The overall process, from the pre-treatment of aluminum substrates to the eco-friendly neutral electroless Ni-P plating process, was observed, compared, and analysed. To remove the surface oxide layer on the aluminum substrate and aid Ni-P plating, a zincation process was carried out. After the second zincation treatment, it was confirmed that a mostly uniform Zn layer was formed and the surface oxide of aluminum was also removed. The Ni-P electroless plating films were formed on the secondary zincated aluminum substrate using electroless plating solutions of pH 4.5 and neutral pH 7.0, respectively, while changing the plating bath temperature. When a neutral pH7.0 electroless solution was used, the Ni-P plating layer was uniformly formed even at the plating bath temperature of 50 oC, and the plating speed was remarkably increased as the bath temperature was increased. On the other hand, when a pH 4.5 Ni-P electroless solution was used, a Ni-P plating film was not formed at a plating bath temperature of 50 oC, and the plating speed was very slow compared to pH 7.0, although plating speed increased with increasing bath temperature. In the P contents, the P concentration of the neutral pH 7.0 Ni-P electroless plating layer was reduced by ~ 42.3 % compared to pH 4.5. Structurally, all of the Ni-P electroless plating layers formed in the pH 4.5 solution and the neutral (pH 7.0) solution had an amorphous crystal structure, as a Ni-P compound, regardless of the plating bath temperature.

A strain of Alexandrium species was established by isolating cells from Jangmok Bay, Korea. Its morphology and molecular phylogeny based on LSU rRNA gene sequences were examined. In addition, growth responses of this Alexandrium species to changes in temperature, salinity, and nutrient concentrations were investigated. This Alexandrium species from Jangmok Bay had a ventral pore on the 1′, which was morphologically consistent with previously described Alexandrium tamarense and A. catenella. Phylogenetic analyses revealed that this isolate was assigned to A. pacificum (Group IV) within A. tamarense species complex. In growth experiments, relatively high growth rates and cell densities of A. pacificum (Group IV) were observed at 15°C and 20°C. This species also grew under a wide range of salinity. This indicates that this Korean isolate of A. pacificum (Group IV) is a stenothermic and euryhaline species. In growth responses to changes in nutrient levels, enhanced growth rates and cell densities of A. pacificum (Group IV) were observed with additions of nitrate and phosphate. In particular, rapid uptakes of phosphate by A. pacificum (Group IV) were observed in experimental treatments, indicating that the increase in phosphate concentration could stimulate the growth of A. pacificum (Group IV).

Anthropogenic disturbances on freshwater ecosystem are known to degrade biodiversity, especially on fish assemblage. In this study, we have conducted fish surveys to identify impact of a bridge construction on fish assemblages. A total of eight study sites were surveyed in the Danjang and the Dong Stream in southern part of South Korea from June to November in 2021. The fish samplings were carried out five times, using cast-nets (10×10 mm mesh size), scoop-nets (4×4 mm, 5×5 mm mesh size), set-nets (10×10 mm mesh size), and fish traps (3×3 mm mesh size), along with the Stream/River Ecosystem Survey and Health Assessment by the Ministry of Environment of Korea and basic water quality measurement. Also, we applied the species diversity index and length-weight relationship regressions on certain species to identify interspecific growth rate differences in accordance with study sites. As a result, a total of 782 individuals, 23 species and 10 families were collected. The dominant species was Zacco Koreanus and relative abundance was 50.89%. When applying the length-weight relationship regressions on certain species, the ‘b’ value for Z. Koreanus was lower at the downstream points than at the upstream points of the construction site. In addition, when comparing to the results of the past survey, relative density of demersal fish at the upstream and downstream points decreased from 26% to 1.4%, and from 18% to 6.3%, respectively. In conclusion, it is considered that bridge construction negatively affects the habitat of fishes, especially on demersal fishes. Therefore, appropriate conservation efforts such as installation of silt protector and sand sedimentation pond are needed to alleviate the disturbance in habitat such as occurrence of turbidity and destruction of micro-habitats.

