본 연구에서는 미분 가능한 함수가 Taylor 전개로 표현되고 그 계수들은 주어진 함수와 미분에 대한 근사값을 제공할 수 있다는 점에 착안하여 m차 Taylor 다항식을 구성하고 이동최소제곱법을 이용하여 그 계수들을 구했다. 계산된 근사함수와 미분을 콜로케이션 개념을 바탕으로 균열 문제를 포함하는 고체문제에 대한 지배 미분방정식에 적용하여 차분식 형태의 이산화된 계방정식을 구성하였다. 본 연구의 해석기법은 격자망(grid)에 의존적이고 근사함수가 없는 유한차분법과 형상함수의 미분과 약형식의 적분산정, 필수경계조건 처리가 어려운 Galerkin법 기반의 무요소법의 단점을 효과적으로 극복한 새로운 수치기법이다.

돌나물의 수확후 저장온도가 저장성에 미치는 영향을 분석하기 위하여 저장온도에 따른 생체중, 색상차, 엽록소 함량, 수분함량, 외관상 변화 등을 조사하였다. 생체중은 저장온도에 따라 8.7-25.3%의 감소를 보였으며, 저장온도가 높을수록 생체중 감소율도 높았다. 계통에 따른 생체중 감소는 포항지방종이 가장 컷으며, 완주 지방종의 변화가 비교적 적었다. 외관상 품질은 20℃에서 4일경, 10℃에서 6일경, 5℃에서 8일경부터 하위 잎에 변색이 나타나기 시작하였으며, 시간이 지남에 따라 서서히 부패하기 시작하였다 SPAD 값으로 비교한 엽록소 함량은 20℃와 10℃에서는 4일과 6일 후부터 급격한 감소를 보였으나, 5℃에서는 변화가 적었다. 잎의 색도 변화에서 명도를 나타내는 L값과 녹색-적색을 나타내는 a값은 저장온도가 높을수록 저장일 수에 따라 유의하게 증가하였다. 저장 후 10일째에 변색이나 부패한 돌나물의 비율은 저장온도가 높을수록 증가하였으며, 이는 수확시의 수분함량과 유의한 상관 관계를 보였다(P=0.01). 계통간에는 완주 지방종이 비교적 저장성이 높았으며, 군산, 완도, 포항 순으로 부패율이 높았다. 따라서 돌나물을 스치로폼 접시에 랩포장 할 경우, 5℃에서는 수확 후 8일까지 상품성을 유지한 상태로 저장이 가능하였다.

밀양 23호/기호벼 유래 164 RILs(F17)을 이용하여, 종자의 휴면과 관련이 있는 수발아 저항성, 건열 저항성 및 종자수명을 검정하고, 이들 상호간 상관관계를 조사하였다. 출수 40일째에 밀양23호와 기호벼의 수발아율은 0%, 7%이었고, M/G RILs은 0~50.9%의 범위를 보였으며, 평균 3.2%였다. 90℃, 24시간 건열처리후 밀양23호와 기호벼는 99.2%, 37.6%의 발아율을 보였고, T50은 2.7일, 12.9일로 유의한 차이를 보였으며, M/G RILs의 평균 발아율은 72.4%였다. 상온저장 한 종자수명은 밀양23호는 수확후 3.5년까지도 80% 이상의 종자 발아율을 유지하였으나, 기호벼는 18개월 후부터 급격히 발아력이 감소하여(30%이하), 42개월 후 발아력을 완전히 상실하였다. M/G RILs의 종자활력은 수확후 42개월까지 71계통이 90%이상의 발아율을 보였다. 수발아 저항성, 건열 저항성 및 종자수명은 밀양 23호와 기호벼 간에 뚜렷한 품종간 차이를 보였으며, M/G RILs은 양친의 중간값에 가까운 평균값을 보였다. 건열저항성과 종자수명 간에는 고도로 유의한 정의 상관관계를 보였고, 수발아와 종자수명, 수발아와 건열저항성 간에는 부의 상관관계가 성립하였다.

