환경오염과 자원 고갈 문제가 심화됨에 따라 재생 가능하고 생분해가 가능한 바이오소재 개발이 지속 가능한 산업 발전의 핵심 과제로 대두되고 있다. 본 연구는 동아시아 지역에서 자생하는 꽝꽝나무(Ilex crenata Thunb.)를 대상으로, 발아 증진 및 순화재배 기술을 체계적으로 정립하여 대량 생산 체계를 구축하고 바이오소재로서의 산업적 활용 가능성을 평가하고자 하였다. 본 연구에서는 GA3, KNO3, NH4NO3, KH2PO4, PEG와 같은 다양한 프라이밍 처리제를 이용하여 꽝꽝나무 종자의 발아 특성과 유묘 생육 특성을 분석하였다. 그 결과, KNO3 50 mM 처리구는 발아율을 99.0%까지 증가시키며 발아 과정을 가속화하는 데 효과적이었다. 그러나 유묘 생육 및 단위면적당 수확량에서는 NH4NO3 200 mM 처리구가 가장 높은 증가를 보였으며, 이는 질소의 두 가지 형태(NH4 +, NO3 -)를 동시에 제공하여 질소 대사를 극대화한 결과로 판단된다. 유묘활력지수(SVI) 또한 NH4NO3 200 mM 처리구에서 254.3±41.6으로 가장 높게 나타났으며, 이는 대량 생산과 바이오소재 원료로의 활용에 적합한 조건으로 확인되었다. 본 연구는 발아 증진 기술 및 초기 생육 안정화를 통해 꽝꽝나무의 대량 재배 가능성을 제시하였으며, 지속 가능한 그린바이오소재 개발에 중요한 기초자료를 제공하였다. 향후 다양한 재배 조건과 종자 특성에 따른 최적화 연구가 추가적으로 필요할 것으로 판단된다.
Wheat (Triticum aestivum L.), a significant cereal crop from the Gramineae family, serves as a vital source of protein, essential minerals, B-group vitamins, and dietary fiber. However, its productivity is often hindered by issues such as poor seed germination, which can adversely affect yield and crop quality. This study investigated the effects of different silicon concentrations and priming durations on wheat germination and seedling growth. Analysis of variance revealed that silicon treatment significantly influenced key parameters of germination and growth, including germination percentage (GP), germination index (GI), vigor index (VI), radicle length (RL), plumule length (PL), and seedling dry weight (SDW). Priming with silicon at a concentration of 1 mM resulted in notable improvements, increasing GP, GI, VI, RL, and PL by 10.6%, 65.5%, 29.4%, 18.6%, and 28.6%, respectively, after 6 hours of priming. Certain germination traits demonstrated strong positive correlations, particularly GP and GI (r = 0.96) and VI and RL (r = 0.94), after 4 hours of priming. These improvements in seed germination and seedling development may result from enhanced water uptake, stimulated cell division, and increased hydrolytic enzyme activity, which facilitate the mobilization of seed reserves and accelerate the growth of embryonic tissues.
본 연구는 페튜니아(Petunia×hybrida)의 내건성 증진을 위 해 스트레스 메모리 기작을 활용한 프라이밍 처리의 효과를 구명 하고자 하였다. 건조-회복 과정을 통한 프라이밍 처리를 4회 반복 수행하였고, 두 가지 프라이밍 강도(Priming1:물 100mL, Priming2: 물 50mL 관수 후 건조)를 적용한 후 5일 동안 건조스 트레스 조건에서 내성 증진 효과를 관찰하였다. 프라이밍 강도가 높은 Priming2 처리구에서 대조군에 비해 수명이 약 25시간 연장되었다. 이는 페튜니아가 스트레스 메모리 기작을 통해 건조 스트레스에 대한 내성을 높일 수 있음을 보여준다. 본 연구는 정원 식물의 환경 스트레스 내성을 증진시키기 위한 프라이밍 기술의 유용성을 확인하였으며, 향후 다양한 환경 스트레스 조건 에서의 프라이밍 효과를 평가함으로써 화훼식물의 내건성 증진 에 대한 실용적 기술 개발에 기여할 수 있을 것으로 기대된다.
