본 연구는 자일로올리고당의 과학적이고 체계적인 표준 화된 시험법을 마련하여 다양한 제형의 제품에 적용하고 자 하였다. 최적화된 시험법을 마련하기 위해 초음파 처 리 시간, 산 가수분해 시간 및 농도를 검토하여 전처리 방 법을 비교 평가하였으며, HPLC-UVD를 이용하여 시료 중 의 자일로올리고당을 분석하였다. 분석 시 1-phenyl-3- methyl-5-pyrazoline (PMP)으로 유도체화하고, photo diode array (PDA)가 장착된 high performance liquid chromatography (HPLC) (Nanospace SI-2)를 사용하였으며, 칼럼은 Cadenza C18 (4.6 × 250 mm, 3 μm)이었으며, 이동상은 pH를 6.0으 로 맞춘 20 mM 인산완충용액과 아세토니트릴을 78:22 비 율로 혼합하여 사용하였고, 0.5 mL/min 유속으로 254 nm 로 하여 분석하였다. 건강기능식품 등 시험법 마련 표준 절차에 관한 가이드라인에 따라 밸리데이션을 수행하고, 표준화된 시험법을 이용하여 유통 중인 건강기능식품 대 상 품목에 대해 시험법 적용 여부를 확인하였다. 표준화 된 시험법은 자일로올리고당을 함유한 건강기능식품 품질 관리에 대한 신뢰성을 더 높일 것으로 본다.

PURPOSES : The effect of yellow carpet visibility enhancement was verified and its impact on traffic safety for effective introduction of traffic safety facilities was studied. METHODS : There is currently no scientific evidence demonstrating that yellow carpets improve pedestrian awareness. In this study, the effect of increasing visibility caused by the installation of yellow carpet was verified, and the effect of improving the level of traffic safety was examined through an objective analysis. A drone was used to collect and analyze video data to compare the driving speeds of vehicles passing near yellow carpets at six different sites and diagnose the effects of speed reduction. RESULTS : The results showed that the visibility improvement values before and after the installation of the yellow carpet differed from place to place, but a visibility improvement effect existed in certain cases. In terms of the adequacy of the installation site, the yellow carpet had no deceleration effect on vehicles driving on signalized local streets and collector highways. It was found that there was a speed reduction effect on unsignalized local streets. CONCLUSIONS : To enhance traffic safety promotion, yellow carpets should be placed on unsignalized local streets. It is important to place the yellow carpet in the right place in a proper manner.

In this study, an evaluation system that can be used to evaluate the feasibility of developing and supplying hydrothermal energy for the operation of large-scale complex facilities was developed. To this end, this study derived factors to be considered when selecting a location for the use of hydrothermal energy using raw water from multi-purpose dams and regional water supply systems through literature survey and expert interviews. The evaluation indicators derived from this study are divided into four sectors: hydrothermal energy utilization factors, location factors, planning factors, and disaster safety factors, and are composed of 10 mid-level indicators and 34 detailed planning indicators. The relative importance of all factors was derived using the Analytic Hierarchy Process (AHP) technique, and the developed evaluation indicators and relative importance were applied to four multi-purpose dam regions in the country. As a result, it was found that in the development and use of hydrothermal energy utilizing regional raw water supply line the urban planning conditions of the supply site can have a greater impact on the location selection results than the hydrothermal energy development itself. Due to the characteristics of the evaluation indicators developed in this study and their nature as comprehensive indicators, it is believed that the results should be applied to determine the overall adequacy of site selection in the early stages of hydrothermal energy development. In the future, it is believed that it will be necessary to analyze the problems in supplying and operating hydrothermal energy using raw water from multi-purpose dams and regional water resources. Based on the analysis the evaluation system developed in this study is expected to be improved and supplemented.

