This study was carried out to investigate characteristic pattern of fruiting body of Ganoderma lucidum and their antioxidant activity. Mycelia of all strains were firstly inoculated into potato dextrose agar(PDA) and then transfered to a media of saw dust which contained 20% rice bran. These mycelia of saw dust were then inoculated into oak tree in polyethylene bags which has been sterilized for 8h at 120℃. The polyethylene bags were sent to a growth room for growth of fruit bodies. Antioxidant activities of each fruiting body were investigated by DPPH method.

Maternal hypoxia induced by a variety of exogenous oxidative stresses such as ethanol intake, diabetes, and cigarette during pregnancy provokes the impaired embryonic gene expression and developmental malformations. We investigated the gene expression patterns of the representative selenium containing antioxidant enzymes (selenoproteins) such as cytosolic GPx (cGPx), gestrointestinal GPx (GI-GPx), plasma GPx (pGPx), phospholipid hydroperoxide GPx (PHGPx), and selenoprotein P (SePP) in the cultured mouse embryos under normal or hypoxic (low oxygen state, 5% O₂) condition at embryonic day 8.5 for 2 days using real-time PCR analysis. cGPx, pGPx, and SePP mRNAs were significantly decreased, but GI-GPx and PHGPx mRNAs were remarkably increased in the hypoxic state compared to normal gassing state (p<0.05). These findings indicate that hypoxic condition leads to the unusual expressions of selenoproteins during mouse organogenesis.

Treatment characteristics of benzene were investigated by using a fixed bed reactor system applying a hybrid method over composites of photocatalyst and adsorbent. Various composites were made by mixing photocatalyst with adsorbent, such as activated carbon, activated carbon fiber, and sludge. Performance tests were conducted with benzene concentrations of 1,000～3,000 ppm, Benzene flow rates of 50～100cc/min, and packing weights of 14～24g for the various composite samples. The property of benzene treatment was analyzed concerning BET, SEM, pH, and the conversion efficiency. It was concluded by experimental results that the benzene conversion efficiency of a hybrid method was much higher than that of a photocatalyst only method showing a conversion efficiency range between 13% and 65%. It was also found that the comprehensive feasibility study of the hybrid method would be needed with consideration of various factors including additional expenses.

The yields of the n-hexane-soluble, ethyl acetate-soluble, methanol-soluble and methanol-insoluble fractions from the powdered water extracts were 8.2%, 10.6%, 32.0% and 49.2%, respectively (Table 1). The antifungal activities of water extracts, n-hexane-soluble fraction, ethyl acetate-soluble fraction, methanol-soluble fraction and methanol-insoluble fraction against L. edodes were 23.5%, 26.2%, 41.5%, 25.4% and 2.5%, respectively, at 1000ppm (Figure3). The ethyl acetate-soluble fraction showed much higher antifungal activity than the other fractions. The antifungal activity of the ethyl acetate-soluble fraction against L. edodes at 1000 ppm showed a statistically significant difference in the fractions of n-hexane-soluble, ethyl acetate-soluble, methanol-soluble and methanol-insolub

The yields of the fractions of n-hexane-soluble, ethyl acetate-soluble and methanol-soluble from the P. densiflora sawdust were obtained 1.36%, 2.21% and 4.03% using organic solvents, respectively (Table 1). The antifungal activity of the n-hexane extracts against L. edodes at concentrations 125, 250, 500, 750 and 1000 ppm were 36.5%, 41.2%, 43.1%, 45.4% and 47.6%, respectively (Table 2). The percent of antifungal activity against L. edodes was the greatest for the n-hexane extracts, ranging from 36.5% to 47.6% at a concentrations of 125 ppm to 1000ppm, with the values for all concentrations significantly different from one another. In this experiment, the antifungal activity by the n-hexane extracts was the highest. This result supposed that the n-hexane extracts have not only many more inhibitory compounds against L. edodes than the ethyl acetate and methanol extracts but also the inhibitory compound may be more easily dissolved by n-hexane than by ethyl acetate or methanol.

1. Antifungal activity of water extracts The antifungal activity of the water extracts against L. edodes was proportional to the concentration of the water extracts. The antifungal activity of the water extracts at 1000 ppm was the highest among the tested concentration against L. edodes mycelia. The antifungal activity were significantly different from each concentration of the water extracts. Therefore, it can be concluded that the water extracts of the P. densiflora sawdust have inhibitory compounds to the growth of L. edodes mycelia. 2. Yield and antifungal activity of fractions from water extracts The powdered water extracts was further treated serially using organic solvents in order to separate the water extracts into the fractions of n-hexane-soluble, ethyl acetate-soluble, methanol-soluble and methanol-insoluble. The ethyl acetate-soluble fraction showed much higher antifungal activity than the other fractions. The antifungal activity of the ethyl acetate-soluble fraction against L. edodes at 1000 ppm showed a statistically significant difference in the fractions of n-hexane-soluble, ethyl acetate-soluble, methanol-soluble and methanol-insoluble. And, there was not significantly difference between the antifungal activities of the n-hexane-soluble and methanol-soluble fractions at 1000 ppm. 3. Antivungal activity of fractions of organic solvent extracts The fractions were isolated from each organic solvent by the silica-gel column chromatography and then the fractions were confirmed and divided on the values of Rf by the thin-layer chromatography. The fractions were produced six band of the n-hexane, nine band of the ethyl acetate and eight band of the methanol extracts. The HIII fraction from the n-hexane extracts showed the highest growth inhibition against L. edodes mycelium.

