The blocked N-terminus and N-terminal sequence ol soybean β-amylase were determined by analyzing the acidic peptides derived on peptic digestion of the enzyme. The acidic peptides were separated from the digest on a Dowex 50×2 column(1×5㎝) and purified by reversed phase-high performance liquid chromatography(RP-HPLC). The major acidic peptide, PEP-1, was a heptapeptide. The N-terminal 7 amino acid sequence of soybean β-amylase was deduced to be acetyl-Ala-Thr-Ser-Asp-Ser-Asn-Met- from the results of sequence analysis of PEP-1 and amino acid analysis of other acidic peptides.

Heatwaves can affect human health and vegetation growth and bring about energy problems and socioeconomic damages, so the analysis and prediction of the heatwave is a crucial issue under a warming climate. This paper examines the production of STCI (Standard Temperature Condition Index) using ASOS (Automated Synoptic Observing System) in-situ observation data for the period of 1979-2020, and an STCI predictability assessment with an RF (Random Forest) model using ERA5 (ECMWF Reanalysis 5) meteorological variables. The accuracy was quite high with the MAE (Mean Absolute Error) of 0.365 and the CC (Correlation Coefficient) of 0.873, which corresponded to 7% to 10% difference for the range of STCI<1.5, and to 1% to 3% difference for the range of STCI>1.5, in terms of the probability density function. Also, we produced gridded maps for the summer STCI from 1979 to 2020 by utilizing the ERA5 raster data for the RF prediction model, which enables the spatial expansion of the ASOS point-based STCI to a continuous grid nationwide. The proposed method can be applied to forecasting of STCI by adopting future meteorological or climatic datasets.

본 연구는 충청북도농업기술원 대추연구소에서 보유 중인 대추 유전자원 중 형태적 다형성을 나타내는 ‘일본’과 주요 대추 유전자원의 형태형질을 조사하고, 대추 육종 소재로서 ‘일본’의 가능성을 확인하기 위해 수행되었다. ‘일본’은 주요 대추 유전 자원과 비교하여 엽과 과실의 크기가 뚜렷하게 작고 당도가 낮았다. 하지만 과실에 폴리페놀 함량이 가장 높았으며, 항산화 능력 또한 가장 높았다. 또한 ‘일본’은 종자형성능이 가장 높았다. 대추 유전자원들의 형태형질을 이용하여 분석한 결과, 지리적 기원에 따라 유전자원들이 분류되었다. 특히 ‘일본’은 한국 및 중국 유전자원들과 명확히 구분되었다. 따라서 종자형성능이 우수하고 기능성 성분이 높은 ‘일본’은 추후 대추 유전연구 및 교배육종을 통한 품종 육성에 유용하게 이용될 수 있을 것으로 생각된다.

Global warming due to the increase of greenhouse gases may significantly affect various aspects of the Earth’s environment and human life. In particular, the impacts of climate change on agriculture would be severe, leading to damages to crop yields. This paper examines the experimental prediction of rice yield in China using DNN (deep neural network) and climate model data for the period between 1979 and 2009. The DNN model built through the process of hyperparameter optimization can mitigate an overfitting problem and cope with outlier cases. Our model showed approximately 38.7% improved accuracy than the MLR (multiple linear regression) model, in terms of correlation coefficient with the yield statistics. We found that the diurnal temperature range and potential evapotranspiration were the critical factors for rice yield prediction. Our DNN model was also robust to extreme conditions such as drought in 2006 and 2007 in China, which showed its applicability to the future simulation of crop yields under climate change.

This paper describes a method for tracking attitude and position of underwater robots. Underwater work with underwater robots is subject to differences in work efficiency depending on the skill of the operator and the utilization of additional sensors. Therefore, this study developed an underwater robot that can operate autonomously and maintain a certain attitude when working underwater to reduce difference of work efficiency. The developed underwater robot uses 8 thrusters to control 6 degrees of freedom motion, IMU (Inertial Measurement Unit), DVL (Doppler Velocity Log) and PS (Pressure Sensor) to measure attitude and position. In addition, the thruster allocation algorithm was designed to follow the control desired value using 8 thrusters, and the motion control experiments were performed in the engineering water basin using the thruster allocation method.

