본 연구는 기존 학자들의 문헌적 연구 성과를 토대로 인도 전통의 차크라 개념을 정의하고 오늘 날 가장 일반적으로 알려진 6, 내지는 6+1의 차크라 체계가 어떻게 성립되었는지를 고찰한다. 차크라는 아직 정확히 규정하기는 어렵지만 만달라, 얀트라와 같이 신들이나 신을 상징하는 만트라들의 원이나 기하학적인 문양을 말하는 것 이다. 그 중 만달라는 보다 큰 규모의 것을 말하고 얀트라나 차크라는 그 속의 일부분인 것이다. 특히 얀트라는 일종의 부적으로 사용할 수 있도록 내구성 있는 것에 만들어진 것이고, 차크라는 특히 원 모양의 신들의 결합을 주로 의미한다. 이런 점에서 만달라를 제사 의례에 사용되는 원 모양의 큰 대(臺)라고 하면 차크라는 그 일부의 요소를 구성하는 것이 다. 그런데 이런 외부적 의례 형태가 신체로 내면화 되었을 때 만달라는 신체이고, 하타요가적 신체의 중심으로서의 차크라는 신체 만달라의 중요 요소를 구성하는 급소라고 할 수 있다. 인도 전통의 하타요가적 신체의 차크라는 원래 초기 탄트라의 외적 의 례가 신체로 내면화 되면서 관상을 위한 하나의 장치로서 형성된 것으로 보인다. 이 내면화 과정은 탄트라에 속한 각 신앙의 교설들에 따른 관념 적이고 상징적인 것이다. 그러므로 탄트라에 속한 각 교설의 차이에 따라 다양한 차크라 체계가 있었지만, 그 중에서도 6, 혹은 6+1 체계가 인도 에서 보편적으로 퍼졌고, 이 체계가 19세기 말에 서구에 전해졌다. 오늘 날 서구의 차크라 체계는 인도 전통의 6, 혹은 6+1 체계에 서구의 오컬 티즘이 결합되어 인도 전통의 차크라 체계와는 상당히 다른 모습을 가지 게 되었고, 이 체계 외에도 다양한 차크라 체계가 있음에도 불구하고 6, 혹은 6+1 차크라 체계만이 유일한 것처럼 인식되고 있다.

1,3,5-benzenetricarbonyl trichloride는 3개의 아실클로라이드가 벤젠고리 1,3,5에 위치한 화학물질로, 높은 가교도 를 통한 물리적⋅화학적 특성을 요구하는 곳에 사용하는 중요한 화학물이다. 특히 일정 기공크기를 갖고 있는 3차원 구조체 형성이 가능하여, 다양한 분리 정제 분야에 사용되고 있다. 하지만 아실클로라이드의 높은 반응성은 반응속도가 빠른 장점을 갖고 있지만, 다른 측면에서는 일정한 성능을 가질 수 있도록 화학적 제어가 어려운 점도 있다. 따라서 본 연구에서는 1,3,5- benzenetricarbonyl trichloride의 화학적 변화가 어떻게 분리막 성능에 영향을 주는지 관찰하였다.

이 연구는 노년기 인지기능 및 생리적 건강상태와 관련 있는 정량화 뇌파의 Alpha 고유리듬 피크 위치 및 진폭의 출현 유무가 정신적⋅육체적 스트레스, 주의집중 등의 뇌파 지표들과 심박변이도 지표 간에 어떠한 관련성과 임상적 의미가 있는지 알아보고자 하였다. 이를 위하여 평균 73세 남녀 노인 76명을 대상으로 전두엽 2채널 뇌파와 1채널 심전도를 동시에 측정 가능한 장치를 이용하여 폐안시 EEG, HRV를 측정하였다. 의미있는 분석 결과는 다음과 같다. 첫 번째, Alpha 고유리듬피크 위치와 상관없이 고유리듬 진폭이 높게 잘 나타난 집단은 낮은 집단에 비해 정신적 스트레스에 대한 저항도는 높고, 육체적 스트레스는 낮게 나타나는 상관성을 보였다. 두 번째, 심박변이도의 HRV index, SDNN, RMSSD 지표는 집단 구분에 상관없이 공통적으로 서로 간 높은 양의 상관성을 보였다. 세 번째, Alpha 고유리듬 진폭이 잘 나타나지 않은 집단에서 좌․우뇌 Alpha 비대칭이 커질수록 육체적 스트레스를 반영하는 Delta/Alpha 비율과 주의집중 저하를 반영하는 Theta/SMR 비율도 커지는 상관성을 보였다. 네 번째, Alpha 고유리듬피크가 느린 리듬쪽에 위치한 집단은 빠른 리듬 쪽에 위치한 집단에 비해 Theta/SMR 비율이 높게 나타나 주의집중도 저하와 관련이 있음을 의미하였다. 따라서 정량화 뇌파의 Alpha 고유리듬 피크 위치 및 진폭의 출현 유무는 노년기 인지기능 및 생리적 건강상태를 반영하는 지표로 활용 가능성이 있음을 시사하였다.