After having inoculated the mycelium of L. edodes on the block medium, the P. densiflora sawdust of the B and C groups were the slowest of their respective groups on day 169.4 and day 169.9 harvesting times. The first harvest of the fruiting body from the Q. variabilis sawdust of the control medium was obtained on day 162.9

양송이 유통에 적합한 필름 포장방법 연구를 위해 저장온도 및 필름 산소투과도에 따른 양송이 품질변화를 분석하였다. 실험에 이용된 필름은 PVC와 OPP(OTR 1,000, 6,000, 10,000, 20,000 mL/m2/day/atm)이고 저장 온도는 4, 8, 12, 20℃로 설정하였다. 분석 결과, 중량감소율은 대조구, PVC, OPP 순으로 높았다. 갓 개열은 온도 별로 결과가 다르게 나타나 8℃ 이상에서 필름 처리구의 갓 개열 억제 효과가 나타났고 12℃에서는 필름 내부의 산소 농도가 낮을수록 억제 효과가 높은 경향이었다. 필름 중 OPP20000은 20℃에서 갓 개열 억제 효과가 나타나지 않았다. 대 신장은 모든 온도에서 전반적으로 필름 처리구가 대조구에 비해 억제되었으나 예외적으로 OPP20000은 대 신장이 대조구보다 오히려 촉진되는 결과였다. 조직의 경도는 필름 중 OPP1000이 다른 처리구에 비해 유의하게 높았다. 버섯의 내외부 색도는 모두 OPP 필름의 버섯이 대조구와 PVC에 비해 색 변화가 큰 경향이었다. 전반적으로 OPP는 중량감소, 갓 개열, 대 신장, 조직 경도에서 대조구에 비해 품질이 유지되는 효과가 있었으나 온도 및 산소투과도에 따라 효과의 차이가 있었고 OPP20000은 조직 생장 억제와 관련된 효과가 나타나지 않았다. OPP1000과 OPP6000은 OPP 중 조직 생장 억제에 가장 효과적이었으나 내부 단면에서 조직손상 및 수침 현상과 같은 장해가 나타났다. 한편 PVC 필름은 중량감소, 갓 개열, 대 신장이 대조구에 비해 변화가 적었고 내외부 갈변은 OPP보다 변화가 적어 전반적인 품질변화가 적은 것으로 분석되었다.

기술이 발전함에 따라 디지털 게임 속 공간이 다채로운 모습으로 구현되고 있으며 이를 체계적으 로 설계하는 것에 대한 중요성은 끊임없이 강조되고 있지만 디지털 게임 공간을 설계하는 방법론을 구축하는 연구는 진행된 바 없다. 이를 위해서는 디지털 게임 속 공간들을 유형별로 분류 및 정의한 연구가 선행되어야 한다. 본 연구는 문헌조사를 통해 디지털 게임 공간을 분류하는 다섯 가지 기준을 제시하였으며, 이에 대한 유효성 평가를 진행하였다. 해당 기준들은 각각 ‘서술형태, 협업 정도, 상호교 환 정도, 공간 동작 차원, 공간 형태’ 이다. 이후 본 연구는 확보된 기준들을 바탕으로 20년간 가장 주 목받은 게임들 분류하여 그 변화 추이를 살펴본 결과 세 가지 대표 공간 유형을 관찰할 수 있었다. 해당 분류 방법론은 디지털 게임 공간 설계 및 구현 인원들에게 디지털 게임 공간에 대한 체계적인 접근을 제공할 것이며, 나아가서 가상경관 설계 방법론을 구축함에 건축 및 조경의 영역에서 활용되 고 있는 각종 설계 방법론의 차등적 적용이 가능하게끔 유도할 수 있을 것이다.

Tea plants growing under natural condition meet various stresses. The tea plants often subjected to adverse condition like low temperature and high light intensity during overwintering. Because tea plant is adapted well to low light intensity, higher light intensity might induce negative effects on tea growth at open field (exposed habitat). We investigated tea plant planted with the seeds of wild tea trees growing at Unsu-ri, Hwagaemyun, Hadong-gun on Nov. in 2008. The experimental field for juvenile tea plants was located in the hill neighboring GSNU at Jinju, Korea. We divided the field into open, semi-shaded, and shaded condition. Light intensity (PAR) at open condition was two times and twenty times higher than at semi-shaded and shaded condition, respectively. Growth of tea plants was highly dependent on light condition, the plants at open field was extremely poor as compared to plants grown at shaded field. Height of plants at open, semi-shaded, and shaded field were 14, 17.3, and 17.8 cm, respectively. Leaf size was also greater in plants grown at shaded field. Photosynthesis-related chlorophyll fluorescence (quantum yield) was also higher in shaded plants. Fv/Fm value of plants grown at open fields was lower by 50% as compared to that of the plants grown at shaded field. Chlorophyll content measured by SPAD meter also indicated higher value in open field plant than shaded plants. Our results suggested the photoinhibition during overwintering period strongly affects the growth juvenile tea plants because there was no significant difference in soil moisture content between open and shaded condition.