Background: Recently, jujube (Zizyphus jujuba Mill.) has been attracting attention as a fruit, and its cultivation in rain shelter house is increasing to produce the high quality fresh jujube. This study was carried out to investigate the growth and fruit characteristics of jujube according to the types of rain shelter house. Methods and Results: The characteristics of 5-year-old Bokjo cultivar cultivated in 3 types of rain shelter house, multi span rain shelter house with roof vent (Type I), single span house with a column in the center and roof vent (Type II) and single span house with a column in the center and without roof vent (Type III), and open field were examined. The sprouting and blooming period were different among the types of rain shelter house. The diameter of main stem was higher in rain shelter houses than in the open field. There was no a significant difference in fruit number per leaf stem among the types of cultivation. The incidence of fruit cracking in open field cultivation which was 51.2% was much higher than that in Type I 21.6%, Type II 19.3%, and Type III 25.5%. The fruit size and weight in rain shelter houses, especially in Type III rain shelter house were higher than those in the open field and the soluble solids content of fruit in Type I and Type II was higher than in Type III rain shelter house and the open field. Conclusions: The results show that the growth and fruit quality of jujube were improved by cultivation in rain shelter house, and affected by the types of rain shelter house.

Background : Jujube (Zizyphus jujuba. Mill) is a broad-leaved shrub belonging to the family Seagull. Its origin is India and its height is about 5 m. The flowers are gathered in two to three in May-June, with five petals and yellowish green. Leaves are alternate, egg-shaped or long egg-shaped, with clearly visible three veins. The fruit, called jujube, is an elliptical nucleus with the seed wrapped in a solid nucleus. It is 2.5 - 3.5 ㎝ in length, green at first, ripened in brown or reddish brown in September-October. Jujube uses the bud mutation to breed and spreads through grafting. Therefore, there is little difference in phenotype between cultivars. However, because of the lack of research on jujube molecular biology, there is no standard to distinguish the variety at the DNA level. In order to overcome such difficulties and to create a research foundation of jujube, we have developed molecular markers from jujube. Methods and Results : We collected 12 jujube varieties include Bogjo and extracted DNA using CTAB method. The DNA was diluted to 10 ng/㎕ and kept at -20℃. We designed the primer sets using CLC Main Workbench based on DNA InDel regions between the varieties. PCR and electrophoresis were performed to confirm the polymorphism. We designed 26 primer sets from 23 InDel regions. Eighteen of 26 primer sets amplified the amplicon from the primer screening. Eight primer sets were selected for polymorphism assays. All primer sets showed polymorphism. The domesticated cultivars were divided into two groups and the Japanese and Chinese varieties were separated. Conclusion : The InDel markers developed in this study could be good tools to differentiate the jujube cultivars cultivated in Korea.

In recent years, not only the cultivation area of Jujube in Korea but also the number of pest species has increased. The farmers farming environmentally friendly are in great difficulty because there are no effective control devices. This study was conducted to investigate the control effect of the insect screen net on three pest species (Apolygus spinolae, Dasineura sp. and Carposina sasakii) in Jujube orchard when the pests were blocked by insect screen net. For the first and second surveys, the damage rates by A. spinolae were 9.06, 13.95% in 50 mesh, 4.75, 10.17% in 25 mesh, 5.68, 11.84% in 18mesh mesh of insect screen net and 21.6, 36.34% in untreated insect screen net, respectively. The damage rates by Dasineura sp. were 0.54, 0.13% in 50 mesh, 0.93, 2.84% in 25 mesh, 1.05, 13.45% in 18 mesh mesh of insect screen net and 11.1, 26.65% in untreated insect screen net. Carposina sasakii were completely blocked in all the treatments. Damages on Jujube were not observed by insect screen net. Therefore, insect screen net is effective on insect pest control for Jujube.

Intergovernmental Panel on Climate Change (IPCC) provides various prospects of future climate change under the Representative Concentration Pathways (RCP) scenarios using General Circulation Models (GCMs) of Coupled Model Intercomparison Project (CMIP). This paper describes a modified application of Ensemble Bayesian Model Averaging (EBMA) to produce daily mean temperature ensembles using 19 GCMs provided by CMIP. We proposed two types of approach: (1) monthly weighting scheme for a whole area (EBMA.v1) and (2) monthly weighting for each grid point (EBMA.v2), which can take into account the spatially heterogeneous pattern of GCM. For the training period of 1979- 2005 for East Asia, 9,855 sets of daily temperature ensembles (27 years × 365 days) were produced and compared to the ERA-Interim reanalysis data of European Centre for Medium-Range Weather Forecasts (ECMWF), which showed better validation statistics than the general mean and median ensembles. In particular, EBMA.v2 outperformed EBMA.v1 by diminishing the large errors of inland areas where the surface heterogeneity is larger than the ocean. The EBMA.v2 was able to handle the problem of spatial variability by employing monthly and spatially varying weighting scheme. We finally produced daily mean temperature ensembles for the period of 2006-2100 by using the EBMA.v2 under the RCP 6.0 scenario, which are going to be provided on the web.

