A case of chronic osteomyelitis caused by prolonged intake of bisphosphonate showed multiple recurrences involving extensive area of mandibular body. After saucerization the removed bony fragments were decalcified, microsected in 4 ㎛ thickness, and stained with hematoxylin and eosin, Masson trichrome, von Gieson, and periodic acid Schiff reaction. The inflammatory lesion contained fragile osteophytes easily propagated into sequestra. Histologically, this osteomyelitis was relatively less suppurative but almost granulomatous, highly infiltrated with small round cells and macrophages. The osteophytes were frequently deposited on the old lamellate bone, but their ossification was extremely immature and frequently filled with sclerosed collagen bundles positive for von Gieson stain. In the polarizing microscope observation under Masson trichrome stain the newly deposited osteophytes were lack of birefringence image of Haversian system contrast to the old bone nearby. Therefore, we presume that the prolonged intake of bisphosphonate may induce the immature osteophytes lack of Harversian system, which are partly filled with sclerotic collagen bundles, and the immature bone is easily undergone extensive degeneration and necrosis, resulted in the inflammatory foci for multiple recurrent osteomyelitis.

Although the sparganosis involving soft tissues, i.e, tongue, cheek, etc., has been frequently reported, the mandibular involvement of sparganosis is not reported up to date. We present a case of intraosseous sparganosis involving whole mandible, which was clinically diagnosed as chronic osteomyelitis. After surgical operation of saucerization for the treatment of chronic osteomyelitis the removed specimens were pathologically examined and finally turned out intraosseous sparganosis. Radiological findings showed irregular multiple radiolucencies in round to ovoid shape throughout both mandibular body areas, of which peripheral rarefying radiopacity was less remarkable compared to the ordinary osteomyelitis. However, the radiolucencies of periapical granuloma, #34-36, were closely associated with the osteolytic lesions of mandibular body. Pathological examination showed a tunnel like space for the passage of sparganum larva, and heavy infiltration of eosinophilicleukocytes. And more, the parasitic tegument materials were found admixed with eggs in the granulomatous lesion, which were gradually degraded and resolved. Taken together, we presumed that the mandibular inflammatory lesion was primarily involved with sparganosis and secondarily aggravated by the periapical infection of #34-36.

The polarizing images of hard tissues including bone and cementum show characteristic features of different birefringence fortheir microstructures. Nevertheless, the clear mechanism of the amplified birefringence under polarizing microscope has not been well understood. We hypothesized that the unique polarized light could be accumulated in the microtubules due to the decreased refractory angle by the inside lower-density matrix, and then the accumulated light in the microtubules could be dispersed brightly. In order to elucidate the polarizing effect on the microtubules, the dentinal tubules in different conditions were observed, and compared with each other to explain their birefringence phenomena. In the decalcified sections of normal tooth, the dentinal tubules located near the pulp chamber showed strong birefringence, while the sclerosed dentinal tubules near the dentino-enamel junction did not show the birefringence. The birefringence was more conspicuous in the longitudinal sectionsof dentinal tubules than in the cross sections. In the decalcified sections of complex odontoma, which produced abnormal and immature dentinal tubules, the birefringence was not observed in the shrunken dentinal tubules filled with dense materials, while the peritubular matrix showed clear birefringence. The birefringence was also observed in the collagen fibers in the connective tissue, and continuously strong in the immature cemental materials containing precollagen fibers. However, the highly mineralized osteodentine did not show the birefringence. Taken together, these data suggest that the microtubules composed ofless-dense matrix than the background tissue, i.e., dentinal tubules, Haversian canals, etc., produce the amplified birefringence by the polarizing light according to the hypothesis of microtubule refraction.

