본 연구는 전라남도와 경상남도의 4개 시.군에서 개설 후 1∼15년 경과한 임도에서 경과년수별로 절토 사면에 조사구를 설치하고. 식생조사와 환경인자를 조사하였다. 경과년수에 따라 침입하는 종 수와 피복도는 점차 증가하였으며, 초본류의 출현비율은 감소하였고, 목본류의 출현비율은 증가하였다. Ordination 분석을 실시한 결과 종의 분포에 영향을 미치는 요인은 시공 후 경과년수, 절토경사, 해발고, 절토사면 방향 등으로 나타났다. 임도사면의 녹화를 위해서는 시공 초기에 파종하던 종 이외에 쑥. 뱀딸기, 참억새, 칡, 산초나무, 붉나무, 병꽃나무 등이 적합할 것으로 판단된다. 임도사면에 출현한 관속식물은 77과 190속 233종 38변종으로 총 271종이며. 그 중 귀화식물은 18종이 출현하였다. 출현종의 빈도 우선순위를 보면. 산딸기, 쑥, 산초나무, 칡, 소나무. 조록싸리, 큰까치 수영, 싸리. 이고들빼기. 참억새, 개망초 등의 순이다.

This study investigated the effect of vegetation mat on plant growth and salt reduction in the soil treated with high concentration deicing salt. In order to measure soil chemical characteristics and plant growth, three native groundcover plants (Dendranthema zawadskii var. latilobum, Dendranthema boreale, and Kalimeris yomena) were grown in each of the three plastic containers (50.0 cm width × 35.0 cm length × 8.5 cm deep) with a high concentration treatment of calcium chloride deicing salt. There were two treatments: control, and BVM that combines B (blanket) and VM (vegetation mat). 1,600 g of soil was placed on the top of the drainage layer with 290 g of perlite, 100 seeds each of the three native plants with three repetitions were sowed, and 10 g/L of calcium chloride deicing salt was added in the treatment. As a result of the chemical properties of soil, soil in control treatment was acidic and soil electrical conductivity in BVM was the lowest. Also, exchangeable cations (K+, Ca2+, Na+, and Mg2+) in soil and all the three plants were significantly decreased in the BVM treatment. Meanwhile, the germination rate of Dendranthema zawadskii var. latilobum was the highest under high concentration deicing salt in compared to the two plants. Overall, three native groundcover plant growth was higher in the BVM than control treatment significantly. These results suggest that the treatment of blanket vegetation mat has a positive effect on soil and plant growth in soil damaged by deicing salt.

The Wnt/β-catenin signaling pathway is mandatory in adipogenesis. In this study, we investigated the applicability of functional materials for the treatment of obesity by examining Wnt/β-catenin pathway reporter activity related to adipocyte differentiation inhibiting with korean native plant extracts. The luciferase activity of HEK 293-TOP cells increased the reporter activity approximately 152% and 130% by treatment with Sanguisorba officinalis and Thuja orientalis, respectively. Ricinus communis were represented about 90% higher activity, two samples(Rosa rugosa and Sophorae Flos) showed 80% higher activity than the control. Three samples of plant extracts (Zanthoxylum piperitum, Pueraria thunbergiana, Solanum nigrum) were about 70% higher activity compared with the non-treated control. Cytotoxicity of plant extracts was not detected in the rat neural stem cells. These results suggest that the selected eight plant extracts are safe compounds. Our findings indicate that Wnt/β-catenin pathway reporter activity could be used for high throughput screening system. In addition, the plant extracts selected as candidates for adipocyte differentiation inhibiting may be potential therapeutic agents for obesity, it will be exploring the possibility of developing an anti-obesity materials through further experiments with selected plant extracts.