Background: This study were performed to determine the effect of root pruning of Zizyphus jujuba var. inermis (Bunge) Rehder. Root cutting inhibit vegetative growth and promote reproductive growth as temporarily reducing growth, net assimilation, water potential of leaf and cytokinin level. Methods and Results: The root pruning was treated of the root cutting widths 50, and 80㎝ and the root cutting depths 10, and 20㎝. The amount of root pruning and the number of suckers were the highest in the root-pruning treatment at a width of 50㎝ and a depth of 20㎝. The blooming time was from June 18 to 20, and no difference was observed in the blooming time among the rootpruning treatments. The number of flowers was rather higher in the root-pruning treatment at a width of 50㎝ and a depth of 20㎝ and at a width of 80㎝ and a depth of 20㎝. The percentage of fruit setting was higher in the plants whose roots were pruned at a depth of 20㎝ than in the untreated plants. The fruit size, fruit weight, and sugar content showed no difference among the root-pruning treatments. Conclusions: The results showed that percentage of fruit setting increased with root pruning, while no difference was observed in the growth and fruit quality of plants.

This study was conducted to examine effects of media kinds and container forms for urban agriculture on the growth of herbaceous and woody plants that could be used in construction of urban greenery and landscape gardens. The main objective of the study was to investigate the most efficient culture soil and container form for four herbaceous and one woody plant species. In this study, five different species were examined on three different media kinds [horticultural substrate (HS), decomposed granite (DG), and horticultural substrate + decomposed granite (HS + DG)] with two different container forms [vinyl pot (Ø7.5 cm) and plug tray (20 cells)]. The results indicated that pH values of media of HS and HS + DG were 5.8 and 5.9, respectively while DG was 6.5. HS had the highest electrical conductivity with 0.90 dS･m -1 while HS + DG and DG had 0.39 and 0.08 dS･m -1 , respectively. Especially, most of the species experimented on HS tended to have the highest growth rate in the plant height, the root length, the number of leaves and fresh biomass weight compared to other soil kinds (P<0.05). The growth rate on HS + DG was much higher than DG. Therefore, our results support that HS + DG could be possible to replace with HS. In addition, there had no differences on growth rates between container forms (P>0.05). The use of plug tray could be more applicable than the use of vinyl pot containers, considering growth rates of plants and convenient of shipment on plant production.

급속한 도시화로 인하여 도시 내부에 획일적인 도시 구조물이 건설되었다. 이것은 도시의 경관을 악화시켰다. 이러한 도시 경관 을 개선하기 위한 방법은 도심지 녹지 면적의 확대이다. 하지만 도 시 공간은 다양한 환경조건에 의해 미기후가 만들어지고 이로 인하 여 동일 종 · 지역이라도 개화기의 차이가 발생한다. 이러한 식물계 절의 차이는 도시 경관을 고려한 녹지 조성 시 식물 사용의 어려움 을 준다. 때문에 본 연구에서는 식물계절 예측을 위하여 맹아 80% 수준까지 필요한 적산온도를 구명하였다. 연구 결과 대체적으로 처 리 온도가 고온일수록 맹아기를 촉진하였다. 하지만 식물의 특성에 따라 일정 수준 이상의 고온은 식물의 맹아기를 억제하는 현상이 나타났다. 식물별 개화속도는 산수유, 히어리, 개나리 순이었다. 하 지만 15℃ 수준에서는 일시적으로 개나리가 히어리보다 맹아속도 가 빠르게 나타났으며 20℃에서는 개나리가 히어리보다 맹아속도가 빠르게 나타났다. 그리고 맹아율 80% 수준에 필요한 적산온도는 산수유 169.3±7.7℃, 히어리 226.0±18.0℃, 개나리 279.0±0.4℃ 나타났다.