이 연구는 맥류 곡실발효사료 조제를 위한 적정 수확시기 및 수확시기별 곡실 발효사료의 발효품질을 평가하기 위하여 실시하였다. 그 결과, 알곡의 수분함량은 수확시기가 늦어짐에 따라 감소하는 경향을 보였고, 이삭비율과 천립중은 맥종별 모두 출수 후 일수가 경과됨에 따라 높아지는 경향을 보였다. 알곡수량은 맥종 모두 수확시기가 늦어질수록 유의적으로 증가하는 경향을 보였다(p<0.05). 조단백질 함량은 수확시기가 늦어질수록 맥종 모두 높아지는 경향을 보였고, 조섬유함량은 낮아지는 경향을 보였으며, 조지방과 조회분 함량은 비슷하거나 약간 낮아지는 경향을 보였지만 통계적인 유의성은 없었다. 곡실사료의 발효 전후 사료가치를 비교하여 보면, 일반성분들이 발효를 거친 후 약간 증가되는 경향은 있었지만 유의적인 차이는 나타나지 않았다. 발효품질 면에서는 공시한 맥종 모두 수확시기가 늦어질수록 pH는 높아지고, 젖산함량은 유의적으로 낮아지는 경향을 보였다(p<0.05). 초산함량은 맥종 모두 수확시기에 따른 차이를 나타내지 않았고, 낙산은 검출되지 않았다. 따라서 수량과 발효품질을 고려할 때, 맥류 곡실사료 조제를 위해서는 겉보리과 쌀보리는 출수 후 35일, 밀은 출수 후 40~45일에 수확하는 것이 적당한 것으로 판단된다.

남부지역에 비해 상대적으로 사료맥류의 생산이 적은 중 북부지역에서 안정적인 조사료 최대생산과 사료가치 향상 을 위한 최적 혼파조합, 혼파비율, 재배법 확립을 위하여 연 구한 결과 트리티케일과 크림슨클로버 혼파에서는 건물수 량은 트리티케일에 크림슨클로버를 8:2의 비율로 처리한 혼 파조합에서 1,464 kg/10a로 가장 많았으며, 트리티케일이나 크림슨클로버 단파보다 혼파에서 수량이 많았다. 혼파 비율 에 따른 수량 차이는 없었으나 파종유형에 따른 수량차이를 보면 조파보다는 산파에서 수량이 많았다. 트리티케일과 콩과작물의 혼파조합 및 비율에 따른 사료가 치에서 조지방(CP) 함량은 크림슨클로버 단파구가 17.2%로 트 리티케일 단파구의 7.4%에 비해 높았으며, 트리티케일과 크림 슨클로버를 7:3의 비율로 혼파한 시험구에서 조단백질 함량이 9.0%로 개선되어 트리티케일과 콩과 작물의 혼파에 의하여 단 백질을 포함한 사료가치의 향상이 가능할 것으로 생각되었다.

‘조미’는 답리작 적응 내재해 다수성 청보리 품종육성을 목표로 1999년 내재해성으로 총체적성이 우수한 ‘수원337호’를 모본으로 하고, 도복에 강한 ‘수원355호’를 부본으로 교배하여 육성 선발된 ‘익산438호’로 전국 7개소에서 2008년부터 2010년까지 지역적응시험을 거쳐 2010년에 육성되었다. ‘조미’는 직립 초형으로 파성은 Ⅰ정도이고, 잎은 녹색이며, 잎귀가 없는 무엽이 품종으로 가축기호성이 높다. 초장은 90㎝이고, m2당 경수는 846개로 표준품종인 ‘영양’보다 194개 많은 다얼성이며, 엽신 비율이 높다.답리작에서 출수기는 4월 23일로 ‘영양’보다 3일 빠르고, 황숙기는 5월 25일로 1일 빠른 조숙종이다. 내한성은 ‘영양’보다 다소 강하였으며, 흰가루병에는 감수성을 보였지만 보리호위축병에 저항성을 나타냈다. 건물수량은 전작에서 15.7톤/ha ‘영양’과 같았으나 답리작에서 10.3톤/ha으로 ‘영양보다 5% 적었다. 조사료 품질은 조단백질 함량이 8.3%로 ‘영양’에 비해 높았으며, ADF는 26.2%, NDF는 48.3%로 ‘영양’보다 낮았으나 TDN이 68.2%로 ‘영양’보다 다소 높고, 사일리지 등급은 II 등급으로 양호하였다.

