병재배 느타리버섯의 품종 다양화를 위해 단핵균주교잡에 의해 육성된 흑백느타리의 주요특성은 다음과 같다. 가. 흑백느타리는 X-선 조사된 애느타리1호 단핵균주에 병느타리1호의 단핵균주 교잡에 의해 육성된 품종으로 균사생장적온은 25∼30℃, 자실체 발생 및 생육온도는 14∼16℃이다. 나. 갓색은 흑갈색이고 편편형이며 대는 순백색이고 직립형이다. 다. 배양일수는 20 에서 20일정도이고 자실체는 굵고 짧은형으로 농가실증시험에서 병당 수량은 142.8g으로 높은 편이었다. 라. 색택이 흑갈색이고 생육온도가 17℃이상으로 높으면 회갈색을 나타낸다.

병재배 느타리버섯의 품종 다양화를 위해 단핵균주교잡에 의해 육성된 소담느타리의 주요특성은 다음과 같다. 가. 소담느타리는 애느타리1호와 수한느타리1호의 단핵균주 교잡에 의해 육성 된 품종으로 균사생장 적온은 25∼30℃이고 버섯발생 및 생육온도는 14∼16℃이다. 나. 갓색은 회색이고 얕은 깔대기형이며 발이수가 많다. 다. 배양일수는 20℃에서 20일, 초발이 소요일수는 3일이며 가늘고 짧은형으로 농가실증시험에서 병당 수량은 148.2g으로 높은 편이었다. 라. 균사활력이 강해 배양이 빠르므로 적기에 발이유기가 요구된다.

병재배 느타리버섯의 품종 다양화를 위해 단핵균주교잡에 의해 육성된 진미느타리버섯의 균사생장적온은 25∼30℃, 버섯발생 및 생육온도는 15±1℃이고 갓색은 회색이며 얕은깔대기형으로 발이수가 많았다. 배양일수는 20℃에서 22일, 초발이 소요일수는 4일이며 가늘고 긴형으로 농가실증시험에서 병당수량은 145.9g으로 높은 편이었다. 버섯의 갓색과 갓형태, 이형개체 발생,, 균사생장량, 종균배양기간 등에 대한 균일성도 양호하였고, 세균성 갈변병에 대한 저항성은 소정도로 나타났으나 푸른곰팡이병은 발생되지 않았다. 또한 균사활력이 강하고 15 이하에서는 진한회색을 나타내며 육질이 부드럽고 대가 백색으로 깨끗한 직립형의 특성을 보유하고 있다.

As measures against the environmental pollution, maximum efforts to improve of the environmental pollution have also been made; removal of the contaminated sediments of the bay and the coastal area, monitoring of several heavy metal levels in fishes, shell fishes, sea water and sediment, and so on. The objective of this research is to investigate how much metals are included in the sea water, sediment, fishes and shell fishes in kyeongnam coastal area. Specifically, we are investigating the relationships between the metal included in fishes and sea water, and shell fishes and sediment. and heavy metals and heavy metals respectively. Heavy metals over the studied component and area, the average concentrations of Pb, Cd, Cr and Hg in sea water were 0.0029 mg/L, 0.0005 mg/L, 0.0016 mg/L and ND, those of sediment were 11.9583 mg/kg, 0.2136 mg/kg, 1.9158 mg/kg and Hg 0.0108 mg/kg, those of fishes were 0.4358 mg/kg, 0.0726 mg/kg, 1.1188 mg/kg and Hg 0.0622 mg/kg, and those of shellfishes were Pb 0.6738 mg/kg, Cd 0.2223 mg/kg, Cr 0.5516 mg/kg and Hg 0.0117 mg/kg respectively. In the relationship test, Cd was significant in the relationship between fishes and sea water, and Hg was significant in the relationship between sediment and shellfishes.

This study analyzes the factors involving clinical practice which have an influence on the satisfaction of students majoring in physical therapy at colleges or universities located in the Pusan and Kyungnam area. We investigated using a self-reporting method 305 students receiving a grade point average between 2.0 and 4.0 and who had finished their clinical practice. We also investigated the characteristics of clinical settings which make up the clinical practice, the general characteristics of the students involved and the characteristics of a clinical teaching method performed by physical therapists versus a teaching method by a professor of a university. The number of students divided according to educational background are as follows: 149 people (48.9% of the total group) were 4 year students, 156 people were 3 year students (51.1% of the total group). Sixty-nine students' or 22.6% of the group were men while women consisted of 236 persons or 77.4% of the group. Four year students had a longer clinical practice period than that of the 3 year students (p<.05). An average satisfaction score of students with their clinical practice was 3.84. The satisfaction scores showed no significant difference between genders, educational backgrounds, and grades. (p>.05). There were no significant differences in the satisfaction score of students with their clinical practice was 3.84. The satisfaction scores showed no significant difference between genders, educational backgrounds, and grades. (p>.05). There were no significant differences in the satisfaction scores regarding the student management system among varying gender or educational backgrounds between the 3 year and 4 year programs. The average satisfaction score with the environment of the physical therapy room was 3.35. And there were no significant differences in the physical therapy room satisfaction score based on sex or educational system (p>.05). The most influential factor of determining clinical practice satisfaction was a student management system of the clinical practice (p<.01). The next most influential factors were the clinical practice period (p<.05), size of facilities (p<.01) and relationship with physical therapists (p<.01) (=.554).

