농산물 재배, 수확 후 세척 등에 사용되는 농업용수는 미생물에 오염될 경우 병원균을 농산물로 전파하여 식중 독을 유발할 수 있기 때문에 관리가 필요하다. 이 연구는 2021년부터 2023년까지 하천수, 저수지, 지하수 등 다양 한 수원의 농업용수에서 대장균군과 대장균의 오염 수준 을 조사하고 자외선(ultraviolet, UV) 소독이 농업용수 내 미생물을 저감시키는 데 얼마나 효과적인지 평가하였다. 농업용수의 미생물 수질조사 결과 대장균 오염도는 수원 에 따라 차이가 있는 것으로 나타났다. 하천수의 대장균 농도는 평균 0.95±1.23 log CFU/100 mL였으며, 저수지는 평균 0.76±1.07 log CFU/100 mL, 지하수는 평균 0.1±0.47 log CFU/100 mL, 기타수원은 평균 0.6±0.87 log CFU/100 mL로 조사되었다. 수원별로 보면 하천수, 저수지, 지하수 순으로 높은 미생물 오염을 보였다. 다양한 용수 조건에 서 자외선 소독을 테스트한 결과, 99.9% 미생물 비활성화 에 필요한 UV 선량은 증류수에서 1.2-1.6 mW/cm2·sec, 농 업용수 원수에서 2.0-2.7 mW/cm2·sec 범위 였다. 고형물 응집제를 통해 전처리된 농업용수는 처리되지 않은 농업 용수 원수에 비해 자외선 투과율이 높았으며, 미생물을 99.9% 감소시키는 데 필요한 자외선 선량이 더 낮아지는 것으로 나타났다. 용수 자외선 투과도가 감소함에 따라 소독 효율이 감소함에도 불구하고 본 실험에서 조사된 선량 은 수질에 상관없이 수초 내 99.9%의 미생물 감소를 달 성하는 것으로 나타나, UV 소독기술은 농업용수의 미생 물 위험을 줄이는 데 효과적인 방법으로 판단된다.

Background: Cadmium (Cd) is toxic heavy metal that accumulates in organisms after passing through their respiratory and digestive tracts. Although several studies have reported the toxic effects of Cd exposure on human health, its role in embryonic development during preimplantation stage remains unclear. We investigated the effects of Cd on porcine embryonic development and elucidated the mechanism. Methods: We cultured parthenogenetic embryos in media treated with 0, 20, 40, or 60 μM Cd for 6 days and evaluated the rates of cleavage and blastocyst formation. To investigate the mechanism of Cd toxicity, we examined intracellular reactive oxygen species (ROS) and glutathione (GSH) levels. Moreover, we examined mitochondrial content, membrane potential, and ROS. Results: Cleavage and blastocyst formation rates began to decrease significantly in the 40 μM Cd group compared with the control. During post-blastulation, development was significantly delayed in the Cd group. Cd exposure significantly decreased cell number and increased apoptosis rate compared with the control. Embryos exposed to Cd had significantly higher ROS and lower GSH levels, as well as lower expression of antioxidant enzymes, compared with the control. Moreover, embryos exposed to Cd exhibited a significant decrease in mitochondrial content, mitochondrial membrane potential, and expression of mitochondrial genes and an increase in mitochondrial ROS compared to the control. Conclusions: We demonstrated that Cd exposure impairs porcine embryonic development by inducing oxidative stress and mitochondrial dysfunction. Our findings provide insights into the toxicity of Cd exposure on mammalian embryonic development and highlight the importance of preventing Cd pollution.