53 years old female showed repeated ulceration of labial gingival mucosa at upper and lower anterior teeth, which was a partly desquamated and erythematous lesion. The lesion was slightly extended into vestibule and buccal mucosa in oral cavity, but the similar lesion was not found in other organs by medical inspection. The incisional biopsy including the border of the ulcerated mucosa and normal mucosa showed a severely inflamed mucosa, of which epithelium was gradually detached from the underlying conective tissue, so that it was diagnosed as a mucous membrane pemphigoid (MMP) pathologically. The epithelium was thinned, almost lost its rete pegs, and the basement membrane was completely distorted by the epithelial detachement. The inflammatory cell infiltration was mainly composed of small round cells and plasma cells. Immunohistochemistry was performed to know the expression of pathogenetic proteins using antisera of Igk, E-cadherin, laminin a5, elafin, and eIF5A. The basement membrane at the epithelial detachment was condensely positive for Igk, and the involved epithelium became atrophic but showed consistently positive reaction of matrix proteins and protein translation factor, i.e., E-cadherin, laminin a5, elafin, and eIF5A similar to the adjacent normal mucosa continuous to the MMP lesion. The Igk was also diffusely deposited on the basement membrane of nearby normal mucosa. Many plasma cells infiltrated around the lesion were strongly positive for Igk in their cytoplasms. Therefore, we suggest that the MMP be characterized by the deposition of Igk on the basement membrane of the detached epithelium in the absence of no other pathognomic changes of molecular events.

Most of mucous retention phenomena occurred in the mucous secretory glands, 1 e ‘ minor salivary gland, sublingual gland and submandibular gland. The mucous retention cyst from parotid gland has been very rarely reported in the literature As the parotid gland is composed of serous acini, the serous saliva is less likely to produce the retention phenomenon in the ducts or extraductal tissue. Here, a case of mucous retention cyst in parotid gland was demonstrated The patient of this case has been complaining of recurrent swelling of right cheek in parotid gland a'rea, and showed a feature of chronic sialadenitis or benign salivary gland tumor. The extravasated serous saliva was diffusely dispersed into the adjacent connective tissue, forming an ill-defined cyst cavity with hyalinized sclerotic luminal surface The inflammatory reaction was relatively mild compared to the extensive destruction of periglandular connective tissue However, the typical foamy macrophage was not seen but the infiltrated macrophages containing abundant eosi nophilic cytoplasm. The mucous retention phenomenon could be very rare in parotid gland, which is also easily distinguishable from chronic sialadenitis or benign tumor through path이ogical examination.

35 peri-implantitis recently referred for 10 years showed four types of inflammatory lesions, such as mild granulomatous lesion(n=5), severe granulomatous lesion(n=4), severe inflammatory fibrous scar tissue(n=15), severe abscess formation(n=11). However, the inflammatory lesions were usually localized at the peri-implant area accompanying compensatory hyperplasia of fibrous connective tissue. The fibrous scar and the necrotic abscess frequently occurred depend on the severity of inflammatory reaction. Among 30 cases of severe inflammatory lesions, only 2 cases involved condensing osteitis in adjacent alveolar bone. Thus, we suppose that the inflammatory progression of peri-implantitis could be partly inhibited by the hyperplastic fibrous stromal tissue stimulated by implant material. And more, the focal abscess formed around the implant can be easily drainaged through the fibrous tract of implant pathway, resulted in the chronic persistent inflammatory granulomatous lesion, that is contrast to the common socket granuloma after tooth extraction. However, depend on the degree of inflammatory reaction in the peri-implantitis the inflamed fibrous collagenous tissues, unregenerated graft materials, necrotic abscess and sequestra should be removed by surgical intervention and followed by antibiotic therapy, because the peri-implant tissue is as vivid as the normal periodontium for the inflammatory defense system. Therefore, we suggest that the inflammatory lesions of peri-implantitis be carefully treated to improve the prognosis for the following dental treatments

In order to perform the protein analysis using the paraffin sections previous fixed with formalin, we applied the ImmunoMemBlot (IMB) method1) to detect the epitopes of target proteins with specific antibodies. In this study the protein extracts were obtained from the paraffin sections of each representative case of ameloblastoma, adenomatoid odontogenic tumor (AOT), and normal gingiva, and more a protein extract from fresh tissue of ameloblastoma was also compared to evaluate the IMB results used with 24 different antibodies. First of all, in the comparison between the paraffin section extract and fresh tissue extract of ameloblastoma, the latter consistently showed more positive IMB reaction than the former. Meanwhile, the paraffin section extract of ameloblastoma was more comparable with that of normal gingival, disclosing that most of proliferating genes, oncogenes, and apoptosis related genes, i.e., PCNA, CDK4, c-erbB2, CEA, p53, Bax, Bad, FLIP, FAS, Bcl-2, p21, N-ras, MMP-2, MMP-9, caspase-3, -8, -9, were highly expressed in ameloblastoma, but EGFR, HGF, and VEGF were similarly expressed both in the ameloblastoma and in normal human gingiva. On the other hand, the comparison between ameloblastoma and AOT both in the immunohistochemistry and IMB using their paraffin section extracts clearly demonstrated that the ameloblastoma showed more expression of proliferating genes and oncogenes while the AOT showed more expression of apoptosis related genes, i.e., Bax, Bad, FLIP, and caspase-9. Taken together, these data suggest that the IMB can be used for the primary screening of quantitative protein analysis using the paraffin section extract, and that the IMB results could be evaluated in conjunction with the immunohistochemical observation.