열화학 기상 증착법을 이용하여 암모니아 처리에 따른 촉매 금속의 표면형태와 탄소 나노튜브의 성장을 조사하였다. 암모니아 처리의 열화학 과정의 조절에 의해 고르게 분산된 수직 성장된 탄소 나노튜브를 얻었다. 탄소 나노튜브 합성시 암모니아처리는 수직성장 및 고밀도 성장에 중요한 과정으로 그 역할에 대해 알아보았다. 고밀도의 수직 배향된 탄소나노튜브의 구조와 형태는 주사전자 현미경과 투과전자 현미경, 라만을 이용하여 관찰하였다.

본 논문에서는 요소를 사용하지 않은 수치해석기법인 무요소법 중에서 다중해상도(multi-resolution)특성이 내재되어 있는 Reproducing Kernel Particle Method (RKPM)의 이중스케일 분해기법을 사용하여 RKPM의 형상함수를 상단성분과 하단성분으로 분리하고 이를 3차원 선형탄성해석과정에 적용하여 von Mises 응력장의 상하단성분을 유도하였다. 유도된 응력장의 상단성분을 이용하여 후처리과정을 거치지 않고도 응력의 고변화도 부위를 손쉽게 파악할 수 있는 기법을 개발하였으며 이를 이용한 효율적인 적응적 세분화기법의 적용가능성을 연구하였다. 대표적인 2차원 및 3차원 응력집중 문제에 적용하여 응력집중부위를 파악하고 간단한 적응적 세분화과정에 따른 절점추가를 통하여 해의 정도 향상을 파악해 본 결과, 본 연구에서 개발된 기법이 응력집중부위를 정확히 판정할 수 있었으며 효율적인 적응적 세분화기법의 유용한 도구로서 활용될 수 있음을 검증하였다.

This paper was performed to investigate the effect of dietary fiber and Chlorella added diets on serum composition of blood and histopathological change of liver and kidney in male rats. As the result, pectin added diets have an effect of decrease a serum, cholesterol and lipid of the tissue.

별불가사리(Asterina pectinifera Muller and Troschel)는 우리나라 전국 연안에서 흔히 볼 수 있는 토속종으로, 패류 양식장에 피해를 주는 불가사리류 중의 하나이다. 별불가사리 퇴치를 위해 대부분 건조하여 비료로 활용하고 있으며, 고부가가치 창출을 위한 다양한 연구가 진행되었으나 실제 활용은 미미한 실정이다. 따라서, 별불가사리로부터 피부 유용성분을 밝혀 새로운 활용 방안을 모색하고자 하였다. 성분연구를 통해 별불가사리로부터 2종의 polyhydroxysteroid와 1종의 saponin을 분리하였으며, 이의 구조를 각각 5α-cholestane-3β,6α,7α,8,15α,16β,26-heptol, 5α-cholestane-3β,4β,6α,7α,8,15β,16β,26-octol 및 asterosaponin P1으로 동정하였다. 이 물질들의 피부 효능을 확인한 결과 asterosaponin P1이 표피 줄기세포의 증식을 촉진시키고, 각질형성세포에서 히알루론산을 합성하는 효소인 hyaluronan synthase-2와 hyaluronan synthase-3 유전자의 발현을 증가시킴을 확인하였다. 또한, asterosaponin P1은 섬유아세포에서 진피의 주요 콜라겐인 type 1 콜라겐의 생합성을 촉진하는 효능을 보였다. 이상의 결과들로부터, 별불가사리로부터 분리한 asterosaponin P1은 노화에 동반되는 피부 증상을 개선하는 항노화 화장품 소재로 활용될 수 있을 것으로 판단된다.

