constructed a recombinant B. mori nucleopolyhedrovirus (BmNPV) that expressed recombinant polyhedra together with the N-terminal 179 amino acids of CSFV E2 (CSFV E2ΔC). BmNPV-E2ΔC-infected silkworm larvae expressed an approximately 44-kDa fusion protein that was detected using both anti-polyhedrin and anti-CSFV E2 antibodies. Electron and confocal microscopy both demonstrated that the recombinant polyhedra were morphologically normal and contained CSFV E2ΔC. The CSFV E2ΔC antigen produced in BmNPV-E2ΔC-infected silkworm larvae reached 0.68 mg per ml of hemolymph and 0.53 mg per larva at 6 days post-infection. Mice that were immunized with the granule form of recombinant polyhedra or the soluble form of the fusion protein elicited CSFV E2 antibodies, which indicated that the recombinant polyhedra carrying CSFV E2ΔC were immunogenic. The virus neutralization test showed that the serum from mice that were treated with recombinant polyhedra or the soluble form of the fusion protein contained significant levels of virus neutralization activity. These results demonstrate that the present strategy can be used for the large-scale production of CSFV E2 antigen and that the recombinant polyhedra containing CSFV E2ΔC as a granule antigen can be used as a potential subunit vaccine against CSFV.
163.
2010.10
구독 인증기관·개인회원 무료
This study presented a development of a phototactic chamber used for pest monitoring. The chamber was constructed by opaque acrylic body. Transparent acrylic wall of the chamber for light-exposure were fitted at both side end parts of the inside chamber. Side parts of the outside chamber were made of removable cover in combination with the air circulation system and light source such as LED or fluorescent. The insect entrance holes was positioned at the center part of the chamber to efficiently dispersed pests, and then nylon net was equipped inside the chamber to prevent the escape of inserted pests. Two opaque partition walls of the inside chamber were made of the movable plate, in order to the control of the light-exposure and the response termination. We also carried out behavioral experiment against various pest species by using the phototactic chamber. Consequently, the phototactic chamber was confirmed suitable result of behavioral experiment. Therefore, we believed that the test chamber help to understand the phototactic responses of various pests.
164.
2010.10
구독 인증기관·개인회원 무료
Insects or insect remains found in beer are one of major issues in consumer claim. Accurate estimation of inflow time isa critical factor for the settlement of such claims related with beer-contaminating insects but no reliable methods have been developed. In an attempt to establish a molecular marker-based diagnostic method, the degradation rates of 18S rRNA genes in the insectssoaked in 500 ml beer were investigated by quantitative real-time PCR (qPCR) over one month period at room temperature. Among the six insect species tested, the house fly (Musca domestica) and honey bee (Apis mellifera) revealed high correlations (r2=0.974-0.990) between the degradation of 18S rRNA gene and inflow time. In these insects, statistically significant distinction was possible between the samples stored in beer less than 14 days and more than 14 days. Other insects, including the fruit fly, common house mosquito, German cockroach and Indian meal moth, displayed poor correlations, which appeared attributed to the inefficient genomic DNA extraction likely due to small sample size or disintegration of body parts during storage in beer. With proper improvement in DNA extraction, this 18S rRNA-based diagnostic method would be applicable for estimating the inflow time of beer-contaminating insects.
165.
2010.10
구독 인증기관·개인회원 무료
Yong Wang, Jae Young Choi, Jong Yul Roh, Qin Liu, Xueying Tao, Jong Bin Park, Soo Dong Woo, Byung Rae Jin, Yeon Ho Je
We isolated two baculoviruses, Spodoptera litura granulovirus (SlGV) and S. litura nucleopolyhedrovirus (SlNPV) in the dead larvae of S. litura. The granule of SlGV were ovoidal shape with an approximate measure of 240-340 nm×140-180 nm, and each granule contained one single rod-shape virion with a mean size of 180-200 nm×20-40 nm. Whereas, the polyhedra of SlNPV were irregular in shape with a approximate diameter of 1.0-1.5 ㎛, and numerous virions comprised of the multinucleocapsid were contained in each polyhedra. The major component of occlusion bodies produced by SlGV and SlNPV were about 29 and 30 kDa, respectively. When the phylogenic relationship between these viruses were analyzed using the nucleotide sequences of granulin gene from SlGV and polyhedrin gene from SlNPV, they were not closely related to each other. We also found that the two viruses showed similar insecticidal activity against 2nd instar larvae of Spodotera litura in terms of dose-response, but SlGV showed much longer LT50 than that of SlNPV. The two baculoviruses might be cooperatively be applied as biological control agent for the control of S. litura
166.