A study on the recognition on the mushrooms by elementary school pupils, middle school students and school dietitians were-carried out. The ways of cooking mushrooms by school dietitians were also investigated. Most students and dietitians highly recognized mushrooms as good food. The most favorite mushroom was Flammulina velutipes, the cooking of mushrooms with meat and the hot mushroom soup with meat(Jungol) being the most preferred cooking methods. School ditetitians like to use mushrooms in school lunch and generally use medium or high duality mushrooms. Students, however, do not tend to prefer mushrooms compared to other vegetables. Mushroom dishes that can promote the consumption by students are mushroom bulgogi, mushroom pizza, sweet and sour mushroom in the order.

중국, 일본, 한국인의 3개국의 소비자들을 대상으로 버섯의 소비형태를 조사하였다. 버섯의 주된 구입장소는 중국인은 시장, 일본인과 한국인은 마트였으며 버섯의 이용정도는 한국인보다는 중국인과 일본인이 더 자주 이용하는 것으로 나타났다. 버섯을 구입할때 모양과 가격을 가장 먼저 보는 것으로 조사되었으며 버섯가공품에 대한 인지도는 한국인(45.1%)에 비하여 중국인(68.3%)과 일본인 (62.4%)이 높았으며 주로 구입해본 가공품으로는 중국인과 일본인 조사 대상자들은 버섯절임류(특히, 일본-병조림)가 가장 많았으며 한국인 조사 대상자들은 버섯차를 가장 많이 구입해 본 것으로 나타났다. 식용버섯(느타리, 큰느타리, 표고버섯)의 구입 형태를 조사한 결과 느타리버섯과 큰느타리버섯의 경우는 생것을 선호하였으며, 표고버섯은 건조품을 많이 이용하였고 주된 요리방법은 볶음이었다. 느타리버섯의 경우 갓의 크기에 따른 선호도를 조사한 결과 중국인과 한국인들은 각각 47.9%와 71.2% 갓의 크기가 2-5cm인 버섯을 선택하는 반면 일본인들은 50.6%가 갓이 2cm이하인 것을 가장 선호하는 것으로 나타났다.

Gene delivery is one of the keen interests in animal industry as well as research on gene functions. Some of the in vivo gene delivery techniques have been successively used in various tissues for the gene therapy and transgenesis. Despite intensive efforts, it still remains to overcome problems of limited local and regional administration and low transgene expression. To improve the efficiency of gene delivery, a new procedure was tested. We injected exogenous DNA containing LacZ into the female or male gonads and then pulsed electric field. Electroporated gonads showed positive X-gal staining in many seminiferous tubules of the porcine fetal gonads. Exogenously introduced LacZ genes were also expressed in female porcine gonad. In addition, we demonstrated efficient gene delivery in gonad of adult mouse. Furthermore, we succeed to generate genetically modified germline cells showing GFP and positive X-gal signals. The results suggest that the newly developed gene delivery is an effective way of in vivo transfection in mammals. The developed gene delivery procedure should be useful in producing transgenic animals when combined with primary cell culture and nuclear transplantation.

In this study, we selected supplementary vegetable resources to develpe nutritious supplementary model of korean formula for infants and children, purchased and analyzed it to use with a model. We also referred to an old book about Korean vegetable resources and searched a form of vegetable resources. There are a lot of vegetable resources of various kinds which lied scattered in mountains and fields of our country. As for these, a beyond compare menstruation activity component is becoming clear with a lot of things. It is thought that depth search and study of these vegetable resources and practical application to a infant and child supplemen is desirable. Therefore, we choosed vegetable resources ; a Capsella brusapastoris, a Codonopsis lanceolata, Dandelion, a wild plant, a Tractylis ovata(dried), a Tractylis ovata(native), a Pine plant, a burdock, a JanDae, a Plantain, a HollpDae, which gathered in our country, and we analyzied priximate aomposition of sample (moisture, ash, total carbohydrate, crude fat, crude protein, dietary fiber) and micronutrient contents of sample (calcium, Iron, flavonoids, vitamin A, vitamin C, vitamin E).

