목적 : 한국인을 대상으로 혈청 25(OH) 비타민 D 농도와 근시와의 연관성을 알아보고자 하였다. 방법 : 국민건강영양조사 2010년도 자료를 이용하였으며, 나이는 10세 이상부터 69세까지 총 5,488명을 대상으로 하였다. 근시의 정도는 등가구면 굴절력을 기준으로 -3.00<SE≤-0.50 D를 경도 근시, -6.00<SE≤-3.00 D를 중등도 근시, SE≤-6.00 D를 고도 근시로 정의하였다. 혈청 25(OH) 비타민 D 농도는 20 ng/mL 이하일 경우 부족군으로 20 ng/mL 초과일 경우 정상군으로 정의하였다. 피어슨이 상관분석과 공변량을 보정한 복합표본 로지스틱회귀분석을 시행하여 혈청 25(OH) 비타민 D 농도와 근시와의 연관성을 평가하였다. 결과 : 혈청 25(OH) 비타민 D 농도와 등가구면 굴절력은 양의 상관성을 보이는 것으로 나타났다(r=0.132, p<0.001). 근시의 위험 인자는 나이, 가구 소득, 교육수준, 햇볕 노출, 비만, 당뇨, 고혈압, 백내장 그리고 안질환 가족력으로 나타났다. 혈청 25(OH) 비타민 D 농도가 20 ng/mL 이하일 경우 위험 인자를 보정한 후에도 고도 근 시의 위험이 2.55배(95% 신뢰구간: 1.27~5.16) 증가하였다. 결론 : 한국인에서 고도 근시는 혈청 25(OH) 비타민 D 농도와 연관성이 있었다.

본 연구는 복합운동이 여성노인의 폐기능, 혈중 비타민 D, 칼슘 및 골대사호르몬에 미치는 영향을 구명하기 위해 만 65세 이상 여성노인을 대상으로 운동군(n=13), 대조군(n=17)으로 구분하여 주 3회, 회당 60분의 복합운동을 실시하였다. 유산소 운동 강도는 1-4주는 40-50%HRR(RPE 12-13), 5-8주는 50-60%HRR(RPE 13-14), 9-12주는 60-70%HRR(RPE 14-15)의 강도로 설정하였고 저항 운동 강도는 1-4주는 OMNI-RES(3-4), 5-8주는 OMNI-RES(5-6), 9-12주는 OMNI-RES(7-8)강도로 설정하였다. 그 결과 폐기능 중 FEV1은 그룹×시기 간 상호작용 효과가 나타났고 운동군의 FVC/FEV1이 유의하게 증가하였다. 비타민 D는 그룹×시기 간 상호작용 효과가 나타났고, 운동군과 대조군 모두 유의하게 증가하였다. 칼슘은 그룹×시기 간 상호작용 효과가 나타났으며 대조군이 유의하게 감소하였다. 골대사호르몬 중 칼시토닌과 오스테오칼신은 그룹×시기 간 상호작용 효과가 나타났고, 오스테오칼신은 대조군이 유의하게 감소하였다. 따라서 본 연구의 결과를 통해 12주간의 복합운동이 여성노인의 신체활동을 활발하게 하여 폐 기능을 개선하고 혈중 비타민 D의 결핍을 완화할 수 있다고 생각되지만 칼슘 및 골대사호르몬에서는 유의미한 결과를 나타내지 못하였다.

Vitamin D contents in agricultural products and foods were quantified by high performance liquid chromomatography (HPLC) with a UV/Vis detector, using external standard methods. The results were confirmed with liquid chromatography tandem mass spectrometry (LC-MS/MS). After homogenization, samples were hydrolyzed by direct alkali saponification. Thereafter, fat-soluble components were extracted with n-hexane containing 0.01% butylated hydroxytoluene (BHT). Vitamin D contents in cereals were found to be in the range of 1.882~4.856 μg/100 g. Juda's ear and oak mushroom contained high amounts of vitamin D, at 363.85 and 199.42 μg/100 g of edible portion, respectively.

The purpose of this study was to investigate the effect of calcium (Ca) and vitamin D (vit. D) levels on metabolism of various minerals such as Ca, P, Mg, Fe, Zn, Cu, and Cr. The comparison was made on the rats that were placed on diet containing powdered skim milk with different Ca and vit. D levels for 5 weeks. A total of 42 5-week-old Sprague- Dawley rats were divided into 7 groups as follows: Control group consisted of normal Ca and normal vit. D (0.5% Ca, 1,000 IU vit. D); Experimental groups were divided into low (0.25%) and high (1.0%) calcium levels; and vit. D group was divided into low (10 IU), normal (1,000 IU), and high (5,000 IU) subgroups. The weight gain and food efficiency ratios of the rats were not significantly different with increasing dietary Ca levels. The absorption rates of 7 minerals (Ca, P, Mg, Fe, Zn, Cu, and Cr) were significantly decreased with increasing dietary Ca levels. Also, fecal excretion of P significantly increased with increasing dietary vit. D levels (p<0.05), and urine excretion of Fe was significantly increased with increasing dietary vit. D levels (p<0.001). The result indicated that higher Ca intake affected on bioavailability of other minerals, due to interactions among minerals in the process of intestinal absorption. However, vitamin D intake had no effect on bioavailability of several minerals. Therefore, it could be suggested that adequate Ca intake is important for balance of the minerals.