Investigation of the plant growth affected by plug cell volume/pot size and plant age in herbaceous native plants belonging to 11 family and 24 species showed that growth of aerial part was different according to both plant age and plug cell volume/pot size. Among them, especially Iris dichotoma, Iris ensata var. spontanea, Iris pseudoacorus Lysimachia vulgaris var. davurica, Campanula glomerata var. dahurica and Solidago virgaurea subsp. asiatica showed more prominent difference depending on plug cell volume compared to other species. Regarding growth of subterranean part, as decrease plug cell volume/pot size root quality index became high while root weight became low, indicating that growth restriction was happened by exceed increase of root density due to limited root zone area. As increase cell volume/pot size, root development became active in bigger than 150 ㎖ of cell volume. Plant quality got better as increase cell volume/pot size, and 60 days old plants (DOP) revealed higher quality than 90 DOP revealed, indicating that marketing is possible from 60 DOP. Considering both plant age and cell volume (pot size), plant quality was better in smaller cell volume when cultivate plants in short time, while better in bigger cell volume when cultivate plants long time. Accordingly, when extend cultivation periods in small volume of plug cell, plant quality became bad. Therefore, this study showed that in order to produce high quality of plants, plants should be grown in proper plug cell volume depending on cultivation periods. Among plants tested Hosta longipes, Aquilegia buergeriana var. oxysepala, Aquilegia japonica, Veronica linariifolia, Hemerocallis fulva, Iris ensata var. spontanea, Iris pseudoacorus, Allium senescens, Hypericum ascyron, Caltha palustris var. palustris, Gentiana scabra, Campanula glomerata var. dahurica, Dendranthema boreale, Dendranthema zawadskii var. latilobum, Aster spathulifolius, Aster maackii, Aster koraiensis and Solidago virgaurea subsp. asiatica were determined to be able to produce standard size nursery plants using plug tray, and proper plug cell volume was bigger than 150 ㎖ (32 cell tray).

For developing natural preservatives and insecticide contained in volatile organic compounds of Elsholtzia ciliata and Agastache rugosa, the composition of two plants were analyzed and tested on microorganism survival and insectcide effect from TD-GC-MSD analysis. Main components of Elsholtzia ciliata were elsholtzia ketone, dehydroelsholtzia ketone (82%) and caryophyllene (10%). Main components of Agastache rugosa were estragole (88.2%), dl-limonene (4.5%), and trans-caryophyllene (2%). Inhibition activity of 70% methyl-alcohol extract of A. rugosa against growth of Enterobacter cloacae and Salmonella typhimurium was signifcant effect of observed clear zone and that of E. ciliata was against Pseudomonas aeruginosa, respectively. The mosquito larvae was totally died treated by 0.5g powder of A. rugosa for 3 hours, while 80% of the mosquito larvae was died in E. ciliata powder processing. Mortality of mosquito is the first 5 minutes after the smoked incense treatment showed 65% is E. ciliata and 80% in A. rugos and then finally all mosquito died after 20 minutes.

This study was conducted to select native ground cover plants for sod culture in an organic apple orchard by estimating the effect of three native ground cover plants, Glechoma hederacea, Thymus magnus, and Ixeris stolonifera, on the soil coverage, time-periodic weed occurrence, fruit characteristics, and soil chemical properties. The plant height of G. hederace, T. magnus and I. stolonifera were 15.0 ㎝, 13.4 ㎝ and 7.2 ㎝, respectively. The dry weight of G. hederace, T. magnus and I. stolonifera were 463 ㎏/10a, 247 ㎏/10a, and 255 ㎏/10a, respectively. The plant height and dry weight of G. hederacea were higher than in the other species. T. magnus and I. stolonifera having relatively lower soil cover rate during their life cycle produced a lot of weeds in the orchard as compared with the control. In contrast, G. hederacea showed 100 percentage of ground cover in the first year, and maintained high percentage of ground cover in the growing season of ‘Tsugaru’ apple for another 2 years. When the soil was covered with G. hederacea in the orchard for 3 years, the amount of weed was only 114 ㎏/10a and number of weeding was also reduced about 33% compared with control as well as the other species. There were no differences in the tree growth and fruit characteristics between the native ground cover plants and the control; however, positive effects of native ground cover plants on soil chemical properties were found. In G. hederacea, available P2O4content in soils remarkably increased and was a significant difference among native species. In addition, cation (Ca, Mg and K) content in soils increased by 39% in Ca, 6% in Mg, and 11% in K at G. hederacea compared with control. These results suggest that G. hederacea could be advantageous in terms of reducing the amount of herbicide applied and the labor required for weed control, and controlling soil chemical properties; therefore, it is a good candidate for sod culture in an organic apple orchard.