본 연구는 제비동자꽃의 대량번식을 위한 효과적인 발아 조건을 선발하고자 수행되었다. 제비동자꽃 종자는 저온습윤 처리(5°C, 0, 4, 8, 12, 16주), GA3 처리(0, 500, 1000mg・L-1), 프라이밍 처리(0.1M, 0.2M의 NH4NO3, K2HPO4, KCl, KNO3)를 하였다. 모든 종자는 25°C, 12시간 광조건(40μmol・m-2 ・s-1)에 서 발아 실험이 진행되었으며, GA3 처리구는 비닐온실(평균 23.4°C)에서도 비교실험이 진행되었다. 저온습윤 8주 처리구 에서 20.4%로 가장 높은 발아율을 나타냈으며, 12주 이상의 저온습윤 처리는 오히려 제비동자꽃의 발아율을 감소시켰다. GA3 0, 500, 1000mg・L-1 처리된 종자는 25°C에서 각각 3.3, 44.0, 62.0%, 비닐온실에서 각각 5.3, 16.7, 42% 발아하였다. 제비동자꽃 종자는 GA3 농도가 높아질수록 발아율이 증가하였다. 또한 제비동자꽃 종자는 0.2M KNO3-프라이밍 처리구에서 가장 높은 발아율(54.7%)을 나타냈으며, 평균발아소요일수는 처리 간 유의성을 보이지 않았다. 이상의 결과로 효과적인 제비종자꽃 종자 번식을 위한 전처리 조건으로 24시간 GA3 1000mg・L-1 침지 처리 또는 0.2M-KNO3 프라이밍 처리를 추천한다.
While Ardisia crenata Sims. recently has been used increasingly as an ornamental plant, It is endangered in natural forest. The optimum treatments of the various concentrations priming agents to improve seed viability and germination of A. crenata (ornamental plant) were also estimated. Germinability was significantly when the seeds of A. crenata was soaked in PEG solution at 15oC for 4 days, the optimum treatment for improving germination of A. crenata was observed when the tested seeds was soaked in −0.5 MPa of PEG solution in 92%. Overall results would be useful means for propagation and production of A. crenata.
Young Su Kwon. 2017. Gender Semantic Priming Effects on the Lexical Meaning. Studies in Modern Grammar 96, 129-148. Regarding the role of automatic processes in human thoughts and behavior in social psychology, a number of studies have demonstrated that automatic priming effects of ‘gender’ can occur even when the primary meaning for the words does not include the direct gender-related meaning. The purpose of this research has three aspects: (1) to examine whether automatic priming effects on the ‘male’ and ‘female’ social categories will occur with Korean EFL university students, (2) to find out if there are any differences of the priming effects between gender stereotype words and gender suffix words, and (3) to investigate how Korean EFL university students show the rating of the gender typicality of the prime words. As a result, the significant priming effects were observed on the ‘gender’ social category, which were more saliant with the suffix type than the stereotype primes. Also, the rating of the gender typicality showed some attributes related to the sociocultural factors.
This study was conducted to improve the postharvest storage techniques of managing and storing seeds, totest qualities and viabilities of the seeds and to examine the germination rate and the protein expression of Achyranthesjaponica Nakai. The seeds collected from different areas of Je-Cheon and Gwang-Ju were stored with different temperaturesand durations. Two plant growth regulators and two seed priming were treated to investigate their effect on the germinationrates and the days required for germination. The weight of one hundred seed collected in Gwang-Ju was heavier than thosein Je-Cheon. Seed length collected in Gwang-Ju was also longer about 5.12㎜ than those in Je-Cheon about 4.90㎜ andseed width was longer in Gwang-Ju than those in Je-Cheon. The rates of seed germination in two different collection areaswere higher about 2.9 to 13.0% in Gwang-Ju compared to those in Je-Cheon. Comparing its rates with the storing tempera-tures and durations, they were not clearly different in between 4℃ and 25℃ and they also were gradually decreased withgetting longer storing durations. The germination rates treated by plant growth regulators were higher with GA3 than thosewith Kinetin. The highest seed germination rate was appeared at 50 ppm of GA3. Comparing its rates with different seedpriming, they were relatively higher with KNO3 than those with PEG6000. In protein expression patterns between before thegerminating and after the germinating of seeds, more and clear bands were appeared in the seed after the germination com-pared to those before the germination of seeds, especially 10~20kDa. These results showing more and clear bands weremore clearly appeared in Gwang-Ju compared to Je-Cheon. Comparing the protein expression with plant growth regulatorsand seed primings, GA3 was better expression than those with Kinetin and KNO3 was better than those with PEG6000. Moreand clear bands were closely related to the germination rates of seeds and more detailed studies would be required.
