본 연구는 상추 기르기 과학 탐구 프로그램을 초등학교 재량활 동시간에 적용하여 저학년과 고학년의 기초탐구능력에 미치는 영 향을 알아보고자 수행되었다. 이를 위하여, 초등학교의 2, 5학년 각 1개의 학급씩 실험군과 대조군으로 나누었고, 실험군에는 재량활 동시간을 활용하여 상추 기르기 과학 탐구 프로그램이 제공되었다. 실험군의 상추 기르기 활동에 기초한 원예-과학 통합프로그램 시 행 후, 저학년과 고학년 모두 기초탐구능력 총점이 사전보다 각각 3.89, 1.76으로 증가하였고, 저학년이 고학년과 비교하면 2.13점 더 향상되었다. 저학년의 기초탐구능력 하위 요소별 사후 검사 결 과, 대조군은 관찰을 제외한 분류, 측정, 추리, 예상에서 유의차 없 이 향상되었으나, 실험군은 사후 검사결과 관찰, 분류, 측정, 추리, 예상 모든 탐구요소에서 유의하게 향상되었다. 고학년의 기초탐구 능력 하위 요소별 사후 검사 결과, 실험군은 분류와 추리 영역에서, 대조군은 분류 영역에서 유의한 증가가 나타났다.

Seed & Variety Service for Plant Variety Protection (PVP). We previously constructed DNA profile database for identifying 159 lettuce varieties using 60 simple sequence repeat (SSR) markers. In this study, we selected optimum markers from previously applied markers and new SSR markers for the standardization of DNA profile database of 65 commercial lettuce varieties containing 18 PVP varieties distributed in Korea. Twenty-eight SSR markers from 60 SSR markers were selected for characterization with 65 commercial lettuce varieties according to the reproducibility, polymorphism, band pattern of marker and easiness of scoring. Out of 156 SSR primers, we additionally selected 11 new SSR primer pairs showed polymorphisms between 8 varieties and repetitive reproducibility on capillary electrophoresis system. Totally 127 polymorphic amplified fragments were obtained using 39 SSR markers. Two to seven SSR alleles were detected for each locus with an average of 3.3 alleles per locus. Average polymorphism information content was 0.517, ranging from 0.281 to 0.771. Genetic distance of clusters ranged from 0.29 to 0.96 by unweighted pair-group method with arithmetical average based on Jaccard’s distance coefficients. A total of 65 commercial lettuce varieties were discriminated by 39 SSR marker genotypes. These SSR profile database developed will be continually characterized for the standardization of DNA profiles for lettuce commercial varieties

식중독 유발 바이러스인 HAV는 제 1군 감염병원으로 규정되면서 감염 시 원인식품을 빠르게 분석하게 되었으며 그로 인해 정확하면서 빠른 검출기술을 요구하게 되었다. 본 연구에서는 HAV에 오염된 상추에서 신속하게 바이러스를 검사하기 위해서 IMS를 통하여 신속하게 HAV를 순수 분리 및 농축하였고, 형광물질인 quantum dot을 활용하여 형광검출을 실시하였다. 또한 일반적으로 바이러스 농축에 사용되는 PEG 농축방법과 비교하였을 때 검출능은 유사한 결과를 얻었으나, 농축시간 면에서는 IMS를 통한 방법이 효과적이었다. 또한 IMS 방법으로 확보된 항원을 Quantum dot을 활용하여 10분 이내에 바이러스를 검출할 수 있었다. 본 연구에서 제시된 검출기반은 식품 유통 과정 중 다양한 식중독 바이러스로부터 소비자를 보호 할 수 있는 검사방법으로 활용될 수 있다고 기대된다.

