The present management of the domestic and international advanced enterprises is transferring the systems and the regulations to the prevention system management. Expecially, KOSHA (Korea Occupational Safety & Health Agency) in Korea has supported to set up the OSHMS (Occupational Safety & Health Management System) by KOSHA 18001 since 1997. But small & medium enterprises (SMS) has many difficulties to set up the OSHMS system because of limited natural and human resources. In this study, the rating system as a judgment method of an OSHMS based on the certification standard of the OSHMS was suggested for SMS to adopt voluntarily the OSHMS, certify KOSHA 18001, and improve the safety and health level.

The introduction and application of IT(Information Technology) to a company has become to be viewed as a key factor for the business competitiveness and improved productivity. This study presents an approach to evaluation of a company's informatization le

들잔디의 최적 조직배양 조건을 확립하기 위하여 들잔디 성숙종자로부터 재분화능이 높은 배발생 캘러스 유도효율에 미치는 몇 가지 요인에 관하여 조사하였다. 성죽종자의 소독은 30% (v/v) NaOCl 농도로 60분간 처리하였을 때 가장 낮은 오염율과 가장 높은 캘러스 유도 효율을 보였다. 옥신류의 혼용처리에 따른 배발생 캘러스 유도효율은 2,4-D와 diacamba를 각각 3 mg/L로 혼용처리 하였을 때 가장 높은 배발생 캘러스 유도효율을 나타내었다.

We have estimated the fractal dimension of the molecular clouds associated with the H ΙΙ region Sh 156 in the Outer Galaxy. We selected the 12CO cube data from the FCRAO CO Survey of the Outer Galaxy. Using a developed code within IRAF, we identified slice-clouds (2-dimensional clouds in velocity-channel maps) with two threshold temperatures to estimate the fractal dimension. With the threshold temperatures of 1.8 K, and 3 K, we identified 317 slice-clouds and 217 slice-clouds, respectively. There seems to be a turn-over location in fractional dimension slope around NP (area; number of pixel) = 40. The fractal dimensions was estimated to be D = 1.5 ∼ 1.53 for NP ≥ 40, where P ∝ AD/2 (P is perimeter and A is area), which is slightly larger than other results. The sampling rate (spatial resolution) of observed data must be an important parameter when estimating fractal dimension. Fractal dimension is apparently invariant when varying the threshold temperatures applied to slice-clouds identification.

This study was designed to investigate the effect of essential amino acids (EAA) and/or non-essential amino acids (NEAA) on the development of parthenogenetic and somatic cell nuclear transfer (SCNT) porcine embryos in vitro. To evaluate the timing of amino acids supplementation, activated oocytes were cultured in NCSU23-PVA with EAA, NEAA or NEAA+EAA (AAs) during specific periods as below: EAA, NEAA or AAs were supplemented during Day 0 to 6 (whole culture period: ALL), Day 2 to Day 6 (post-maternal embryonic transition period: POST-MET), Day 5 to Day 6 (post-compaction period: POST-CMP), Day 0 to Day 2 (pre-maternal embryonic transition period: PRE-MET), or Day 0 to Day 4 (post-compaction period: PRE-CMP). Supplementation of NEAA decreased cleavage rates in PRE-MET and PRE-CMP and also decreased blastocyst rates in POST-CMP. On the other hand, EAA significantly enhanced blastocyst formation rate in POST-MET and no detrimental effect on embryonic development in other groups. Interestingly, NEAA and EAA had synergistic effect when they were supplemented to the medium during whole culture period. Supplementation of AAs also enhanced SCNT porcine embryo development whereas BSA-free medium without AAs could not supported blastocyst formation of SCNT embryos. In conclusion, existence of EAA and NEAA in defined culture medium variously influences the development of parthenogenetic and SCNT porcine embryos, and their positive effect are only occurred when both EAA and NEAA are supplemented to the medium during whole culture period. Additionally, AAs supplementation enhances the blastocyst formation of SCNT porcine embryos when they are cultured in the defined condition.

The objective of this study was to examine the effect of caffeine and sodium bicarbonate in a fertilization medium on the fertilizability of boar spermatozoa that were frozen in straws. Boar spermatozoa were extended with Beltsville F5 extender and frozen in 0.25‐ml straws. In vitro matured porcine oocytes were fertilized in vitro (IVF) with frozen‐thawed boar spermatozoa for 6 h in a modified tris‐buffered medium (mTBM) or in its modified medium by substituting the tris with 25 mM sodium bicarbonate (modified bicarbonate‐buffered medium; mBBM). Some of inseminated oocytes were fixed and stained for examination of sperm penetration. IVF embryos were cultured in a North Carolina State University‐23 medium for embryo development. The percentage of live sperm was 47±4% and morphological abnormality of acrosome was found in 14±3% of spermatozoa. Optimal sperm concentration for IVF was 0.75～1.0×106 sperms/ml when mTBM containing 5 mM caffeine was used as the fertilization medium. Sperm penetration was significantly (p<0.05) stimulated by increasing caffeine concentration in the IVF medium. In addition, mBBM significantly (p<0.05) increased sperm penetration (92%) compared to mTBM (65%). More (p<0.05) blastocysts (22% vs. 32%) developed from the oocytes that were fertilized in mBBM containing 1 mM caffeine than from those fertilized in mTBM with 5 mM caffeine. Our results indicate that boar spermatozoa can be frozen successfully in straws with holding their normal fertilizability and that caffeine and sodium bicarbonate stimulates sperm penetration in vitro.

