There are three kinds of Pleurotus eryngii : P. eryngii var. eryngii (P. eryngii), P. eryngii var. ferula (P. ferula : A-Wei-Mo) P. eryngii var. nebrodensis (P. nebrodensis : Bai-Ling-Gu). We bred P. ferula using Di-Mon mating and physiological assay and selected as follow (1) ‘Beesan No.1’ produced high yields, (2) ‘Beesan No.2’ was excellent morphological shape and anti-adipogenic Activit, (3) ‘Ergo’ included high ergothioneine such as anto-oxidant material, (4) ‘GW10-45’ included highest ergothioneine such as anto-oxidant material, (5) ‘Maeksong’ included high ergothioneine and effect of anti-inflammatory. We suggest that there are able to food-medicine materials.

본 연구에서는 버섯추출물 (42개)의 in vitro 항알레르 기 효능탐색을 위해 랫드비만세포 (RBL-2H3 cell)에서 면역글로불린 (IgE)가 매개한 탈과립에 대한 저해 효과를 실험하였다. 이를 위해 anti-DNP IgE 및 DNP-HSA에 의 해 알레르기반응이 유발된 랫드비만세포에서 버섯 추출물 의 IL-4과 β-hexosaminidase 분비량에 대한 저해활성과 세포생존에 대한 영향이 분석되었다. 실험결과, IL-4 분비 에 대해서는 팽이버섯 물추출물 등 5개의 버섯 추출물이 20% 이상의 우수한 저해효과를 나타내었으며, β- hexosaminidase 분비에 대해서는 영지버섯의 물추출물 등 8개의 버섯 추출물이 20% 이상의 비교적 우수한 저해활성 을 나타내었다. 세포증식에 대해서는 잎새버섯의 물추출물 등 대부분의 버섯 추출물이 우수한 세포증식효과를 나타 내었다. 팽이버섯의 물추출물과 상황버섯의 물추출물은 β- hexosaminidase 및 IL-4 분비에 대해 모두 비교적 우수한 저해효과를 나타내었다. 추가로 2, 10, 50 ug/ml에서 실험 된 영지버섯, 편각영지버섯, 눈꽃동충하초, 상황버섯 그리 고 느타리버섯의 물추출물들은 β-hexosaminidase의 분비 량을 농도-의존적으로 감소시켰다. 이상의 결과를 살펴볼 때, 이들 in vitro 항알레르기 효과를 나타낸 버섯 추출물 들은 추가실험을 통해 항알레르기 소재로의 활용성 검토 가 필요하다고 사료되었다.

This study was carried out to investigate comparision anaylsis of metabolites in mandarin leave inoculated by Elsinoe fawcetii. For the analysis of metabolites, we performed using by UPLC-MS spectrometry in gradient eluent condition by acetonitrile and water. Flow rate was 0.4ml/min. Instruments conditions were followed by detection ion mode(positive ion), scan range(MS:m/z100-1000), spray voltage (4kV) ,capillary voltage(35v), capillary temp(300℃). Several flavones were detected in inoculated pathogen compared to control.

Three edible mushrooms; "Gumbit"(Pleurotus cornucopiae var. citrinopileatus) and Noeul"(Pleurotus salmoneostramineus) and Tricholoma spectabilis which are widely distributed in China, Japan and Korea. For the study of anti-aging activities, we were performed on adriamycin-induced cellular senescence model. Human dermal fibroblasts(HDFs) and human umbilical endothelial cells(HUVECs) treated with adriamycin for inducing aging were used for cytotoxicity and senescence-associated beta-galactosidase(SA-β-gal) activity assay.

Cordyceps militar is (Clavicipitales) is an edible mushroom which is widely distributed in China, Japan and Korea. Various phytochemical constituents, cordycepin, homocitrully laminoadenosine and sterols have been reported from this source and a wide range of biological activities, including antimicrobial, macrophage activation, anticancer, immune modulatory effects were studied. In a continuing search for bioactive constituents from Korean mushrooms, we performed a phytochemical investigation of the MeOH extract from the fruiting bodies of C. militaris. By repeated column chromatographic separation of the extract, fourteen compounds, were isolated. The identification and structural elucidation of the compound was based on NMR spectral data. anti-aging activities were performed on adriamycin-induced cellular senescence model. Human dermal fibroblasts(HDFs) and human umbilical endothelial cells(HUVECs) treated with adriamycin for inducing aging were used for cytotoxicity and senescence-associated beta-galactosidase(SA-β-gal) activity assay.

