Seolan, a new variety of oyster mushroom was developed by Mushroom Research Institute, Gyonggi Province Agricultural Research and Extension Services in 2005. It was bred with mating between monokaryotic strains isolated from KME35124 and F·X3. The major characteristics of the mushroom are showing a lot of pinheadings, the gray-colored and infundibuliform pileus. The optimum temperature for the mycelial growth was around 26∼30℃ and that for the pinheading and growth of fruitbody was around 10∼15℃. Incubation period was required around 25 days with bottle culture and about 23days in Poly Prophylene(P.P) plastic bag culture. The yield were shown high by 138.8g/bottle and 250.9g/P.P bag.

In this study, we prepared porous cation exchange membrane using polystyrene such as, EPS (expanded polystyrene), SAN (styrene acrylonitrile copolymer) and HIPS (high impactive polystyrene). These polystyrenes were sulfonated by acetyl sulfate to make porous cation exchange membrane such as, SEPS, SSAN, SHIPS. SEM was employed to confirm porous structure of membrane, and IR spectroscopy was used to confirm sulfonation rate of ion exchange membrane. Water and methanol content were also increased with amount of sulfuric acid in reactants. SSAN-20 showed the highest value in water and methanol content. Fixed ion concentration and conductivity was also increased with an amount of sulfuric acid in reactants. Methanol permeability for SEPS-20, SSAN-20, SHIPS-20 was found to be 1.326 × 10-5 cm2/s, 1.527 × 10-5 cm2/s and 1.096 × 10-5 cm2/s respectively. From the result of electrodialysis experiment in 0.03 M Pb(NO3)2 aqueous solution, anion exclusion and cation selection effects were confirmed.

Amino acid transporters are essential for the growth and proliferation in all living cells. Among the amino acid transporters, the system L amino acid transporters are the major nutrient transport system responsible for the Na+-independent transport of neutral amino acids including several essential amino acids. The L-type amino acid transporter 1 (LAT1) is over-expressed to support cell growth in malignant tumors. The double stranded RNA-mediated RNA interference (RNAi) analysis can be in a wide variety of eukaryotes to induce the sequence-specific inhibition of gene expression. In this study, we examined the effect of LAT1 short interfering RNA (siRNA) on cell growth using siRNA of LAT1 in the KB human oral squamous cell carcinoma. In the RT-PCR analysis and western blot analysis, the siRNA of LAT1 inhibited expressions of LAT1 mRNA and protein. The uptake of [14C]L-leucine was inhibited by siRNA of LAT1. In the MTT assay, the siRNA of LAT1 inhibited the growth of the KB cells in the time-dependent manner, indicating that the growth inhibition of KB cell by the siRNA of LAT1 is induced by the blocking of neutral amino acid transport mediated by LAT1. These results suggest that the transport of neutral amino acids including several essential amino acids into the KB human oral squamous cell carcinoma is mediated mainly by LAT1. Further, the LAT1 would be a new target for the inhibition of cancer cell growth.

We investigated the electrochemical properties for Langmuir-Blodgett (LB) films mixed with 4-octyl-4'-(5-carboxylpentamethyleneoxy)azobenzene (denoted as 8A5H) and phospholipid(L-α-dimyristoylphosphatidylcholine, denoted as DMPC). LB films of 8A5H monolayer and 8A5H-DMPC were deposited by using the Langmuir-Blodgett method on the indium tin oxide(ITO) glass. The electrochemical properties measured by using cyclic voltammetry with a threeelectrode system, an Ag/AgCl reference electrode, a platinum wire counter electrode and LB film-coated ITO working electrode at various concentrations(0.1, 0.5, and 1.0mol/L) of NaClO4 solution. A measuring range was reduced from initial potential to -1350mV, continuously oxidized to 1650mV and measured to the initial point. The scan rates were 50, 100, 150 and 200mV/s, respectively. As a result, LB films of 8A5H monolayer appeared irreversible process caused by only the oxidation current from the cyclic voltammogram and LB films of 8A5H-DMPC mixture were found to be caused by a reversible oxidation-reduction process.

