In this study, emission characteristics of odorants from boiled egg samples were measured through time between fresh and decayed status. Air samples released from boiled egg were collected and analyzed five different periods during the whole experimental period set for 9 days. The concentrations of reduced sulfur compounds (RSC), carbonyl compounds (CC), and NH₃ were generally higher than others like volatile organic compounds over the entire period. Among target compounds, the concentration of H₂S recorded the maximum value of 3.65 ppm immediately after boiling which indicates its maximum contribution as odorant in fresh stage. In addition, some odorants (i.e. CH₃SH, AA, PA, BA, and VA) were detected in the range of 7.52 to 50.8 ppb. Emission of most odorants generally decreased after 1 day of boiling, although their concentrations increased again after 3 days to reach the peak values after 6 days. The dilution-to-threshold (D/T) ratio measured by air dilution sensory test samples between fresh and decayed egg showed patterns similar to H2S. In addition, the result of correlation analysis indicated that there is a strong relationship (p＜0.01) between complex odor and H₂S.

This survey focused on consumer's consumption patterns for buying factors of domestic live stock products after foot and mouth disease (FMD). The purpose of this survey was to suggest basic information to reduce consumption when livestock diseases are occurred. Two methods were used for this survey. First method was to investigate internal data to understand consumption patterns when buying domestic livestock products. Second method included empirical analysis to understand how FMD affected consumer's consumption pattern. Survey was conducted in Chuncheon city. As the result of this survey, factors that affected the buying patterns of domestic livestock products were brand and expiration date. These results suggested that consumers recognized brand livestock products were safer than general livestock products and they have more interest for food safety than quality attributes of food when buying domestic livestock products.

The present study examined the expression of porcine sirtuin 1–3 (Sirt1–3) genes in immature (germinal vesicle; GV stage), mature (metaphase II; MII stage) oocytes, preimplantation embryos derived from parthenogenetic activation (PA), in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT). We also investigated the role of sirtuins in oocyte nuclear and cytoplasmic maturation, and embryonic development of PA and IVF embryos using sirtuin inhibitor [5 mM nicotinamide (NAM) and 100 μM sirtinol]. The expression of Sirt1–3 mRNA was significantly (p<0.05) up-regulated during IVM. The expression patterns of Sirt1–3 mRNA in preimplantation embryos of PA, IVF and SCNT were gradually (p<0.05) decreased from MII stage of oocyte to blastocyst stage. Especially, the expressions of Sirt1 and Sirt3 in SCNT blastocysts were significantly lower than IVF blastocysts. Treatment with nicotinamide (NAM) during IVM resulted in significantly decreased nuclear maturation but it was restored when NAM treated with 2 μM resveratrol (RES; known as antioxidant and sirtuin activator) compared to the control (control: 88.9%, NAM: 67.9% and NAM+RES: 86.4% respectively). Intracellular reactive oxygen species (ROS) level of oocytes matured with NAM was significantly increased and with NAM+RES was significantly decreased compared to the control. Treatment with sirtuin inhibitors during IVC resulted in significantly decreased blastocyst formation and total cell number of blastocyst derived from PA (NAM: 29.4% and 29.6, sirtinol: 31.0% and 30.3, and control: 40.9% and 41.7, respectively) and IVF embryos (NAM: 10.4% and 30.9, sirtinol: 6.3% and 30.5, and control: 16.7% and 42.8, respectively). There was no significant difference in cleavage rate both PA and IVF embryos. Oocytes treated with NAM during IVM showed significantly lower expression of PCNA, Bax, Bcl-2, POU5F1 and Sirt1–3 compared to the control. Oocytes treated with NAM+RES during IVM restored gene expression except POU5F1. Similarly, PA derived blastocysts treated with NAM during IVM showed down-regulation of PCNA, Bax, Bcl–2, POU5F1 and Sirt1–2. The blastocysts derived from PA embryos treated with sirtuin inhibitors during IVC showed lower (p<0.05) expressions of POU5F1 and Cdx2 genes. Also, Sirt2 mRNA expression was significantly decreased in sirtinol treated group and Sirt3 mRNA expression was also significantly de -creased in both NAM and sirtinol treated groups compared to the control. These findings indicate that Sirt1–3 which are transcribed and stored during oocyte maturation may have physiological and important roles in porcine oocyte maturation and preimplantation embryonic development by regulating gene expressions. * This work was supported by a grant from Next-Generation BioGreen 21 program (# PJ008121), Rural Development Administration, Republic of Korea.