The objective of this study was carried out to elucidate the effect of LEDs (light emitting diodes) irradiation in relation to early growth and inorganic elements in leaf lettuce (Lactuca sativa L. ‘Chung Chi Ma’). In morphological changes of leaves, shoot elongation and hypocotyl length showed poor growth in red light irradiation, while the red+blue light irradiation induced shorter plant height and much greater leaf numbers resulting in increased fresh weight. In change of the Hunter's color and SPAD values, lettuce seedlings grown under in red+blue and fluorescent light irradiation had a higher a* value, otherwise SPAD values were not changed in these light irradiations. Interestingly, relative chlorophyll contents showed 1.8 times increased redness in the treatment of red+blue light irradiation. Inorganic element (N, Ca, Mg, Mn, and Fe) and ascorbic acid contents were increased in lettuce plants grown under LEDs light irradiation compared to those of lettuce grown under the fluorescent light which showed higher P and Mn contents. In conclusion, it is considered that red+blue light irradiation which stimulates growth and higher nutrient uptake in leaf lettuce could be employed in containers equipped with LEDs.

이 연구는 폐쇄형 식물공장 시스템 내에서 LED 펄스광의 튜티비 및 광도(PPFD)가 상추의 생장 및 광합성률에 미치는 영향을 구명하기 위해 수행하였다. 파종 3주 후 ‘청치마’ 및 ‘청치마’ 실생묘를 bar-type LED(red:blue:white = 5:2:1) 하에서 4주간 생육시켰다. LED 펄스(on/off) 간격은 연속조사(continuous), 200/200μs, 133/266μs, 100/300μs의 4가지로 설정하였는데, 이를 듀티비(duty ratio, DR, 한 주기 대비 점등시간 비율)로 표시하면 각각 100%, 50%, 33%, 25%였다. 이때 광도(PPFD)는 DR 100, 75, 50% 처리에서는 두 수준[고광(high light, HL), 저광(low light, LL)]으로 처리되었고, 광주기는 16시간이었다. 식물공장 내부의 온도는 주야간 20±2°°C, 상대습도는 약 70±10%로 유지되었다. 양액은 온도 20±2°C, EC는 1.2mS·cm-1, pH는 5.8±0.2로 유지되었고, NFT 방식으로 공급되었다. 처리 4주 후 ‘청치마’ 상추의 생체중과 건물중은 DR 100% HL 하에서 가장 높았고, DR 75% HL 및 DR 50% HL과는 유의차가 없었다. 엽수, 엽면적, 잎 두께(비엽면적의 역수)는 DR 100%, 75%, 50%의 HL처리에서 유의하게 큰 값을 나타내었다. 광이용효율(LUE)은 PPFD에 관계없이 연속광보다 펄스광 처리에서 높게 나타났으며, 특히 DR 25% HL 하에서 가장 높았고, DR 75% HL과 DR 50% HL 처리도 DR 100% HL보다 높은 값을 보였다. ‘적치마’ 상추의 생체중과 건물중은 DR 100% HL 하에서 가장 컸고, DR 100% LL, DR 75% HL, DR 50% HL 하에서 두번째로 컸다. LUE는 ‘청치마’와 같이 연속광보다 펄스광 하에서 높았다. 두 품종의 광합성 속도는 적산 PPFD에 비례하였으며, DR의 효과는 적었다. 결론적으로 펄스광은 상추의 LUE를 높이므로 LED를 광원으로 사용하는 폐쇄형 식물공장의 에너지 절감에 기여할 수 있을 것이다.

