Calcium is widely used for calcium supplement, additives. In the case of oyster shell which is intaked for calcium supplement have caused serious problems including environmental pollution because almost of it is thrown out so that is demanded expanding of using. However, there is no examples that develop a high value product available for food industry from oyster shell. In advanced research, It is confirmed that fired oyster shell has Anti-microbial effect and therefore, this study apply the anti-microbial effect of fired oyster shell to rice cake. Control is made by rice flour 300g, water 150mL. Experimental group are made by rice flour 300g, water150mL and Sample A (fired oyster shell), Sample B (fibrous calcium carbonate), Sample C (calcium + citric acid) and Sample D (coral calcium) 0.5wt% respectively. Texture properties are measured by texture analyzer (TA-XT, MHK corp. Korea) 5kg load cell. Rice cake added sample A, B, C had anti-microbial effect and rice cake added sample D had not anti-microbial effect.

1. 농촌개발협력 사업을 대상으로 SDSN의 공개작업반이 목표별로 제안하고 있는 SDGs지표와 선진 공여기관 및 국제기구(DFID, FAO, WB, 한국국제협력단 등)들이 사용하고 있는 기존 지표의 연계가능성을 검토하기 위해 동 분야 국내 전문가들을 25명을 대상으로 설문을 실시한 후 결과를 제시하였음. 2. ‘농업·농촌분야 SDG 지표 활용을 통한 성과 측정 방안’으로 중남미 농업·농촌 분야 개발협력 사업별에 대한 SDGs 기여 정도 측정안을 제시하였고, SDGs 성과지표 활용방안 제시를 위해 최근 KOICA가 추진한 중남미 농업·농촌분야 개발협력 사업 중 볼리비아 종합개발사업을 실례로 선정하여 분석하였음. 3. 농촌개발협력사업의 성과관리를 위해 SDGs 성과지표를 선정하고 SDGs 목표에 대한 기여 정도를 정량화하여 시범적으로 적용하는 방안을 제시함으로써 SDGs 목표별 기여 정도를 측정한다면 향후 우리나라의 개발협력 사업이 국제사회에 기여하는 정도를 정량화할 수 있고 , 이러한 방식의 도입은 보다 효과적인 개발협력 사업 추진을 위한 자료 및 정보를 제공하는 측면에서 의미가 있음.

The aim of the study was to investigate the effects of incentive spirometry and Ujjayi breathing technique on the pulmonary function of smokers. Subjects were individuals who had a smoking habit of at least a year. Subjects were randomly divided into 3 groups: the incentive spirometry group (n=8), Ujjayi breathing technique (n=9), and a group applying both incentive spirometry as well as Ujjayi breathing technique (n=8). Each intervention was performed twice a day, 5 times a week, for a total of 8 weeks whereupon the change in pulmonary function was evaluated. A spirometer was used to measure FVC, FEV1, and FEV1/FVC. The survey used for this study included the Fagerström Test of Nicotine Dependence (FTND) and the Shortness of Breath Questionnaire (SOBQ). Study results for the comparison within groups showed that in the group that performed both the incentive spirometry and Ujjayi breathing technique, FEV1 improved with statistical significance (p<.05). Furthermore, within this comparison the FEV1/FVC improved with statistical significance. Comparison amongst the groups showed no statistically significant differences in all areas. Following, to effectively increase pulmonary function in young adult smokers, both incentive spirometry and Ujjayi breathing technique should be employed together.

The red ginseng is known to have effects on antioxidativity and cytotoxicity. Nanoscale active substances have various advantages such as improved bioavailability and permeation ability into the cell. However, few studies conducted with the nanoparticles of red ginseng due to its low yield rate and difficulty of manufacturing the product in pilot scale. This study, therefore, investigated the size effects of ultra-fine powder of red ginseng on antioxidativity and cytotoxicity. Red ginseng powder (6, 8, or 158 μm) prepared using a pilot scale was provided by a local company. Antioxidativity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays, and cytotoxicity was tested by methylthiazolyl tetrazolium (MTT) assay. The results of DPPH and ABTS radical electron donating ability IC50 of red ginseng were ranged from 2.27 to 3.34 mg/ml and 2.94 to 3.09 mg/ml, respectively, which were not significantly different between all samples. However, the results of cytotoxicity clearly showed a pattern of decreased toxicity in 6 and 8 μm power compared to 158 μm powder. Unexpectedly, particle sizes of red ginseng did not significantly affect antioxidativity. It is believed that these were related to the process of pilot scale production. These phenomena are also believed to be caused by aggregation of low size power particle that increases water holding capacity. From our result, it is concluded that this range of particle size of red ginseng affected the reduction of cytotoxicity.