‘수양’은 우리나라에서 재배가 가능한 조숙 양질 다수성 쌀귀리 품종을 육성하고자 1996년에 조숙성인 ‘식용귀리’를 모본으로, 조숙 대립 겉귀리 계통인 ‘귀리23호’를 부본으로 인공교배를 하여 계통 및 집단육종법에 의해 선발된 ‘SO96025-B-303-44-2-4’ 계통이다. 이 계통은 2003년부터 생산력검정시험을 거쳐 계통명이 ‘귀리56호’로 명명되었고, 2005년부터 지역적응시험을 거쳐 2010년에 육성된 품종이다. ‘수양’의 잎은 담녹색이고 폭과 길이는 좁고 짧으며, 종실은 길고 황갈색이다. ‘수양’의 출수기는 재배지 평균 4월 28일로서, 기존에 육성된 ‘선양’에 비해 11일 빨랐으며, 성숙기 또한 6월 7일로서 ‘선양’에 비해 6일 빨랐다. ‘수양’의 내한성은 표준품종인 ‘선양’ 보다 강하였고, 도복도 ‘선양’과 같은 수준이었다. ‘수양’은 m2당수수가 568개로 51개 적었으나, 1수립수는 73개로서 7개 많았고, ℓ중은 646 g으로서 596 g인 ‘선양’에 비해 50 g, 천립중은 29.2 g으로 4.2 g 무거운 편이었다. 지역적응시험에서 ‘수양’의 평균 종실 수량은 4.35 톤/ha으로 표준품종인 ‘선양’보다 29% 많았다. ‘수양’은 ‘선양’에 비해 탈부율이 100%로 높고, 조단백질 함량이 11.3%, 베타글루칸 함량이 4.7%로 표준품종인 ‘선양’과 비슷하였다.

‘Muhan’ (Hordeum vulgare L.), a new ruminant-palatability forage barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA in 2013. It was derived from the cross between SB921011-3B-16-2/IT0188556 and ‘Suwon 365’. Among the cross made in 2002, a promising line, SB02T2097-B-B-B-18, showed good characteristics in potential forage yield in the yield trial tested at Iksan in 2009 to 2010 designated as Iksan 465. The line in the Regional Yield Trials(RYT) tested in eight locations around Korea for three years from 2011 to 2013, and was released as the name of ‘Muhan’. It has the growth habit of group Ⅳ, erect plant type, green leaf and awnless spike. Its average heading and maturing dates were on May 4, and June 4, respectively, with similar to check cultivar ‘Youngyang’. The cultivar had 102cm of culm length, 711 spikes per m2 and it showed better rate of leaf, winter hardiness, and resistance to BaYMV than those of the check cultivar. The average forage yield of ‘Muhan’ was about 12.4 ton ha-1 in dry matter in paddy field. ‘Muhan’ also showed 6.6% of crude protein content, 28.6% of ADF (Acid Detergent Fiber), 50.1% of NDF (Neutral Detergent Fiber), and 66.3% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley

‘Jungmo2506’ (Hordeum vulgare L.), a new ruminant-palatability forage barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA in 2013. It was derived from the cross between ‘Sunwoo’ and ‘Milyang 34’. Among the cross made in 2002, a promising line, SB02T2020-B-BB- 64, showed good characteristics in potential forage yield in the yield trial tested at Iksan in 2009 to 2010 designated as Iksan 463. The line in the Regional Yield Trials(RYT) tested in eight locations around Korea for three years from 2011 to 2013, and was released as the name of ‘Jungmo2506’. It has the growth habit of group Ⅳ, erect plant type, green leaf and short awn spike. Its average heading and maturing dates were on May 3, and June 2, respectively, with similar to check cultivar ‘Youngyang’. The cultivar had 99cm of culm length, 692 spikes per m2 and it showed better rate of leaf, winter hardiness, and resistance to BaYMV than those of the check cultivar. The average forage yield of ‘Jungmo2506’ was about 11.8 ton ha-1 in dry matter in paddy field. ‘Jungmo2506’ also showed 6.6% of crude protein content, 27.4% of ADF (Acid Detergent Fiber), 49.2% of NDF (Neutral Detergent Fiber), and 67.2% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley.