2010.10
구독 인증기관·개인회원 무료
Scutellonema. brevistyletum was collected at soil around the roots of oriental melon (Cucumis melo L. var. makuwa Makino) at Seongju, Gyeongbuk in Korea. Scutellonema brevistyletum resembles to S. sorghi in having large number of longitudinal lines (16-18 vs 16 in S. sorghi), but differs by the areolated at level of scutellum(not areolated in S. sorghi), shorter tail length(9.1-13.3㎛ vs 16.2-35.7㎛ in S. soghi) and not long intestinal overlap rectum. S. brevistyletum differs from S. unum in having shorter stylet (24.5-28㎛ vs 29-32㎛ in S. unum) and basal lip not having longitudinal striae.
167.
2010.10
구독 인증기관·개인회원 무료
This study is based on specimens which had been collected from Jeollabuk-do, Chungcheonbuk-do and Gyeonsangnam-do provinces in July 1994 through September 2009. As a result of this study, Cunaxa potchensis and C. mageei were identified and newly recorded from Korea.
168.
2010.10
구독 인증기관·개인회원 무료
evolution of multicellular organisms. Especially in insects, a large amount of bacterial symbionts are illustrated. Among the insect taxonomic group, sap feeding insects, mostly Heteroptera, that feeds on poor nutrient sources from plants, have developed special organs where symbiotic bacteria can reside. The bacteria mainly provide the host insects with some essential amino acids. This has built evolutionarily unbreakable tie between the symbionts and the their hosts. Here we present an obligate symbiont from Lycorma delicatula (Heteroptera: Fulgoridae) being thought to invade the Korean penninsula recently. Based on the partial 16s rRNA gene sequence, the symbiotic bacterium is identified as Cadidatus Sulcia mullleri. The Genbank data indicates that the bacterium is found in various heteropteran families. This may imply the acquisition of the bacterium precedes the hosts' divergence, though there is an alternative postulation. We discuss its distribution in the sap-feeding insects and potential role on survival of L. delicatula and paratransgenetic application of the bacterium in controlling L. delicatula.
169.
2010.10
구독 인증기관·개인회원 무료
본 연구는 충청남도 천안시 병천면과 공주시 정안면에서 재배되는 오이를 대상으로 반촉성 재배시기인 2월부터 6월까지 수행하였다. 반촉성 재배시기에 문제시 되는 해충은 목화진딧물, 꽃노랑총채벌레, 싸리수염진딧물, 차먼지응애 순이었으며 복숭아혹진딧물, 차응애, 온실가루이, 담배가루이, 긴털가루응애, 선충류 등 11종이 주요해충으로 발생하였다. 목화진딧물과 꽃노랑총채벌레는 살충제를 이용한 포장에서 문제가 심각하였으며 특히 목화진딧물의 경우 농약방제보다 무농약과 저농약 방제에서 밀도가 낮았다. 싸리수염진딧물은 무농약 재배포장에서 일부 발생되었으며 차먼지응애는 제초를 위해 부숙되지 않은 낙엽을 활용한 농가에서 대발생 하였다. 온실가루이와 담배가루이의 발생은 미비하였다. 목화진딧물 방제를 위해 콜레마니진디벌과 천적유지식물을 이용한 결과, 재배가 끝나는 시기인 6월 말까지 피해가 없었으며 목화진딧물 발생전 천적유지식물의 투입과 발생초기 콜레마니진딧물 1회 방사 후 2주만에 밀도 억제 효과를 보였다. 꽃노랑총채벌레 방제를 위해 유럽애꽃노린재를 1주간격 5회 방사한 결과 작기가 끝날 때 까지 방제효과가 지속되었다.
170.