Palatal development is one of the crucial events in craniofacial morphogenesis, according to the significant signaling pathway including the out growth, elevation, and fusion of palatal shelves. In the fusion of palatal shelves, epithelial to mesenchymal transition (EMT) is a fundamental process to achieve the proper morphogenesis of palate. Mechanisms of EMT have been reported as the processes of migration, apoptosis or general EMT through the modulations through various signalling molecules. Rgs19, known as a regulator of G protein signaling (RGS) family through GTPase activity, showed the interesting epithelial expression patterns in various organogeneses including the significant expression patterns of Rgs19 in palatal development. To evaluate the precise function of Rgs19 in palatogenesis, we employed the gain and loss of function studies using ASODN treatments and gene electroporations while in vitro palate organ cultivations. Knockdown of Rgs19 using treatments of AS-ODN showed the retarded palatal fusion with the decreased patterns of apoptosis in mesial epithelium edge (MEE). In addition, alteration patterns of related genes were examined with the qRT-PCR. And epithelial mesenchyme transition (EMT) process was delayed in medial edge epithelium (MEE) throught immunohistochemistry of pancytokeratin, which known as epithelial cell marker. Morphological changes were observed with the three dimensional reconstruction method. These results show that expression of Rgs19 in MEE has crucial role of EMT, also Rgs19 affects to palatal fusion by regulation of apoptosis through the signalling modulations.

To determine current rate of antimicrobial resistance, a total of 236 isolates from milk samples of dairy cattle with mastitis in Korea during 2010-2011 were examined against 12 antimicrobials using disc diffusion method: 67 Staphylococcus aureus, 74 coagulase-negative Staphylococcus spp. (CNS), and 95 Escherichia coli isolates. The isolates examined in this study were submitted by Local Veterinary Service Laboratories located in 13 provinces and metropolitan cities nationwide. The highest rates of resistance among S. aureus isolates were against ampicillin (56.7%) and penicillin (56.7%), followed by kanamycin (11.9%). All S. aureus isolates were sensitive to lincomycin, amikacin, and cephalothin. Only one isolate showed resistance to tetracycline and oxacillin, respectively. Less than 10% of the S. aureus isolates presented resistance to erythromycin, neomycin, and gentamicin. Among CNS isolates, the most frequently observed resistance was to lincomycin (44.5%), followed by penicillin (28.3%), ampicillin (18.9%), tetracycline (17.5%), kanamycin (13.5%), and erythromycin (9.4%). All or most of the CNS isolates were sensitive to cephalothin, amikacin, neomycin, and gentamicin. The highest rate of resistance among E. coli isolates was against tetracycline (26.3%), followed by streptomycin (21%), neomycin (15%), kanamycin (12.6%), and gentamicin (10.5%). Amikacin was the only antimicrobial to which no E. coli isolates showed resistance. Around 10% of the S. aureus isolates and 15% of the CNS isolates showed resistance against three or more antimicrobials simultaneously, while more than 30% of the E. coli isolates did.

This study examines the dietary, exercise, and other daily habits of Daegu residents and how these relate to the residents’ perception of their own health status, and comparatively analyzes the lifestyles and daily habits of those who perceive themselves to be healthy and those who do not. This research study used Inbody 230(Body composition analysis, Biospace, Korea) to make body and health measurements such as body composition and obesity index. Also, included in this study was a survey on the lifestyle patterns of the residents. The data from this survey was analyzed with SPSS. The results show that among the residents of Daegu, those who perceive themselves to be healthy have lower body fat and are less obese on average. With respect to dietary habits, those who believe themselves to be healthy have more regular dietary habits such as rarely skipping a meal than those who do not believe themselves to be healthy. In addition those who say they are healthy exercise more frequently and for a longer duration than those who say they are not healthy. As for daily habits, those who report they are healthy show greater satisfaction with life and suffer less from stress than those who report themselves to be unhealthy. This study demonstrates that in order to improve people’s health in Korea, good dietary, exercise and daily habits need to be emphasized. Additionally, health education and health awareness programs need to be established in each region. Follow-up studies should be conducted afterwards.