The goal of this research was to develop a portable system that could be used to evaluate the quality of milk in real time at a raw milk production site. A real-time portable quality evaluation system for raw milk was developed to enable non-destructive quality evaluation of somatic cell count (SCC), fat, protein, lactose, and total solid (TS) in milk samples. A prediction model of SCC, fat, protein, lactose, and TS was constructed using partial least squares (PLS) and 200 milk samples were used to evaluate the prediction performance of the portable quality evaluation system and high performance spectroscopy. Through prediction model development and verification, it was found that the accuracy of high performance spectroscopy was 90% for SSC, 96% for fat, 96% for protein, 91% for lactose, and 97% for TS. In comparison, the accuracy of the portable quality evaluation system was relatively low, at 90% for SSC, 95% for fat, 92% for protein, 89% for lactose, 92% for TS. However, the measurement time for high performance spectroscopy was 10 minutes for 1 sample, while for the portable quality evaluation system it was 6 minutes. This means that the high performance spectroscopy system can measure 48 samples per day (8 hours), while the portable quality evaluation system can measure 80 (8 hours). Therefore, it was found that the portable qual ity evaluation system enables quick on-site quality evaluation of milk samples.

본 연구는 반추동물의 조사료 자원으로서 거대억새를 개발하기 위한 목적으로 수행되었다. 우리나라에서 새롭게 개발된 품종인 거대억새 1호를 완숙기 이후에 채취하여 in vitro 반추위 발효를 이용해반추위내 pH, 암모니아태 질소, 가스발생량, 휘발성 지방산 생성량 및 건물소화율을 조사하였으며,볏짚과 비교하여 평가하였다. 거대억새는 볏짚에 비하여 유의적으로 높은 반추위내 pH를 나타내었다(p<0.01). 암모니아태 질소의 경우 배양 12시간 이후에는 두 처리구간의 유의적인 차이를 나타내지 않았다 (p>0.05). 배양 6시간 이후 부터는 거대억새의 가스발생량이 볏짚에 비하여 유의적으로 낮게 나타났다 (p<0.05). 휘발성 지방산 생성량에 있어 acetate, propionate, butyrate, valerate 및 총생상량에서 볏짚이 거대억새보다 높게 나타났다. 그러나 iso-butyrate와 iso-valerate에서는 두 조사료원별 차이는 발견되지 않았다. 건물소화율에 있어 배양 12~24시간 사이의 거대억새 소화율이 볏짚에 비하여 유의적으로 나타났다. 결론적으로 거대억새의 이용성은 볏짚의 약 80% 수준인 것으로 나타났다.

The number of abandoned dogs is increasing with the worsening of the economy and the rising of feed value. It was becoming a serious social problem because of the disease transmission and destruction of natural ecosystems by abandoned dogs been wild animal. In order to solve these problems, companion dogs necessary to secure its own genetic information and to establish the systematic tracking system. Using multiplex-PCR method with 27 microsatellite marker (MS marker) divided 3 set, various alleles occurring to 6 dog breed (Labrador Retriever, German Shepherd, English Springer Spaniel, Belgian Malinois, Jindo Dog, PoongSan Dog) make use of markers to determine allele frequency and heterozygosity. MS marker FH2834 and FH2790 have only two allele and most were found in 13 alleles at FH3381 and FH3399. Average heterozygosity of MS marker is 0.534 and especially, heterozygosity represented the highest value of 0.765 at FH3381. So, it was recognized appropriate allele frequency for individual identification and paternity diagnosis in companion dogs. Using multiplex-PCR method with MS marker, various alleles occurring to dog breed make use of markers to deter mine individual identification and paternity diagnosis, traits associated biomarkers and breed-specific marker for faster, more accurate and ways to reduce the analysis cost. Based on this result, a scientific basis was established to the existing pedigree data by applying genetics additionally. Animal registration system is expected to be conducted nationwide in future. The method expects to very useful this system.