Despite the long history of mushroom use, studies examining the genetic function of mushrooms and the development of new varieties via bio-molecular methods are significantly lacking compared to those examining other organisms. However, owing to recent developments, attempts have been made to use a novel gene-editing technique involving CRISPR/Cas9 technology and genetic scissors in mushroom studies. In particular, research is actively being conducted to utilize ribonucleoprotein particles (RNPs) that can be genetically edited with high efficiency without foreign gene insertion for ease of selection. However, RNPs are too large for Cas9 protein to pass through the cell membrane of the protoplasmic reticulum. Furthermore, guide RNA is unstable and can be easily decomposed, which remarkably affects gene editing efficiency. In this study, nanoparticles were used to mitigate the shortcomings of RNP-based gene editing techniques and to obtain transformants stably. We used Lentinula edodes (shiitake mushroom) Sanjo705-13 monokaryon strain, which has been successfully used in previous genome editing experiments. To identify a suitable osmotic buffer for the isolation of protoplast, 0.6 M and 1.2 M sucrose, mannitol, sorbitol, and KCl were treated, respectively. In addition, with various nanoparticle-forming materials, experiments were conducted to confirm genome editing efficiency via the formation of nanoparticles with calcium phosphate (CaP), which can be bound to Cas9 protein without any additional amino acid modification. RNPs/NP complex was successfully formed and protected nuclease activity with nucleotide sequence specificity.

Mushroom production in Korea is concentrated on five major mushroom types. To create a new source of income for farmers, it is necessary to establish new mushroom production and marketing systems. This study was conducted to evaluate the marketability of and establish a marketing strategy for Pleurotus nebrodensis variety 'Uram'. The evaluation of distributors showed that it was necessary to cultivate mushrooms of uniform shape and size, to compensate for their low storability, and to sell them in small packages. The consumer evaluation showed that the texture of P. nebrodensis had the highest level of satisfaction for quality, but the levels of satisfaction for size and shape were low. In the consumer evaluation, as in the distributor evaluation, improvements in cultivation were found to be necessary, as the storage time is short due to a high moisture content. An evaluation of the market gave the following results regarding the marketing strategy. It was found to be necessary to consider the production of mushrooms of a uniform shape and size and sell them in small packages in the range of 150 to 300 g. The price of the mushrooms should be set using a high-end strategy for high-end sales. The mushrooms should be introduced to local food and eco-friendly stores in the early stages of production. Subsequently, if farmhouse production increases, shipments should be made to wholesale markets through a regular contract. Finally, considering that P. nebrodensis is an unfamiliar mushroom to consumers, it is necessary to promote it by increasing the accessibility of consumers through tasting events and experience groups.

This study measured the suspended fungal concentration in indoor multiple facilities nationwide. The regions were selected as representative cities by region: Seoul, Gyeonggi (Incheon), Gangwon, Gwangju, Daejeon, and Busan. A total of 2028 regional comparisons, including department stores, schools, public toilets, libraries, and banks, subway, sports facilities and comparative analysis were conducted for each multi-use facility industry. Among the nationwide, Among the regions, the average concentration of floating mold in indoor multi-use facilities was the lowest in Busan at 394.67 CFU/m3, followed by Gyeonggi and Incheon 487.90 CFU/m3, Seoul 542.84 CFU/m3, Daejeon 809.30 CFU/m3, Gangwon 1,145.22 CFU/m3, Gwangju was 1,371.10 CFU/m3 in the order. Busan was the lowest, and Gangwon was the highest. The reason that Busan, which has a high average temperature and population density, shows a lower mold concentration than Gangwon, is that floating mold in the indoor air is not affected by the external atmospheric environment, population density, and number of facility users. Although it cannot be said that there is no influence of the atmospheric environment, it was found that the indoor environment has different characteristics from the outdoor environment. The importance of air quality management has been confirmed, and further, it is necessary to subdivide the management standards by region and multi-use facilities, and the management standards need to be converted to maintenance rather than recommendations.