목 적: 취학 전 어린이와 부모의 시력검사 행동양식을 조사하여 취학 전 어린이의 시력검사 참여율을 높 이기 위한 개선방안을 제안하고자 한다. 방 법: 대전지역 12개 유치원의 만6세(평균연령 6.0±0.2세) 어린이 487명을 대상으로 시력검사와 자동 굴절검사, 자각식 굴절검사, 가림검사, 허쉬버그검사를 실시하였다. 설문지를 이용하여 유치원 어린이와 부 모의 시력검사 행동양식을 분석하였다. 결 과: 조사대상자들의 등가구면굴절력은 오른쪽 눈 -0.15±0.81D, 왼쪽 눈 -0.14±0.75D였다. 조사대 상 어린이 487명 중 73명(15.0%)이 비정시이었고 이들 중에서 5명(6.8%)이 안경을 착용하고 있었다. 취학 전 어린이에서 189명(42.4%)이 시력검사 경험이 있었으며 이들 중에서 56.1%(n=106)가 집단시력검사 기회 를 이용하였다. 부모들에서는 54.8%(부 57.9%, 모 51.6%)가 정기적으로 시력검사를 받고 있었고 이들의 51.6%(부 55.8%, 모 46.6%)가 집단시력검사 기회를 이용하고 있었다. 취학 전 어린이와 그들의 부모의 시 력검사 행동양식은 비슷한 경향을 보였다. 결 론: 취학 전 어린이의 시력검사는 제도화된 집단검사를 통해 시행되어야 하며 참여율을 높이고 검사 결과의 신뢰성을 높이기 위해 시력검사 전문가인 안경사를 활용하는 방안을 제안한다.

This study considered the whole intake patterns and realities of consumption of instant noodles on sales targeting children and adolescents. Based on a total of 1021 subjects, data from self-administrated questionnaires were collected and analyzed using comparative statistical analysis, including frequency, chi-square, t-test, and one-way ANOVA. Results of the study were as follows. The preference levels for instant noodles by children and adolescents were 5.25, thereby showing a more than moderate preference. Middle school boy students' preference for instant noodles was the highest. The main reason for preferring instant noodles was taste and convenience. The main reason for disliking instant noodles was poor nutrition and likelihood of becoming fat. The intake of instant noodles was the highest between lunch and dinner with a frequency of once to twice per week. Content of soup powder given taking instant noodles was the largest in having putted all. The soup quantity of being left given taking instant noodles was possessed the majority in almost not eating soup. The main reason for not consuming soup was being full or concerns for health. A significant difference was observed in preference level for instant noodles and in intake patterns depending on the respondents' general characteristics. Limitations and future research directions are also discussed.

Polydimethylsiloxane(PDMS) 몰드를 사용한여소프트 리소그래피 방법을 통해 Poly(3-hexylthiophene)(P3HT)가 서브 파장(subwavelength) 사이즈로 나노패턴 된 전기 변색 소자를 제작하였다. 나노 패턴된 전기 변색 소자를 사용한여 착색 및 소색 상태에 따른 빛의 편광 효과를 패턴의 방향을 바꿔가며 측정하였으며, 인가전압에 따라 변화되는 고분자의 도핑 상태에 의해서 편광된 빛의 세기를 손쉽게 가역적으로 변화시킬 수 있었다. 편광 효율을 최댓값과 최솟값의 비로 정의하여 실험을 통해 1.7의 편광 효율값을 계산할 수 있었다. 산화, 환원 반응에 따른 고분자의 구조변화에 의해 도핑 상태에 따라 굴절률 차이가 생기게 되고 이에 따라 편광 된 빛의 세기를 인가전압의 스위칭만으로 조절할 수 있었다. 전기 변색 소자에 -2V와 2V 전압을 인가해 주었을 때 고분자 패턴과 전해질 사이의 굴절률 차이는 0.61과 0.99의 값으로 나타났다.