경기도농업기술원에서는 2016년 백색 대형 스탠다드 장미 ‘Fascinar’을 육성하였다. 2011년 ‘Splash’ 품종을 모본으로, 향기가 강한 연보라색 장미 ‘Charming Easies’ 품종을 부본으로 교배하였고 5개월 후에 성숙한 꼬투리를 획득하여 암면배지에 파종하여 후대를 얻었다. 2012년 화색, 화형, 향기 유무 등의 특성검정을 통하여 G11-338 계통을 선발하였다. 선발된 계통을 2013년부터 2016년까지 1차, 2차, 3차 특성검정을 통하여 품종의 안정성과 균일성을 확인하여 ‘Fascinar’로 명명하였고 2017년에 국립종자원에 품종보호출원(제2017-198호) 한 후 2019년에 품종보호권이 등록(제7795호)되었다. 화색은 백색(RHS, N155D)으로 대조품종보다 짙은 백색이며, 화형은 스탠다드이며 고심형인 대형 장미이다. 향기는 대조품종 ‘화이트라임’ 보다 매우 강하며 주요향기 성분은 GERMACRENE-D, Nerol 등 테르펜류와 phenylethyl Alcohol이다. 잎의 형태는 타원형이고 가시가 적은 편이다. 연간 절화수량은 172.2본이고, 꽃잎수는 38.4매이며 절화품질 중 절화장은 75.0cm 절화경경은 6.2mm 절화중은 10.6g 이다. 절화수명은 11일, 화폭 9.2cm, 화고 5.2cm이다. 기존에 육성된 품종에 비해 향기 성분 함량이 높고 화형과 화색이 우수 하여 품종으로서 가치가 높은 품종이다.

Chlorination and UV illumination are being widely applied to inactivate a number of pathogenic microbials in the environment. Here, we evaluated the inactivation efficiency of individual and combined treatments of chlorination and UV under various aqueous conditions. UV dosage was required higher in waste water than in phosphate buffer to achieve the similar disinfecting efficiency. Free chlorine generated by electrolysis of waste water was abundant enough to inactivate microbials. Based on these, hybrid system composed of sequential treatment of electrolysis-mediated chlorination and UV treatment was developed under waste water conditions. Compared to individual treatments, hybrid system inactivated bacteria (i.e., E. coli and S. typhimurium) and viruses (i.e., MS-2 bacteriophage, rotavirus, and norovirus) more efficiently. The hybrid system also mitigated the photo re-pair of UV-driven DNA damages of target bacteria. The combined results suggested the hybrid system would achieve high inactivation efficiency and safety on various pathogenic microbials in wastewater.

The black-veined white, Aporia crataegi (Lepidoptera: Pieridae), which is distributed mainly in Eastern Asia is presumed to be extinct in South Korea, only with some numbers of dried specimens left, whereas the species is found casually in circumferential countries. One of the common conservation practices for such species is to launch introduction program, but prior population genetic analysis between donor and donee populations might be essential for long-term conservation. In this study, we developed 11 microsatellite markers specific to A. crataegi using Illumina paired-end sequencing to investigate the genetic relationships of A. crataegi populations from South Korea and circumferential Asian countries (China, Russia, Mongolia, and Japan). Further, two mitochondrial DNA (mtDNA) gene segments (COI and CytB) were sequenced from the samples. The population- and individual-based Principal Coordinates and STRUCTURE analyses collectively suggested that the South Korean population of A. crataegi is most differentiated from the Japanese population, whereas it was closer to Mongolian and Chinese populations. The STRUCTURE analysis based on two concatenated mtDNA gene sequences also supported different genetic composition of Japanese population from the remaining populations including that of South Korea and rather similar genetic composition between the populations of South Korea and Mongolia. These results collectively suggest that northern populations, in particular, Mongolian populations can be considered as the most genetically compatible one as doner population, when reintroduction program is launched.

Domestic continuously reinforced concrete pavement has been built on a large scale on the Jungbu Expressway, which opened in 1987. Since then, there have been few application due to cost and construction difficulty. Currently, most of the expressway concrete pavements have been applied with jointed plain concrete pavement, but the interest in continuously reinforced concrete pavement has been increasing recently due to frequent premature failure in joints of JPCP. Accordingly, Korea Expressway Corporation has established design guidelines and details for continuously reinforced concrete pavements for expressway in 2014, based on the design method applied to the Jungbu Expressway, but incorporating foreign useful design methods. In terms of construction, pilot constructions were performed on Pyeongtaek~Jecheon Expressway Construction. The new design guidelines were applied to some sections of the Sangju~Yeongdeok Expressway Construction Project in 2016. This study conducted a survey and analysis of transverse cracks for one year after continuously reinforced concrete pavement construction in Sangju~Yeongdeok Expressway. This route was constructed with pavement thickness, base layer and longitudinal reinforcement according to domestic design guidelines. However, the transverse reinforcement was arranged differently according to the section. After construction, the transverse crack spacing was surveyed and the crack gauges were installed at the crack position to measure the movement. This compares the crack spacing according to the characteristics of longitudinal reinforcement and observes continuous movement of the crack width. The results showed that the crack spacing was more affected by the climate at the time of construction than the arrangement method of the transverse reinforcement. The crack width showed periodic changes according to the temperature change over 24 hours. However, due to seasonal changes, it showed a tendency of gradual increasing or decreasing in crack widths, and it was predicted that different measurement results will be shown depending on the time of crack width measurement. Generally, it is predicted that the crack spacing will be narrowed and the crack movement will tend to converge with the period after construction. This initial crack movement data is expected to be useful for comparing whether these predictions are valid.