In medium-sized enterprises that comprise of multiple business branches and companies, various types of information systems are constructed and operated. One of the difficult problems these enterprises face is that integrated information cannot be deliver

The present management of the domestic and international advanced enterprises is transferring the systems and the regulations to the prevention system management. Expecially, KOSHA (Korea Occupational Safety & Health Agency) in Korea has supported to set up the OSHMS (Occupational Safety & Health Management System) by KOSHA 18001 since 1997. But small & medium enterprises (SMS) have many difficulties to set up the OSHMS because of limited natural and human resources. Therefore, this study is developed and proposed the certification standard of an OSHMS for SMS based on the case study on advanced countries, actual conditions of SMS in Korea, and opinion of specialists.

This study was conducted to investigate the development and gene expression in miniature pig nuclear transfer (mNT) embryos produced under different osmolarity culture conditions. Control group of mNT embryos was cultured in PZM-3 for 6 days. Treatment group of mNT embryos was cultured in modified PZM-3 with NaCl (mPZM-3, 320 mOsmol) for 2 days, and then cultured in PZM-3 (270 mOsmol) for 4 days. Blastocyst formation rate of the treatment group was significantly higher than the control and the apoptosis rate was significantly lower in treatment group. Bax- and caspase-3 mRNA expression were significantly higher in the control than the treatment group. Also, the majority of imprinting genes were expressed aberrantly in in vitro produced mNT blastocysts compared to in vivo derived blastocyst H19 and Xist mRNA expression were significantly lower in the control than the treatment group or in vivo. IGF2 mRNA expression was significantly higher in the control than the treatment group or in vivo. IGF2r mRNA expression was significantly lower in the control. Methylation profiles of individual DNA strands in H19 upstream T-DMR sequences showed a similar methylation status between treatment group and in vivo. These results indicate that the modification of osmolarity in culture medium at early culture stage could provide more beneficial culture environments for mNT embryos.

본 시험은 이탈리안 라이그라스 내한 중생 신품종을 육성하기 위하여 1999년부터 2006년까지 축산과학원 조사료자원과에서 수행되었다. 이탈리안 라이그라스 신품종 '코윈마스터'는 2배체 품종으로서 엽의 색은 담녹이며, 월동전 초형은 중간형, 봄의 초형은 반직립형이다. 코윈마스터는 5월 13일경에 출수하는 중생 품종이었다. 특히, 신품종 코윈마스터는 Florida 80 품종과 지엽의 폭은 비슷하나 지엽의 길이가 3 cm 정도 길며, 초장은 89 cm로 Fl

Advanced countries worldwide lead government leading management innovation by suggesting unique managing system which can be survived in global economy war including JQA of Japan and EFQM of Europe based basically on MBNQA of U.S.A. and by rewarding to suitable companies. Mckinsey, global management and consulting company points out that Korea has no management because Korean productivity level which dependence to small and medium sized companies is high by the limitation of elemental invests leading type growth strategy including labor and capital is only 1/2 of U.S.A. and 2/3 of Japan. In particular, the competitive power of Korean small and medium sized companies goes into a recession by productivity lowering according to the chinese follow-up, laboring time shortening, variety and aging, and fundamental management innovation activities for reinforcing survival and competitive power are needed. Therefore, in this study, we try to construct the model of productivity managing system of innovation type small and medium sized companies which make excellent results.

RFID system the key technology of ubiquitous era, has been emphasized in logistics. Logistic enterprises are required to apply RFID systems considering their investment costs, actual effectiveness, operational satisfaction and so on. For such a purpose, many applicable methods of RFID system have been developed A method that RFID tags are directly attached to products or pallets is utilized in most cases. However, this method requires expensive investment cost, frequent replacement of damaged tags for the small and medium (SM) third party logistics. In this paper, we analyze statistically the current status and requirements of SM third party logistics. Based on their requirements, we propose the realtime warehousing-delivery management by the method to attach a RFID tag to each warehouse rack, together with the economy analysis index in order to evaluate economical efficiency. Consequently, this method is expected to reduce Investment cost and to improve operational effectiveness and satisfaction.

Product liability(PL) is the producer's compensation to customers for damage incurred by product defects. This paper studies on the organizational culture, promotion process, system construction, system level, product safety review, product safety assurance, system operation, and its effectiveness and necessity of recognition for the PL system of small and medium-sized firms in Gangwon-Do province. The results show that the firms have product safety regulations, but the number of firms which its employees understand the regulations is less than the number of firms which do not understand. Also the number of firms which is preparing PL prevention and insurance, and counterplan for accidents seems to be not in large numbers.