This study was conducted to investigate the optimum supplementation ratio when replacing cotton seed meal with rapeseed meal for cultivating oyster mushrooms. The chemical properties among the treatments were pH 4.5~4.7, total carbon content was 46.3~46.5%, total nitrogen content was 1.6~1.7%, and carbon-nitrogen ratio was 27.0~27.8. These did not differ significantly from the control. Mycelial growth was 85.1 mm (Heuktari), and 72.8 mm (Suhan-1Ho) in medium containing 5% rapeseed meal, with no significant difference in mycelial density between cultivars. Fruiting body characteristics are as follows: In 'Heuktari', the color of the pileus was darker than control with an L value of 32.5, and pileus thickness and diameter were 3.2 mm and 27.6 mm, respectively. Stipe length and diameter were 86.0 mm and 9.1 mm, respectively. Valid stipe number per bottle (1,100 cc) was 25.9, and a yield of 189.7 g/1,100 cc was recorded in mixed medium containing 5% rapeseed meal (higher than control). In mixed medium containing 10% rapeseed meal, the yield was 184.5 g/1,100 cc (comparable to control). In 'Suhan-1Ho', supplemented with 5% rapeseed meal, pileus color was darker than control color, with an L value of 28.8. Pileus thickness was 4.5 mm. Stipe diameter was 12.3 mm (higher than control), and yield was 145.5 g/1,100 cc (comparable to control).

The purpose of this study was to breed a variety of stable productivity and high storage characteristics of white Hypsizygus marmoreus, which has high demand at domestic and global market due to a low bitter taste and the preference for white species. Accordingly, ‘Baekmaru’ was bred by hybridizing brown and white cap color species and backcrossing with white species. The ‘Baekmaru’ variety was bred by crossbreeding the brown and white species and backcrossing the white species. Through repeated cultivation of ‘Baekmaru’, a variety with a low contamination rate was selected when culturing the spawn for stable cultivation. As a results of demonstration test, the yield of ‘Baekmaru’ was 14% higher than that of the commercial variety. In addition, among the fruiting body characteristics of ‘Baekmaru’, diameter and thickness of the pileus were 16.43±15.27mm and 6.46±0.58mm, which were slightly higher than the commercial variety, and the hardness was 2.69±0.89N for the pileus and 3.09±0.89N for the stipe. The shelf life showed less change in thickness and color of pileus compared to commercial variety. The hardness of pileus of 'Baekmaru' was maintained in the range of 3.5 to 4.0N in the 4oC and 4oC and 20oC mixed treatment until 30 days of storage, and was higher than that of the commercial variety. Therefore, it was suggested to be excellent in storability.

To optimize initial cooling conditions, forced-air cooling was applied to freshly harvested oak mushrooms at 2 levels (0oC for 30 minutes, at 0oC for 1 hour) followed by room cooling at 3 levels (-3oC for 1 day, 0oC for 1 day, 3oC for 1 day). After initial cooling, the oak mushrooms were packaged with PVC film, then held in a storage room at 1oC for 6 weeks. Quality characteristics and percentage marketability were then investigated. As a control, Mushrooms were placed in storage with no initial cooling. The quality factors impacting marketability of fresh oak mushrooms were color change and appearance of decay. Off-odor did not occur or developed only slightly, so it did not affect oak mushroom quality within 6 weeks of low temperature storage. In all treatment groups, the shelf life in which 100% marketability was maintained was up to 3 weeks. At week 5, percent marketability of the 3 treatment groups 1 hour room cooling treatment at 0°C, 1 hour forced air cooling, and control was 100%. 80% In the group that underwent 30 min forced air cooling retained 80% marketability, and the group exposed to 1 day in room cooling at -3oC retained 86.7% marketability. At week 6 of 1oC storage, the marketability ratio was 80% in the 1 day room cooling at 0oC group, 66.7% in the 1 day room cooling at 3oC group, 46.7% in the 1 hour forced air cooling group, and 33% or less in all other treatment groups. Therefore, the most suitable initial cooling parameter to extend shelf-life of oak mushrooms is 1 day of in room cooling at 0oC immediately after harvest.