An organophosphorus pesticide, ethoprophos, has been widely used in agriculture to control major insect pests. As ethoprophos is a well-known neurotoxin, its accumulation in soils and groundwater is concerning worldwide. In this study, we constructed an artificial ethoprophos-enrichment soil system, and its active concentration in soils was measured by gas chromatography on 15-days intervals during 90 days after ethoprophos treatment. Furthermore, the changes in bacterial community and microorganisms responsible for efficient bioremediation were investigated while ethoprophos was degraded in soils. From 15 to 60 days after the treatment, ethoprophos was actively degraded in soils and members of genera Collimonas and Sphingobium appeared dominantly in a natural microbial community especially in 60-days-after-treatment soil. We isolated a bacterium EP60845 from this soil sample, showing significant ethoprophos biodegradation activity in vitro. When we challenged EP60845 strain into ethoprophos-enrichment soils (250 mg/kg of soil), most ethoprophos was removed within 5-days. Phylogenetic 16S rRNA gene sequence analysis and biochemical properties by API 20GN kit demonstrated that the EP60845 strain was a novel Sphingobium sp., which could be used as an efficient ethoprophos- degrading agents for bioremediation purposes.

The purpose of this study is to investigate changes in the rental Hanbok market and consumer during the past 10 years. This study was done by analyzing internet news about rental Hanbok and in-depth interview. The results provide basic data that can be used to understand the rental Hanbok market. Results showed the followings: First, the rental Hanbok market has expanded and consists of two types of rental Hanbok; ceremonial and experiential. The experiential Hanbok is new but a big part in rental Hanbok market. It is not existed until 2007 but it accounted for more than 60% of internet news about rental Hanbok in 2016. Second, there is a significant difference in consumer behavior between the two types of rental Hanbok. Ceremonial Hanbok showed consistent consumer behavior between 2006 and 2016. Consumer want to get benefits such as TPO(occasion suitability), economy, exhibition, trendy and exceptionality through renting Hanbok. On the other hand, experiential Hanbok, consumers are motivated by having unique, conformity and sharing memories. Based on these results, different sets of information reveal the unique features of the two types of rental Hanbok. And also needed to develop new designs and marketing strategies for them.

본 연구의 목표는 열 스트레스 하에서 오리사료 내 대사에너지(ME) 수준이 혈액 매개변수에 미치는 영향을 조사하는 것이었다. 총 240마리의 육용 오리 채리밸리((Anas platyrhynchos)를 4처리구 로 완전임의배치 한 후 42일 동안 사육하였다. 처리구는 ME 2900 kcal/kg, ME 3000 kcal/kg, ME 3100 kcal/kg 및 ME 3200 kcal/kg로 구분하 였다. 혈액 지질 프로파일은 ME 3000과 비교할 때 ME 2900이 높았으나 ME 3100과 ME 3200은 낮 았다 (p < 0.05). 혈액 aspartate aminotransferase (AST)와 alanine aminotransferase (ALT) 수준은 ME 3000과 비교할 때 ME 3100과 ME 3200에서 증가하였다 (p < 0.05). 혈액 적혈구와 혈소판 프로 파일은 ME 3000과 비교할 때 ME 3100과 ME 3200은 높아졌으나 ME 2900은 감소하였다 (p < 0.05). 혈액 전해질 가운데, chloride (Cl-) 농도는 ME 3000과 비교할 때 ME 2900에서 낮아졌다 (p < 0.05). 혈액 가스와 PCO2는 ME 3000과 비교할 때 ME 2900에서 감소하였다 (p < 0.05). 혈액 면역물 질(IgG) 수준은 ME 3000과 비교할 때 ME 2900에서 줄어들었다 (p < 0.05). 스트레스 호르몬, 코르티 코스테론은 ME 3000과 비교할 때 ME 2900에서 높아졌으나 ME 3100과 ME 3200은 낮아졌다 (p < 0.05).