Background : Rehmannia glutinosa is a perennial herb belonging to family Scrophulariaceae. This study was breeding of R. glutinosa cultivar with insect tolerance and high-yielding. Methods and Results : ‘Chunggang’ is developed by the medicinal crop breeding team of National Institute of Horticulture and Herbal Science (NIHHS), Rural Development Administration (RDA), during the period from 2005 to 2017. The reproduction of Rehmannia glutinosa has been accomplished mainly by vegetative propagation with its seedlings. This cultivar was selected from seedling of RG10. The plant type of Chungang is some rising from ground. Regional yield trials conducted at three site from 2016 to 2017. The root yield of ‘Chunggang’ was 22.3 ton per hectare, which was increased 11% compared with ‘Jihwang 1’ (check variety). Also, ‘Chunggang’ has higher insect tolerance compared with ‘Jihwang 1’. Conclusion : ‘Chunggang’ is a insect tolerance and high-yielding Rehmannia glutinosa cultivar.

Background : This study was conducted to investigate the effect of soluble silicate and germanium on the characteristics of soil and growth in ginseng (Panax ginseng C. A. Meyer) cultivated in blue-white plastic houses. Methods and Results : Seedlings (n = 63 per 3.3 ㎡) of Korean ginseng cultivar were planted on April 10, 2015. Shading material of plastic house [8 m (W) × 3.5 m (H) × 50 m (L)] was blue-white vinyl. From May to September of each year, 1,000 times diluted sol. of germanium and silicate and these mixed solutions were drenched once a month. The chemical properties of the soil are as follows : pH, EC, organic content, and P2O5 of the soil were within the proper range, and the Mg content of the Ex-cations was lower than the standard value in 3 treatments except treatment of germanium 1,000 times. The total nitrogen content was 0.13 - 0.14%, which was higher than the optimum value. The number of soil microorganisms (Actinomycetes etc.) was higher in the treatment than in the non-treatment of inorganic components. The photosynthetic rate of ginseng leaves was 2.25 μmole CO2/㎡/s in the non-treatment and 2.91 to 3.17 μmole CO2/㎡/s in the inorganic treated. The growth characteristics of the aerial parts (leaf and stem) of the ginseng plants were generally better than that of non-treatment. Especially, in the treatment with silicate 1,000 times showed better growth, leaf area and leaf width growth. The growth of the underground part (root weight etc.) were also better than non-treatment. Specially, at 1,000 ppm treatment with soluble silicate, the root length and diameter were longer and the weight was was 72.2 g per plant. The ginsenoside content of each treatment is under analysis. The disease incidence (Alternaria panax etc.) rate was 20.1% in the untreated and 6.4% to 10.0% in the inorganic components treatment. Conclusion : By soil drench of inorganic compononts such as soluble silicate in ginseng blue-white plastic house cultivation, the growth of the aerial and root parts in ginseng were more improved than non-treatment. There are a lot of soil microorganism and less disease.

Background : The cultivation area of Rehmannia glutinosa Liboschitz is 156 ㏊ ('16), which is one of my representative medicinal crops. However, since it grows by roots, the damage of the repeated cultivation and the degeneration of the seedstock are increasing. To solve these problems, we are developing new varieties every year by testing the regional adaptability of high quality system. In recent years, as the quality and quantity of new varieties have been proven to increase, the need for continuous development of excellent varieties is increasing. Methods and Results : The test material used a jihwang-1 as standard variety, the system was planted in 3-year (RGES 20), 2-year (RGES 21, 22, 23) and 3-year (RGES 24, 25). The root lengths were cut at intervals of about 2 to 3 ㎝ and immersed for 20 minutes in a fludioxonil suspension concentrate (1,000-fold dilution) and shaded for 1 day. Sowing was planted on April 30th, and planting interval was 30 ㎝ × 15 ㎝. Growth investigation was carried out on the overground growing (plant length, leaf length, leaf number, plant type, etc) and degree of generation of pests. As a result of the growth investigation, the third year RGES 20, the second year RGES 22, and the first year RGES 25 were excellent. RGES 20 was upright type and superior in overground growing, RGES 22 had strong pest resistance. RGES 25 was characterized by strong at high temperature, but growth is not as good as other high quality system. Conclusion : Recently, the difficulty of cultivation of zucchini has been increasing due to abnormal weather. Especially in this year, high temperature of 35℃ or more continued for about 30 days, and the need to cultivate varieties resistant to high temperatures is increasing. Therefore, it is expected that RGES 25 can be registered as a promising strain that is resistant to high temperature if the yield is corrected.