Ethephon을 처리하여 트리티케일의 간장을 단축시켜 도복을 방지하는 방법을 찾기 위하여 시험한 결과 Ethephon 처리시 출수기는 수잉기에 처리했을 때는 무처리에 비해 250 ppm과 500 ppm 농도에서는 2일, 1,000 ppm 이상에서는 4일이 늦어졌으나, 개화기와 성숙기는 차이가 없었다. 간장은 Ethephon 농도가 높을수록 작아졌고, 간장 단축률은 수잉기의 1,500 ppm 처리가 37%의 단축률로 처리 효과가 가장 컸으며, 하위절보다 상위절의 단축률이 컸다. 수장, 영화수, 리터중 등에서 무처리와 큰 차이가 없었고, 천립중은 무처리에 비해 약간 무거웠다. 발아율은 차이가 없었으며, 수량은 처리 농도에 관계 없이 모두 증수하는 경향을 보였으며, 수잉기 1,000 ppm 처리에서 최고 5%까지 증수하였다. 발아에 영향을 미치지 않으면서 수확시기를 앞당길 수 있는 방법을 찾고자 실시한 건조제 처리 후 수확시기 단축효과와 종자의 품질을 보면, 건조제 처리후 수확적기 수분함량에 도달하는 일수는 출수 후 30일 처리가 15일, 출수 후 35일 처리가 10일이 걸렸고, 출수 후 40일과 45일 처리가 5일이 걸다. 건조제 처리 후 수확시기는 출수 후 30일과 35일 처리가 관행재배에 비해 8일, 출수 후 40일 처리가 5일이 빨랐다. 건조제 처리시 천립중은 출수 후 처리 일수가 늦어질수록 무거웠고, 발아율 또한 출수 후 처리 일수가 늦어질수록 높아지는 경향을 보였으나, 출수 후 35일 이후에는 통계적인 유의성은 없었다. 수량은 출수 후 30일 처리구가 관행재배의 37% 수준, 출수 후 35일 처리가 70%수준이었고 출수 후 40일 처리가 92% 수준으로 생리적 성숙기 이전에 건조제를 처리하면 품질에 큰 영향을 준다.

This experiment was conducted to obtain basic a information on feed value and fermentative quality of whole crop barley with different harvesting time and making method. As a result, in case of feed value, crude protein, NDF and ADF content were decreased with late harvest, but TDN was increased. In the silage, the results were similar and these patterns showed the same before and after the silage making. Thus, there was no significantly difference in bale and chopper. In case of fermentative quality, pH of the silages showed increasing with late harvesting time but the contents of organic acid did lower, and those tendencies were the same in both bale and chopper condition. But the lactic acid content were approximately 23 percent higher in chopped condition. In conclusion, ensiling at yellow ripe stage is better than other stages, chopped ensiling could improve fermentation quality.

‘Youhan’ (Hordeum vulgare L.), a new whole crop barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA in 2012. ‘Youhan’ has the growth habit of III, light green and middle size leaf, hooded and lax-type spikes. The cultivar showed 107 cm of culm length, 641 spikes per m2. Heading date of ‘Youhan’ was May 1, one day later than that of check cultivar ‘Yuyeon’ in upland, and 2 days earlier than that of check in paddy field. Maturing time was similar to check cultivar ‘Yuyeon’ as June 4 in upland and May 31 in paddy field. ‘Youhan’ also showed better winter hardiness, the resistance to lodging and disease than those of check cultivar. The average forage dry matter yield in the regional yield trial was about 12.6 and 12.0 ton ha-1 in upland and paddy field, respectively, which were 6%, 5% higher than that of the check. It also showed 7.3% of crude protein, 26.8% of ADF(Acid Detergent Fiber), 47.8% of NDF(Neutral Detergent Fiber), and 67.7% of TDN(Total Digestible Nutrients), including higher grade of silage quality for whole crop barley. Fall sowing cropping of ‘Youhan’ is recommended only in areas where average daily minimum mean temperatures in January are higher than －8°C, and it should not be cultivated in mountain areas of Korea.

‘Jungmo2503’ (Hordeum vulgare L.), a new ruminant-palatability forage barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA in 2011. It was derived from the cross between ‘Dongsanpi81’ and ‘Kangbori’. Among the cross made in 1999, a promising line, SB992028-B-B-B-B-B-2, showed good characteristics in potential forage yield in the yield Trial tested at Iksan in 2007 to 2008 designated as Iksan 449. The line in the Regional Yield Trials (RYT) tested in eight locations around Korea for three years from 2009 to 2011, and was released as the name of ‘Jungmo2503’. It has the growth habit of group Ⅰ, erect plant type, green leaf and hood spike. Its average heading and maturing dates were on May 2, and May 29, respectively, with are similar to check cultivar ‘Yuyeon’. The cultivar had 102cm of culm length, 691 spikes per m2 and it showed better rate of leaf, winter hardiness, and resistance to BaYMV than those of the check cultivar. The average forage yield of ‘Jungmo2503’ was about 10.9 ton ha-1 in dry matter in paddy field. ‘Jungmo2503’ also showed 9.4% of crude protein content, 27.3% of ADF (Acid Detergent Fiber), 49.0% of NDF (Neutral Detergent Fiber), and 67.3% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley.