This study was investigated Quality characteristic of “pickled garlic shoots” manufactured using Vacuum impregnation (VI) and Pressure impregnation (PI) technology. Pickled garlic shoots were manufactured by impregnation solution which concentration were dark soy sauce (39.5 %), apple vinegar (39.5 %), brown sugar (19.8 %), soju (1.2 %). Pressure Impregnation carried out 30 Mpa for 5min or Vacuum Impregnation carried out 360 mmHg for 5min, analyzed treated garlic shoots properties. Impregnation condition were chosen by pre-test (Vacuum Impregnation: 160, 360, 560, 680 mmHg, Pressure Impregnation: 40 MPa, 60 MPa, 80 MPa, 100 MPa). Quality characteristics were pH, Brix, salinity, acidity, color, texture and sensory evaluation. In results of quality characteristics, brix, salinity, sensory evaluation were significantly difference between Pressure Impregnation, Vacuum impregnation and Control. According to sensory evaluation, which parameter were taste, flavor, mouthfeel and overall acceptability, Pressure impregnation were scored highest and Vacuum impregnation were second. In the texture analyze, Hardness of “pickled garlic shoots” which were manufactured using Impregnation technology were 4.3 × 105±784.271 N/m2. This was possible to manufacture “pickled garlic shoots” in a short time which were Universal Design food (UDF) 1 class.

In a previous study, it was reported that enzymatic hydrolysis under pressurization could be a new method which could produce arginine dipeptide and free amino acid in anchovy hydrolysate as salty enhancer at optimal condition. Powder is more efficient than liquid in terms of transport and storage stability. For the purpose of producing spray dried powder of various salt contents was investigated the effect of different salt concentration of anchovy hydrolysate on spray dried powder properties. The anchovy hydrolysate of various salt contents(in the range of approximately 0.7- 19.8% w/w) prepared adding the fish sauce (Dae-Young fish market) at inlet drying air temperatures of 120°C and 140°C. The process yield and physicochemical properties such as moisture content, bulk density, hygroscopicity and the morphology (EDS, XPS, XRD) of the anchovy hydrolysate powder was measured. The glass transition temperatures (Tg) of the powders equilibrated under various water activities were determined using a differential scanning calorimeter (DSC). Different drying conditions and salt concentration could generate anchovy hydrolysate powders with different process yield, bulk density and moisture content. The spray-dried anchovy hydrolysate powder was confirmed by XRD to be a mixture of an amorphous substances and crystalline salts. The energy dispersive X-ray spectrometer (EDS) and X-Ray Photoelectron Spectroscopy (XPS) analysis demonstrated that the surface NaCl concentration of the powders increased with an increasing drying air temperature. Increasing moisture adsorption of the anchovy hydrolysate powders resulted in a Tg reduction. It is suggested that producing spray dried anchovy hydrolysate for the industrial use is the use of the feed salt concentration of not lower than % w/w and inlet air temperature at 120°C, 140°C