Background : Medicinal crops the importance of good agricultural practice (GAP) cultivation is increasing, but the number of GAP-certified farms is decreasing because of the decrease and undeveloped application of medicinal crops. In the case of Artemisia capillaris Thunb., GAP cultivation is difficult because there is no registered application agricultural pesticides. The insect pests that occur mainly in apples are Aphis gossypii. Especially, if the high temperature is continued as in this year, the incidence rate increases rapidly, which greatly affects the growth delay. Therefore, this study was conducted to select the application agricultural pesticides of A. gossypii for the stable production of GAP in A. capillaris. Methods and Results : Three-years-old (Jangsu, Jinan) A. capillaris were used as test materials. The treated medicines (2,000-fold dilution) were acetamiprid wet table powder (WP), clothianidin suspension concentrate (SC), imidacloprid WP and thiacloprid SC. To investigate the phytotoxicity, 2 times of medicine (1,000-fold dilution) was treated. The medicinal effect was confirmed at 3 and 7 days after the treatment of the medicinal stuff, and the phytotoxicity was investigated from symptoms appearing in the stem and leaf. As a result of treatment, in Jinan area, the treatment rate was more than 80% in all treatment chemicals on the 3 day after treatment, but on the 7 day, acetamiprid WP and clothianidin SC remained more than 80%. In the jangsu area, only the imidacloprid WP was less than 80% on the 3 day of treatment, but on the 7 day, acetamiprid WP and imidacloprid WP were maintained more than 80%. In particular, thiacloprid SC had a high control rate of 93% on the 3 day, but as the fell rapidly to 32% on the 7 day, It is considered that the persistence of medicinal effect is low. Conclusion : The results of medicines treatment for A. gossypii control showed that the control efficacy was higher than 80% until the 3 day of treatment, but the efficacy decreased at 7 day. The medicines with high control rate were acetamiprid WP, and the control rate was maintained more than 80% from 3 day to 7 day. Therefore, appropriate conclusions were obtained for the applied medicine.

Background : Cirsium japonicum is a perennial medicinal crop, characterized by the fact that it does not bloom in the first year. Therefore, annual C. japonicum leaves and roots are used as medicinal materials, and biennial do not harvest for seed gathering. In addition, thistle has a very low germination rate, which means that it takes a lot of seed in planting. In order to solve these problems, we conduct to determine the optimum maturity stage and the location of the inflorescence in C. japonicum. Methods and Results : The test material was a biennial C. japonicum. The harvesting time was sampled at intervals of 10 days (7 treatments) from the beginning of June to the beginning of August, and the location of the inflorescence was sampled from 1 to 3 inflorescence, 4 to 6 inflorescence, and 7 to 9 inflorescence. The buds collected for seed selection were dried for 10 days and used as germination test seeds. Seeds to investigated germination rate were soaked in 70% alcohol for 10 minutes and then washed with distilled water. Then, 20 seeds were arranged in a petri-dish, After standing at 25℃ for 7 days, germinated seeds were counted. As a result of the germination rate, at the beginning of June, the germination rate of seeds collected from 1 to 3 inflorescence was the highest at 35%. In the middle of June, it decreased by 2 times to 3.8%, and the germination rate tended to decrease as location of the inflorescence was lowered. In late June, it decreased to 1 - 2%, and most seeds collected after July did not germinate. The decrease of germination rate according to the harvesting time and the location of the inflorescence, it is considered that sufficient nutrients are delivered in the early stage but the number of immature seeds is decreased as the amount of nutrients is decreased. Conclusion : Appropriate sampling methods for the production of high quality seed in C. japonicum, the germination rate could be maximized by sampling from 1 to 3 inflorescence at the beginning of June. but, sufficient nutrient supply is required to increase the ripened seeds. Therefore, it is necessary to investigate further research on the additional fertilizer technology.