‘Jungmo2502’ (Hordeum vulgare L.), a new ruminant-palatability forage barley cultivar, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, RDA in 2011. It was derived from the cross between ‘Samheung/Suwon 300’ and ‘Milyang 100’. Among the cross made in 2000, a promising line, SB00T2018-B-B-B-B-3, showed good characteristics in potential forage yield in the yield Trial tested at Iksan in 2007 to 2008 designated as Iksan 448. The line in the Regional Yield Trials(RYT) tested in eight locations around Korea for three years from 2009 to 2011, and was released as the name of ‘Jungmo2502’. It has the growth habit of group Ⅵ, erect plant type, green leaf and awnless spike. Its average heading and maturing dates were on May 4, and May 30, respectively, with are similar to check cultivar ‘Youngyang’. The cultivar had 98cm of culm length, 607 spikes per m2 and it showed better rate of leaf, winter hardiness, and resistance to BaYMV than those of the check cultivar. The average forage yield of ‘Jungmo2502’ was about 11.0 ton ha-1 in dry matter in paddy field. ‘Jungmo2502’ also showed 8.7% of crude protein content, 21.9% of ADF (Acid Detergent Fiber), 40.7% of NDF (Neutral Detergent Fiber), and 71.6% of TDN (Total Digestible Nutrients), including higher grade of silage quality for whole crop barley.

’Dahan’ (Avena sativa L.), a winter oat for forage use, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science (NICS), RDA in 2011. It was derived from an original cross between the F1 hybrid of ‘Sprinter’ and ‘73625’ and ‘Gwiri26’ (PA7507-37). Subsequent generations followed by the cross were handled in bulk and pedigree selection programs at Suwon. A line, ‘SO99027-GB-B-113-4-4-3’, was selected for cold tolerance and good agronomic characteristics and designated as a line name of ‘Gwiri75’. The line ‘Gwiri75’ was subsequently evaluated for cold tolerance, earliness, and forage yield in four different locations, Yesan, Iksan, Kimjae, and Jeju, from 2009 to 2011 and finally named as ‘Dahan’. Over 3 years, the average forage dry matter yield of ‘Dahan’ harvested at milk-ripe stage was 15.6 MT ha-1, compared with 14.1 MT ha-1 of check cultivar ‘Samhan’. Though similar in heading date to the check cultivar, ‘Dahan’ had tall plant length and lodging resistance. The feed value of ‘Dahan’ was superior to the check cultivar in percent total digestible nutrients (TDN) and TDN yield per ha. ‘Dahan’ is recommended primarily for winter planting use in the areas where daily minimum mean temperatures are averaged higher than -6 ℃ in January, and excluded in mountain area where frost damage is presumable.

‘Jungmo2502’ awnless barley line (Hordeum vulgare L.) developed at National Institute of Crop Science, RDA in 2011. The Jungmo2502 derived from three-way cross between Millyang100 and F1 (Samheung/Suwon300) in 2000. Subsequent generation were handled by the bulk method in a pedigree selection program. A promising line showed high yield as well as lodging resistance in the yield trial tested at Iksan in 2007 to 2008, designated as Iksan 448. The line in the regional yield trials(RYT) tested in seven locations around Korea for three years from 2009 to 2011. The Jungmo2502 is carrying the growth habit of Ⅳ, green leaf and stem similar to the check cultivar ′Youngyang′. Its heading date was May 3, and maturing time was May 30 in paddy field, which was 3 days later respectively than check cultivar. It was 98 cm of culm length, 607 spikes per m2 and showed more rate of leaf blade, winter hardiness, and resistance to BaYMV than the check cultivar. Average dry matter yield was similar with the check cultivar in paddy field (about 11 ton ha-1). It also showed good qualities as 8.7% of crude protein, 21.9% of ADF (acid detergent fiber), 40.7% of NDF (neutral detergent fiber), and 71.6% of TDN (total digestible nutrients), including high grade of silage for whole crop barley.