본 연구는 그린커피빈추출물이 「건강기능식품의 기준 및 규격」에 추가로 등재될 경우를 대비하여 표준화된 클 로로겐산 시험법을 설정하고, 카페인이 동시 분석되도록 최적화하는 연구를 진행하였다. 최적화된 시험법을 마련 하기 위해 기기분석 및 전처리 조건을 비교·분석하여 클 로로겐산과 카페인을 30% 메탄올 추출하여 인산용액과 인산 함유 아세토니트릴으로 액체크로마토그래프를 통해 330 nm, 280 nm에서 분석하도록 시험법을 설정하였다. 시 험법 밸리데이션 결과, 직선성 정량범위 내에서 상관계수 (R2) 0.999 이상의 유의수준을 보였고, 클로로겐산과 카페 인 검출한계는 0.5와 0.2 μg/mL, 정량한계는 1.4와 0.4 μg/ mL로 나타났다. 정밀도와 정확도 결과는 AOAC 밸리데 이션 가이드라인를 통해 적합함을 확인하였고, 클로로겐 산 및 카페인 동시분석법을 최종적으로 마련하였다. 또한, 시제품과 유통제품을 통해 제형별 적용성 검토하여 클 로로겐산과 카페인을 동시에 정량 가능한 시험법임을 재확인하였다. 최적화된 시험법은 클로로겐산을 함유한 건강기능식품 품질관리에 대한 신뢰성을 더 높일 것으 로 본다.

PURPOSES : High temperatures induce excessive expansion in pavements, thus causing the closure of contraction joints between expansion joints. This results in the integration of slabs within the expansion joints into a unified slab. Compressive forces are generated owing to the friction that ensues between the unified slab and lower base layer. As the integrated slab expands and exceeds the allowable width of the expansion joint, the end restraint generates an additional compressive force. The escalating force, which reaches a critical threshold, induces buckling, thus compromising stability and causing blow-up incidents, which poses a significant hazard to road users. The unpredictable nature of blow-up incidents render their accurate prediction challenging because the compressive force within the slab must be predicted and the threshold for blow-up occurrence must be determined. METHODS : In this study, a GWNU blow-up model was developed to predict both the compressive force and period of blow-up incidents in jointed concrete pavements. The climate conditions, pavement structure, materials, and expansion joints were considered in this model. In the first stage of the model, the time at which the integrated slab expanded and surpassed the allowable width of the expansion joint was determined, and the compressive force was calculated. Subsequently, the compressive force within the integrated slab, considering both the end restraints and friction, was predicted. A large-scale blow-up test was performed to measure the blow-up force based on changes in the geometric imperfections. The measured blow-up force was adopted as the blow-up occurrence threshold, and the point at which the predicted compressive force within the slab exceeded the blow-up force was identified as the blow-up occurrence time. RESULTS : Using the GWNU blow-up model, the blow-up occurrence on the Seohean Expressway in Korea is predicted in the presence or absence of the alkali-silica reaction (ASR). Analysis is conducted using the expansion joint spacing and width as variables. As the expansion joint spacing increases, blow-up occurs sooner, and as the width increases, only the expansion joint life decreases. When applying an expansion joint spacing of 300 m and a width of 100 mm under an ASR with 99.9% TTPG reliability, the sum of the expansion joint life and blow-up occurrence time is 16 years. CONCLUSIONS : In the case of jointed concrete pavements where ASR occurred, installing an expansion joint spacing of 300 m and a width of 100 mm does not satisfy the design life of 20 years, and the expansion joint width minimally affect the blow-up occurrence time. To prevent blow-up incidents, a spacing of less than 300 m for the expansion joint is recommended. Based on the analysis results, the blow-up occurrence time and location can be predicted from the characteristics of the installed expansion joint, through which blow-up incidents can be prevented via preliminary maintenance.