‘고홍’은 ‘DL 1672’와 ‘청치마’를 교배하여 육성한 잎상추 로 누운타원형 잎을 가진 축면 상추이다. 2005년 교배하여 2010년까지 계통육종법에 선발과 고정을 하였다. 신품종 ‘고 홍’은 회색 종자이며 엽형은 누운타원형이다. 잎 상단부는 붉 고 광택이 좋으며 잎 기부는 녹색이다. 다수성 품종으로 기존 품종보다 주당 45매 정도의 잎을 수확할 수 있으며, 주당 무 게는 평균 331 g로 12% 수량성이 많은 품종이다. 전국 6개지역(대관령, 경기, 충북, 전북, 경남, 제주)에서 1년간 봄, 여 름, 가을 재배하였을 때 평균 2,737 kg/10 a을 나타내었다. 특히 수량성에서 봄작형은 36.7톤, 가을작형에서 16.7톤이 생 산되어, 대비품종에 비해 34%, 23% 증수되는 다수성 품종이 다. 숙기는 정식 후 25일 후부터 수확이 가능한 조･중생종이 며, 고온기 비가림하우스 재배시에도 적색이 발현되는 특성을 가지고 있다. 적색발현의 주 요인인 안토시안닌 함량은 7.5 mg/ 100 g으로 대조품종인 ‘뚝섬적축면’보다는 고온기 적색발현이 좋은 품종이다. 쓴맛 성분인 락투신(latucin+8-deoxylactucin +lactucopicrin)은 ‘뚝섬적축면’보다 적어 쓴맛이 적고 단맛이 나며 아삭아삭하여 맛이 좋다. 이것은 잎두께와 엽연이 ‘뚝섬 적축면’보다 조밀하고, 잎의 경도가 21.1 kg/cm 2로 ‘뚝섬적축 면’보다 강한 특성을 가지고 있기 때문이다. 따라서 신품종 ‘고홍’은 봄과 가을작형에 가장 적합한 품종으로 추천할 수 있다.

The effect of phytoncide solution treatment on the browning and quality of fresh-cut iceberg lettuce during storage was studied. The treatments were applied as four solutions adjusted at 10℃: distilled water (DW) as the control; edible ethanol (EE); 1% (v/v) phytoncide essential oil from pine needle diluted with distilled water (DP); and 1% (v/v) phytoncide essential oil diluted with edible ethanol (EP). Fresh-cut lettuce was dipped in each solution for 60 sec, was rinsed with distilled water, was packaged with an OPP film bag, and was then stored at 4℃ for 12 days. The EP group had a significantly high level of total soluble solids, titratable acidity, and carbon dioxide, and low total bacteria counts, pH, and oxygen. The sensory score of color in the EP group recorded a high value, but the EE and EP groups recorded low scores in aroma and taste during the storage period. Alcohol and phytoncide were vaporized by opening the package for two hours, and the score of the aroma and taste of EP showed no differences from those of the other groups. Based on the above results, it was determined that the phytoncide essential oil diluted in edible alcohol with 1% solution inhibited the browning of and microbial growth in fresh-cut lettuce, and will be a useful natural compound in maintaining the quality of fresh-cut produce.

Enzymatic action and microbial growth degrade the quality of fresh-cut lettuce. Browning, a bad smell, and softening during storage are the major forms of quality deterioration. Health-oriented consumers tend to avoid foods treated with chemicals to maintain their freshness. This study was conducted to evaluate the change in the quality of fresh-cut lettuce with combined low-temperature blanching (LB) and ultrasonication (US). The optimum condition was selected using the response surface methodology (RSM), through a regression analysis with the following independent variables; the ultrasonication time (X1), blanching temperature (X2), blanching time (X3), and dependent variable; ΔE value (y). It was found that the condition with the lowest ΔE value occurred with combined 90s US and 45℃ 90s LB (US+LB). The combined treatment group (US+LB) was stored at 10℃ for 9 days with the control group and each single-treatment group, with low-temperature blanching and ultrasonication. Overall, the US+LB group had a significantly high L* value, which indicates significantly low a*, b*, ΔE, browning index, PPO, and POD activity values, and a low total bacteria count (p < 0.05). The US+LB group also had the highest sensory score (except for aroma and texture; p > 0.05).

According to lifestyle changes, the consumers’ concern about food also shifts from calories and nutrition to health and convenience. Fresh-cut produce is one of the new turns in the consumption pattern of fruits and vegetables. The increasing demand for it requires processors to make them stable in quality and safe from microorganisms. The results of the evaluation of the production process and hygienic management of fresh-cut lettuce revealed that the facilities used, such as the drainage holes, floors, and door knobs, were severely contaminated with microbes, and that the work equipment, workbenches, landing nets, and centrifuges were highly contaminated. Accordingly, improved production processes and management systems are necessary, as is the implementation of a quality control system from the stage of raw-material purchase to the distribution stage.