Activated carbon fiber (ACF) filters are widely used to remove volatile organic compounds (VOCs) in air cleaning devices. The performance of ACF filters could be enhanced combining adsorption process with photodegradation process. In this study, to investigate this enhancement effect, a duct-type reactor was made and TiO2 was i㎜obilized on a co㎜ercialized ACF filter. Benzene, toluene, and m-xylene (BTX) were chosen as target compounds. Removal experiments for BTX were done under different air velocity and upstream concentration conditions. The range of inlet concentration was 200～1,400 ppb and the air velocities were 0.4, 0.7 and 1.0 m/s. Adsorption by an ACF filter alone showed high removal efficiency of BTX, depending on the BTX species, the upstream concentration, and the air velocity. The combination of TiO2 and ACF filter significantly increased removal of benzene which was less removed than other pollutants by an ACF filter alone. It was found that the combination effect was small in removal test of toluene and m-xylene. Removal efficiency in the tested experimental conditions was decreased in order of toluene > m-xylene > benzene.

we studied on chemical ＆ physical variation of cotton waste according to covering materials during outdoor fermentation for oyster mushroom bed culture with cotton waste medium. P.E vinyl and nonwoven thin cloth were used for the covering material. Temperate in the medium ascended up to 75℃ in treatment using nonwoven thin cloth but 60℃ in P.E vinyl treatment for fifth day to seventh day, after that time fell slowly. Before the fermentation, pH of cotton waste medium was 7.2∼7.5 but during the fementation, pH in using nonwoven thin cloth treatment was about 8.9 and pH in P.E vinyl fell 5.1. Ammonium ion concentration in cotton waste medium was 70-85ppm in treatment using P.E vinyl and higher than nonwoven thin cloth. Total carbon content of cotton waste medium was increased but total nitrogen content was decreased while fermentation make progress. when we investigated cotton texture wholly femented by SEM ＆ TEM, cell wall of cotton in treatment using nonwoven thin cloth mostly degraded but it in P.E vinyl was kept unimpaired

톱밥:비트펄프:면실박(50:30:20) 배지의 면실박 대체배지로서 아주까리박, 유채박을 사용한 경우 C/N율은 대조와 유사하였고, 야자박, 코코넛박을 이용한 배지에서는 대조보다 높은 C/N율을 나타났다. 아주까리박, 유채박 사용시 배양일수는 2∼5일, 초발이소요일수는 2일 지연 되었고 수량 또한 대조보다 20g이상 낮게 나타났으며 야자박, 코코넛박 사용시 배양일수는 3일 지연되었으나 수량은 관행재배 128g/병과 비슷하게 나타났다. 경제성 분석결과, 미송톱밥:비트펄프:면실박(50:30:20)의 관행재배에서 면실박 대체배지로 야자박, 코코넛박을 이용시 1000병규모로 연간 8.2회 재배 경우 소득이 각각 2,864천원, 2,733천원으로 관행재배의 2,849천원 대비 101%, 96%의 소득지수를 보였다.

탄성 및 비탄성좌굴 고유치해석법을 이용하여 강절프레임의 보-기둥부재의 유효좌굴길이를 산정하는 개선된 방법을 제시한다. 이를 위하여 먼저 설계기준에 제시된 압축재의 내하력 곡선식으로부터 접선계수이론(tangent modulus theory)에 근거하여 세장비-접선계수(tangent modulus), 응력-변형률 곡선식을 유도한다. 이때 안정함수를 이용하여 보-기둥요소의 접선강성행렬을 얻고, 비탄성 좌굴 고유치해석법을 제시하며 이를 이용하여 유효좌굴길이를 산정하는 방법을 제시한다. 해석예제를 통하여 강절프레임에 탄성 및 비탄성좌굴해석법에 의한 유효좌굴길이 비교결과를 제시하고, 매개변수 연구 결과를 제시한다.

탄성좌굴 고유치해석을 이용한 유효좌굴길이 산정법과 2차 탄성해석기법을 이용하여 축력과 휨모멘트를 받는 강절프레임의 보-기둥부재에 대하여 개선된 좌굴설계법을 제안한다. 이를 위하여 먼저 안정함수를 이용하여 보-기둥요소의 접선강성행렬을 유도하고, 탄성좌굴 고유치해석을 이용한 유효좌굴길이 산정법을 고찰한다. 또한 강절프레임에 대하여 소위 P-Delta 효과를 고려하는 2차 해석법을 제시한다. 해석예제를 통하여 먼저 2차 탄성해석과 기하학적 비선형해석에 의한 결과를 비교하여 2차 해석의 정확성을 검증하고, 강절프레임에 대한 기존의 설계법과 본 연구의 개선된 좌굴설계법에 대한 수치결과를 비교, 검토를 행한다.