목 적 : 가입한 (±)구면렌즈 굴절력에 대응하는 조절력의 변화를 비교하여 렌즈의 적응효과를 알아보았다. 방 법 : 평균연령 21.76±1.76세의 51명(남자 26명, 여자 25명)을 대상으로 하였다. 대상자들의 완전교정 된 눈 앞에 S+1.00 D, S+2.00 D, S+3.00 D, S-1.00 D, S-2.00 D, S-3.00 D를 순서대로 가입하여, 가입 직 후, 15분 후, 그리고 30분 후에 각 단안조절력을 측정하였다. 측정값들은 완전교정 상태의 조절력과 비교 하였고, 렌즈 가입 전·후 측정된 단안조절력의 변화값은 가입한 굴절력 값에 대한 상대 비율로 계산하였다. 결 과 : S+1.00 D, S+2.00 D, S+3.00 D 가입 후 조절력은 완전교정 조절력보다 모두 유의하게 (p<0.001) 증가되었지만, 가입된 렌즈굴절력의 약 55~68% 수준이었다. S-1.00 D, S-2.00 D, S-3.00 D 가입 후 조절력은 완전교정 조절력보다 모두 유의하게(p<0.001) 감소되었고, 가입된 굴절력의 약 72~105% 수준이었다. 결 론 : 조절변화를 목적으로 구면렌즈를 처방할 때, 임상전문가들은 렌즈적응으로 인해 더해준 구면굴절 력 값보다 조절변화량이 대부분 감소될 것이라는 점을 고려해야 한다.

Rubus coreanus is known to have diverse biological properties, such as free radical scavenging activity and antibacterial activity. In the present study, Rubus coreanus leaf and stem extract (RLSE) was used in boar semen preservation whether it has a beneficial effect on assisted reproductive technology (ART) in mammals. Boar spermatozoa were preserved in Beltsville thawing solution (BTS) in the presence of varying concentrations of RLSE (0-10 μg/mL). Sperm motility, sperm viability, and intracellular reactive oxygen species (ROS) levels were examined after 2 days of preservation. The percentage of total motile spermatozoa and progressive motile spermatozoa improved in the spermatozoa preserved with 0.5 μg/mL RLSE. Higher proportions of viable spermatozoa were seen in the presence of 0.5 and 1 μg/mL RLSE than in the control. Intracellular ROS levels decreased when the spermatozoa were preserved in BTS with 0.1–1 μg/mL RLSE. In order to examine the bacterial growth, E. coli was added to liquid semen diluted with antibiotics-free BTS in the presence or absence of RLSE. No anti-bacterial activity of RLSE against E. coli was observed during liquid semen preservation. Although there was no inhibition of E. coli growth, the addition of RLSE might help improve sperm motility and viability during boar semen preservation, suggesting it as a potential reagent for ART in mammals.

The high quality contact between graphene and the metal electrode is a crucial factor in achieving the high performance of graphene transistors. However, there is not sufficient research about contact resistance reduction methods to improve the junction of metal-graphene. In this paper, we propose a new method to decrease the contact resistance between graphene and metal using directly grown graphene over a metal surface. The study found that the grown graphene over copper, as an intermediate layer between the copper and the transferred graphene, reduces contact resistance, and that the adhesion strength between graphene and metal becomes stronger. The results confirmed the contact resistance of the metal-graphene of the proposed structure is nearly half that of the conventional contact structure.

The performance of graphene-based electronic devices is critically affected by the quality of the graphene-metal contact. The understanding of graphene-metal is therefore critical for the successful development of graphene-based electronic devices, especially field-effect-transistors. Here, we provide a review of the peculiar properties of graphene-metal contacts, including work function pinning, the charge transport mechanism, the impact of the process on the contract resistance, and other factors.

The green pale plant bug, Apolygus spinolae was one of the main insect pests that damaged leaves and fruit in grapes and its damage status was firstly reported in 2000 in grape orchards. This research was conducted to evaluate the distribution and difference in damage rate depending in management type of grapevine orchards (domestic sale farm vs export farm) in the export complex area of Korea (Hwangsung in Gyeonggii, Sangju and Yeongcheon in Gyeongbuk, Namwon in Junbuk and Yeongdong in Chungbuk) from 2010 to 2012. Damage by A. spinolae occurred in all 62 survey farms and damage rate differed depending on locality and individual farms in the same area. Damage rate was lower in export farms than in domestic sale farms, and damage rate of leaves was highly correlated with damage rate of new shoots. 15 species of hemipteran insect were attracted to sticky traps and A. spinolae was the dominant species. The attracted number of A. spinolae in the sticky traps differed depending on locality, and more occurred in domestic sale farms than expert farms. A. spinolae was continually attracted to sticky traps in the harvest period in grapevine orchards.