The purpose of this study was to develop slacks patterns for middle-aged abdomen-obese adult males by using the 3D virtual-twin and virtual-garment simulation system. The criteria for subjects in this study were males who had over 25kg/m2 of BMI, over 90cm of waist, and over 0.90 of WHR. A total of 211 adult males who met these criteria were enrolled. The results were as follows: first, a new slacks pattern considerate of abdomen-obese men was development. The basic numerical formula were as follows: front and back hip girth H/4+3.5, front waist girth W/4+1+0.5, back waist girth W/4+1－0.5, front crotch extension H/16, back crotch extension H/8－0.5, front pleats amount 2.7, and back dart amount 1.5. Second, according to the results of the new slacks patterns appearance evaluation, the new slacks pattern scored more highly than the existing pattern in silhouette and ease amount, confirming that the new slacks pattern is appropriate for the abdomen-obese men. Also, the new slacks pattern was evaluated allowing proper space length of waist, abdomen and hip. Virtual models of production through data from a 3D body scan, pattern draft and virtual garment digital program were applied to a prototypic design method in order to enhance the fitness of ready-made garments. The use of the virtual twin made it impossible to comprehend the appearances and ease correspondent to motions. In order to evaluate wearing fitness, therefore, the system should be improved so as to change arm positions and perform various motions.

Traditional culture could be a wonderful source of inspiration for creative design. Tteoksal, the stamps used for making decorative patterns on traditional rice-cakes, have been used and passed on to generations to show the emotions of our ancestors. Numerous traditional patterns found in Tteoksal can be used to better understand and transmit tradition in modern ways. The purpose of this study was to develop fashion cultural products with a modern sensibility by understanding the origin, types and characteristics of Tteoksal and its patterns. The method of this study was through considering the proceeding studies and the references published by a specialist and museums having some information about further knowledge on Tteoksal. In line with the aforementioned study, neckties, eco-friendly bags and pouches were developed as fashion goods. Among the Tteoksal's patterns selected were lotus, bats, 樂letters and complex patterns. Using a computer graphic software program such as Adobe Illustrator CS5, archetypes of patterns were traced as vector graphics, and two design motives were developed in every pattern. The patterns were developed into two types: one was a basic pattern faithful to a basic motive pattern, and the other was a varied type that was changed and applied. The fashion products were developed as two styles for basic and varied patterns for neckties, eco-friendly bags and pouch items. Twelve products were made for each pattern, which totals 48 unique products. In conclusion, the study could be a critical step to better understand the traditional culture and its influence to the patterns applied to modern fashion design.

The purpose of this study was to investigate the snack and beverage intake patterns of students by body mass index groups (BMI 〈18.5, 18.5-23, 23-30, ≥30). Questionnaires were completed by 1381 high school students in Seoul, Incheon, and Gyeonggi-do, the area of Korea's capital region. There were no significant differences in skipping meals for overweight (BMI 23-30 and ≥30) or under-nourished (BMI 〈18.5) students. Girls skipped dinner more frequently than boys. Boys and girls both preferred meat and disliked fish regardless of BMI. Girls with BMI 23-30 disliked vegetables. Boys and girls would rather have crackers, candies, and chocolates than potatoes as snacks regardless of BMI. Obese boys (BMI ≥30) preferred flour-based food, fast food, and other food as snacks. Girls liked fruits more than boys. Snacks were eaten 2-3 times per week, when students were hungry or bored. There were no significant differences in the frequency or reasons (habitual, stressed) for snack consumption by BMI. Girls liked juice more than boys did, and boys preferred soda water more than girls did. Both the under-nourished (BMI 〈18.5) boy and girl groups had more juice than the overweight (BMI 23-30 and ≥30) groups. Obese (BMI ≥30) boys did not more drink soda water than other BMI groups. The under-nourished (BMI 〈18.5) boy group had more soda water than the normal (BMI 18.5-23) and overweight (BMI 23-30) groups. Girls in the overweight (BMI 23-30) group had 2 more cups of soda water a day than the normal group (BMI 18.5-23). Therefore, skipping meals and beverage intake patterns might influence BMI.

