성능보장설계는 교각이 완전한 소성회전성능을 발휘할 때까지 다른 구조요소들과 교각 자체가 취성파괴 되지 않도록 설계하여 교량 전체 시스템의 연성파괴를 보장하기 위한 것으로서, 현행 도로교설계기준에는 명시적으로 규정되어 있지 않으나 대부분의 외국 교량내진설계기준에 채택되어 있다. 성능보장설계에서는 철근콘크리트 교각의 휨 초과강도를 구하고 이를 변환한 전단력을 교각, 기초, 말뚝에 작용하는 횡하중 설계전단력으로 결정하여 교각의 전단설계, 기초설계, 말뚝설계를 수행하도록 규정한다. 이 때 교각의 최대 소성모멘트를 결정하는 방법은 설계기준별로 각기 다른데, 이는 각 국의 재료 시공환경이 다르기 때문이다. 본 연구에서는 국내에서 사용하는 철근의 인장강도 측정치 3,407개와 콘크리트 압축강도 측정치 5,405개의 분석을 통하여 재료 초과강도계수를 제안하였고, 이를 적용하여 휨 초과강도를 결정하는 방법을 제시하였으며, 1,500개의 교각단면에 대한 모멘트-곡률 해석을 수행한 후 통계분석을 통하여 우리나라 실정에 적합한 초과강도계수를 제안하였다.

형상비가 상대적으로 작은 철근콘크리트 교각에 지진작용으로 인한 반복 횡하중이 작용하면 초기단계와 중간단계의 변위에서는 휨 거동을 보이다가 최종변위단계에서는 전단에 의해 파괴되는 휨-전단 거동을 보인다. 휨-전단 파괴거동을 보이는 교각은 휨 파괴거동을 보이는 교각에 비하여 연성능력이 저하되므로, 내진설계 또는 내진성능평가에서 극한변위를 해석적으로 결정하기 위해서는 휨성능곡선과 함께 전단성능곡선 모델을 적용하여야 한다. 본 논문에서는 원형교각에 대한 기존 모델을 수정한 전단성능곡선 모델을 제안하였고, CALTRANS 모델, Aschheim등의 모델, Priestley 등의 모델, 제안모델의 특징을 비교하였다. 또 국내에서 수행된 실물크기 기둥 실험체를 대상으로 전단성능곡선 모델을 평가하였다. 제한된 범위의 소수 실험결과에 대한 적용으로서 일반화하기는 어려울 것이지만, 실험결과와 비교 검토한 결과 제안모델이 파괴형태의 예측과 변위성능 예측의 정확도에서 매우 우수한 것으로 평가되었다.

길이 40m,폭 5.5m의 단일피복 구조의 8연동 무가온하우스 상단부에 설계적설심 19.1 cm의 눈이 쌓인다는 조건과 시설 측면으로 설계풍속 36.6 m·s-1의 바람이 분다는 조건 그리고 참고자료로 활용하기 위해 적용한 최대적설심 37.8cm의 눈이 쌓인다는 조건과 순간최대풍속 60.0 m·s-1의 강풍이 분다는 조건에서 유동 및 구조강도 해석을 수행하였다. 적설하중 조건에서는 설계적설심 19.1 cm와 최대적설심 37.8cm에서 파이프에 걸리는 최대응력이 각각 53.8 N·mm-2과 107 N·mm-2으로 재료의 허용응력 보다 작은 것으로 나타나 안전한 것으로 분석되었으나, 설계풍속 36.6 m·s-1와 순간최대풍속 60.0 m·s-1의 풍하중 조건에서는 파이프에 걸리는 최대응력이 각각 250 N·mm-2과 672 N·mm-2으로 재료의 허용응력을 모두 초과하여 플라스틱하우스가 불안전한 것으로 분석되었다.

Studies to evaluate distribution of markers in normal keratinocyte and their immortalized keratinocyte are appropriate to evaluate the normal and preneoplastic lesion of oral cancers as biochemical and cytochemical changes associate with tumorigenesis being not completely understood. Complementary DNA microarray containing 6000 sequence -verified cDNA elements was used to systematically characterize the variation in gene expression patterns of NHOK cells vs. immortalized keratinocyte by HPV16 E6-E7(IHOK). Examination of gene expression that is 85 clones cDNAs exhibits greater than 2 fold overexpression in NHOK probes relative to IHOK probe, 147 cDNAs reveal greater 2 fold overexpression in IHOK relative to NHOK probe.The high similarity in gene expression (96.5%) between IHOK and NHOK cells suggests that only an additional 232/6720 (3.5%) of the genome is differentially gene activated during HPV16 immoratlized keratinocyte growth and differentiation. Examination of gene expression that differs between NHOK and IHOK cellsapprear to be related to : cell adhesion & recognition, cell cycle regulator, apoptosis, transciption factors, growth factors and therir receptors, cytoskeletal and extracellular matrix proteins, signal transduction modulators and effectors, and miscellaneous. The gene expression of cell recognition factor such as endothelin 1, collagen IV, fibronectin, and SPR1 in IHOK were upregulated. Distinct or duplicated cDNA clones representing the same gene were typically clustered in adjacent rows in the clustered gene map. Therefore the differentially expressed and identified genes should be informative in studying oral epithelial cell carcinogenesis and such studies should foster the research of molecular markers allowing to assess the phenotypeof malignant epithelial tumor.

