본 연구는 복부 초음파 검사를 통해 확인된 지방간 환자의 지방간 정도에 따른 혈액학적 고지혈증 지표와의 관련성을 분석하여 관련 연구에 기초자료를 제공하고자 시행되었다. 2017년 1월 1일부터 2017년 12월 31일까지 총 412명의 성인을 대상으로 하였으며, 복부초음파 검사를 진행하여 지방간이 확인된 환자 중 초음파 검사 시행일 기준 3개월 이내의 혈액학적 데이터가 있는 환자를 대상으로 후향적 분석을 실시하였다. 연구결과, 지방간 정도에 따른 혈액학적 수치는 중성지방, AST, ALT에서 통계적으로 유의한 차이가 있었고(p<.05), 지방간은 전체 변수 중 성별, 중성지방, AST, ALT와 관련성이 있었다(p<.05). 결론적으로 지방간 정도는 고지혈증의 혈액학적 수치와 직접적인 영향인자는 아니었지만 그 관련성 확인을 통해 지방간과 고 지혈증 동반 관리의 필요성을 확인했다.

산업의 발달로 인한 생활습관과 신체활동 부족 등으로 한국인의 비만인구가 급증하고 있다. 전산화단층 영상을 이용한 기존의 지방량 계산 프로그램에서 반자동방식의 프로그램이 사용되고 있다. 관련 문제를 해결하기 위한 방법들이 제시되고 있으나 본 연구에서는 모폴로지 연산을 이용한 알고리즘을 제시하고 절차가 간단하고 비교적 계산량이 적은 새로운 방법으로 문제를 해결하고자 한다. 모폴로지 연산을 통해 침식과 팽창을 반복한 결과 영상으로부터 문제점이 해결된 것을 알 수 있어 결과로부터 각 조직 간의 경계를 더욱 정교하게 얻을 수 있었다. LoG (Laplace of Gaussian) 함수를 이용해서 각 조직간 경계 부분을 분할하였다. 각 경계는 명확하게 구분이 되었으며, 피하지방을 계산하기 위해 충분한 정보를 제공하였다. 개발된 분할결과를 사용하여 향후 자동 지방량 계산을 할 수 있다. 정확한 분할 도구를 제공함으로써 의사에게 편리함을 주고 재검사로 인한 피폭과 검사비용을 줄이는데 도움이 될 것으로 판단한다.

본 연구는 CT를 이용한 지방측정(Fat measurement)에 반복 재구성법(IR)이 어떠한 영향을 미치는지 알아보기 위하여 기존의 필터보정 역투영법(FBP)과 비교하여 고급 모델링 반복 재구성법(ADMIRE)은 어떠한 차이가 있는지 알아보고자 한다. CT Fat measurement에 ADMIRE가 미치는 영향에 대해 알아본 결과 기존의 Filtered back projection과 비교하여 Visceral fat area, Subcutaneous fat area, V/S Ratio, Visceral fat area의 HU 그리고 Subcutaneous fat area의 HU는 통계적으로 유의한 차이가 없었다. 하지만 ADMIRE Strength가 커질수록 Visceral fat area의 Noise는 최대 12%, Subcutaneous fat area의 Noise는 최대 19% 감소하였다. 그리고 CT를 이용한 Fat measurement에서 내장지방과 피하지방 측정에 ADMIRE 알고리즘은 아무런 영향을 주지 않았다.

