This study estimates the classification criteria which distinguishes the types of omega-3 health functional foods, fish oils and fish oil usages through 13C-NMR spectra and fatty acids contents analysis. The major fatty acids of omega-3, eicosapentaenoic acid (EPA, C20:5) and docosahexaenoic acid (DHA, C22:6) are being analyzed. 10 ethyl ester (EE) forms and 10 triglyceride (TG) forms are the most common types of fish oils for 20 omega-3 products. Gas chromatography (GC) analysis generally shows the matching EPA and DHA contents of the products listed on the notation. But EE form contents of EPA and DHA are higher and are more varied than the TG form. Most of the samples of EPA/DHA ratio show different content ratios of indicated on the products when comparing with standards. The 13C-NMR analysis of EPA and DHA on sn-1,3 and sn-2 carbonyl peak position with fish oil triglycerides display whether the reconstituted triglycerides (rTG) are being confirmed or not. As a result of the 9 TG form, the 10 TG products showed similar values: EPA sn-1, 3; 13.46~15.66, sn-2; 3.00~4.52, DHA sn-1, 3; 2.43~4.40, sn-2; 3.84~6.36. But one product showed lower contents (EPA: sn-1, 3; 5.88, sn-2; 2.86, DHA sn-1, 3; 2.29, sn-2; 5.95) of EPA, thus it can be considered a different type of oil and only matched six products according to the label. This study is intended to provide basic materials which identify the status for the types and quality of omega-3 fish oil products according to fatty acids profiles and the 13C-NMR spectrum confirmed the location specificity of EPA and DHA.

Our objective in this study is to assess the safety of docosahexaenoic acid (DHA) fortified milk of dairy cows fed feeds containing protected fish oil treated with formaldehyde by analyzing formaldehyde concentration in commercial milk and DHA fortified milk of dairy cows fed formaldehyde treated feed. There are 3 milk samples in this study: Commercial milk (CM), DHA fortified milk for Kid (DHA-K) and DHA fortified milk for Baby (DHA-B). We confirm the fresh quality of these three samples by physicochemical tests. In fat content result, three groups are significantly different at the p < 0.05 by Duncan's multiple range test, but fat content of group DHA-K is about half the level of the other two groups. Protein content of group DHA-K is 1% higher than other two groups. According to the analysis result of DHA content of DHA fortified milk, DHA content of DHA-B is two-fold higher than DHA-K. Similar pattern was seen in the intake based on age. According to HPLC analysis result of formaldehyde concentration in milk, commercial milk and DHA fortified milk are between 0.013 ppm and 0.057 ppm which is formaldehyde standard level in fresh milk settled in WHO (World Health Organization). Three groups have no significantly differences at the p < 0.05 by Duncan’s multiple range test. For this reason, it can be concluded that there is no transition of formaldehyde from dairy cows fed formaldehyde treated feeds to its produced milk. Safety about formaldehyde of DHA fortified milk of dairy cows fed formaldehyde treated feeds is considered similar to commercial milk.

This study was conducted on the properties of exhaust emissions of diesel oil and fish oil blended with diesel oils using a direct injection diesel engine at different loads, and on the conditions of carbon deposits of diesel oil and 40% blend oil in the combustion chamber after 20 hours operation at 1]2 load. The properties of exhaust emissions by fish oil blended with diesel oils showed no significant difference with diesel oil. However, soot emissions decreased, increasing the ratio of fish oil. Carbon deposits by fish oil blended with diesel oils were high level compared with diesel oil, which might be overcome by preheating of fuel oil and operating conditions.

It was tested the possibility that EPA and DHA could be separated from fish oil fatty acid ethyl ester(FAEE) in the supercritical carbon dioxide rectification method. Experiments were carried out in the 1800mm tall and temperature gradient packed rectification column at the pressure of 100bar and carbon dioxide flow rate of 52.43NL/min. Experimental results showed that this method was useful to separate the FAEE by the relative volatility of the components. The maximum attainable concentration of EPA, DHA and both of them in product were 41%, 43% and 57% respectively in this rectification column using raw fish oil feed.