The salt adversely affects normal growth and development by the toxic effects of sodium ion (Na+) absorbed from plants. In different plant species, the difference of salt-tolerance degree is related to ion homeostasis, osmolytes biosynthesis, scavenge of harmful operator and water transportation. This intend that salt-tolerance is a quantitative trait controlled by amount of genes. In the previous study, we selected several tolerant cultivars and landraces from over 1,000 barley germplasms which were screened in a high salt (0.4 %, w/w) reclaimed soil. Among the selected germplasms, we selected a salt-tolerant variety from Tunisia (T76) and a salt-susceptible variety Gwandongpi 41 (G41) in germination and early growth stages. In order to develop salt-tolerant segregating population, we performed the development of a new interspecific barley recombinant inbred line population of PB 71 lines deriving from an interspecific cross between Tunisia 76 (T76) and Gwandongpi 41 (G41). The F1 hybrid was progressed by F4 generation. Germination and seedling growth of the F4 generation (a total of 710 lines) were screened for salt-tolerance in 200 mM NaCl solution for 10 days. Ten days after incubation, salt-tolerance was scored on a scale from 1 (sensitive) to 5 (tolerant). In germination screening for evaluation of salt tolerance, the T76 and G41 as parent pants showed all 100 % germination ratio in control or saline conditions. In addition, the whole F4 generations also showed normal germination ratio over 90 % in control condition, while the only 70.42 % of the that showed germination ratio over 90 % in 200 mM NaCl stress condition. The average score of salt tolerance at the seedling stage of parent plants showed that the salt-tolerant cultivar T76 was 5 and the salt-sensitive cultivar G41 was 3 in saline condition. The average score frequency of F4 generations showed that five (7.04 %) among the PB71 lines possessed the highest score 5 and 40 lines possessed the score 3 (26.76 %) and 2 (29.58 %), respectively. This work was supported by grant (KRF-2007-521-F00002) from Korea Research foundation.

In April 2009 two wheat cropping fields, Jeonju and Gimje in Jeonbuk Province, showed yellowish leaves and dead tillers in stem bases with sharp lens-shaped darken lesions. The disease incidence in wheat cultivar Jopummil ranged from 2.2 to 43.5%, with a mean incidence of 28.5%. The different incidence was related the seeding date. Earlier seeding (15th October) fields showed severe incidence as 25.5 to 43.5% while late seeding around the end of October weakened incidence in the cultivar. Based on morphology and pathogenic characteristics, the fungus was identified as Rhizoctonia cerealis that causes sharp eyespot in wheat. The disease severely affected growth and yield including culm length, spike length, number of kernel. The average culm length of the infected cv. Jopummil was 66 cm while that of healthy plant was 74 cm. The number of kernel and tiller per unit area was decreased only in the case of more than 13% incidence, but the length of heads was not significantly different compared with that of the healthy plants.

Barley landraces harbor significantly higher genetic diversity than modern cultivars. Diversity of agronomic and morphological traits was investigated for 386 accessions of Korean landrace barley which were back-introduced from Okayama University, Japan. Among the accessions, 279 were covered barley and 89 naked barley. Seeds were sowed in late October and major growth characteristics were investigated at maturity and after harvest. The traits evaluated exhibited higher variation with respect to the CV, ranging from 47 to 60. The trait exhibiting the greatest variation was flag leaf width (FLW, CV=60 with 2.8x difference between minimum 0.6 and maximum 1.7). Those traits exhibiting least variation were heading date (HD, CV=47.2 with 31 days difference between the earliest and the latest) and seed number per spike (SNS, CV= 48.9 with 2.8x difference between minimum 36 and maximum 72). The other eight traits [maturity date (MD, CV= 53.7), culm length (CL, CV= 55.4), flag leaf length (FLL, CV= 51.6), spike length (SL, CV= 54.3), awn length (AL, CV= 54.4), whole spike length (WSL, CV= 52.9), spike density (SD, CV= 52.7), seed rows of spike (SRS, CV= 50.1)] were intermediate in their level of variation with respect to the CV. The first, second and third principal components explained about 30.3%, 17.0% and 13.4% of the total variation of the traits, respectively. The traits which contributed more positively to PC1 were SL, SD, WSL and AL. The traits which contributed more positively to PC2 were SRS and SNS, and those contributed more positively to PC3 were FLL and FLW. The Korean landrace barley accessions contain significantly diverse variation to explore for the improvement of agronomic and morphological traits of current elite cultivars. This work was supported by a grant from Regional Subgenebank Support Program of Rural Development Administration, Republic of Korea.