Palatal development is one of the crucial events in craniofacial morphogenesis, according to the significant signaling pathway. In the fusion of palatal shelves, EMT is a fundamental process to achieve the proper morphogenesis of palate. Mechanisms of EMT have been reported as the processes of migration, apoptosis or general EMT through the modulations through various signalling molecules. Rgs19, known as a RGS family through GTPase activity, showed the interesting epithelial expression patterns in various organogenesis including the significant expression patterns of Rgs19 in palatal development. To evaluate the precise function of Rgs19 in palatogenesis, we employed the loss of function studies using AS-ODN treatments while in vitro palate organ cultivations. Knock-down of Rgs19 using treatments of AS-ODN showed the retarded palatal fusion with the decreased patterns of apoptosis in mesial epithelium edge (MEE). In addition, alteration patterns of related genes were examined with the qRT-PCR. And EMT process was delayed in MEE throught staining of pancytokeratin, which known as epithelial cell marker. These results show that expression of Rgs19 in MEE has crucial role of EMT, also Rgs19 affects to palatal fusion by regulation of apoptosis through the signalling modulations. Palatal development is one of the crucial events in craniofacial morphogenesis, according to the significant signaling pathway. In the fusion of palatal shelves, EMT is a fundamental process to achieve the proper morphogenesis of palate. Mechanisms of EMT have been reported as the processes of migration, apoptosis or general EMT through the modulations through various signalling molecules. Rgs19, known as a RGS family through GTPase activity, showed the interesting epithelial expression patterns in various organogenesis including the significant expression patterns of Rgs19 in palatal development. To evaluate the precise function of Rgs19 in palatogenesis, we employed the loss of function studies using AS-ODN treatments while in vitro palate organ cultivations. Knock-down of Rgs19 using treatments of AS-ODN showed the retarded palatal fusion with the decreased patterns of apoptosis in mesial epithelium edge (MEE). In addition, alteration patterns of related genes were examined with the qRT-PCR. And EMT process was delayed in MEE throught staining of pancytokeratin, which known as epithelial cell marker. These results show that expression of Rgs19 in MEE has crucial role of EMT, also Rgs19 affects to palatal fusion by regulation of apoptosis through the signalling modulations.

The aim of this study was to evaluate the changes of protein patterns in granulosa cells and corpus luteum during the estrus cycle in bovine ovary by proteomics ^techniques. Our study was devided into five steps for follicular, ovulatory, early-lteal, midluteal and late-luteal. The protein was extracted from glanulosa cell and corpus luteum proteins by using M-PER Mammalian Protein Extraction Reagent. Proteins were refined by clean-up kit and quantified by Bradford method until total protein was 700 μg. Immobilized pH gradient (IPG) strip was used 18 cm and 3 11 NL. SDS-PAGE was used 10% acrylamide gel. The protein spots were visualized by Coomassie Brilliant Blue (CBB) staining, analyzed by MALDI mass spectrometry and searched on NCIBlnr. As the result, 61 spots of total 85 spots were repeated on follicular stage and 51 spots of total 114 spots were repeated on ovulatory stage. 40 spots of total 129 were repeated on early-luteal and 49 spots of total 104 spots were repeated on mid-luteal stage. Also 41 spots of total 60 spots were repeated on last-luteal stage. There were differences in the ovulation (follicular∼ovultory stage) in which the spots of follicular stage 19 was only and in ovulation stage was 10 spots. The difference between the luteinization (ovultory∼mid-luteal stage) was the spots counted in each stage. The spots of ovulatory stage was 1, early-luteal stage was 1 and in mid-luteal stage was 2. Eleven spots were found in mid-luteal stage and 2 spots were found in last-luteal stage. In conclusion, we confirmed that there were 7 spots in ovulation, 4 spots in luteinization and 2 spots in luteolysis. Spot No. 89-93 from ovulation were transferrin, and spot No.94 and 95 were HSP60. Spot No. 103 were Dusty PK, spot No. 135 were OGDC-E2, and spot No. 175, 176 were Rab GDI beta from luteinization. Spot No. 178 and 179 from luteolysis were vimentin.

It was conducted the experiment, divided into three groups as normal, poor and polycystic ovary syndrome, to detect the change of protein patterns in follicular fluid on ovarian response following controlled ovarian hyperstimulation for human IVF outcome. In the normal group, it was confirmed reproducible 57 spots in the detected total 81 spots. Then 1 spot was not found in the other groups. In the poor responder group, it was found reproducible 53 spots in the detected total 98 spots. 6 spots were down-regulation and 7 spots were up-regulation comparable with normal group. There were not 5 spots in poor responder group comparable with other groups. In the polycystic ovary syndrome group, it was expressed reproducible 53 spots in the detected total 80 spots and 3 spots were just expressed in this group. However, 4 spots were not found in polycystic ovary syndrome. 9 spots were up-regulation comparable with normal group. Significant up and down-regulation spots among the each groups were identified. The results were a cytosolic carboxypeptidase, a signal-induced proliferation-associated protein 1, a ceruloplasmin, a keratin(type Ⅱ cytoskeletal 1), a polypeptide N-acetylgalactosaminyltransferase 2, a serine/threonine-protein phosphatase 4 regulatory subunit 4. It was identified that 8 spots, 6 kinds of protein are corresponded with NCBInr database research, but 10 spots were failed in the identification. In conclusion, it has been confirmed change and expression of protein on the ovarian response following COH of human.