The functional response and spatial distribution of parasitoid may be useful for understanding host-parasitoid dynamics because information on these will give knowledge of the host-parasitoid interaction, which could lead to the development of strategy for biological control of insect pests. The functional responses of A. asychis on the green peach aphid and potato aphid was investigated in eggplant leaf-disc arena. As a result, among the three types of functional response, type III best described the A. asychis response to the aphid densities, according to the Bayesian Information Criterion (BIC). Functional response and spatial distribution of A. asychis on green peach aphid, Myzus persicae, was investigated on 5x5 eggplants in the net cage. After M. persicae were inoculated uniformly at the densities of 1, 3, 6, 9 and 12 (per plant) on upper, middle and lower leaves of each 25 eggplants, one mated female of A. asychis was introduced on the central eggplant. Each aphid density was duplicated. A. asychis showed a type II functional response different from that estimated on the eggplant leaf-disc arena. Although the initial distribution of the aphids in the 25 eggplants was uniform vertically and horizontally, the aphids became gradually concentrated on the upper leaves of eggplants, so did immature A. asychis.

An eco-friendly integrated multi-trophic aquaculture (IMTA) farming technique was developed with the goal of resolving eutrophication by excess feed and feces as fish-farming by-products. A variety of seaweed species were tried to remove inorganic nutrients produced by fish farming. However, there have been few trials to use Sargassum fulvellum in an IMTA system, a species with a relatively wide distribution across regions with various habitat conditions, great nutrient removal efficiency and importance for human food source and industrial purposes. In this regard, our study tried to examine feasibility of using S. fulvellum in an IMTA system by analyzing growth characteristics of the species in an IMTA system comprising of rockfish (Sebastes shlegeli), sea cucumber (Stichopus japonocus) and the tried S. fulvellum (October 2011 – November 2012). We also monitored environment conditions around the system including current speed, water temperature and inorganic nutrient level as they may affect growth of S. fulvellum. S. fulvellum in the IMTA system, which were 15.72±5.67 mm long at the start of the experiment in October 2011, grew to a maximum of 1093±271.13 mm by May 2012. In September, seaweed growth was reduced to a minimum of 280±70.43 mm in length. Then, S. fulvellum began to grow again reaching 325±196.19 mm by November 2012. Wet weight of the seaweed was 4.01±1.89 g at the start of the experiment and reached a maximum of 109.26±34.23 g in May. The weight gradually declined to a low of 15.12±8.40 g in September 2012. Weight began to increase once more, rising to 39.27±21.69 g by November. During the experiment, the average velocity at the surface and the bottom was 6.5 cm/s and 3.4 cm/s, respectively. The water temperature ranged 5.0-23.5℃, which was considered suitable for growing S. fulvellum. Results of the study indicated no significant differences in inorganic nutrients between pre- and post-IMTA installation. It was thus concluded that S. fulvellum can be a suitable seaweed species to be used in an IMTA system.

Measurement of amylose content is commonly employed as the predictor of rice quality. Amylose is usually measured by absorbance of the amylose-iodine complex by a variety of ways. However, the effort to standardize the way amylose is measured is still working through the world of rice research. For comparing rice amylose analysis methods in Korea and Japan, major japonica rice-producing countries, rice samples with different amylose contents were selected. The rice samples were harvested, dried and milled at the National Institute of Crop Science, Rural Development Administration in Suwon, Republic of Korea. Amylose contents (AC) were analyzed using Concanavalin A method and modified Juliano methods, as representative methods in Korea and Japan, respectively. The AC of rice starch by Concanavalin A method were ranged 9.4～28.8%, on the other hand, the AC of rice flour by modified Juliano method had a wider range AC, 8.7～41.8%; for example, AC of semi-waxy varieties had lower values and AC of high amylose varieties had higher values in modified Juliano methods, even more than 10%. High correlation coefficients between retrogradation (or gelatinization) and AC using Concanavalin A would indicate that the Concanavalin A method could be more explanatory for evaluating rice quality like gelatinization and retrogradation.