　A new naked oat cultivar ‘hoyang’(Avena sativa L.) was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2007. It was derived from a cross between ‘ikyonggwiri’and ‘wiri23’ The cultivar, Sikyonggwiri is early heading while the breeding line Gwiri23 has a high yielding potential with large-size grain. Bulk method combined with pedigree selection program was employed in subsequent generations, and the promising line SO96025-B-303- 44-2-5 was selected for agronomic performance in 2002. The line showed both high yield and good husking rate of seed in the Yield Trial tested at Suwon in 2003 to 2004, and was subsequently designated as ‘wiri57’ Gwiri57 was evaluated for winter hardiness, earliness, and yield in four locations, Gimje, Iksan, Jeongeup and Jinju from 2005 to 2007 and was released as Choyang. Choyang headed 11 days earlier and matured 5 days earlier than the check cultivar ‘unyang’in the paddy field condition. The new cultivar Choyang had 97 cm of culm length and 23.4 cm of spike length, 658 spikes per m2, 75 grains per spike, 28.8 g of 1,000-grain weight, and 636 g of test weight. Choyang showed better winter hardiness than that of the check cultivar Sunyang, and similar seed quality to the check cultivar in respect to crude protein (12.9%) and β-glucan content (4.7%). However, it showed higher husking rate than the check cultivar. The grain yield of Choyang in the regional yield trial for 3 consecutive years was averaged 4.67 MT ha-1, which was 38% higher than that of the check cultivar Sunyang. Choyang is recommended for the fall sowing cropping only in the southern area where daily minimum mean temperatures are averaged higher than -4℃ in January, and is not recommended the in mountain area where frost damage is presumable.

Major agronomic and morphological traits of Korean land races of cultivated barley were analyzed. Three hundred sixty-three Korean land races, 69 hull-less and 295 hulled barely, were cultivated at the field of National Honam Agricultural Research Institute at Iksan, Korea. Olbori and Saessalbori were used as control varieties for hulled and hull-less barley, respectively. Traits such as heading date, maturity, plant height and flag leaf length and width were analyzed. Heading date and maturity were variable 22 and 22 days, respectively, among the land races tested. Plant height of land races corresponded to 74 to 174% of that of the control varieties. Flag leaf length and width varied from 87% to 314% and from 57 to 233%, respectively, of that of the control varieties. This result indicates that genetic diversity for agronomic and morphological traits are significantly broad among Korean land races and they could provide valuable genes to improve current elite lines.

The objectives of this study were to collect and maintain wild barley accessions and to analyze their phylogenetic relationships among the accessions. Sixty wild barley accessions were collected from Syria (four accessions), Jordan (eight), Afghanistan (six), Iraq (three), Iran (four), Turkey (two), Pakistan (one), Tajikistan (one), Azerbaijan (one), Palestine (fifteen), and unknown area (fifteen). Seeds of the three accessions failed to germinate. Thirty-seven random amplified polymorphic DNA (RAPD) were generated and the phylogenetic relationship of the accessions was analyzed. Large genetic diversity existed among the collections and the collections were classified into 4 groups at a similarity coefficient of about 0.7698. Significant variations were observed within the collections from the same geographical location. The collections have sufficient genetic and morphological diversity for the genetic and phenotyphic analyses of traits associated with tolerance to abiotic stresses such as salt and drought.

Thirty-nine out of eighty-five barley varieties/strains survived until heading stage in the saline experimental field (0.03-0.05~%~;salt) and they were used for pollen study. Light and scanning electron microscopic observations revealed two distinctive types of barley pollens: one transparent and small in size and the other dark and larger. In addition, both types of pollens were stained with Alexander's stain and it was found that the smaller and transparent pollen was cytoplasm-devoid (CD) while the larger pollen was cytoplasm-rich (CR). Sixteen out of 39 barley varieties/lines grown in the saline soil had CR pollens, which were rarely observed in the barley plants grown in the non-saline soil. Moreover, it was observed that salt stress severely reduced seed setting in the varieties having degenerated pollens. These results suggest that salt stress affects the fertility of barley pollen. The sterile pollen was undersized and lack of cytoplasm probably due to abortion. Furthermore, a varietal difference existed in the response of pollen development to salt stress.