Thousands of mushrooms grow wild in earth and fifteen hundreds of mushrooms in Asia. Mushrooms has been used for food ingredients since early times. Recently it is being used as a good pharmaceutical material or functional bio-material. Because of unknown function, it is worth while to examine the wild mushrooms. We performed experiments with 15species(Russula sorroria, Lactarius japonicum, Tylopilus vireus, Amanita, Russula foetens, pseudoporphyia, etc.) of wild mushrooms. With Methyl alcohol extracts of wild mushrooms, we have investigated the cell growth inhibition by the MTT assay and antioxidant effects by DPPH. Among them, showed excellent results. In this meaning, Forward a variety of biologically research(antioxidant, anticancer and anti-inflammatory effects) is needed.

Mushrooms has been used for food ingredients since early times. Recently it is being used as a good pharmaceutical material or functional bio-material. Especially Ganoderma lucidum, Phellinus and Inonotus are used for medicinal purposes. Ganoderma is a basidiomycete white rot fungus which has been used for medicinal purposes for centuries particularly in China, Japan and Korea. A great deal of work has been carried out on Ganoderma lucidum. The metabolites consist of mainly polysaccharides and terpenoids. At present, Ganoderma is a health food supplement to support cancer patients, yet the evidence supporting the potential of direct in vivo anticancer effects should not be underestimated. the aim of this study was to investigate the anti-cancer effects and anti-inflammatory of Ganoderma lucidum in some kinds of human cell lines (Gastric:AGS, liver:HepG2, breast:MCF-7, lung:A549, uterine:Hela, Raw 264.7) we are examined the cell viability by MTT assay and effects of anti-inflammatory by NO assay. Anticancer and anti-inflammatory effect in the 10strains were selected.

Ganoderma lucidum, a popular medicinal mushroom, has been used in China for longevity and health promotion since ancient times. Recently it is being used as a good pharmaceutical material or functional bio-material. Total 106 strains was cultured at temperature(25 ℃, 30 ℃ ,35 ℃) to study cultural properties of Ganoderma lucidum. Then the mycelium growth was digitized in the average diameter. With the result, Ganoderma lucidum strain has the optimum growing temperature at 25 ℃ with 25 strains, 30 ℃ with 70 strains and 35 ℃ with 11 strains. The 84strains were cultivated in wood for investigating the cultivation properties of Ganoderma lucidum. With the result, 70mm~90mm of the pileus diameter (major axis, mm) and 70mm-80mm of pileus diameter (minor axis, mm) was measured after one year cultivation. And 1,000-2,000 ㎟ of strain took the most the area (major axis x minor axis). The 30-40mm of stipe length,1time of harvesting and volume was 200g~400g took the most. 10cm-15cm of the pileus diameter (major axis, mm), 5cm~7.5cm of pileus diameter (minor axis, mm) and 15-20mm of thickness was measured after 2 year cultivation. And 50~100 ㎟ of strain took the most the area (major axis x minor axis). The 5-10mm of stipe length, 2 times of harvesting and volume was 200g~400g took the most. we are selected a middle model through this result. phylogenetic relationships was drawn based on the ITS sequence analysis in Ganoderma lucidum.

This study was carried out to investigate anticancer activities fruiting body extracts and fractions of Cordyceps militaris. Fruiting body of this mushroom was extracted using by 80% MeOH. Fractionations of these extracts were performed by n-hexane, methylene chloride, ethyl acetate, n-BuOH. AGS(human gastric cancer line) was cultured in media conditions (10% FBS, 1% Penicillin-Streptomycin in RPMI). Anticancer activities of each fractions of Cordyceps militaris were examined by using MTT, Cell titer Glo.

국내자생 야생버섯의 항염증 활성검증을 위해 7월~8월에 채집된 버섯을 메탄올 추출하여 DMSO의 희석한 후 Nitric oxide 저해능을 분석하였다. 30여종의 버섯 추출물을 세포활력도를 측정하기 위해 MTT 분석결과 12개의 버섯추출물이 비교적 안정된 세포활력도를 보였으며, nitric oxide 저해능을 양성대조군(LPS)와 비교한 결과 Amanita virgineoides:흰가시광대버섯, Tylopilus neofelleus:제주쓴맛그물버섯, Armillariella tabescens:뽕나무버섯부치, Amanita abrupta:양파광대버섯, Agaricus subrutilescens:진갈색주름버섯, Boletus subvelutips 7종의 버섯은 18%이상의 우수한 nitric oxide저해능을 보였다.