본 연구는 초음파상 지방간을 세 등급으로 구분하여 적용하는 것이 어떠한 임상적 의의가 있는지 알아 보고자 하였다. 2018년 6월부터 2019년 4월까지 부산 P 검진센터에서 복부초음파 검사를 받은 3607명 중 지방간으로 진단된 1047명(남 818명, 여 229명)을 대상으로 하였다. 초음파상 간 실질의 에코정도, 음향감 쇄정도, 간 내 혈관 및 횡격막이 보이는 정도에 따라 GradeⅠ군(경증 지방간), GradeⅡ군(중등도 지방간), GradeⅢ군(중증 지방간)의 세 등급으로 분류하고, 각 군별 비만지표(체질량지수, 허리둘레), 혈액학적 분석 및 대사증후군과의 연관성을 남녀로 구분하여 분석하였다. 결과로 지방간 등급이 올라갈수록 남자는 여자에 비해 중증 지방간의 비률이 높게 나타났다. 성별에서는 남자가 지방간 등급이 올라 갈수록 각 군별 평균연령이 높게 나타났다. 체질량지수, 허리둘레는 남녀 모두에서 유의하게 지방간 등급이 올라갈수록 증가 하는 차이를 나타내었다(p=.000). 혈액학적 분석에서는 남자는 AST, ALT, γ-GTP, TG, 공복혈당, 당화혈색소에서 유의한 차이를 나타내었다(p<.05). 여자는 ALT, γ-GTP, TG에서 지방간의 등급이 올라갈수록 유의한 차이가 나타났다(p<.05). 대사증후군의 유병률은 남녀 모두 지방간의 등급이 올라갈수록 유의하게 증가 하였다(p=.000). 연구결과를 기초로 초음파상 지방간을 등급에 따라 세분하여 적용함에 있어 혈액학적 변수, 대사증후군 등을 고려하여 지방간 등급을 나눈다면 지방간의 치료 및 경과관찰에 도움이 될 것으로 사료된다.

The climate-food relationship and effect of climate on staple grain crops have been studied widely. However, the relationship between climate and liquor and its characteristics are not explored sufficiently. This study presented the climate and its association with liquor over various climatic regions of mid-latitudes. There is huge diversity in vegetation and cultures due to various climates of the mid-latitudes and the manufacturing of liquor depends on the regional raw materials and methods.

The purpose of this study is to figure out the current condition of local governments (Si or Gun) to implement rural development policy in the face of the central government’s drive for increasing devolution. Since late 1990s or early 2000s when scope of rural policy began to expand quantitatively, there has been considerable increase in rural residents’ participation into community projects as a whole. Nevertheless, there exists regional differentiation in local governments’ efforts to systematically support rural development projects (CRDP: Comprehensive Rural Development Programme) and community activities by setting up intermediary organizations or hiring rural development professionals in a public office. According to this survey on local government officials, regions that have made such efforts to support rural policy at a local level, show more advanced level of institutional capacity than other regions in local decision making process for CRDP, role of strategic rural developmental plans, vitality of community actors, etc. In the era of increasing devolution, these differences can be expected to result in diverging performances of each area in regionalized rural policy. The central government needs to introduce EU’s LEADER-type rural development programmes to support community acitivities by various local actors and at the same time promote local governance building for rural policy, adopting institutional rewarding system such as rural planning contract.

중성지방(Triglyceride, TG)는 죽상동맥경화증과 같은 혈관의 만성 염증성 병변을 유발하는 인자 중 하나 이다. 종양괴사인자-알파 (TNF-α), 인터루킨-1 베터 (IL-1β)와 같은 염증성 사이토카인은 염증 질환의 주요 요인으로 염증 부위에 T 림프구, 단핵구등의 면역 세포의 침윤을 유도하거나 세포 및 조직 괴사를 일으킴으로써 질병을 더욱 악화시킨다. 본 연구에서는 혈관 염증에 관여하는 Jurkat T 림프구와 U937 단핵구에 T G를 처리하였을 때 TNF-α와 IL-1β의 발현에 미치는 영향을 조사하고자 했다. Jurkat T 세포에서 TG에 의해 TNF-α의 mRNA 발현이 증가하였고, U937 단핵구에서는 TG에 의해 TNF-α와 IL-1β 모두 mRNA 발현이 증가하였다. 또한 유도성 산화질소합성효소(inducible nitric oxide synthase, iNOS)가 TG에 의한 TNF-α와 IL-1β 의 발현 증가에 관여하는지 확인하기 위해 iNOS 억제제인 W1400을 세포에 전처리하여 iNOS의 활성을 차단하였다. 그 결과, W1400을 전처리한 세포에서는 TG에 의한 TNF-α 및 IL-1β mRNA 양이 대조군과 유사하게 낮은 수준으로 관찰되었다. 이는 혈관 내 TG의 증가가 T 림프구와 단핵구를 자극하여 iNOS 신호를 거쳐 염증성 사이토카인을 분비시키고 혈관염증질환을 발생하는데 관여하는 것을 확인시켜주었다. 결론적으로, 중성지방이 염증성 병변을 악화시키는데 있어 iNOS의 활성이 사이토카인 분비 등에 작용하며 병변을 더욱 악화시키는데 기여할 수 있다. 반면, iNOS 발현을 조절하여 고지혈증 환자의 치료에 유효한 표적 물질로 이용될 가능성이 있다고 사료된다.