Immuno-MemBlot is a technique for detecting, analyzing, and identifying proteins, similar to the Western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular or slot templates directly onto the membrane. Recently we developed a new Immuno-MemBlot (IMB) method applying immunoreactions and coloring procedures directly in the wells of MemBlot apparatus, which were connected by canals to perform drainage for reagent application and buffer irrigation. This IMB method was designed to get theimmunoblot results more rapidly and clearly than the previous immunoblot ones. This study is aimed to evaluate the analytical accuracy of IMB using different biological assay. In the sensitivity test of IMB the monoclonal antibody can clearly detect the 30 ng (about 12 pM) of Mucocidin peptide (35 mer), and is also available to detect at least 10 ng (about 4 pM) of Mucocidin peptide (35 mer). The IMB was effective in the quantitative analysis of methothrexate (MTX) assay for cellular apoptosis. And more, this IMB is useful to screen large number of specific samples with ease and accuracy in a short time. In the screenings for the presence of Mucocidin in saliva the quantitative comparison is conspicuous among 48 persons depend on the different conditions ofgender, drinking and smoking habits, and oral diseases. Therefore, it is presumed that, even though the target proteins were partly degraded, a specific epitope can be detected if a monoclonal antibody was still reactive. Conclusively, these data suggest that the IMB can be useful in the primary qualitativeand quantitative analysis of proteins in various fluids, i.e., blood, saliva, tear, urine, etc.

In order to obtain novel genes related to the human craniofacial development, molecular cloning and sequencing, and in situ hybridization using craniofacial tissue sections were performed and followed by protein structure simulation. Totally 231 clones were obtained from the subtracted craniofacial tissue cDNA library of human embryo. Random cloning using the non-redundant clones from the craniofacial tissue of human embryo was done and obtained 398 clones from the premade human chondrocyte cDNA library. Their partial sequence data showed that 214 clones of subtracted cDNA library of craniofacial tissue were still non-redundant in Genebank search. And 20 clones among 498 clones of premade chondrocyte cDNA library were known to be undefined genes. Through in situ hybridization screening in the craniofacial tissue sections of 10 weeks old human embryo 36 clones were found to be positive in specific tissues. Depending on the cell types of sirnilar developmental origin, the positive reactions could be divided into five groups. Among the 20 clones of undefined genes from human chondrocyte cDNA library, 7 clones showed characteristic positive reaction in human cartilage tissue by in situ hybridization. From the simulated protein structure, motif analysis and in situ hybridization studies for the 7 undefined clones, Ch89, Ch96, Ch129, Ch285 clones may function in the outer space of the cell constituting a part of matrix protein complex, and Ch276 as a transmembrane protein which might partic ipate in matrix calcification around chondrocytes. Ch153 is a kind of antirnicrobial protein also acting as an inflammation mediator, and Ch334 clone is a zinc finger protein, of which expression increases in human adult tissues We presume these novel genes from human chondrocytes may provide a new path of chondrocyte development and functions of human craniofacial tissues

The fragile X mental retardation (FMR) syndrome is the largest source of inherited mental retardation. The syndrome is usually caused by the transcriptional silencing of fragile X mental retardation gene (FMR-1). An 18 years old male wascompla띠ing of multiple toαh missing and abnormal facial profùe, of which signs were matemally dominant in his family. In the C)π。gene디c analysis the pa디ent and his parents did not show any discontinuity in the long arm end of X chromosome, but in the PCR produαs targeω19 the CGG repeat sequence in the 5' untranslated region of FMR gene both the patient'’ s and his mother' s gDNAs produced a normal and an extra bands, sized about 400 and 800 bps, respectively, while the his father' s gDNA produced only one normal band, sized about 400 bps. 까1US ， we suppose that the pa디.ent has heterozygotic alleles of FMR gene inherited matemally, and that the patient s FMR gene was in a premutated state relevant to the dentofacial abnormalities.