As the importance of public health increases with the spread of infectious diseases, functionality has become a factor affecting consumers’ purchase of mushrooms. Therefore, the bioactive components of button mushrooms (Agaricus bisporus), which are generally known to promote button mushroom consumption, were analyzed. White and brown button mushrooms were compared and white beech mushroom (Hypsizygus marmoreus) were used as a control. White button mushrooms had higher sugar and inorganic potassium concentrations than brown button mushrooms, whereas sodium, magnesium, and vitamin C concentrations were not significantly different between the different button mushrooms. Moreover, there was approximately twice as much ergosterol in white button mushrooms than brown button mushrooms. Brown button mushrooms had higher concentrations of β-glucan and oxalic acid than white button mushrooms, but there was no significant difference in total organic acid content between the two mushroom types. High concentrations of the essential amino acids, ergothioneine, isoleucine, and leucine and the non-essential amino acids, glycine and alanine, were observed. Concentrations of the vitamin B group and total polyphenols were also high.

반추위 미생물체 단백질의 아미노산 조성이 한우의 에 너지원 사료에 따라 차이가 있는지를 조사하고자 본 연구 가 수행되었다. 본 실험은 국내 한우 비육우 사육에 주로 이용되는 에너지원 사료인 옥수수(T1), 생미강(T2), 소맥 (T3), 소맥피(T4)를 이용하여 in vitro 반추위 발효실험을 진행하여 발효 24시간 후 배양액의 발효 성상, 미생물체 단백질 합성량(MPS), 미생물의 아미노산 조성 및 미생물 균총 변화를 분석하였다. 배양액의 pH는 T4가 6.02로 다 른 처리구들에 비해서 가장 높았으며, T1은 4.84로 가장 낮았다(p<0.05). Total VFA 농도는 T2가 96.80mM로 다른 처리구들에 비해서 가장 높았고, T1이 53.35mM로 가장 낮았다(p<0.05). 미생물체 단백질 합성량은 T3가 222.50g/L로 다른 처리구들에 비해서 높았으며(p<0.05), T1과 T4는 유의적인 차이는 없었지만 T1이 가장 낮았다. DGGE band 분석결과 모든 처리구는 57.5% 이하의 유사도를 나타내었으며, 급여되는 사료에 따라서 미생물 군집 이 다르게 나타났다. 미생물 내 총 아미노산 함량은 T3와 T4가 다른 처리구보다 높았다(p<0.05). 미생물의 아미노산 조성 중 대부분의 필수 아미노산은 T4가 다른 처리구들에 비해서 낮았다. 하지만 methionine은 다른 필수 아미노산 과 다르게 T4에서 가장 높았다(p<0.05). 곡류 사료 위주의 급여 시 결핍되기 쉬운 lysine은 모든 처리구에서 미생물 의 아미노산 조성이 8.05~8.98%의 범위에 있었다. 따라서, 본 연구에서 반추 가축 급여 에너지 사료원에 따른 반추 위 내 미생물 발효특성과 미생물 군집의 차이가 미생물의 아미노산 조성을 변화시킨다는 것을 확인하였다.