Background : The purpose of this study is to select potential genetic resources from safflower germplasm collected from India based on their oil compositions and agronomic characteristics. Methods and Results : The agronomic characteristics were measured during the growing period of the safflower. Total oil contents were recovered by Soxhlet extraction and the fatty acid compositions were analyzed by using gas chromatography. The mean of plant height, leaf length and leaf width were 100.19 ㎝, 20.49 ㎝, and 7.29 ㎝, respectively. The percentage of leaf margin with serration was 95%, and 2% of the total resources didn’t have spines on the involucral bract. K185681 had no spines on the involucral bract and the plant height was the smallest. 73% of the flower of safflower was yellow. 68% of safflower germplasm changed flower color from yellow to red. Total oil contents of 267 safflower accessions showed a significant variability among the entire domain of collections and ranged from 5.81 to 38.91%. Palmitic and stearic acid were ranged from 4.98 to 6.65%, and 1.82 to 2.73%, respectively. Oleic and linoleic acid showed a wide variation which ranged from 10.53 to 22.27%, and 69.46 to 81.26%, respectively. Linolenic acid was ranged from 0.06 to 0.13%. K185639 and K185639 had the highest total oil contents and linoleic acid, respectively. Cluster analysis based on oil composition and agronomic characteristics data divided the germplasm collections into three groups. Group Ⅲ having 114 accessions contained accessions with taller plant height than the other groups. Group Ⅱ having 68 accessions, the main color of flower was white but the other groups were yellow. Oleic acid showed a strong negative correlation (r = -0.9691**) with linoleic acid. Principal component analysis (PCA) based on the oil compositions and agronomic characteristics data revealed that the first two principal components (PC1 and PC2) together explained 36.28% total variation. Conclusion : These results showed that K185681, K185639 and K185639 could be useful to develop breeding and functional food.

Background : Alpha-linolenic acid is a type of omega-3 fatty acid and has been reported to be found at a remarkably high content in seeds of perilla (Perilla frutescens). The purpose of this study was to investigate the fatty acid compositions in 45 perilla accessions collected from Russia and recommend the potential genetic resources related to their fatty acid compositions. Methods and Results : The 45 accessions of perilla seeds which were collected from Russia were used for the study. Perilla seed oil was recovered using hexane in a soxhlet extraction method. The fatty acid compositions were analyzed using gas chromatography. The total oil content was ranged between 28.39 and 46.89%. The compositions of palmitic, stearic, oleic, linoleic and linolenic acid ranged from 5.57 to 7.09%, 1.16 to 2.27%, 11.83 to 19.55%, 11.92 to 16.71%, and 59.19 to 67.28%, respectively. Cultivars 'Dayu', 'Daeyu' and 'Anyu' showed lower linolenic acid composition compared to the average value of linoleic acid in perilla germplasms collected from Russia. Cluster analysis based on the fatty acid composition of the 45 perilla accessions segregated into three groups. Group Ⅰ characterized as higher palmitic, stearic and oleic acid compositions compared to other groups. Group Ⅱ which contained 12 accessions had high total oil and linoleic acid composition. Group Ⅲ characterized as a higher linolenic acid composition compared to other groups (p < 0.001). Oleic acid showed a significant negative correlation (r = -0.825) with linolenic acid. Principal component analysis (PCA) revealed that the first two principal components together explained 74.4% total variation. Conclusion : Our results indicated that accessions IT235818, IT235820, IT226739 which exhibited high linolenic acid composition could be useful to develop new functional vegetable oil materials.