This study was conducted to investigate the quality of seeds, the germination rates and the days required for germination, to examine the patterns of protein expressions during the germination and to improve the techniques of managing and storing seeds and viability of the seeds of Lithospermum erythrorhizon Sieb. et Zucc. After collecting and harvesting seeds, they were classified to white and brown colors of seed coat through testing their seed size, weight, and quality. The germination rates, the days required for germination, and the protein expressions were examined with different colors of seed coats, storing temperatures and durations by treating the different plant growth regulators and primings. One hundred seed weight of white color was heavier about 1.17 g than those of brown one about 0.81 g. The germination rates in white color of seed coat was higher, 3.05 ~ 5.75%, than those in brown one. Its rates were decreased with getting longer in storage durations. There was no big differences on germination rates between storage temperatures. The plant growth regulator of GA3 and Kinetin was affected to improve the seed germination. GA3 increased the seed germination clearly at 25 ppm level, while kinetin increased it gradually from 25 to 100 ppm levels. In germination by seed primings, PEG6000 made higher germination rate with increasing their levels, whereas KNO3 increased the germination until 100 mM level and then decreased it with 200 mM unlike PEG6000. The protein expressed during the seed germination were appeared more and clearer bands in the seed after germination, especially 20 ~ 30 kDa, compared to those in the seed before germination. These results showing more and clearer bands were positively related to the germination rates which were different by seed colors, storage temperatures and durations, and plant growth regulators and primings.
This study was carried out to select new pelleting binder and material for seeds from Fraxinus rhynchophylla Hance and Alnus sibirica Fisch. ex Turcz. The optimum treatments of the various concentrations and species of priming agents to improve seed germination of both woody medicinal plants were also estimated. Germinability was increased when the seeds of Fraxinus rhynchophylla Hance was soaked in -1.0 MPa of PEG6000 solution at 15℃ for 4 days significantly, the optimum treatment for improving germination of Alnus sibirica Fisch. ex Turcz was observed when the tested seeds was soaked in 100 mM of KCl at 15℃ for 4 days. The influence of physical and chemical properties of pelleting solid materials, the mixture of gypsum, diatomaceous earth, dalma ceramic and vermicuolite (6:1:1:1 ratio) were found as the best pelleting materials for Fraxinus rhynchophylla Hance and Alnus sibirica Fisch. ex Turcz. seeds. To satisfy the requirements of absorption and compatibility for multi-layer seed pelleting, SGPA (Starch-grafted cross-linked polyacrylates) hydrogel was prepared using starch, acrylonitrile, ceric ammonium nitrate, nitric acid, methyl alcohol and potassium hydroxide. The resulting SGPA hydrogel showed high water absorption but not plant compatibility. It suggested that seed pelleting using pelleting materials and SGPA hydrogel (multi-layer coating) after priming agent treatment is to increase germinability and seedling growth and it can reduced irrigation labours and can also save seed.
자연상태에서 발아율이 불량하고 발아기간이 오래 걸리는 둥굴레의 종자번식을 위해 저장기간을 달리한 종자를 이용하여 종자수명 및 발아적온을 알아보았다. 또한 발아율을 높이기 위해 여러 생장조절제 및 무기염류를 프라이밍처리하였다. 저장기간별 둥굴레 종자의 발아실험의 결과 1~2년까지는 발아율이 떨어지지 않았으나, 4년간 저장하는 경우에는 발아율이 현저히 떨어졌다. 둥굴레 종자의 발아는 고온보다는 22~25℃의 온도에서 양호하였으며, 발아속도도 빨랐다. 특히 1~2년 저장한 종자를 25℃에서 발아시키는 경우 70~71.2% 정도의 비교적 높은 발아율을 보였다. 둥굴레 종자의 발아를 촉진시키기 위하여 여러 종류의 생장조절제와 무기염류를 사용하여 프라이밍처리를 한 결과, BA를 제외한 모든 처리구(GA3, IAA, NAA, kinetin, KNO3, KH2PO4, Ca(NO3)2)에서 무처리보다 높은 발아율을 보여 프라이밍처리 효과가 인정되었다. 특히 1년 저장종자에서 GA3 0.5mM 처리는 96%, IAA 1mM 처리는 93%의 높은 발아율을 보여 가장 발아율을 높였으며, 발아소요기간 또한 단축시켰다.