본 연구는 광시성(广西省)을 사례로 중국의 아세안 국가에 대한 고등교 육 협력 정책을 분석하는 데 목적이 있다. 연구 결과, 광시성은 중국 중서 부와 해상 실크로드를 연결하는 관문이라는 지정학적인 위치를 적극 활용 해 아세안 국가를 대상으로 한 고등교육 협력을 강화하고 있다. 중국 중앙 정부는 국가 전략적으로 광시성에 해상 실크로드 전략 중 ‘민심상통’에 토 대가 되는 아세안과의 고등교육 협력 강화를 중점 과업으로 부여하고, 광 시성 지방정부는 지역이 가지는 지경학적 특성을 고려해 아세안 국가를 대상으로 고등교육 협력을 강화하고 있다. 구체적으로 보면, 광시성은 “유 학 광시”를 브랜드화해 아세안 국가 학생을 유치하고, 아세안 국가 장학금 을 신설해 지원을 확대하고 있다. 또한 중국과 아세안과의 교역 관계와 산 업 분업구조가 심화함에 따라 아세안을 대상으로 한 직업교육을 강화해 일대일로 구축에 필요한 기술 인력을 양성하고 있다. 즉, 광시성의 아세안 국가와의 고등교육 협력은 자국 고등교육의 국제화 수준을 제고하는 한편 중국과 아세안과의 산업 분업구조에서 필요한 산학 인재를 육성하는 전초 기지로 역할하고 있다. 나아가 아세안과의 ‘교육 공동체’ 구축을 강화하고 있어 ‘중국몽’의 토대가 되는 ‘교육 강국’, ‘인류운명공동체’ 구축을 뒷받침 하고 있다.

In this study, we report the microstructural evolution and shear strength of an Sn-Sb alloy, used for die attach process as a solder layer of backside metal (BSM). The Sb content in the binary system was less than 1 at%. A chip with the Sn-Sb BSM was attached to a Ag plated Cu lead frame. The microstructure evolution was investigated after die bonding at 330 °C, die bonding and isothermal heat treatment at 330 °C for 5 min and wire bonding at 260 °C, respectively. At the interface between the chip and lead frame, Ni3Sn4 and Ag3Sn intermetallic compounds (IMCs) layers and pure Sn regions were confirmed after die bonding. When the isothermal heat treatment is conducted, pure Sn regions disappear at the interface because the Sn is consumed to form Ni3Sn4 and Ag3Sn IMCs. After the wire bonding process, the interface is composed of Ni3Sn4, Ag3Sn and (Ag,Cu)3Sn IMCs. The Sn-Sb BSM had a high maximum shear strength of 78.2 MPa, which is higher than the required specification of 6.2 MPa. In addition, it showed good wetting flow.

Plastics are widely used in industries in human society and because of their structural stability, degradation is a serious global issue. To estimate the degradation of plastic, 31 edible mushrooms were cultured with the selected plastic films (polyethylene [PE], polystyrene [PS], and poly(ethylene terephthalate) [PET]) for 3 months at 25 °C. Measuring the weight of the films showed that four species of mushrooms, namely Porostereum spadiceum, Ganoderma lucidum, Coprinellus micaceus, and Pleurotus ostreatus, exhibited the highest degrees of plastic degradation. In addition, the mushrooms and fungi that exhibited the most significant plastic degradation were cross-cultured to promote this degradation. As a result, cross-cultivation of G. lucidum and Aspergillus niger showed a weight loss of 2.49% for the PET film. For the PS film, Aspergillus nidulans showed a weight loss of 4.06%. Cross-cultivation of A. nidulans and C. micaceus, which showed a weight loss of 2.95%, was noted as an alternative for PS biodegradation, but is harmful to humans. These bio-degradation effects of edible mushroom will contribute to the development of alternatives for eco-friendly plastic degradation.

As a member of ectomycorrhizal fungi, Tricholoma matsutake has a symbiotic relationship with its host, Pinus densiflora. To cultivate T. matsutake artificially, the co-cultivation of T. matsutake mycelia and bacteria from shiro was introduced. In this study, bacteria were isolated from soil samples in Bonghwa-gun, and seven bacterial isolates (B22_7_B05, B22_7_B06, B22_7_B07, B22_7_B08, B22_7_B10, B22_7_B13, and B22_7_B14) promoted the growth of T. matsutake mycelia (147.48, 232.11, 266.72, 211.43, 175.17, 154.62, and 177.92%, respectively). Sequencing of the 16S rRNA region of the isolated bacteria was performed. B22_7_B05 and B22_7_B10 were identified as Bacillus toyonensis, B22_7_B06 and B22_7_B08 as Paenibacillus taichungensis, B22_7_B07 and B22_7_B14 as P. gorilla, and B22_7_B13 as P. odorifer. These bacterial isolates were associated with the shiro community and are expected to contribute to the cultivation of T. matsutake.