This study tries to develop the models of measuring the level of product availability accommodated for features of specific customers dividing customers into VIP customers and general customers. Functions of costs that the models are composed of are cost

국내의 NaCl 함유한 부존자원중 버섯배지화 하기 위하여 내염성 느타리버섯 균주를 선발하고, 선발된 균주를 NaCl 함유 톱밥배지에서 재배하여 생장 및 형태적 특성을 조사한 결과는 다음과 같다. 가. 느타리 버섯 64품종중 NaCl이 3% 첨가한 PDA 배지에서 김제 10호, 농기2-1호, 명월, 병느타리1호, 부평소엽1호, 삼복, 춘추2호 등이 다른품종에 비해 균사생장 및 균사밀도가 양호하였다. 나. 명월은 NaCl 0.5%, 김제10호, 부평소엽1호는 1.0%, 농기2-1호, 병느타리1호, 삼복, 춘추느타리 2호는 1.5%까지 자실체 형성이 가능하였다. 다. 톱밥배지에서 염농도가 높아질수록 배양기간이 길어졌으며 대가 짧아지고 가늘어졌으며 수량이 감소했다. 라. 배지내에 NaCl 함량이 높아질수록 버섯의 K2O, CaO 흡수능은 감소되었으며 자실체내의 NaCl함량은 배지내 NaCl농도가 높을수록 증가하였다.

느타리버섯의 품종 다양화를 위해 단핵균주교잡에 의해 육성된 다조아느타리버섯의 주요특성은 다음과 같다. 가. 균사생장적온은 26∼28℃이고 버섯발생 및 생육온도는 15∼18℃이다. 나. 갓색은 회색이고 얕은 깔대기형이며 발이수가 많다. 다. 병재배시 배양일수는 20℃에서 24일, 초발이 소요일수는 3.4일이며 대형태는 굵고 짧은형으로 농가실증시험에서 병당수량은 140.7g으로 높은 편이었다. 라. 봉지재배시 배양일수는 20℃에서 21일, 초발이 소요일수는 4.2일이며 대형태는 굵고 짧은형이었고 농가실증 시험에서 봉지당 수량은 260.3g을 나타냈다. 마. 버섯의 균일성에 있어서 갓색과 갓형태의 이형개체 발생은 없었고, 균사생장량 및 종균배양기간에서도 지역간 균일한 결과를 나타내었다. 바. 세균성 갈변병 중 Pseudomonas agarici와 푸른곰팡이병 Trichoderma virens에 대해서는 저항성을 나타내었으나 실증재배시 과습한 환경관리 농가에서는 갈변병이 2∼4%발생되었다. 사. 다조아느타리버섯은 균사활력이 강하고 병, 봉지재배 적응성이 높은편이나 발이량이 많아 초기 환기량이 많이 요구되고 후기에는 환기량을 줄여 관리하는 것이 좋다.

This study was conducted to investigate performance of methods for cooking and thawing frozen cooked rice to retard retro-gradation of cooked rice. For this research, That was executed rice's physicochemical and sensory characteristics test through the way of boiling rice or thawing, and found out best way for retro-gradation and taste. This study showed the rice cooked by pressure rice pot has less dehydration during freezing and thawing, and thawed rice by micro wave has less dehydration. Repetition of freezing and thawing resulted increasing of hardness and chewiness, and decreasing of adhesiveness and cohesiveness. The rice cooked by convotherm oven or pressure rice pot has less increased hardness while freezing and thawing. Adhesiveness was superior when rice was cooked by pressure rice pot. The rice thawed by microwave had more hardness than thawed in room temperature.

병재배 느타리버섯의 품종 다양화를 위해 단핵균주교잡에 의해 육성된 흑백느타리의 주요특성은 다음과 같다. 가. 흑백느타리는 X-선 조사된 애느타리1호 단핵균주에 병느타리1호의 단핵균주 교잡에 의해 육성된 품종으로 균사생장적온은 25∼30℃, 자실체 발생 및 생육온도는 14∼16℃이다. 나. 갓색은 흑갈색이고 편편형이며 대는 순백색이고 직립형이다. 다. 배양일수는 20 에서 20일정도이고 자실체는 굵고 짧은형으로 농가실증시험에서 병당 수량은 142.8g으로 높은 편이었다. 라. 색택이 흑갈색이고 생육온도가 17℃이상으로 높으면 회갈색을 나타낸다.

병재배 느타리버섯의 품종 다양화를 위해 단핵균주교잡에 의해 육성된 소담느타리의 주요특성은 다음과 같다. 가. 소담느타리는 애느타리1호와 수한느타리1호의 단핵균주 교잡에 의해 육성 된 품종으로 균사생장 적온은 25∼30℃이고 버섯발생 및 생육온도는 14∼16℃이다. 나. 갓색은 회색이고 얕은 깔대기형이며 발이수가 많다. 다. 배양일수는 20℃에서 20일, 초발이 소요일수는 3일이며 가늘고 짧은형으로 농가실증시험에서 병당 수량은 148.2g으로 높은 편이었다. 라. 균사활력이 강해 배양이 빠르므로 적기에 발이유기가 요구된다.