Substances associated with the regulation of food intake rate leading overweight, obesity, were reviewed for their roles in the body weight control pathway. Feedback mechanisms in food intake were reviewed focusing on the driving force of eating motivation. Definition of hunger and fullness along with virtual food developed by Chun(2002) was discussed. Effective hunger was defined on the basis of virtual food. Relationship of consciousness elements with food emotion and evaluation were reviewed with food frequency curve of subjects experimentally obtained with cooked rice and potato flake. Clustering analysis of consciousness effect showed roles of the layers of consciousness,

In order to polish complicated-shaped inner surfaces of models, a new polishing method with magnetic congelation liquid was invented, we called this name MAGIC polishing tool. This tool is magnetic fluid at high temperature, can be solidified by cooling. The distribution of abrasives in the liquid can be controlled by magnetic field. Therefore we can make a special polishing tool which has well arranged abrasives inside after cooling. In order to know the fundamental polishing properties of this new polishing tool, brass plate was polished. The polishing tool was pressed and rotated against the brass plate in specified polishing time. After many experiments, it was found that this type of polishing tool provided enough removal rate and surface roughness.

Since sustenance and abolition of death penalty system have an argument about the world view, we cannot conclude which one is right. The death penalty system can be changed by the age and situation. In Korea, the death penalty system is based on the situation of its application and Korean people's mind of law. So we should investigate the death penalty system in terms of fluctuating the two factors. In the first stage, the death penalty system has to be reformed with respect to reduction of the death penalty regulation, establishment of the criteria in death penalty application, and elaboration of criminal suit procedures. In the second stage, the system of death penalty suspension should be introduced as a transitional one to abolish death penalty. This is the necessary system from the viewpoint of humanism and criminal policy. It is also essential to avoid misjudgement. This system has a five-year deferral period, in which after five-year suspension, we will determine whether death penalty is executed or penal servitude for life is served. In the final stage, when it is legally convinced that people in Korea reach a consensus to abolish death penalty, we should introduce special penal servitude for life, which is more strict than the present penal servitude for life. Moreover, we should reform regulations of criminal law to be able to assign penal servtude for life as well as special one to criminals.

콘돼트렌즈 관리 용액의 세포독성올 검사하기 위한 방법이 여러 가지 이용되고 았 으나， 직접 소프트콘택트렌즈에 홉수된 관리용액의 독성을 조사하기는 어려운 실정이 었다. 따라서 본 연구에서는 관리용액이 홉수원 렌즈 위에 세포를 직접 배양하여 용 출된 관리용액으로 언한 세포독성을 평가하고자 시행하였다. 여러 가지 콘돼트헨즈 관련 용액 중 보존제 성분으로 가장 많이 사용되고 있쓴 Benzalkonium chloride (BAC)와 Multipurpose solutions(MPS) 빛 AOSept에 Etafilcon A(FDA공인 W 그룹， Acuvue) 재질인 소프트콘태트렌즈콜 4시간 동안 담구어 용액이 홉수되도록 한 후 배양 결막세포 (clone 1- 5c -4) 룹 콘택트렌즈 위에 직접 배양하여 2 시간 통안 용출액에 의한 세포독성을 조사하였다. 또한 같은 용액에 세포를 2시간 직접 배양하여 독성 정 도를 비교하였으며， Monolayer cell 에 같은 용액올 15분 직접 처리하여 독성올 비교 하였다. 그리고 계대 배양 시 25% 농도로 같은 용액을 세포 혼탁액에 혼합하여 부착 률과 증식률을 조사하였다. 세포독성은 세포를 계수하는 방볍과 시l 포활성올 조사하는 방법올 이용하였으며 형광염색을 통해 세포 생존용플 비교하였다. 콘택트렌즈 용출액에 의한 세포 독성은 MPS는 거의 독성이 없었으며， BAC (0,00 1%-0，α)5%) 처리군에서는 40% 의 세포 증식저해가 였으벼. BAC 0， 01% 와 AOSept (0α)()5 -0.oo1%)에서는 70%이상의 증삭저해를 나타냈다. 또한 세포를 실험 용액에 직 접 2시간 배양하여 세포뜰 계수한 결과 대조군인 식염수에 비교하변 MPS 는 40% 이상， BAC 0.001%는 70% 이상， AOSept는 80% 이상 세포 뚱식 서해가 있으며， BAC (O.α)5， 0.01%) 에서는 세포의 생존율이 없는 것으로 사료된다. MTT assay 와 LDH assay를 한 결과 세포룰 계수 하는 결과와 유사하게 나타났다. 형광염색을 이용한 생존율 조사 결 과， BAC 0.01% 처리군에서는 세포가 사멸하는 것을 확인할 수 았었다.