There are an estimated about 20,000 species of mushrooms in the world. Of these, approx. 2,0000 species is edible and around 1,500 species of them grow naturally in Korea. About 300 species of Korean natural mushroom could be classified as edible and medical purpose, but actual use is restricted to only 20 species. Mushrooms has been used for food ingredients since early times. Recently it is being used as a good pharmaceutical material or functional bio-material. Especially Ganoderma lucidum, Phellinus and Inonotus are used for medicinal purposes. In this meaning, the study about chemical component separation and active component effect is necessary. For the study, the systematic establishment of mushroom should be done. In this study, we are trying to build a library by using extracts from the edible, medicinal and wild mushrooms. The physiological activities(antioxidant, anticancer and anti-inflammatory effects) were performed to investigate the effectiveness of mushrooms.

This study was carried out to investigate neuronal protective activity of fruiting body of Hericium erinaceum. In order to search the effective active compound against amyloid beta peptide-induced oxidative stress on neuronal cells, rat pheochromocytoma cells (PC12), Extracts of Hericium erinaceum were screened and evaluated using both the 2’,7’-dichlorofluorescin diacetate assay (DCF-DA), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. According to above assays, Solvent partitions of extracts were selected for further purification and isolation of anti-Alzheimer’s disease compound as it exerted the highest protective effects against hydrogen peroxide (H2O2)-induced oxidative stress.

This study was carried out to investigate characteristic pattern of fruiting body of Ganoderma lucidum and their antioxidant activity. Mycelia of all strains were firstly inoculated into potato dextrose agar(PDA) and then transfered to a media of saw dust which contained 20% rice bran. These mycelia of saw dust were then inoculated into oak tree in polyethylene bags which has been sterilized for 8h at 120℃. The polyethylene bags were sent to a growth room for growth of fruit bodies. Antioxidant activities of each fruiting body were investigated by DPPH method.

This study was carried out to obtain new anti-gout agent from mushrooms. Various extracts from twelve kinds of mushrooms were prepared by water and ethanol extractions and its anti-gout xanthine oxidase inhibitory activity were investigated. Water extract of Flammulina velutipes and Agaricus bisporus fruiting bodies showed high xanthine oxidase inhibitory activity of 70.8% and 60.0%, respectively. However, its inhibitory activity of these ethanol extracts were very low excepet 60.3% of Innotus obliquus. Finally, we selected Flammulina velutipes, showing the highest xanthine oxidase inhibitory activity as a producer of new anti-gout agent.

This study was carried out to investigate characteristic pattern of fruiting body of Ganoderma lucidum and their antioxidant activity. Mycelia of all strains were firstly inoculated into potato dextrose agar(PDA) and then transfered to a media of saw dust which contained 20% rice bran. These mycelia of saw dust were then inoculated into oak tree in polyethylene bags which has been sterilized for 8h at 120℃. The polyethylene bags were sent to a growth room for growth of fruit bodies. Antioxidant activities of each fruiting body were examined by using DPPH(α,α-diphenyl-β-picrylhydrazyl).

This study was carried out to investigate amino acid contents of golden mushroom and pink mushroom. The amino acid analysis was followed by AccQ-Tag method and HPLC on gradient conditions. Seventeen amino acids were analyzed and sixteen amino acids were found in golden mushroom ; fifteen amino acids in pink mushroom respectively. Among total amino acid in golden mushroom, cystein content was the highest and glycine, glutamic acid, proline were followed. Among total amino acid in pink mushroom, cystein was the highest and glycine, lysine, methionine were followed. As shown in Fig.1, 2, concerning amino acids, cystein was dominant. and alanine was detected in golden mushroom and Pleurotus ostreatus sold in market but pink mushroom. Phenylalanine was detected in Pleurotus ostreatus sold in market but that was not detected in golden mushroom and pink mushroom.

This study was carried out to investigate chemical ingredient of Amillaria tabescens. Methanol extract of Amillaria tabescens was analyzed by column chromatography, thin layer chromatography, HPLC, mass spectrophotometer. ATB201 was isolated by prep-HPLC from butyl alcohol fraction. From the physico-chemical data, including NMR, MS, the chemical structure of the compound was determined to be an uracil. (Fig. 1). The molecular weights of uracil was 112.