Changes in ATP and related compounds, TMAO, TMA, creatine and creatinine were analyzed to establish the processing conditions for rapid- and low salt-fermented liquefaction of anchovy(Engrulis japonica) extracts during fermentation. Experimental sample A: chopped whole anchovy, adding 20% water, heating at 50℃ for 9 hrs and then adding 10% NaCl. Sample B: chopped whole anchovy, adding 20% water, heating at 50℃ for 9 hrs and then adding 13% NaCl. Sample C: chopped whole anchovy adding 13% NaCl. Sample D: whole anchovy adding 17% NaCl. ATP, ADP, AMP and IMP were broken down during fermentation period, while inosine and hypoxanthine or hypoxanthine were detected in each fermented liquefaction of anchovy. However the amounts of them were varied from collection to collection according to the pretreatment methods. Possibly ATP and their related compounds will not make a great contribution to the umami taste in fermented liquefaction of anchovy. The contents of TMAO were decreased during fermentation period, ranging from 3 to 15 mg/100g in the fermented liquefaction of anchovy after 180 days. The TMA contents were increased slowly during fermentation period, ranging from 60 to 114 mg/100g in the 180 days specimens, however their contents were varied from sample to sample. The contents of creatine and creatinine were increased during early fermentation period, and then they were decreased in the last period. As for distribution of nitrogen in the anchovy extracts, the contribution of creatine and creatinine to the extractive nitrogen was occupying 6.8, 5.7, 4.6 and 5.7% in the experimental sample A, B, C and D, respectively. The contribution of ATP and related compounds to the extractive nitrogen was occupying 2.1, 2.4, 2.2 and 2.7% in the experimental sample A, B, C and D, respectively. The contribution of TMAO and TMA to the extractive nitrogen was very low as they are occupying 0.7~1.2% in the four experimental samples.

Integrated Supply Chain Management is a important subject for all enterprise activities as include logistics/sales, transfer and storage, manufacturing, purchasing of materials. A recent customer wants to receive high level service of all parts as Qualify, Delivery, Cost and Product. Therefore, Enterprise effort to supply for customers needs use some techniques like Data Mining, POS, MIS. Inventory and Logistics cost is the highest expense of all cost from first supplier to final customer on supply routine. So, SCM's basic purpose is reduce to that cost. So that this paper explain necessary, background, concept of SCM, analyze several using methodology and function of main SCM solution, after propose to ATP model include arithmetic procedure, functions, input data for determines available due date.

최근 기업활동의 자재구매, 제조, 보관 및 운송, 유통/판매까지 포함한 공급체인 전체에 대해 통합관리가 중요한 비스를 받고 싶어한다. 이에 따라 오늘날의 대부분의 기업들은 고도의 기술을 요하는 정보시스템의 도입과, POS, Data Mining 등과 같은 기술들을 이용하고 고객의 요구 사항들을 적시, 적소에서 파악하여 고객이 요구하는 상품과 서비스를 공급하기 위하여 최선과을 정리하고 주요 SCM solution에서 제공하는 기능 및 사용된 방법론을 분석하여, 이를 바탕으로 주문 접수 시 주문물량의 가능납기를 산정하기 위한 입력데이터, 고려요소, 연산절차 등을 포함한 ATI(Available to Promise) 모델을 제제로 부각되고 있다. 현대의 고객들은 제품이나 납기, 품질과 같은 부문에서 높은 서의 노력을 다하고 있다. 최초의 공급자로부터 소비자까지 상품이 전달되는 과정 중에서 재고와 물류비용은 모든 비용의 대부분을 차지하고 있다. 이에 대한 비용을 줄이려고 하는 것이 공급체인관리(SCM)의 기본적인 목적이라 하겠다. 이에 본 논문에서는 공급체인관리(SCM)에 대한 개념과 배경, 필요성 등시한다.

In the supply chain, The ATP function doesn't only give customers to confirmation of delivery. It can be used by the core function with ATP rule that can reconcile supplies and demands on the supply chain. Therefore We can acquire the conformation about accuracy on the due date of supplier by using the ATP function of management about real and concurrent access on the supply chain, also can decide the affect about product availability due to forecasting or customer's orders through the ATP. This study analyze the data concerned with ATP and define the necessity on a SCM solution. Under the these environments, after defining the ATP rule that can improve the customer value and data flow related the CRM, we propose the advanced ATP model that proposes the method and classification system that can flexibly aggregate the ATP data with ATP rule on the supply chain.

공급망상에서 ATP는 단순히 고객에게 납기를 확약해 주는 고객 서비스 기능이 아니다. 이것은 공급 망상에서 ATP Rule을 기반으로 하여 수요와 공급 일치에 도달하기 위한 핵심 기능이다 따라서 SCM Solution을 이용하여 공급 체인 전반에 걸쳐 제품 가용성에 대한 즉시 및 동시 엑세스를 관리하여 기업의 납기 일의 정확도에 대한 최고의 확신을 가져올 수 있으며, SCM Solution을 통해 주문이나 예측 수주로 인하여 새로운 수요가 제품 가용성에 미칠 영향을 결정할 수 있다. 본 논문에서는 공급체인 전체의 통합관리 솔루션을 통하여 SCM에 대한 필요성을 정리하고 ATP관련 데이터를 분석을 수행한다. 이 것을 바탕으로 고객관계 관리( Customer Relationship Management)와 연계하여 세부데이터의 흐름 및 고객 가치를 향상 할 수 있는 ATP Rule을 정의한 후, 본 논문에서는 공급망상에 ATP Rule을 적용하여 ATP관련 데이터를 유연성 있게 취합할 수 있는 방안과 분류체계를 제시한 다단계 ATP모형을 제시 한다.