Riboswitches are structured RNA motifs that can directly bind specific metabolites. The binding of metabolites further regulates downstream metabolism eliminating the need for any regulatory proteins. We searched for novel bacterial vitamin B1 binding riboswitches in the metagenome of sun-dried saline soil. Soil microbial metagenomes were studied using NGS analysis. A total of approximately 50 Gb of the sequence data was obtained by Hi-seq and 454 GS FLX sequencing, and these sequences were subjected to riboswitch search. Hi-seq generated 614 contigs showing similarity to riboswitches, while 454-based sequencing generated 383 similar contigs. We matched whole metagenome contigs to local BLAST databases constructed using 91 previously known bacterial vitamin B1 thiamine pyrophosphate (TPP)-box motifs, and 11 SAM S-box motifs. Repetitive BLAST comparisons to local BLAST databases with nucleotide sequences from NGS identified 14 novel TPP-box motifs, and 7 S-box motifs respectively from the metagenome contigs. Further, RNA secondary structure analysis with public databases Rfam, and RibEx using these 21 riboswitch candidates revealed one contig, D8PYI to possess the most probable TPP-box structure. We constructed intragenic synthetic riboswitches to investigate whether the TPP-box motif region in D8PYI could harness gene expression in the presence of TPP. Construction of biosensors containing 100~400 bp fragments of D8PYI contigs, and in vivo imaging using the biosensors displayed TPP-specific changes in the expression of a green fluorescence protein reporter. In this regard, the adaptation of in silico riboswitch screening from environmental metagenomes could provide biosensors for detection of specific metabolites.

Simgok Seowon is the commemorative shrine and academy established for scholar Jo Gwangjo (pen name : Jeongam). The shrine (sangnyangsik) and the east and west dormitories (Dongjae and Seojae) were built in 1636. The lecture hall, Imsimnu Pavilion, Sananggak and Munhyanggak buildings were built successively. Simgok Seowon is the only one that survived through the abolishment of seowon ordered by Regent Heungseon in the 19th century. The original seowon was renowned as a representative example showing the typical lecture hall in front and dormitory at the back (jeondanghujae) layout of the Giho School built in 1650. Two archaeological excavations were conducted in the lecture hall courtyard in 2004 and 2007∼2008, which prompted debates the position of the dormitories in respect to the lecture hall, whether the dormitories should be placed in front, or at the back of the lecture hall. Simgok Seowon had been long known as a representative example of the lecture hall in front layout, but the excavations revealed contrasting evidence, suggesting the possibility for a dormitories in front layout. Recent studies and evidence show that the architecture of Simgok Seowon can be grouped into three phases. The first phase was when the shrine and both dormitories were built to the rear of the lecture hall, the second phase was when the lecture hall, Imsimnu Pavilion, Sananggak and Munhyanggak buildings were built, and lastly the third phase when the east, west dormitories were reconstructed in front of the lecture hall. The large scale construction of Simgok Seowon is related to the education based management of the academy by Doam Yi Jae, and the 17∼18th century remains confirmed from the archaeological excavations are evidence of this. Remains for Imsimnu Pavilion, Sananggak, and Munhyanggak buildings have yet to be confirmed, and spaces to the south and east of the seowon are unidentified. Therefore, it is necessary to conduct investigations and research for the unexcavated spaces of the seowon, to conduct in-depth studies and for the effective adaptive re-use of the seowon.

Plant-parasitic nematodes are the most devastating group of plant pathogens worldwide and are extremely challenging to control. In the present study, we have performed a genome wide analysis to identify common genes among four nematode species consisting of root-knot nematodes (Meloidogyne incognita and Meloidogyne hapla), cyst nematode (Heterodera glycines), and free living nematode (Caenorhabditis elegans) respectively. Using their whole genome sequences, we predicted 15,274 genes from M. incognita, 38,149 genes from M. hapla, 8,061 genes from H. glycines and 23,894 genes from C. elegans, where, among the predicted genes, 1,358, 1,350, 1,401, 1,365 respectively from each nematode, code for common groups of proteins. Further, 2,067, 2,086, 1,566, 2,903 genes were recollected using Clusters of Orthologous Groups (COG) database. Under our search criteria, a total of 800 common genes were identified in all the four studied nematode genomes. The most annotated conserved genes were obtained from four different species using Basic Local Alignment Searching Tool (BLAST). Uni- Prot Taxon identifier database was used to elucidate their taxonomic classification such as 698 genes under kingdom Metazoa, 660 genes confined to Nematoda, 290 genes in Chordata and 660 genes falling under class Chromadorea. The biochemical characterization of proteins expressed by these genes was examined using Pedant-Pro sequence analysis. The protein length, molecular weight, isoelectric point (pI), and transmembrane domain of the coded proteins were at a range of 300 to 999 amino acids (40.9%), molecular weight of over 100 kDa (96%), pI from 4.5 to 5.5 (27.6%) and 0 (56.6%), respectively. To classify protein function, the obtained BLAST hits were assigned to Gene Ontology classification scheme. The fractions of protein function were distributed as cellular component, biological processes and molecular function of the cell (22.2%), multicellular organism process (15.8%) and binding (48.3%), respectively. The current study provides an excellent resource for nematode functional genomics studies, which can be utilized further for studies on role of genes involved in nematode biological processes.