Nitric oxide (NO) has been known to inf1uence cell fate through apoptotic or necrotic cell death. Here, we investigated the role of nitric oxide on the growth and viability of immortalized human salivary gland (HSG) cells 띠 vitro. Treatrnent of HSG with a NO donor, S-nitroso-N-acetyl-DL-penκi1lamine (SNAP), significantly diminished the growth rate of HSG in a concentration dependent manner. However, this retardation of cell비ar growth rate was not corresponded to the apoptotic cell death of HSG cells, because there were no characteristic apopto디c features such as condensation of nuclear chromatin, nuclear fragmentation, and the apoptotic peak of propidium iodide (PI)-stained nuclei by flow cytome띠. 까ùs implies that HSG cells are resistant to NO-mediated 다π。to:잉city. 1n SNAP treated HSG cells, cell cycle analysis revealed that the number of G2/M phase increased markedly, according to while the percentage of cells in GO/Gl and S phases was not significantly affected. Otherwise, high concentrations of SNAP increased both P1 and annexin V positive cells. 1nterestingly, preincubation of HSG cells with iron chelator, deferoxamine (DFO), significantly diminished NO cytotoxicity more than when HSG cells are only incubated with SNAP which su잃.ests the role of iron homeostasis in NO-mediated cell death of HSG cells. 1n addi디。n ， treatrnent of HSG cells with SNAP specifically cleaved iron regulatory protein-2 (IRP2) while not affecting 1RP1. Collectively, the mπent results s멍gest that NO has a potential to control HSG cell growth through cell cycle arresting at G2/M phase. 1n addi디on ， intracellular iron homeostasis nùght play an important role in regulating cell survival of HSG cells

Epithelial-mesenchymal interaction is well known to have an importance during the organ development as well as cell growth and differentiation. However, in vitro experimental model is not well developed to reproduce in vivo cellular micro-environment which provide a epithelial-mesenchymal interaction. The aims of this study were to develop and evaluate the in vitro experimental model that maintains epithelial-mesenchymal interaction by organotypic raft culture, and to characterize biologic properties of three-dimensionally reconstituted human normal oral kertinocyte(NHOK) and immortalized human oral keratinocytes(IHOK) by histological and immunohistochemical analysis. The results were as follows; 1. Best condition of three dimensionally reconstituted IHOK & HaCaT cells are 14 days air-exposure cultivation, 3 days of submerged state, and dermal equivalent consisting type I collagen and IGF cells. 2. In comparison to IHOK, there was better preservation of the overall epidermal strucutures in oral cracioma cells (HN30) & HaCaT cells by organotypic cultures. But orgnaotypic co-culture of the normal keratinocyte showed the thinnest epithelial layer formation. 3. PCNA was detected primarily in the basal layer of normal mucosa and NHOK, whereas was shown throught the epithellium except surface layer of IHOK cells, and it's expression was similar to that of CIS of biopsied patient's tissue 4. Involucrin is expressed in the upper layer of oral mucosa and NHOK raft, but staining for involucrin was induced in the IHOK rafts indicating differentiation is incomplete, and the staining pattern in the IHOK raft was not uniform. 5. Normal oral keratinocyte raft showed weak immunostaining for p53, and p53 expression of IHOK raft increased rahter than in NHOK. In organotypic cultures of normal cells and IHOK, p53 expression was restricted to the proliferative part of epithelium. This is consistent with expression pattern in biopsy specimens of the normal and CIS tissue. 6. In artifically reconstructed NHOK, the pattern of keratin staining showed both similarity and differences from that of intact normal mucosa. An obvious difference was increased expression of CK10 & CK19, and decreased expression of CK6 in a reconstructed NHOK rather than in normal mucosa, and similar expression was in CK4 and CK16. 7. CK19 & CK16 were strongly positive in HPV immortlaized keratinocyte rafts rahter than in NHOK, an indicator of premalignant or malignant changes, while CK10 & CK6 were decreased, and organotypic cultures of IHOK express was similar keratin expression as epithelial dysplasia or CIS tissue. These results suggest that three-dimensional organotypic co-culture of normal oral & immortalized keratinocytes with dermal equivalent consisting type I collagen and fibroblasts results in similar morphologic and immunohistochemical characteristics to in vivo patient specimens. Thus this organotypic system can be used for studing mechanism of epitehlial-mesenchymal interaction in particular regulating epidermal diffenentiation and morphogenes