This study was carried out to investigate antibiotic resistances of 181 isolates in Staphylococcus aureus isolated from 450 specimens which were a General Hospital personnel in Cheonan province. Of the 181 isolates, 89 isolates (49.2%) were methicillin resistant and 92 isolates were susceptible. They were resistant to penicillin (80.1%), clindamycin (18.8%), oxacillin (49.2%), cefepime (28.2%), cefotetan (34.3%), ampicillin (66.3%), gentamicin (27.6%), tetracycline (17.7%) and erythromycine (43.1%), also. In resistant rates of the 89 MRSA, 5 antibiotics were more than 50%, but in those of the 92 MSSA, only 1 antibiotics was. Also they showed very highly multi-drug resistance patterns to 15 antibiotics as follow : One hundred and forty five isolates(82.3%) showed antibiotic resistance to more than one kind of the 15 kinds antibiotics. In the multiple resistant patterns, one drug resistant isolates were 18 isolates (9.9%), 2 drugs 29 (16.0%), 3 drugs 21 (11.6%), 4 drugs 29 (16.0%), and more than 5 drugs were 52 isolates (28.7%). The number of more than 5 drugs resistant strains in MSSA were only 1 strain, but those in MRSA were 51 isolates (57.3%). Forty five isolates (24.9%) produced enterotoxin serotype C and all MRSA.

형식주의비평은 예이츠 연구에서 비교적 주변적 역할을 하고 있다. 최고의 시인이라는 명성이 기교적 탁월함에 근거한다는 일반적 인식을 고려할 때, 이것은 좀 놀랍다. 최근에, 그러나, 벤들러의 선도적 연구는 이 비평적 공백을 메우기 시작했다. 이 글은 예이츠 시에서 구체적으로 서정시 장르와 준서정시로 구분하는 벤드러를 논의한다. 본 논문은, 소네트 형식의 5편의 시에서, 마지막 두 행의 변화(turn)와 수욕적 힘의 대두 사이에 결합이 발생한다는 것을 보여준다. 이 관찰은 준서정시의 구분의 타당성에 의문을 제기하며, 결론적으로 장르구분의 확인의 한계를 지적한다.

This study was performed to investigate patterns of fortified food (FF) consumption and intake of vitamins and minerals from FFs among 577 Korean children (12.4 years of age) who attended elementary or middle school. FFs eaten by children as a snack were surveyed using the food record method during 3 days, including 2 week days and one weekend. As a result, 114 FF items were eaten by the children, and several kinds of nutrients such as vitamin A, D, E, B complex, C, calcium (Ca), iron (Fe), and zinc (Zn) were fortified in these foods. Ca-FFs (65.8%) were most frequently consumed, followed by vitamin C-FFs (33.4%) and vitamin D-FFs (33.3%). The number of FF items in each food group was the most in the milk group (n=24, 21.0%), followed by the beverage group (n=19, 16.7%), and the cookie/bread/cake group (n=17, 14.9%). Fortified nutrients in FFs were in various combinations, but the major combination patterns were Ca, Ca plus vitamins, Ca plus vitamins plus other minerals, and Ca plus other minerals. Daily mean intakes of vitamins and minerals from the FFs were 66-300% more than those of the recommended nutrient intake (RNI ) or adequate intake (AI) for most vitamins and minerals. Daily maximum intakes (95th percentile) of vitamins and minerals from FFs were 1-15 times the RNI or AI for most vitamins and minerals. Vitamin and mineral consumption ratios from each FF group were different according to the kind of fortified nutrient. For example, vitamin C was mostly eaten in fortified beverages (46-54%), and Fe was mostly eaten in fortified cookie/breads/cakes (87%). The above results show that FF consumption varied widely among the children, and that most of the children's foods were fortified with several vitamins and minerals without a common rule; thus, subjects risked over consuming vitamins and minerals by eating FFs. Therefore, practical guideline on FF use for children's optimal nutrition and health should be provided through nutrition education.