Background : Obesity is a type of metabolic diseases caused by unbalanced in take and consumption of calories. 3T3-L1 is differentiated into adipocytes by various hormones and transcription factors and accumulates intracellular lipid. Therefore, it is important to inhibit the adipocyte differentiation precess for effective obesity inhibition. Aster yomena and Aster glehui are medicinal plant of Compositea family that grows widely in Korea. Aster genus plants have been used to treat snakebite wound or bruises in oriental. The aim of this study was comparison of inhibitory effect oxidation and adipocyte differentiation with Arial parts of A. yomena (AY) and A. glehni (AG). Methods and Results : AY and AG were cultivated from Pyeongchang in Korea, 2018. AY and AG were extracted by 70% ethanol (-E) and water (-W) at room temperature. AG-W has higher phenolic content (6.92 ± 0.23) and AG-E has higher flavonoid content (8.22 ± 0.19) than other extracts. AG-E has higher radical scavenging activity on DPPH and ABTS assay (IC50 value; 104.88 ± 10.50 and 30.06 ± 0.27). In cytotoxity assay, all extracts concentrations of lower 100 ㎍/㎖ were nontoxic to the cells and can be applied for the next assay. The anti-adipogenic effect of extracts were determined in 3T3-L1 cell by Oli Red-O (ORO) staining. The lipid diplot stained with Oil red O was dissolved to determine by microplate-reader. AG-W significantly reduced the adipocyte differentiation of 3T3-L1 cells (70.49%) compared with other extracts (AG-E, AY-W and AY-E). Conclusion : Theses results reveal that the water extract of AG has utility as a functional food material for preventing obesity.

천연물 유래 단일 성분의 지방세포로의 분화 및 지방축적 억제효과에 대한 잠재성을 탐색하기 위해 본 연구에서는 화살나무의 껍질에서 분리 정제한 FG가 3T3-L1 지방전구세포에 있어서 지방세포로의 분화 및 지방구 축적에 미치는 영향에 대해 평가하고, 관련 메카니즘에 대해 검토하였다. FG는 분화용 배지에 포함된 분화 촉진인자들의 자극에 의한 지방생성에 있어 주요한 전사인자인 PPARγ와 그 표적 단백질인 FABP4의 발현을 억제함으로써 지방세포 분화 및 지방구 축적을 억제하는 것으로 나타났다. 이상의 결과로부터 FG가 비만이나 비만과 관련된 당뇨병 등을 예방, 개선하는데 유용한 물질로 사용될 가능성이 있을 것으로 생각된다.

비알코올성 지방간 질환은 만성 간질환의 가장 흔한 원인이다. 이 연구의 목적은 비알코올성 지방간 질환 동물모델을 확립하고 생체 내 짧은 양성자 자기공명분광법을 이용하여 비침습적으로 간의 대사물질과 지질의 변화를 정량 분석하고자 하였다. 각 실험은 고지방식 먹이를 먹고 나서 0주를 기준선으로 하고, 2, 4, 6, 8주에 쥐의 간 실질 조직으로부터 측정되었다. 0주를 기준선으로 하고 2주와 비교했을 때 0.9, 1.3, 2.3, 2.8 및 5.3 ppm 에서 유의미하게 증가하였다(p<0.01). 따라서 양성자 자기공명분광법은 고지방식 먹이를 먹고 2주 후부터 다양한 쥐의 간 지질 변화를 검출하고 특성화하는데 유용할 것이다.

Purpose – Korean women suffered from serious experience at unfair personnel practice in the work and coexistence between work and home affairs. The study examined woman workers' career plateau perception and success who worked for local public enterprises, that is to say, regional public corporation invested by local government, and investigated mediating effect of dual commitment as well as control effect of self-efficacy from point of view of various access to variables. Research design, data, and methodology – The study investigated women employees of regional public corporation by empirical analysis. The author collected 337 copies from 380 copies and made use of 325 copies after excluding copies with improper answer. SPSS 24.0 and AMOS 24.0 were used. Results – The study investigated effect of career plateau (structure and content) upon subjective career success (job satisfaction and career commitment) according to questionnaire material: First, not only structural plateau but also content career plateau had negative influence upon dual commitment (structure and career). Second, dual commitment had positive influence upon subjective career success from point of view of organizational commitment and career commitment. Third, structural career plateau had positive influence upon career success, and content career plateau had positive influence upon job satisfaction. Fourth, structural career plateau did not have significant influence upon job satisfaction, content career plateau and career satisfaction. Fifth, subordinate variables (organizational commitment and career commitment) had mediating influence upon career plateau cognition and subjective career success. Sixth, self efficacy did not control career plateau cognition and subjective career success. Conclusions – Women employee's career plateau cognition mediated dual commitment to have influence upon career success and to be likely to help career success theory study. Career plateau had negative influence upon dual commitment and career success of women workers. The study shall give base of counteraction from point of view of control and motivation of women workers thinking of career plateau.