Human tissue-type plasminogen activator (t-PA) is responsible for fibrin-specific plasminogen activation and plays a key role in fibrinolysis thereby aiding breakdown of blood clots in the vasculature. In the present study, in order to develop a system for production of recombinant st-PA and t- PAHis6 proteins in transgenic rice seeds, a DNA fragment encoding t-PA gene was selected and cloned to a plant binary vector (pMJ21) harboring a rice GluB1 promoter, an N-terminal signal peptide of the rice glutelin B1 protein and a Pin II terminator. The constructed plasmid was transformed into Agrobacterium tumefaciens LBA4404 (pSB1) to facilitate introduction into rice callus. The insertion of the st-PA and t-PAHis6 genes into the genome of transgenic rice seeds and their transcripts were confirmed using PCR, and Southern blot as well as RT-PCR, respectively. The highest level of recombinant st-PA expression as determined by enzyme-linked immunosorbent assay (ELISA) was found to be 2,916 ng/total soluble protein (mg) in transgenic rice seeds. The amount of recombinant proteins expressed in transgenic plants was estimated to range from 634 ~ 2,916 ng/TSP mg (st-PA) and 925 ~ 2,640 ng/TSP mg(t- PAHis6), respectively. Immuno-blot analysis of transgenic rice seeds revealed single bands of approximately 68-kDa representing recombinant st-PA and t-PAHis6 proteins. These results demonstrate the expression and in vivo activity of recombinant st-PA and t-PAHis6 in transgenic rice seeds. This study is a promising endeavor for production of recombinant pharmaceutical proteins using rice seed system.

The purpose of this paper is to find an limitation to detect the defect of damaged asphalt pavement structures for infrared thermography. We use heat source of a natural light to detect the defect efficiently. The heat source was applied to the asphalt specimens. Four asphalt specimens were used: one was the asphalt containing depth of 1cm internal timber, two was the asphalt containing depth of 2cm internal void, Three was the asphalt containing depth of 3cm internal timber and four was not the asphalt containing internal timber. It was found that the depth of 3cm internal timber could be detected by this method. In addition, we used the image processing to make the damage zone displayed clear in the image obtained from the thermographic operation.

본 연구는 다양한 냉동방법(강제송풍식냉동, 극저온냉동, 일반냉동)과 해동방법(자연해동, 유수해동, 초음파해동, 전자레인지해동)을 조합하여 처리했을 때 표고버섯의 물리적 품질에 미치는 영향을 관찰하고, 표고버섯의 품질을 유지하는 가장 효과적인 냉·해동 공정을 탐색하는 것이 목적이었다. 급속냉동법(강제송풍식냉동, 극저온냉동)으로 처리한 경우 표고버섯의 해동감량, 보수력, 수분함량은 큰 변화 없이 품질을 유지 하는데 효과적인 것으로 관찰되었다. 특히 보수력은 저속냉동(일반냉동) 및 저속해동(자연해동) 처리의 유무가 영향을 미치는 것으로 나타났다. 전단력의 경우 냉동방법에 의한 영향보다, 전처리 및 해동방법에 더 영향을 받는 것을 판단된다. pH 및 색도는 냉동방법 및 해동방법에 따라 거의 차이가 없는 것으로 관찰되었다. 표고버섯의 물리적 특성을 효과적으로 유지 할 수 있는 처리 조합은 급속냉동(극저온냉동) 및 급속해동(유수해동, 초음파해동, 전자레인지해동)을 한 경우이며, 또한 유지하고 하는 물리적 특성에 따라 냉동 및 해동방법을 다양하게 조합하여 처리한다면, 식품의 품질을 보존하는데 더욱 효과적일 것으로 사료된다.

In this study, a real-scale specimen of Steel-Confined Prestressed Concrete (SCP) composite girder was tested in a three points bending. Furthermore, vibration tests were performed to verify the dynamic stability of the SCP composite girder for railway bridges. The results of the experimental test were similar to stable rigidity behavior until the static actuator limit load (6300 kN). That is, the SCP composite girder showed a stable rigidity behavior in the ultimate load (3,174 kN) as well as the service load (1,076 kN). Finally, the possibility of resonance of the SCP composite girder has been found to be low.