Separation of EPA and DHA from fish oil fatty acid ethyl ester (FAFE) by urea adductive crystallization method was carried out in the supercritical carbon dioxide (SC CO2) as a solvent. Our results showed that SC CO2 is a good candidate as a solvent in the urea adductive crystallization to separate FAFE by the number of unsaturated bonds. Compared to the separation process using methanol. SC CO2 yielded better performance in the overall selectivity of EPA and DHA. The effect of process variables on separation of EPA and DHA was discussed in detailed. A hybrid technology of SC CO2 fractionation and urea adductive crystallization with SC CO2 was conformed as a viable process to separate and concentrate EPA and DHA from fish oil.

The combustion characteristics of diesel oil and fish oil blended with diesel oils were investigated at various blending rate of fish oil in diesel engine and constant volume combustion bomb. The evaporation and combustion duration of diesel oil and fish oil blended with diesel oils were respectively different high and low temperature. The dependence of ignition delay on the temperature was different in high and low temperature ranges which were divided at the 773K. The ignition delay become longer than that of diesel oil as the blending rate of fish oil increases, and its difference were larger at different loads. The densityof smoke was lower as the blending rate of fish oil increases, and the rate offuel consumption showed no significant difference between diesel oil and fish oil blended with diesel oils.

Plasma cholesterol함량은 n6 LA에 의한 영향은 없었으며 n3 LL와 EPA의 순으로 감소되어 plasma cholesterol의 저하는 EFA가 가장 효과적이었다. Plasma TG함량은 CO, PO, FO군에서 모두 낮았으며 특히 PO, FO군에서 유의성있게 감소되었다. n6 LA의 경우 plasma와 간조직의 TG함량은 n6 LA에 의해 감소되었으나. lipogenic enzyme활성도는 변화가 있었다. n3 LL를 투여한 PO군의 경우 plasma와 간의 TG함량은 n6 LA에서와 마찬가지로 감소된 반면, lipogenic enzyme활성은 다소 증가되었으나 간의 TG합성을 증가시킬 정도는 아니었다. n3 EPA에 의해 plasma TG는 BT군에 비해 유의성있게 감소되었으며 lipogenic enzyme활성이 낮아졌음에도 불구하고 간의 TG함량은 BT군에 비해 변화가 없었다. Plasma와 간조직의 지방산 조성은 식이지방산에 의한 영향이 잘 나타났는데 CO군은 C_18:2와 C_20:4의 양, C_20:4/C_20:5의 비율이 높았다. PO군은 C_18:3과 C_20:5, C_22:6의 양이 증가되어 C_20:4/C_20:5의 비율이 남았으며 FO군에서는 C_20:5와 C_22:6양이 가장 높게 나타났으며 따라서 C_20:4/C_20:5의 비율은 가장 낮았다.

The combustion characteristics, ignition delay, p-t, dp/dt, Q-t of diesel oil and fish oil blended diesel oils was investigated according to pressure and temperature in a constant volume combustion bomb. The results are as follows: 1) The influence of temperature and pressure on the ignition delay was almost constant in high temperature, regardless of the blending rates, and the ignition delay was shortest in the 60% blend. 2) The maximum pressure was high in order of with pure diesel oil, with the 20% blend and the 60% blend. 3) The rate of pressure rise was high in order of with pure diesel oil, with the 20% blend and the 60% blend. The rate of maximum pressure rise was significantly higher with pure diesel oil than with two blends. 4) The amount of accumulative heat release was large in order of with pure diesel oil, with the 20% blend and the 60% blend.

In order to understand and explain the spary combustion, it was necessary to understand the disintegration mechanisms of liquid jet. To understand the disintegration mechanisms of liquid jet, the disintegration phenomena and uniform droplet production regions of testing liquid jet was investigated by means of longitudinally vibration capillary nozzle, which was injected the testing liquids. The testing liquids were light oil and light oil and light oil blended with 25wt% fish oil (File fish oil, Sardine fish oil, Alaska pollac oil) The results can be summarized as follows: 1. The uniform droplet phenomena have been changed according to the frequency of capillary tube, the jet velocity and physical properties of testing oils. 2. Within the region of uniform droplet, Reynold number was increased as Weber number increases. 3. The lower limit of wave length in which uniform droplet was produced 0.8d which was lower than Rayleigh wave length 4. The light oil blended with file fish oil which has lower viscosity and surface tension had the widest uniform droplet production region on the frequency and velocity among testing oils. But light oil blended with sardine fish oil were similar with light oil in the uniform production region.