본 연구는 고지방 식이로 유발된 내당능 장애 모델의 학습 및 기억력 장애에 대한 스피룰리나 섭취의 개선 효과를 확인하고자 하였다. 당부하 검사(IPGTT)를 통하여 고지방식이를 통한 내당능 장애가 유발되었음을 확인하였고, SP는 HFD 대비 약 20%의 당 내성 개선효과를 나타내는 것으로 나타났다. 고지방 식이로 인지 기능 손상을 유발시킨 동물모델의 Y-maze 및 Morris water maze 시험을 진행한 결과는 SP에서 CND 대비 학습 및 장·단기기억력 장애가 유의한 수준으로 개선됨을 확인하였다. 실험동물의 혈청분석 결과는 스피룰리나 섭취가 일반식이로 전환한 것과 비교하였을 때, HFD 대비 LDLC 감소 및 HTR(HDLC의 비율) 증가를 통한 혈중 콜레스테롤 개선에 효과를 나타냈다. 적출된 뇌와 간 조직을 대상으로 SOD 활성, oxidized GSH 함량 및 MDA 함량을 측정한 결과 스피룰리나 섭취가 뇌조직뿐만 아니라 간 조직에서 항산화 활성을 향상시켜 주는 것도 역시 확인할 수 있었다. 이러한 결과는 고지방 식이를 통한 혈당 상승 및 이로 인한 기억능력 저하에서 스피룰리나 섭취는 CND 수준으로의 개선 효과를 유도할 수 있음을 확인하였다. 또한, 스피룰리나 섭취는 뇌 기능과 직접적으로 연관된 콜린성 시스템 손상에도 CND 대비 AChE 활성에 더 높은 저해효과를 나타냄을 확인하였다. 따라서 본 연구는 스피룰리나가 고지방 식이로 유도된 내당능 장애 모델에서 발생될 수 있는 뇌기능 저하에서 혈중 콜레스테롤 개선 효과, 항산화 효과 및 AChE 저해효과를 통하여, 비만으로 유도될 수 있는 대사성 인지 장애에 대한 개선 소재로의 잠재적인 가능성을 갖는 것으로 판단된다.

본 연구는 클로렐라 건조분말을 산란계의 사료에 첨가해서 계란의 품질과 난황의 지방산 조성에 미치는 영향에 대해서 분석하였다. 시험에 사용한 유기 산란계 사료의 수분함량은 약 12.8%, 회분함량은 10.8%, 조단백질함량은 18.0%, 조지방 함량은 2.5%이었다. 사료에 첨가한 클로렐라분말의 수분함량은 약 1.54%, 회분함량은 6.53%, 조단백질은 54.56%, 조지방 함량은 2.45%이었다. 계란 난각의 색깔은 클로렐라를 급여한 후, 시간의 경과에 따라 대조구에 비해 유의적으로 진해졌다. 파각강도는 클로렐라를 급여한 후, 10일까지 증가하였으며 대조구에 비해 유의적으로 강하였다. 계란 난각의 두께도 클로렐라를 급여한 것이 대조구에 비해 유의적으로 두꺼웠다. 클로렐라 급여한 계란 난백의 높이는 대조구에 비해 높았다. 계란 난백의 품질 기준이 되는 호유닛은 클로렐라를 급여한지 10일 후, 92.0 HU로 대조구(84.8 HU)에 비해 유의적으로 높았다. 클로렐라를 급여한 계란 난황의 황색도도 대조구에 비해 유의적으로 진한 황색을 나타내었다. 클로렐라를 급여한 계란의 무게는 급여 15일 후, 대조구에 비해 7.5% 증가하였으며, 단백질함량은 급여 10일과 15일 후, 대조구에 비해 각각 11.9%, 10.7% 증가하였다. 클로렐라 급여에 따른 계란 노른자의 지방산 함량의 변화를 조사한 바, 난황의 주요 지방산 조성은 oleic acid, trans-linoleic acid, palmitic acid, α-linolenic acid, stearic acid, DHA, EPA, palmitoleic acid, heptadecanoic acid 순으로 나타났다. Palmitoleic acid는 클로렐라를 급여한 처리구가 대조구에 비해 감소하였다. 포화지방산은 클로렐라를 급여한 계란보다 대조구에서 높게 나타났고, 불포화지방산은 대조구보다 클로렐라를 급여한 계란에서 유의적으로 높게 나타났다. 불포화지방산과 포화지방산의 비율은 대조구보다 클로렐라를 급여한 계란이 높았다. 이상의 결과를 종합하여 볼 때 클로렐라 생균분말을 산란계의 사료에 첨가해서 급여할 경우 계란의 품질 향상과 난황의 불포화지방산 함량을 높이는데 긍정적인 영향을 미치는 것으로 판단된다.