The protective effect of nitric oxide (NO) on the antioxidant system under paraquat(PQ) stress was investigated in leaves of 8-week-old lettuce (Lactuca sativa L.) plants. PQ stress caused a decrease of leaf growth including leaf length, width and weight. Application of NO donor, sodium nitroprusside (SNP), significantly alleviated PQ stress induced growth suppression. SNP permitted the survival of more green leaf tissue preventing chlorophyll content reduction and of higher quantum yield for photosystem Ⅱ than in non-treated controls under PQ exposure, suggesting that NO has protective effect on chloroplast membrane in lettuce leaves. Flavonoids and anthocyanin were significantly accumulated in the leaves upon PQ exposure. However, the rapid increase of these compounds was alleviated in the SNP treated leaves. PQ treatment resulted in lipid peroxidation and induced accumulation of hydrogen peroxide (H2O2) in the leaves, while SNP prevented PQ induced increase in malondialdehyde (MDA) and H2O2. These results demonstrate that SNP serves as an antioxidant agent able to scavenge H2O2 to protect plant cells from oxidative damage. The activities of two antioxidant enzymes that scavenge reactive oxygen species, superoxide dismutase (SOD) and catalase (CAT) in lettuce leaves in the presence of NO donor under PQ stress were higher than those under PQ stress alone. Application of 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3- oxide (PTIO), a specific NO scavenger, to the lettuce leaves arrested SNP mediated protective effect on leaf growth, photosynthetic pigment and antioxidant systems. However, PTIO had little effect on lettuce leaves under PQ stress compared with that of PQ stress alone. The obtained data suggest that the damage caused by PQ stress is in part due to increased generation of active oxygen by maintaining increased antioxidant enzyme activities and SNP protects plants from oxidative stress. From these results it is suggested that NO might act as a signal in activating active oxygen scavenging system that protects plants from oxidative damage induced by PQ stress and thus confer PQ tolerance.

The effects of seawater on growth of fettuce(Lactuca sativa L.), leaf perilla(perilIa frutescens var. japonica Hara), red pepper(Capsicum annuum L.) and cucumber(Cucumis sativus L.) seedlings were investigated in the glass greenhouse. These effects were studied on seedlings, and diluted seawater (1%, 5%, 10%, 20%, 50%, 100% v/v) was sprayed enough on feaves. The tested four vegetable crops have well grown up to 10% diluted seawater, but the tested vegetable crops were damaged from increasing salt levels. Of these, lettuce was provided salt-tolerant vegetable crop and red pepper was considered salt-sensitive vegetable crop. The salt tolerance of vegetable crops is different between crops and complicated because of additional detrimental effects caused by accumulated ions or specific ion toxicities in their leaves. These results show that agricultural use of seawater may be benefit crop cultivation in organic farming system as well as in conventional farming system.

‘춘풍적축면’은 ‘포자비적축면’과 ‘먹치마’를 교배 육성한 잎상추로 누운타원형 잎을 가진 축면 상추이다. 2000년 교배하여 2007년까지 계통육종법에 선발과 고정을 하였다. 전국 6개 지역(대관령, 경기, 충북, 전북, 경남, 제주)에서 2년간 봄, 여름, 가을 재배하였을 때 평균 2,069 kg/10a을 나타내어 기존 품종보다 주당 53매 정도의 잎을 수확할 수 있으며, 주당 무게는 평균 372 g으로 6% 수량성이 많은 품종이다. ‘춘풍적축면’은

미홍은 "단홍적축면"과 "뚝섬적축면"을 교배하여 육성한 추대가 늦고, 엽색이 매우진한 적축면 잎상추 품종이다. 기존 같은 type의 품종보다 추대가 늦은 중만생 품종이며, 적색발현이 좋아, 안토시안닌 함량이 28.9 mg/100 g으로 "뚝섬적축면"보다 10배이상 많았으며 수확 후 4℃ 수식 이미지에서 3주간 이상 저장이 가능하였다. 재배적기는 연중재배가 가능하나 시험재배 결과 평난지에서는 봄, 가을에 고랭지에서는 여름재배가 가능하