Tricholoma matsutake is a traditional favorite food in East Asia, cultivated in fairy rings called “shiro,” which are found near Pinus densiflora. For effective artificial cultivation of Tri. matsutake, microorganisms from symbiotic fairy rings are co-cultivated. In this study, one bacterial isolate (Y22_B35) and two fungal isolates (Y22_F64 and Y22_F68) displayed growth-promoting effects on Tri. matsutake mycelium (158.47, 125.00, and 122.26% enhanced growth, respectively). For identification, 16S rRNA or ITS regions from the microorganisms¡¯ genomes were sequenced. Other sequences, including BenA, CaM, and RPB2 were sequenced in the fungal isolates. The bacterial isolate Y22_B35 was identified as Bacillus cereus. Y22_F64 and Y22_F68 were identified as Umbelopsis nana and Aspergillus parvulus, respectively. To identify the effects of the dominant microorganisms on Tri. Matsutake cultivation, metagenomic analyses were performed. Discovery of these Tri. matsutake mycelium growth-promoting microorganisms and metagenomics analyses are expected to contribute to our understanding of Tri. matsutake fruiting body growth and construction of biomimicry.

In this study, the expression of genes related to fruit ripening was investigated using realtime PCR. The study aimed to determine the effective conditions of calcium compounds (Calcium citrate, calcium chloride, calcium nitrate, GH-Ca, OS-Ca) and chitosan treatment to extend the shelf-life in the ‘Kumhong’ nectarine and ‘Madoka’ peach fruits. In this study, in the ‘Kumhong’ and ‘Madoka’ fruits, the expression of genes related to cell wall degradation, pectin lyase (PL), polygalacturonase (PG), and pectin methylesterase (PME), was inhibited by calcium, chitosan, or a mixture (Chitosan+CaCl2) treatment. In ‘Madoka’ peach, although the expression of the PG gene was suppressed at pre-harvest Ca and chitosan treatment, the expression of the PL gene was induced at pre- and post-harvest Ca and chitosan treatment compared to the control. The spray of calcium, chitosan itself, or a mixture (Chitosan+ CaCl2) on the trees at the ripening stage and harvested fruits can extend the shelf-life by suppressing the expression of cell wall degrading enzymes genes (PL, PG, PME) in ‘Kumhong’ nectarine and ‘Madoka’ peach tree. These results provide valuable information for the development of technology for the extension of the shelf-life of peach and nectarine fruits.

This study addresses the environmental impact associated with waste management and natural aggregate production. It explores the potential of utilizing Coal Bottom Ash (CBA) and Reclaimed Asphalt Pavement Aggregate (RAPA) as complete replacements, respectively, for fine and coarse aggregates in concrete. Despite their similarities to natural aggregates, CBA and RAPA often end up in landfills. Laboratory tests were conducted, revealing satisfactory performance in drying shrinkage and air void parameters. However, while the flexural strength met design requirements, the compressive and splitting tensile strengths were lower than predicted. The deviation in strength development behavior from natural aggregate concrete (NAC) was attributed to weak agglomerated aggregates in RAPA and the large size of the interfacial transition zone (ITZ) due to the old asphalt coating surrounding RAPA. To enhance the strength behavior, two methods were employed: compaction in the form of roller-compacted concrete and RAPA abrasion carried out by rolling RAPA in a concrete mixer. Compaction improved aggregate interlock, while RAPA abrasion decreased agglomerated aggregates and minimized asphalt coating, reducing ITZ size. These treatments resulted in improvements in compressive, flexural, and splitting tensile strengths, with the combination of both treatments having the most significant effect. Analysis of relationships between flexural, splitting tensile, and compressive strengths indicated that CBA and RAPA concrete behaved more similarly to NAC after the treatments. This research suggests that with appropriate interventions, it is feasible to utilize CBA and RAPA in concrete, contributing to sustainable construction through improved waste management, carbon footprint reduction, and conservation of natural resources.