This study was conducted to determine the effects of dietary bentonite supplementation on the fatty acid profiles of eggs in older laying hens. A total of 90 Hy-line Brown layers, 74 weeks of age, were confined in 6 wire cages and then assigned randomly to two groups to receive one of the two diets (3 replicates of 15 older layers each) containing 0 and 0.2% bentonite for 4 weeks. After the 4-week feeding trial, no remarkable differences in individual fatty acid, saturated fatty acid (SFA), unsaturated fatty acid (UFA), and mono unsaturated fatty acid (MUFA) levels were found. However, linoleic acid, docosahexaenoic acid, and poly unsaturated fatty acid (PUFA) and UFA/SFA ratio were influenced by 0.2% bentonite. In conclusion, supplementation with 0.2% bentonite improved the profiles of PUFA more than those of SFA, UFA, and MUFA inthe eggs of the older laying hens.

Background : Agaricus bisporus mushroom, which originated from Europe, is an edible mushroom with a unique taste and flavor and is the most widely cultivated mushroom type worldwide, particularly because of the relatively high protein, dietary fiber, and vitamin D contents of the fruit body, which have human health benefits like anticancer and DNA damage protection. However, to our knowledge, few studies have been conducted on the ability to discriminate mushroom’s bioactive compounds according to regional production sites. Thus, this study was aimed to examine the regional difference of selected bioactive compounds in of Agaricus bisporus mushroom. Methods and Results : The cultivars (cv.) “Saedo” and “Saehan” obtained from five regions of Korea were measured their phenolics and fatty acids profiling by LC/MS and GC-FID system, respectively. Among 54 targeted phenolics, protocatechuic, p-hydroxybenzoic, caffeic, 2,4-dihydrobenzoic, p-coumaric, and salicylic acids were found in the cv. Saedo and cv. Saehan fruit body. Also, tyrosine and phenylalanine, which are precursor metabolites of phemolics, were also observed in the A. bisporus fruit body. In particular, phenylalanine and p-hydroxybenzoic acid were the most abundant phenolic precusor and metabolite present in the A. bisporus, and was significant with the production regions (p < 0.05). The FA content was significant with the production region, but not much differed (p < 0.05). The n-6 FA including linoleic acid was the major FA in the mushroom, accounting > 70% over total FA content. However, the n-3 FA was the n-6 FA was very minor FA metabolites. Conclusion : These preliminary results can extend knowledge of regional difference of certain targeted bioactive compounds in A. bisporus mushroom produced in Korea, contributing to reliable regional difference with potential broader applications for the international mushroom market.