고풍적축면은 "안동적축면"과 "뚝섬적축면"을 교배하여 육성한 적축면 잎상추 품종이다. 기존의 같은 type의 품종보다 추대가 늦은 중만생 품종이며, 적색발현이 좋아, 안토시안닌 함량이 29.4 mg/100 g으로 "뚝섬적축면"보다 10배이상 많았으며 수확후 4℃ 수식 이미지에서 3주간 이상 저장이 가능하였다. 재배 적기는 연중재배가 가능하나 시험재배결과 평난지에서는 봄, 가을에 고랭지에서는 봄, 여름, 가을에 노지 및 비가림하우스

마이크로버블을 이용한 세척시간과 세척방법에 따라 처리한 상추의 세척효과와 저장 중 품질 변화를 살펴본 결과는 다음과 같다. 세척시간 설정을 위한 실험을 통해 마이크로버블로 1, 3, 5분간 세척하여 미생물변화, 관능검사, 세척된 정도를 측정한 결과 5분간 처리한 시료가 가장 좋은 결과를 나타냈다. 세척방법에 따라 전처리한 상추의 미생물 변화는 세척처리 한 직후 무세척(CT)구는 4.30 log CFU/g, 손세척(HW)구는 4.10 log CFU

유통현장에서 다양한 포장 형태로 이용되는 상추의 포장방법별 선도 유지 효과를 구명하고자 하였다. 현장에서 이용되는 상추의 소포장 형태를 포장재 종류 및 천공 여부, 포장 방법 등에 따라 봉지 형태의 PP 필름 무공 밀봉 포장, PP 필름 롤 타입 포장, PP필름 유공 포장, PET 상자형 용기 포장, LDPE 필름 봉지 형태 포장과 같이 5가지로 분류하였다. 포장 형태에 따라 상추를 저온에 저장하면서 중량 변화, 호흡량, 외관의 품위, 색상 변화

The MYB transcription factors play important roles in the regulation of many secondary metabolites at the transcriptional level. We evaluated the possible roles of the Arabidopsis R2R3-MYB transcription factor genes in flavonoid biosynthesis as they are induced by UV-B irradiation but are largely unexplored in terms of their associated phenotypes. We found that one member of this gene family, AtMYB60, inhibits anthocyanin biosynthesis in the lettuce plant. Wild type lettuce normally accumulates anthocyanin, predominantly cyanidin and traces of delphinidin, and develops a red pigmentation. However, the accumulation of anthocyanin pigments in AtMYB60 overexpressing lettuce was inhibited. We further found a complete absence of DFR transcripts in AtMYB60 overexpressing lettuce, whereas other biosynthetic genes in the anthocyanin metabolism pathway were expressed. To provide genetic tools the regulation of seed color of rapeseed which has been target for fuel, AtMYB was overexpressed in rapeseed. Transgenic plants showed lighter seed color and improved tolerance to abiotic stress than the wild type plants. The elucidation of the roles of the AtMYB60 transcription factor will facilitate further studies and provide genetic tools to better understand the regulation in plants of the genes controlled by the MYB-type transcription factors.

A new red leaf lettuce (Lactuca sativa L.) cultivar, “Jangsu” which has late bolting and good red expression leaf color was developed from a cross of “Yeolpungcheokchima” (late bolting and high yield) and “Seoulyakchima” (dark red color). The selection and breeding of advanced lines were done by pedigree method during 2000-2004. The advanced lines were evaluated for yield and adaptability in Korea (Gangwon, Kyunggi, Chungbuk, Kyungnam, Jeonnam, Jeonbuk, and Jeju) from 2005 to 2006. The “Jangsu” has gray seed color and elliptic thick leaves. Compared to “Yeolpungcheokchima”, marketable yield of “Jangsu” was lower by 10% (at 469 g per plant), but with particularly improved good expression of red leaf color in high temperature cultivation in the field. The shelf-life of “Jangsu” was three weeks longer than “Yeolpungcheokchima” at 4℃. The amount of bitter sesquiterpene lactones (BSL) and sugar content of “Jangsu” were similar with “Yeolpungcheokchima” at 46 ㎍/g and 4.8 brix, respectively, but taste is better, more crispy, and sweeter than “Yeolpungcheokchima”.