Lonicera caerulea var. edulis is a rare species found in some alpine region of Korea. Genetic variation in L. caerulea var. edulis has been investigated by examining 161 individuals from six natural populations: Mt. Seorak 1, Mt. Seorak 2, Mt. Jeombong, Mt. Bangtae, Mt. Gyebang, Mt. Halla. The mean genetic diversity for all the six populations was 0.25 (S.I.). The highest genetic diversity was found in Mt. Seorak (S.I.=0.3158) and the lowest was in Mt. Gyebang (S.I.=0.1047). Comparatively low level of genetic diversity was observed (Ae=1.25, P= 64.6%, S.I.=0.25), which is a typical pattern for rare tree species. AMOVA showed exceptionally large proportion of genetic variations both for among populations (34.69%) and within populations (65.31%). Excluding Mt. Gyebang, the genetic variation among and within population was 18.71% and 81.29% respectively. The UPGMA dendrogram based on genetic distance is not suitable for geographic relationship. Genetic distance of Mt. Gyebang was most distant from the other populations. Excluding Mt. Gyebang, the genetic identities among the five populations were 0.95 to 0.97, which is very high similarity level of genetic identity. This low level of genetic variations and the lack of site in nature indicates that L. caerulea var. edulis demanded a serious conservation.

본 논문은 정원문화를 대중화하기 위한 기초 연구로서 일반인이 식물을 쉽게 구입할 수 있는 온라인에서 자생초본식물의 판매현황을 파악하고자 하였다. 연구결과 2015년 6월 현재 인터넷에서 자생초본식물을 판매하는 업체는 130개소였으며, 그 중 자체 블로그나 홈페이지를 구축한 업체가 50개소, 통합사이트 2개소에 등록한 업체가 80개소였다. 일반인이 온라인에서 구입가능한 자생초본식물은 75과 248속 468종이었으며, 희귀식물 중 멸종위기종(CR), 위기종(EN), 취약종(VU) 31과 66속 87종을 판매하고 있었으며, 귀화식물은 10과 13속 14종 판매하고 있었다. 온라인에서의 식물 유통은 실제 식물을 보지 않고 판매업자가 구매자에게 주는 정보가 바탕이 되어 판매되므로 제공되는 정보의 범위와 정확성을 파악하였다. 조사결과 야생화의 범주에서 재배식물, 외래식물을 판매하는 업체가 74%였고, 초본식물을 야생화로 인지한 경우가 60%였다. 또한 야생화의 세부 분류 카테고리가 위계 없이 나열되어 소비자에게 혼란과 불편함을 줄 것이라 예상된다. 식물공급업자의 50% 이상이 식물의 시각적인 특성과 이용적인 특성과 같은 식물의 문헌적인 정보를 제공하고 있으나 수요자의 입장에서는 식재시 고려사항과 식재 후 관리방법 등에 대한 실질적인 정보 제공이 필요하다. 또한 구매할 식물에 대한 정보에서 식물명에 오류를 보인 업체가 74%나 되어 정확한 정보제공이 필요하다. 현황 조사 결과 온라인에서 자생식물의 유통을 활발하게 하려면 첫째, 다품종으로 소량 생산하는 영세한 업체들을 위해 자생식물을 전문으로 하는 포털사이트 구축이 필요하며 둘째, 무분별하게 판매되는 희귀식물에 대한 보존과 유통을 위한 관계기관의 법제정 또는 보완이 필요하며, 셋째 자생식물에 대한 정확한 정의와 명칭, 세부분류기준을 체계적으로 두어 대중들이 보다 쉽고 유용한 정보를 얻을 수 있도록 해야 한다.