Background : Perilla (Perilla frutescens) seed oil is known to contain high omega-3 fatty acid than other plant oils. This study was aimed to investigate the fatty acid composition in seeds of 255 perilla accessions from South Korea and recommend the potential genetic resources rel ated to their fatty acid composition. Method and Results : Total oil was extracted by soxhlet extraction apparatus and the fatty acid compositions were analyzed by gas chromatography (GCMS QP2010 ULTRA, SHIMADZU, JP). Total oil contents ranged from 22.41 and 47.62% with an average content of 34.20%. Palmitic, stearic, oleic, linoleic, and linolenic acid contributed 4.46 to 7.67%, 1.64 to 4.11%, 9.15 to 26.39%, 11.89 to 28.76%, and 50.24 to 64.16% to the total oil content, respectively. Seeds from Jeollabuk-do showed the highest average total oil content (41.14%) compared to other regions (p < 0.05), while the linolenic acid composition was the highest in the samples from Chungcheongnam-do (59.48%) (p < 0.05). The cluster analysis segregated the perilla seed accessions into two major clusters. ANOVA revealed that there was a significant difference between group Ⅰ and group Ⅱ (p < 0.05). Group Ⅰ (86 accessions) characterized as higher palmitic, stearic, oleic, and linolenic acid compositions than group Ⅱ (169 accessions). The highest content of linolenic acid were recorded in accessions K126190, K135903, and IT283646 from group Ⅰ, and accessions IT108680, IT208894, and IT111050 from group Ⅱ had high total oil content. Linoleic acid content showed a strong negative correlation with palmitic acid (r = -0.726*) and oleic acid (r = -0.678*) content. Principal component analysis (PCA) revealed that the first two principal components together explained 68.75% total variation. Conclusion : Our results showed that accessions K126190, K135903, IT283646 which exhibited high linolenic acid composition and accessions IT108680, IT208894, IT111050 which exhibited high total oil composition, could be useful to develop new functional oil materials.

Background : Obesity, a global health problem and a chronic diseases, is associated with increased risk of developing type 2 diabetes and coronary heart diseases. A wide variety of natural remedies have been explored for their obesity treatment potential. To elucidate the anti-obesity effect of ginsenoside Rg5 : Rk1 (Rg5 : Rk1), a mixture of protopanaxadiol type ginsenosides isolated from Panax ginseng Meyer in a 3T3-L1 adipocytes. Methods and Results : In order to determinate the anti-obesity effect of Rg5 : Rk1, Oil Red O staining and triglyceride (TG) content was assessed. Furthermore, to elucidate the possible mechanism whether Rg5:Rk1 affects lipid accumulation, mRNA and protein expression analyses of adipocyte markers such as STAT3, PPARγ, CBEPα and ap2 were carried out. Rg5:Rk1 treatment showed an inhibition of lipid droplet accumulation and decrease on TG content. In addition, expression of STAT3, PPARγ, CEBPα and ap2 were decreased in dose dependent manner. Similar to these results, Rg5:Rk1 treatment reduced PPARγ and CEBPα protein expression. Conclusion : Rg5 : Rk1 treatment exhibits anti-adipogenic activity by down-regulation of the STAT3PPARγ/CEBPα pathway in 3T3-L1 adipocyte cell line.

Background : Recently demand of Rehmannia glutinosa Liboschitz increased rapidly using a food and cosmetics as an emphasis on well-being. Cultivation tests of Rehmannia glutinosa for new varieties are tested around Geumsan area of Chungnam province. It is different for the soil condition and wether between Chungnam area and Gyeongbuk area. It is needed cultivating tests of new variety region of north Gyeongbuk. Methods and Results : Test variety of Rehmannia glutinosa was Dagang and test place was 918 Banguri Jangsu Yeongju. Planting density of Dagang was 20 × 20 ㎝, 30 × 20 ㎝, 40 × 20 ㎝ and all seedstocks were disinfected before sowing. Composite fertilizer use 60 ㎏/10 a and press cake use 600 ㎏/10 a on the test field. Black vinyl muching was using for preventing of grass. Leaf length, leaf width and leaf number were no difference in growth among 20 × 20 ㎝, 30 × 20 ㎝ and 40 × 20 ㎝. There was no difference in root length, root number root weight between planting distances, and root width was the largest at 30 × 20 ㎝ and 16.3 ㎜, Followed by 20 × 20 ㎝ and 40 × 20 ㎝ in order. Total root weight of 20 × 20 ㎝ was higher than other plant density and total income of 20 × 20 ㎝ was higer than others. Roots rot of root was not significant difference among plant density. It was the first test Dagang around north Gyeongbuk area and plant density of 20 × 20 ㎝ was higher output than 30 × 20 ㎝, 40 × 20 ㎝. Conclusion : Plant density of 20 × 20 ㎝ was selected for cultivating Dagng around north Gyeongbuk area. 20 × 20 ㎝ of cultivating Dagang get a more roots and also increase famers income.