This study was conducted to investigate the application of ATP bioluminescence to measure the degree of microbial contamination from raw meat, meat processing and milk processing lines. Samples collected from slaughter house, meat and milk processing plants were tested for estimation of bacterial number by using ATP bioluminescence and conventional method. The former result was transffered to R-mATP value(log RLU/㎖), and the latter transffered to CFU(log/㎖). Correlation coefficient(r) between aerobic counts(CFU, log/㎖) and R-mATP(log RLU/㎖) value was 0.93(n=408). R-mATP of aerobic counts from beef, pork, chicken was 0.93(n=220), and that was 0.93(n=187) between meat processing and dairy processing plants. In addition, Correlation coefficient(r) between aerobic counts and R-mATP was 0.87(n=252) under 1 × 10^5/㎖ of bacterial count and 0.74(n=152) over l0^5 respectively.

에너지 요구 반응인 NADP 전환 반응계에 acetate kinase에 의한 ATP 생성계를 도입하여 NAD의 NADP로의 효율적인 전환을 시도하였다. 전환 균체는 세포막 투과성 향상을 위하여 toluene 처리 후 동결 건조한 B. ammoniagenes Cells를 사용하였으며, 전환 균체의 고정 화 담체로는 3% k-carrageenan을 사용하였다. 한편 ATP 생성계 효소인 acetate kinase는 E. coli로부터 추출 정제하여 사용하였다. 전환 반응의 최적 온도 및 pH는 각각 37℃, pH 7.5였다. 또한 10mM acetyl phosphate, 5mM ADF, 200mM inorganic phosphate, 10mM MgCl_2, 고정화 균체 250㎎/㎖ , acetate kinase 49.5mUnit/㎖에서 가장 높은 전환율을 나타내었다. 상기 최적 조건하에서 12시간 전환 반응시 5mM (340㎎/㎖)NAD가 3.6mM NADP로 전환되어 약 72%의 전환율을 나타내었다.

With the rapid growth of fresh-cut produce market, the South Korean fresh-cut industry is facing the challenge of ensuring food safety. As the estimation of the microbial numbers in fresh-cut produce processing lines (tools, and equipment) using the conventional microbiological techniques takes days, so there is a need for faster and easier monitoring methods. This study was conducted to investigate the use of ATP bioluminescence assay to measure the degree of microbial contamination from three actual fresh-cut processing lines. The samples collected from frech-cut vegetables, and fresh-cut fruits processing plants were tested for the estimation of the bacterial number, using the ATP bioluminescence and microbiological methods. The result of former was transferred to log RLU/100 cm2, and that of the latter was transferred to log CFU/100 cm2. A positive linear correlation between the ATP bioluminescence assay value and aerobic-plate count was found for fresh-cut processing lines, with a correlation coefficient of 0.8772 (n=50). The results of this study indicate that ATP bioluminescence assay can be used to monitor microbial contamination in fresh-cut produce processing plants, and can help improve the hygiene therein.

A cDNA clone encoding a MDR-like ABC transporter protein was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (named as Brmdr 1; GenBank accession no.: DQ296184 ) had a total length of 4222 bp with an open reading frame of 3900 bp, and encoded a predicted polypeptide of 1300 amino acids with a molecular weight of 143.1 kDa. The BrMDR1 protein shared 71.0, 62.5, 60.0 and 58.2% identity with other MDR proteins isolated from Arabidopsis thaliana (AAN28720), Coptis japonica (CjMDR), Gossypium hirsutum (GhMDR) and Triticum aestivum (TaMDR) at amino acid level, respectively. Southern blot analysis showed that Brmdr1 was a low-copy gene. Expression pattern analysis revealed that Brmdr1 constitutively expressed in the root, stem petals and stamens, but with lower expression in leaves and open flowers. The domains analysis showed that BrMDR1 protein possessed two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs) arranging in "TMD1-NBD1-TMD2-NBD2" direction, which is consistent with other MDR transporters. Within NBDs three characteristic motifs common to all ABC transporters, "Walker A", "Walker B" and C motif, were found. These results indicate that BrMDR1 is a MDR-like ABC transporter protein that may be involved in the transport and accumulation of secondary metabolites.

A number of field experiments were conducted to assess the role of combined application of nitrogen and sulfur to increase the seed and oil yield of nonnodulating soybean (Glycine max (L) Merr.) cv. PK-416 (V1) and cv. PK-1024 (V2) . Six combinations of N and S in three replicates each were used for this purpose i.e. 0~;S+23.5kg~;N~;ha-1(T1);~;0~;S+23.5+20kg~;N ~;ha-1(T2);~;40~;S+23.5kg~;N~;ha-1(T3);~;40~;S+23.5+20kg~;N~;ha-1(T4);~;20+20~;S+23.5kg~;N~;ha-1(T5);~;20+20~;S+23.5+20kg~;N~;hal(T6) . Nitrate reductase (NR) and ATP-sulphurylase activities in the leaves were measured at various growth stages as the two enzymes catalyze the rate limiting steps of the assimilatory pathways of nitrate and sulphate, respectively. The activities of these enzymes were strongly correlated with seed yield. The higher seed, oil and protein yields were achieved with the treatment T6 in both the cultivars due to optimization of NR activity and ATP-sulphurylase activity, as these parameters were influenced by N and S assimilation. Any variation from this combination was observed to decrease the activity of these enzymes resulting in reductions in the seed, oil and protein yield of soybean.