전통 발효 식품인 된장에 새로운 기능성을 부여하기 위한 기초실험으로서 된장을 제조하여 일정기간 발효시킨 후, 인삼 농축액을 일정 비율로 첨가하여 된장 발효 중의 미생물, 효소 활성 변화와 주요 성분의 변화를 조사하였다. pH는 발효기간 내내 감소하였으며, 총산은 발효기간이 길어짐에 따라 증가하였다. NaCl함량은 발효기간이 길어짐에 따라 증가하였다. NaCl 함량은 발효 30일 까지는 15.67~16.90%로 증가하는 경향을 나타내었으며, 아미노

The variation of microorganisms (activated sludge, Saccharomyces cerevisiae, Aureobasidium pullulans) caused by the biosorption of Pb2+ was observed by TEM and microscope. By the TEM observation of S. cerevisiae, the plasmolysis and lysis of cell wall or cell membrane were occurred by the penetration of Pb2+ into the inner cellular region. However, in the case of A pullulans, the plasmolysis and lysis of cell wall or cell membrane were not occurred because of the prevention of Pb2+ penetration by the extracelluar polymeric substances (EPS). A flocculation of microorganisms, in the case of A. pullulans, was observed by the Pb2+ accumulation after 3∼4 h and the color was changed from white to black after 1 day. The flocculation of activated sludge was improved by the accumulation Pb2+ after 1 h, however, the floc was broken up and the settling efficiency decreased after 1 day.

115 종의 생약재 중에서 식품부패성 및 병원성 미생물을 대상으로 항균활성을 나타내는 생약자원을 검색한 결과 식품부패성 세균 Bacillus subtilis에 강한 항균활성을 보인 것은 단삼, 관중, 가자, 독활, 파고지, 오미자이었으며, 식중독균인 효모 Candida albicans IFO6258에 대해서는 지모와 관중 두 생약재가, 식품부패성 효모 Schizosaccharomyces sp.에 대해서는 지모, 관중, 원지가 각각 강한 항균 활성을 보였다. Streptococcus mutans에 대해서는 관중, 단삼, 소목, 방기, 오미자, 오배자, 고삼, 목향이 강한 항균 활성을 보였다. 피부 각층 및 모발에 침입하여 피부병을 야기하는 백선균 Trichonphyton mentagrophytes에 대해서는 지모, 강황, 목향, 호장근, 지유, 황금, 가자들이 항균활성을 나타내었다 . 항균spectrum이 넓으면서 비교적 항균활성이 강한 관중, 단삼, 가자, 황금에 항균활성이 강한 생약자원인 가자, 계피, 방기, 황금등의 생약재를 각각 1 : 1로 혼합한 추출물에 대한 항균활성을 조사한 결과 모든 혼합 추출물에서 단독 추출물 보다 강한 항균활성을 나타내었다.

자소잎중 물과 에탄올을 이용하여 얻은 추출물을 2종의 부패곰팡이(Aspergillus flavus KCTC 6143, Aspergillus jlavus KcrC 6961) 및 5종의 식중독세균 ( isteria monocytogenes ATCC 15313, Staphylococc따 aureus 196E ATCC 13565, Escherichia coli 0157:H7 ATCC 43895, Salmonella typhimurium ATCC 1331

In order to study the natural preservation of food, sixteen kinds of medicinal plants were extracted by 80% methanol and then antimicrobial activity of the extracts was investigated against various kinds of microorganisms. The extracts of medicinal plants showed good effects of antimicrobial activity against bacteria except Bacillus cereus, Escherichia coli O-157. Especially, the extracts of Artemisia annua, Aster scaber, Solidago virganrea shower good inhibitory effects on the growth of bacteria to the cooling preservation foods, and Artemisia annua was decreased of the TMA(Trimethylamine) test. the result thus indicate that Artemisia annua, Aster scaber, Solidago fischeri extract inhibitory effects on cooling preservation foods, so that it may be potential as a natural preservatives.

전통민속주 제조에 널리 사용되어온 국화꽃잎에서 에탄올 추출하여 식품부패에 관련된 미생물을 대상으로 항균활성을 검색하고 에탄올 추출물의 농도별 항균활성과 최저 생육저해 농도를 조사하였다. 국화 에탄올 추출물의 항균활성은 산국이 B. subtilis에 대해 항균활성이 높게 나타난 반면, 소국은 E.coli에 대한 항균활성이 산국보다 다소 높게 나타났다. 8종의 부패균에 대한 에탄올 추출물의 항균활성은 산국이 소국에 비하여 높게 나타났으며, 산국 에탄

부패하는 두부에서부터 각종 미생물 18종을 분리하여 동정한 결과, B. cereus, X. luminescens, A. calcoaceticus, Kl. pneumoniae, S. typhimurium, Pantoea sp., Bacillus sp., Cardiobacterium sp., E. coli, St. aureus, Yersinia sp. 등 11종의 각기 다른 미생물로 나타났다. 실온에서 두부의 부패 과정중 이들 미생물의 존재 함량은

This paper discussed about the effect of Zoogloea ramigera on the initial microorganism attachment and the biofilm growth. The additions of 5, 10 and 15%(w/w) of Zoogloea ramigera were facilitated for the initial attachment on the surface of the acryl disk. At biofilm growth, the more Zoogloea ramigera added to the activated sludge, the more biofilm dry weight was obtained. In order to get the stable biofilm and to minimize the start-up periods, initial biofilm formation using activated sludge with floe forming microorganisms like Zoogloea ramigera was recommended rather than that without floe forming microorganisms.

In order to develop of support media for biofilm reactor, physicochemical properties and attachability of surface of activated carbon, clay mineral, non-clay mineral, and waste mold sand were examined. Measured physicochemical properties of materials were surface roughness, mean particle size, surface area, hydrophobicity, and surface charge. At a tested materials, activated carbon was the best attachable material and microorganisms were attached 20.1 × 10^7 CFU/㎠ at surface, compared with diatomaceous earth which were attached of 9.2 × 10^7 CFU/㎠. In our research, surface area and hydrophobicity showed more influence than any other factor on attachment of microorganisms.

This paper discussed effect of the surface roughness and the hydrophobicity of support material on the microbial attachment in a rotating biological contactor. The hydrophobicity of each support material was determined by the measurement of contact angle of water and the surface roughness was measured by the surface roughness instrument. Microorganisms have well attached on the surface of more hydrophilic support material like Nylon6 than that of the hydrophobic support material like PE. When the relatively hydrophilic surface was roughen, the microbial attachment was increased but when the relatively hydrophobic surface was roughen, the attachment was slightly increased because the hydrophobicity of support material was increased by roughening the hydrophobic surface. Although both variables, the surface hydrophobicity and the surface roughness, have influenced the microbial attachment, the influence of the surface roughness overruled that of the surface hydrophobicity. Support material whose surfaces were roughened about l㎛, 6㎛ and 11㎛ were allowed for attached 3, 7 and 24hr, but the differences of maximum and minimum attachment of each material gave nearly constant values and similar trend with time.