We studi ed the difTerential elTect of vitamins A, C, U. and E on normal human 이 al keratinocyte(NHOK) , HPV-16 E6E7 immor talized human oral keratinocyte(1HOK) , Oncogene transfected HPV-16 immortalized ce1ls(OTOK) , and two ol'al sq ua mous cell line(HNSCC30‘ HNSCC31) according to carcinogenesis stage. The vitamin effect was evaluated by morphology. ce ll viabi lity. a nd orgnaotypic culture Vitamin A has a greater negative effect on growth for all NHOK IHOK HNSCC. es pec ially N-Ras t rans fected IHOK, Vitamin D & E revealed no significant cell activity on NHOK lHOK, ad OTOK Vitamin C was found increased cell viability to IHOK and OTOK 1n primary oral squmaous cell ca rcino ma (HN30 ). vitam in 0 and C showing increased cell growth , but vitamin E showing no effect 1n metastatic oral squamous cell ca rcinoma(HN31), vitamin C has prol iferative effect , but vitamin 0 & E has anti-proliferative effect Vitamin A t reated normal a nd ma lignant ce1ls by organotypic cu lt ure. showed reduction of epithelial layer and in vasion to connective tissue. , especia lly in 1HOK & oncogene-transfected 1HOK, 1n conclusion. three-dimensional culture sys tem may be useful as a model to acess the efficiency of agents such as a1l trans retinoic acid can preventing progression of these premaligant lesion to maligant oral carcinoma(ch emopreventive agent) .

This study was performed to investigate the induction of experimental atherosclerosis in rats and inhibitory effects of aloe vera on progression of atherosclerosis in rats. A dose range finding study of cholesterol and vitamin D₂ for the induction of atherosclerosis and studies on the subchronic effect of aloe vera and on the chronic effect of aloe vera were carried out. A total of 3-week old 125 male rats of Sprague-Dawley were divided into 25 groups and fed with the diet containing cholesterol (0.1, 0.2, 0.3, 0.5, 1.0 and 2.0%) and vitamin D₂ (500, 5000, 50000 and 500,000 IU/100 g) for 4 weeks. 35 male rats were divided into 7 groups and fed with the diet containing aloe vera with 1.0% of cholesterol and 50,000 IU/100 g of vitamin D₂ for 4 weeks. 200 male rata were divided into 5 groups and fed with cholesterol and vitamin D₂ for 6 and 12 months. Growth, clinical and pathological changes of rats in the three experiments were observed. The results were as follows: 1. In the dose-range finding study, feed intake, feed efficiency ratio and weight gain were significantly decreased and relative liver, heart, kidney and stomach weight to body weight were increased in all of the feed groups containing 500,000 IU/100 g of vitamin D₂. Serum biochemical values of total cholesterol, high-density lipiprotein cholesterol (HDL-cholesterol), triglyceride, calcium, inorganic phosphorous and chloride of male rats in treated groups. The aorta and coronary artery of rats in all of the diet group containing 500,000 IU/100 g of vitamin D₂ showed typical atheroaclerotic lesions. 2. Male rats fed with the diet containing aloe vera with 1.0% cholesterol and 50,000 IU/100 g of vitamin D₂ for 6 and 12 months did not show significant difference of diet intake and weight gain, and relative organ weight. The level of serum HDL-cholesterol and triglyceride recovered to the normal range by the aloe vera ingestation. 3. The aorta showed irregular appearence in the tunics intima with swelling, necrosis and calcification. The aorta of rat fed aloe vera diet showed no pathological lesions such as atherosclerosis of aorta. Aloe vera could have a helpful effect of vitamin D₂ and cholesterol induced atherosclerosis in rats. Long-term supplementation of aloe vera may slow down the process of experimental atherosclerosis in rats have effects on the development of atherosclerosis.

Ergocalciferol is known as having vitamin D activity. In this study, the effects of UV irradiation on the increase of egocalciferol content were investigated in 7 kinds of mushrooms, i.e, lily mushroom (Flammulina velutipes), oyster mushroom (Pleurotus ostreatus), young oyster mushroom (Pleurotus ostreatus), king oyster mushroom (Pleurotus eryngii), button mushroom (Agaricus bisporus), shiitake (Lentinula edodes), and wood ear mushroom (Auricularia auricula-judae). Mushrooms which were not exposed to UV light contained negligible amount of ergocalciferol in all kinds of tested mushrooms, but UV irradiation increased their content of ergocalciferol. Of UV A, B and C, UV B light was the most effective to increase ergocalciferol contents. In mushrooms, the increase in ergocalciferol content occurred only in the peel within 1 mm depth from the surface, which was directly exposed to the UV light. Therefore, when fresh whole mushrooms were irradiated with UV light, lily mushroom, the mushroom with a larger surface area compared to volume, such as lily mushroom, was more favorable in producing ergocalciferol. On the other hand when the mushrooms were freeze-dried and cut, the mushrooms with a higher ergosterol, such as king oyster mushroom, shiitake or button mushroom, were more favorable in generating ergocalciferol.