Measurement is used for evaluation of product or process exactly. If it couldn't measured correctly, Quality-cost must be raised and it would be hard to improve product quality. So, this study suggests improvement guide line for the multilateral problems of measuring instrument operation based on the investigation of 157 small and medium-sized enterprises in February, 2008. To use inspection gig correctly, man who treat it must be accustomed with the structure, the performance, the method. The inspection gig is selected properly for the measurement goal. If not, results couldn't be correctly or wasted time, efforts, and costs. When selecting a inspection gig, the locating, the clamping, and the efficiency must be considered.

The need for total enterprise management that covers from accepting orders, design, cost, procurement, manufacture, and delivery has been recently raised in order for efficient project management of small and medium size plant construction enterprises. This trend is reflected in the introduction of EPM(Enterprise Project Management) solutions by some shipbuilding enterprises and large construction corporations. In step with the trend, small and medium size plant construction enterprises are enabled to provide knowledge base in terms of reducing cost and production period and managing portfolio for accepting orders and attaining contract by inducing customized EPM This study presents a design of customized EPM based on a case study of a company. It exhibits a collaborative/collaboration resource-supply model which can be universally applied to small and medium size job-order production enterprises. The research discusses that under the assumption that information is shared internally between departments on planning, supply, manufacture, and quality control in order to create collaborative environment and close participation is created externally between suppliers and orderers, productivity can be improved in cost reduction through planning efficient collaborative supply model.

국내 재배환경에 적합한 다수성인 거베라 소륜계통 의 우수 신품종 육성을 위하여 경남농업기술원 화훼육종연구소에서 1999년부터 교배조합 육성을 위하여 국 내의 재배농가와 종묘업체로부터 품종을 수집한 후, 2001년 3월에 핑크색 대륜계의 ‘Grandeur’를 모본, 오 렌지색 중·대륜계의 ‘Songsongee’를 부본으로 인공교 배를 실시하여 교배조합으로부터 436개의 종자를 획득 하였다. 교배 후 결실이 된 조합은 채종 즉시 파종 하였으며, 파종 1개월 후 발아된 378개의 유묘는 본 엽이 4∼5매 전개되었을 때 화훼육종연구소 유리온실 내의 선발 포장에 정식하였다. 그 후 교잡 1세대를 육 묘하여 화색이 선명하고 수량이 많은 우수 5개체 (01129-028, 105, 188, 325, 335)를 1차로 선발하였 다. 그 후 특성이 우수한 01129-335(경남 G-24)개체 를 생장점배양에 의해 개체증식 후 화훼육종연구소 유 리온실내의 특성검정 포장에 정식하여 2003년 6월부터 2004년 10월까지 3회에 걸쳐 생육 및 수량특성 검정 을 실시하였다. 특성검정을 실시한 결과 화색, 화형, 수확량 등 품질과 수량이 우수하다고 판단되어 2005 년 농작물 직무육성 신품종선정위원회의 심의를 거쳐 ‘Sweety’로 명명하였다. ‘Sweety’는 화색이 선명한 오 렌지색의 반겹꽃 절화용으로 수량이 많고 안정된 중· 소륜화로서 화색과 화심색이 잘 조화된 기호도가 높은 품종이다. 본당 수량은 연간 3회 검정 결과 평균 51.2개이며, 평균 절화수명은 10.4일이다.

플러그 육묘된 일일초와 살비아의 묘를 145mL 용적 의 사각형 플라스틱 포트에 peat(60%)와 perlite(40%) 로 구성된 인공토양을 채운 후 정식하였다. 완효성 비 료의 양이 배지의 EC와 식물의 생장에 미치는 영향을 구명하기 위하여 포트당 완효성 비료를(14-14-14 Osmocote, 14N-6.2P-11.6K) 0, 0.5, 1.0, 1.5, 2.0 또는 4.0g으로 각각 정량하여 정식 전 배지와 혼합하였다. 식물은 1일 1회 수돗물을 저면 관수하며 재배하였다. 배지의 EC는 식물의 종류와 관계없이 완효성 비료의 양이 증가할수록 높았다. 두 식물 모두에서 낮은 비료 농도에서는 식물체의 생육이 진전되어도 배지의 EC가 변하지 않았으나 2.0~4.0g 처리에서는 생육이 진전되 면서 배지의 EC가 낮아졌다. 일일초의 최대 엽면적, 초장과 건물중은 완효성 비료의 양이 2.0~4.0g 처리했 을 때 얻었으며 이때 배지의 EC는 생육 전기간 동안 1.0~1.7dS ·m−1 범위에 있었다. 살비아의 엽면적, 건물 중과 엽록소 함량은 완효성 비료의 양이 많을수록 증 가해서 4.0g 처리에서 최대를 보였으며 이때 배지의 EC는 생육 전기간 동안 1.0~4.0dS ·m−1 범위에 있었 다. 살비아의 초장은 2.0~4.0g 처리에서 최대를 보였다. 완효성 비료의 양이 증가 할수록 일일초의 식물체 내 N, P, K, Mg, S함량은 높았던 반면 Ca함량은 낮 았다. B와 Mn의 함량은 비료의 양이 적을수록 증가 하였다.