The cable staying roof structure of Jeju worldcup stadium should be erected with correct prestressed force that is required by the structural engineer who designs this structure. This study evaluated and adapted the erection process of cable, the erection force and the measurement of cable force for Jeju worldcup stadium. The process of erection is required not only to calculate erection force but also to check structural stability, following process, construction period and using cranes. Considering the site conditions and technical problems, this study can attain successfully the erection of cable staying roof structure of Jeju worldcup stadium with allowable errors.

ADAM은 metalloprotease/disintegrin domain을 가진 transmembrane glycoprotein 으로서 지금까지 30개 이상의 ADAM 및 10개 이상의 ADAMTS 단백질이 알려져 있다. 이들의 기능은 포유동물의 수정시 sperm-egg binding과 fusion, myoblast fusion, integrin과의 결합 등에 직접 관여하거나, TNF-alpha 등의 생체 신호전달물질이 세포로부터 분비될 때에 이들의 구조를 변화시켜 활성화시키는 효소작용, 그리고 dendritic cell differentiation 등에 관여하는 것으로 알려져있다. 그러나 자궁내막 조직에서의 유전자 및 단백질 발현 여부에 관해서는 거의 보고되어 있지 않고 있다. 먼저 RT-PCR 방법을 이용하여 자궁에서 mRNA의 양을 -actin의 양에 대하여 상대적으로 측정한 결과, 임신 1일부터 5일까지는 ADAM-9, 10, 17, TS1의 mRNA 경우 거의 비슷하게 발현되었으며 ADAM-8과 15는 3일째, ADAM-12는 2일째와 4일째에 현저하게 증가하였다 임신 6일부터 8일까지의 자궁조직을 각각 embryo가 착상된 곳과 그렇지 않은 곳으로 나누어 조사한 결과 상대적으로 착상이 된 곳에서 mRNA가 많이 발현 되었으며 특히 ADAM-8, 12, 15의 mRNA의 경우 현저한 차이를 보였다. Immunoblotting 방법을 이용하여 임신 1일부터 5일까지의 단백질의 발현 양상을 조사한 결과 임신 ADAM-8은 3일 이후 감소되는 양상을 보였으며 ADAM-9, 15, TS1은 5일째에 증가하는 양상을 보였다. ADAM-10은 2일째 감소하다가 다시 증가하고 ADAM-12, 17은 3, 4일째 증가하다가 5일째 감소하는 상을 보였다. 한편 임신 6일부터 8일까지는 embryo가 착상된 곳이 그렇지 않은 곳보다 조사된 모든 ADAMs 단백질이 상대적으로 많이 발현되는 양상을 보였다. 이러한 결과로 미루어 ADAMs은 임신 초기 착상과정과 임신 단계에 따른 자궁의 조직 재구성에 중요한 역할을 할 것으로 생각된다.

The survey was conducted from Sep. to Oct. 2001 by questionnaires in order to investigate the patterns and preference of eating out of 321 workers men in JinJu. The frequency of eating out was different with the age of subjects and the purpose for which meals were eaten. However the price of a meal was not different with the purpose of eating out except for purchasing foods at lunch or dinner. Even though small group in 30s and 40s over aged, there are some peoples willing to pay for expensive foods for family or friends. So it needs to develop luxury food items which appeal to these target customer groups. Bibimbab(rice with assorted vegetables) and Naengmyun(cold noodle) had higher ranking for a lunch on the 26-list of famous dishes of commercial restaurents in JinJu. Subjects also preferred Hanjungshik(basic type of Korean menu pattern) and Haemultang(sea food casserole) for a dinner. They had preferences Hanjungshik, Sashimi, Beef Bulgogi for business, social, family meetings. They recommended Hanjungshik, Bibimbab, Beef Bulgogi, Jangeogui(grilled eel), Sashimi to foreign tourists for the best JinJu food with pride. Subjects liked Pizza, Hamburger, Soondae(Korean sausage) for a snack in a day and Pizza, Soondae, Yangnyeumtongdak(spicy fried chicken) were preferred for a snack at night.

Construction of roof structure, cable suspended structure, for Pusan main stadium is adapted a lifting method that is VSL lifting system. 5 processes are practiced for erection of the roof structure including the first lifting process for erection of upper cables and the second lifting process for erection of lower cables. Since all cables of this roof structure with two open spelter sockets are determined their length, some cable were wrong length, he roof structure would be unstable. But, At complete of erection for the roof structure each cable is attained to theoretical tension force with average 4% errors.