목적 : 본 연구는 문화적, 사회적, 환경적 특성이 반영된 초등학생들의 작업수행활동 항목을 도출하여 아동 평가 및 부모 상담 시 유용하게 사용할 수 있도록 작업수행활동 평가도구를 개발하기 위한 기초를 제공 하고자 시행하였다. 연구방법 : 문헌 조사를 통해 수집된 항목 분류 및 평가도구의 항목 중 총 645개의 활동 항목을 수집하여 최종 46개의 항목을 선정하였다. 이후 작업치료실행체계-Ⅲ(Occupational Therapy Practice Framework- Ⅲ; OTPF-Ⅲ의 작업 범주와 생활시간조사(2014)의 행동분류를 기준으로 기초 항목 도출 과정을 거쳐 작업치료사 16명, 초등학교 교사 16명, 작업치료학과 교수 8명, 총 40명의 전문가를 대상으로 3차례의 델파이 조사를 시행하여 최종 항목을 선정하였다. 조사도구로는 리커트 척도인 구조화된 질문과 개방형 질문이 혼합된 설문지를 사용하였다. 결과 : 델파이 조사를 통해 수거된 설문지의 응답을 토대로 항목의 적합성을 항목별 중요도, 안정성, 내용 타당도(Content Validity Ratio)로 확인하였고 개방형 질문에 대한 의견을 수렴하여 항목의 삭제, 수정 및 추가 여부를 결정하였다. 이후 OTPF-Ⅲ 작업 범주의 4개 영역, 생활시간조사 행동분류의 7개 영역 에 따라 작업수행활동 29개의 항목을 추출하고 분류하였다. 결론 : 본 연구를 통해 문화적, 사회적, 환경적 배경이 반영된 국내 초등학생의 작업 수행 활동은 29개 항 목으로 최종 도출되었다. 본 연구결과를 바탕으로 향후 임상 작업치료사들이 의미 있는 작업 목표 선정 을 위한 활동평가 도구개발에 활용하고 부모 면담 시 간편하고 유용하게 사용하기를 기대한다.

최근 비풀러렌 전자수용체 소재 개발로 태양전지 및 광검출기 등 유기광다이오드 분야의 상당한 진보를 나타내고 있 다. 비풀러렌 소재의 자유로운 구조 개질 가능성을 바탕으로 흡광대역 자유 제어가 가능한 장점으로, 기존 태양전지 에서 구현이 힘들었던 고성능 반투명 태양전지, 실내 저조도 태양전지, 파장선택적 광검출기 등 다양한 응용을 가능 하게 한다. 본 리뷰에서는 유기태양전지를 비롯한 유기광다이오드의 광활성층에 활용되는 유기반도체 소재의 최신 연 구동향에 대해 다루고자 한다.

목적 : 본 연구는 요양시설에 거주하는 65세 이상 노인들을 대상으로 한국형 활동분류카드(Korean Activity Card Sort; K-ACS)를 통해 참여활동수준을 측정하고, 삶의 질에 미치는 요인에 대해 알아보 고자 하였다. 연구방법 : 2021년 7월 1일부터 2021년 8월 31일까지 수도권 요양시설 거주 노인 78명을 대상으로 자 가 보고식 설문지와 평가를 실시하였다. 대상자들의 참여활동수준, 우울, 자기효능감, 일상생활수행능력, 삶의 질과의 관계를 피어슨 상관분석과 다중회귀분석으로 알아보았다. 결과 : 요양시설 거주 노인을 대상으로 참여활동수준을 측정한 결과 전체 참여활동수준 27.8%, 수단적 일 상생활활동수준 26.9%, 여가활동수준 36.5%, 사회활동수준이 21.0%로 나타났다. 전체 참여활동수준은 수단적 일상생활활동수준(r = .937, p < .01), 사회활동수준(r = .858, p < .01)과 높은 상관성을 보 였다. 또한 삶의 질에는 정신적 요소인 우울(β = -.514, p < .001)과 자기효능감(β = .266, p < .05) 이 영향을 미치는 것으로 확인되었다. 결론 : 작업치료사는 노인들의 성공적인 노화를 돕기 위해 시설 내에 다양한 활동프로그램을 마련하고 노 인들이 활동에 참여할 기회를 제공하여야 한다. 이러한 작업치료사의 역할은 시설 거주 노인의 우울감을 완화하고 자기효능감, 참여활동수준을 높임으로써 궁극적으로 삶의 질을 향상시킬 것으로 기대된다.