As pulp calcification occurs at least fifty percent of total teeth, the focal calcification in pulp chamber usually appears in all age groups. However, the pulp calcification is one of the important pathologic changes affecting the pulp vitality. In order to elucidate the mechanism of pulp calcification during the retrogressive degeneration of pulp tissue we performed an immunohistochemical study for proteases (MMP-3, MMP-10, and cathepsin-G), antiproteases (TIMP-1, α1- AT) and proteins involving tissue protection (TGase-2 and HSP-70). In the normal pulp tissue MMP-3 and MMP-10 were weakly expressed, but cathepsin-G and TIMP-1 were rarely expressed. Around the calcifying tissue of MMP-3, MMP- 10, and α1-AT were predominant, but TIMP-1 and cathepsin-G were sparsely expressed. On the other hands, TGase-2 and HSP-70 were condensed in the proximal fibrous tissue. These data suggest that the pulp calcification is related to retrogressive pulp degeneration, which could be resulted in the incomplete digestion of the degenerated stromal tissue by different proteases. We presume that the aberrant protease digestion of chronic pulpal pathosis, i.e., sclerotic fibrosis, chronic pulp degeneration, etc., may enhance the dystrophic calcification in dental pulp.

최근 지표수의 취수원 다변화 및 수질에 대한 불신 등으로 강변여과 개발에 관심이 모아지고 있다. 선진국에서의 강변여과 개발은 150년 정도의 역사를 가지고 있으며, 우리나라에서도 지속적으로 증가하고 있는 용수수요에 안정적으로 대처하기 위한 원수 확보방안으로 강변여과에 대한 조사가 1990년대부터 시작되었고, 현재 낙동강을 중심으로 몇몇 지자체에서는 강변여과를 활용한 상수도 공급이 이루어지고 있다. 그러나 지금까지는 강변여과에 의한 개발가능량 산정과 관정

한국에서는 증가하는 용수수요에 대처하기 위해 1990년대부터 낙동강 유역의 지자체들에서 강변여과수를 활용하고 있다. 본 연구에서는 서울의 원수수질 안정을 위한 방안으로 강변여과 도입의 타당성을 검토하였다. 선행 논문에서 계층분석과정(AHP)에 의해 선택된 광나루지구에 대한 개발가능량 평가를 위해 지하수 모델링이 수행되었다. 광나루지구에서는 생태계보존지역 등을 고려하여 하천부지 약 1,200m 구간에 대해 관정시스템을 구축할 수 있을 것으로 파악되었다.

선진국에서의 강변여과수 개발은 150년 정도의 역사를 가지고 있다. 한국에서도 지속적으로 증가하고 있는 용수수요에 안정적으로 대처하기 위한 원수 확보방안으로 강변여과수에 대한 조사가 1990년대부터 4대강 유역을 중심으로 시작되었으며, 현재 낙동강을 중심으로 강변여과를 활용한 상수도 공급이 이루어지고 있다. 본 논문은 서울의 원수수질 안정을 위한 방안으로 강변여과 도입의 타당성에 대한 연구이다. 개발 적지의 선정을 위해 여러 가지 속성들을 계층적으로 분

강변여과는 강물을 강변의 충적대수층에 통과시켜 지층의 자체정화능력을 이용하여 오염물질을 상당량 저감시킨 후 양수하는 방식이다. 국내에서는 대부분의 원수를 지표수에서 취수하여 사용하고 있는 실정이나, 오염물질의 증가로 인하여 지표수를 원수로 사용하기가 점점 어려워지고 있다. 강변여과를 이용하여 양질의 원수를 확보하기 위해서는 오염물 이동에 관한 이해가 중요하다. 본 연구에서는 대수층에 용존성 유기물질(DOM)과 박테리아가 동시에 존재할 경우를 대상으로 대

강변여과는 원수를 강변의 대수층에 통과시켜 지층의 자체 정화능력을 이용하여 원수 중의 오염물질을 상당량 저감한 후 상수를 공급하는 방식이다. 본 연구에서는 지질정수를 추정하는 방법 중 하나인 역해석을 이용하여 강변여과 지역의 투수량계수를 추정하였다. 역해석이란 현장에서 직접 측정한 값과 프로그램을 통해 계산된 값의 차를 목적함수로 두고 이를 최소로 하도록 미지의 매개변수를 결정하는 것이다. 최적화 기법으로 BFGS(Broyden-Fletcher-Gold