In this paper, combustion characteristics and engine performance varying with blending rate of fish oil using five test fuels, e.g.pure diesel oil and four types of sardine-oil-blended diesel oils, their blending rates by weight being 20%, 40%, 60% and 80% respectively, and operating condition of engine, were investigated experimentally both in the constant volume combustion bomb and in the engine. The results are summarized as follows: 1) In the bomb, the influence of temperature on ignition delay of sardine-oil-blended diesel oils was larger than that of pure diesel oil, and it tended to increase as the blending rate of fish oil increase sardine-oil-blended diesel oils. As far as the influence of pressure on ignition delay concerns, there was no significant difference with all the test fuels. 2) In the engine, the ignition delay of fish-oil- blended diesel oils was longer than that of pure diesel oil, and it tended to increase as the blending rate increases. In the bomb, the ignition delay in high temperature showed no significant difference between with pure diesel oil and with fish-oil-blended diesel oils, and it was especially short with 60% fish-oil-blended diesel oil. In low temperature, however, the delay became longer as the blending rate increase. 3) The combustion duration was shorter with fish-oil-blended diesel oils than with pure diesel oil and it became a little shorter as the blending rate increases. 4) The rate of fuel consumption showed no significant difference between with fish-oil-blended diesel oils and with prue diesel oil, although calorific value of fish oil was lower than that of diesel oil. 5) Smoke density in exhaust gas was lower with fish-oil-blended diesel oils than with pure diesel oil and the higher the blending rate was, the lower the smoke density became

In this paper, to percuss whether fish oil can substitute for marine fuel oil, the characteristics on the evaporation and ignition of 3 fish oils, Sardine oil, File fish oil and Alaska pollac oil, were investigated experimentally by suspending single fish oil droplel in hot atmosphere, and experiments on methanol and light oil were also carried out to compare the characteristics. The results abtained are summarized as follow; 1) Evaporation and ignition phenomena on the methanol and light oil by the present experimental method agreeded with the results of the earlier investigation. 2) The characteristic on evaporation and ignition of all 3 fish oils took the same pattern; in late stage of evaporation at atmospheric Temperature 550℃ droplet rapidly expanded and contracted, and then remained solid corbide, but in case of 650℃ rapidly expanded and ignitied, and then completly burned non-remained solid carbide. 3) As fish oil mixed with light oil (50% weight), in beginning stage of evaporation droplet depended on the characteristics of light oil, but in end stage depended on fish oil. 4) Ignition temperature of fish oil droplets was about 470℃, higher than about 250℃ of light oil, but atmospheric temperature to ignite droplet was about 650℃, lower than about 750℃ of light oil.

본 연구에서는 DHA-enriched fish oil 과 대두유를 비율(w/w)을 달리하여 반응시키고 그 특성을 알아보았다. 어유의 비율이 증가할수록 trilinolein(LLL) peak는 감소하였고, 새로운 피크의 형성은 점차 증가하였다. 재구성 지질(어유 : 대두유 = 5 : 5, w/w, , 240, 180 rpm)의 지방산 조성 분석 결과 대두유에서 볼 수 없었던 DHA가 약 13% 증가한 반면, linoleic acid는 24.17%

식물 추출물들의 어유에 대한 항산화력을 rancimart test, 과산화물가, TBA, DPPH 소거능을 이용하여 알아보고자 하였다. 44종 식물추출물을 1,000 ppm 첨가한 어유를 선 실험하여 대조구보다 induction period가 약 배 증가된 결과를 보인 추출물 7종을 선별하여 주된 실험 시료로 사용하였다. 농도별(100, 200, 300, 500, 700, 1,000 ppm) rancimat test 결과 감자 추출물이 가장 연장

어유에 catechin, 로즈마리 추출물 (Antox1, Antox2, Antox3), carotene, 녹차 추출물 (Gtox) 등을 첨가하여 각각의 항산화력을 rancimat을 이용하여 측정하였으며, synergist외의 상승효과를 측정하였다. 사용된 항산화제 6종 중에서 catechin 500ppm을 첨가한 시료의 induction periods time이 4.35 hr로 control의 0.65 hr보다 3.7 hr의 차이를 보이면서 크게