2010.10
구독 인증기관·개인회원 무료
Mamestra brassicae nucleopolyhedrovirus-K1 (MabrNPV-K1) was isolated from naturally infected Mamestra brassicae (Lepidoptera: Noctuidae) larvae in Korea. Restriction endonuclease fragment analysis using EcoRI, PstI, and BamHI estimated that the total genome size of MabrNPV-K1 is about 150 Kb. The full genome sequences of MabrNPV-K1 were determined, analyzed and compared to those of other baculoviruses. The MabrNPV-K1 genome consisted of 152,471 bp and had an overall G + C contents of 39.90 %. Computer-assisted analysis predicted 159 open reading frames (ORFs) of 150 nucleotides or greater that showed minimal overlap. The gene content and arrangement in MabrNPV-K1 were most similar to those of Mamestra configurata nucleopolyhedrovirus-B (MacoNPV-B), including three polh, p10 and lef-8 gene homologues. The MabrNPV-K1 genome contains four homologous repeat regions (hr1,hr2,hr3,hr4) that account for 3.1% of the genome. The genomic positions of MabrNPV-K1 regions hr1– hr4 are conserved with the genomic positions of MacoNPV-B hr1–hr4. This indicates that the position of MabrNPV–K1 hrs is conserved with regard to both the upstream and downstream genes. Given that hrs share higher similarity within a virus strain than any hrs between species, this evidence further indicates that hrs play a fundamental role in viral life cycle and replication process appears to be tightly linked to functional conservation. The dot plot analysis, percent identity of the gene homologues and a phylogenetic analysis suggested that MabrNPV-K1 is a Group II NPV that is closely related to MacoNPV but with a distinct genomic organization.
171.
2010.10
구독 인증기관·개인회원 무료
Jae Young Choi, Yong Wang, Jong Yul Roh, Xueying Tao, Qin Liu, Jong Bin Park, Soo Dong Woo, Byung Rae Jin, Yeon Ho Je
Rice black-streaked dwarf virus (RBSDV), a member of the genus Fijivirus within the family Reoviridae, is the causative agent of maize rough dwarf and rice black-streaked dwarf diseases, both of which can lead to severe yield losses in east Asia. Although molecular approaches such as RT-PCR have potential for detection and diagnosis of this virus infections, their impact on high throughput certification is still limited. Therefore, the development of an antibody-based assay for rapid and effective diagnosis of RBSDV is preferable. In this study, we collected RBSDV from rice with rough dwarf disease and its complete nucleotide sequences of 10 genomic segments encoding 12 non-overlapping ORFs were determined. Among 12 ORFs, ORF1, 2 and 12 showed high level of similarities with the RdRp, major core protein and major outer shell protein, respectively. These ORFs were expressed as polyhedrin fusion protein or full-length soluble protein using baculovirus expression system for the preparation of specific antibody against RBSDV, which could be useful for the detection and diagnosis of this virus.
172.
2010.10
구독 인증기관·개인회원 무료
Young Moo Choo, Kwang Sik Lee, Hyung Joo Yoon, Bo Yeon Kim, Mi Ri Sohn, Jong Yul Roh, Yeon Ho Je, Nam Jung Kim, Iksoo Kim, Soo Dong Woo, Hung Dae Sohn, Byung Rae Jin
Bee venom contains a variety of peptides and enzymes, including serine proteases. While the presence of serine proteases in bee venom has been demonstrated, the role of these proteins in bee venom has not been elucidated. Furthermore, there is currently no information available regarding the melanization response or the fibrin(ogen)olytic activity of bee venom serine protease, and the molecular mechanism of its action remains unknown. Here we show that bee venom serine protease (Bi-VSP) is a multifunctional enzyme. In insects, Bi-VSP acts as an arthropod prophenoloxidase (proPO)-activating factor (PPAF), thereby triggering the phenoloxidase (PO) cascade. Bi-VSP injected through the stinger induces a lethal melanization response in target insects by modulating the innate immune response. In mammals, Bi-VSP acts similarly to snake venom serine protease, which exhibits fibrin(ogen)olytic activity. Bi-VSP activates prothrombin and directly degrades fibrinogen into fibrin degradation products, defining roles forBi-VSP as a prothrombin activator, a thrombin-like protease, and a plasmin-like protease. These findings provide a novel view of the mechanism of bee venom in which the bee venom serine protease kills target insects via a melanization strategy and exhibits fibrin(ogen)olytic activity.
