본 연구는 대한민국 울릉도 특산식물인 추산쑥부쟁이(Aster × chusanensis Y.S.Lim, Hyun, Y.D.Kim & H.C.Shin)의 기내 대량증식체계 확립을 위해 시토키닌 계열 식물생장조절 제를 첨가하여 캘러스 및 신초 형성 조건을 구명하고자 수행 하였다. 실험결과, 추산쑥부쟁이의 줄기 절편체에서 캘러스 와 신초가 형성되었다. 캘러스 형성률은 TDZ 3.0mg·L-1처 리구에서 88.9%로 가장 높았고 BAP 3.0mg·L-1와 Zeatin 3.0mg·L-1 처리구는 66.7%, 2iP 3.0mg·L-1 처리구에서는 11.1%였다. 신초 형성률은 모든 처리구에서 77% 이상이고 특히 TDZ처리구와 Zeatin 처리구에서 100%였다. 그러나 TDZ처리구에서는 형성된 신초가 부풀어 오르거나, 잎에 과수 성이 나타나는 등 형태적 기형이 나타났다. Zeatin처리구에 서 발생한 신초는 정상적인 형태를 나타났다. 잎 절편체에서 는 캘러스, 신초가 발생하지 않았으며 절편체가 탈색하며 고 사하는 것을 확인하였다. 종합적으로 추산쑥부쟁이의 캘러 스 및 신초 형성을 위해서는 줄기 절편체를 배양 재료로 이 용하는 것이 효과적으로 확인되었다. 캘러스 유도에는 TDZ 3.0mg·L-1처리가 가장 효과적이었고, 추후 NAA와 같은 옥신 을 함께 첨가하여 증식 효과를 분석하는 연구가 필요할 것이 다. 또한, 신초 유도에도 TDZ 3.0mg·L-1 처리가 효과적이었 으나 장기간, 고농도로 사용할 경우 형태적인 기형이 발생할 수 있어 정상적인 식물생산에 부적합한 것으로 확인되었다. 따라서 형태적 기형이 발생하지 않은 아데닌 유도체형 시토키 닌인 Zeatin을 활용하는 것을 추천한다. 본 연구는 울릉도 특 산식물인 추산쑥부쟁이의 기내 대량증식과 생태계 유지에 필 요한 종 공급을 위한 대량증식 체계 구축을 위한 자료로 활용 할 수 있을 것이다.

Aster 속에 속하는 자생식물로는 눈갯쑥부쟁이, 개쑥부쟁이, 쑥부쟁이, 벌개미취, 참취 등이 있는데, 대부분 식용 또는 관상용 으로 많이 활용되고 있다. 특히 눈갯쑥부쟁이는 제주 한라산(표 고 1,200~1500m근처)에서 자생하는 한반도 특산식물로써, 다른 Aster 속과 다르게 포복성을 가지고 있고 개체당 소화의 수가 많아 관상가치가 매우 뛰어나다. 또한 파종 당해년에도 개화가 가능하기 때문에 유망한 관상식물 자원이라 할 수 있다. 본 연구 에서는, 한반도 특산 눈갯쑥부쟁이의 유전자원 보존 및 대량번 식을 위한 기초자료를 확보하고자 종자의 발아특성 및 휴면유형 을 분류하였다. 눈갯쑥부쟁이 종자는 형태적으로 완전하게 발달 된 배를 가지고 있고, 배양 72시간 내에 145%의 수분을 흡수하 였다. 4가지 온도조건(4°C, 15/6°C, 20/10°C, 25/15°C)에 배양한 결과, 광조건에서는 각각 67.0%, 58.9%, 62.2%, 71.6% 발아하였 고, 암조건에서는 각각 79.4%, 65.9%, 65.9%, 49.1% 발아하였 다. 저온층적처리(4°C) 실험 결과, 최종 발아율에는 큰 차이가 없었으나, 층적처리 기간 동안에도 발아하는 특성을 관찰하였 다. GA3처리 후 25/15°C에 배양한 결과, 0, 10, 100, 1000mg·L-1 처리에서 각각 57.9%, 68.3%, 74.9%, 63.9%의 발아율을 보였 다. 15/6°C에 배양한 경우에는 각각 78.3%, 62.8%, 72.2%, 55.9% 발아하였다. 본 연구에서는 위 실험들을 통하여, 약 80% 정도는 non-dormant 종자로, 나머지 약 20%는 생리적 휴면 (physiological dormancy)을 가지고 있는 것으로 판단되었다.

Aster koraiensis Nakai (A. koraiensis) which has been used as a food and medicinal plant in the past, is valuable as functional food material. Therefore, the aim of this study was to determine the antioxidant properties and major phenolics of A. koraiensis extracts with different ethanol concentrations (0, 50, 70, and 100% aqueous ethanol solution). When ethanol concentration in the extraction solvent was increased, extraction yield decreased; 34.2, 23.2, 21.0, and 5.5% in 0, 50, 70, and 100% ethanolic extracts, respectively. Total phenolics content and antioxidant activities of extracts were increased in an ethanol concentration-dependant manner. The major phenolics in the extracts were chlorogenic acid (21.264~58.666 mg/g), isochlorogenic acid A (10.432~145.353 mg/g), and isochlorogenic acid C(0.239~13.148 mg/g), and these phenolic contents were higher in 70 and 100% ethanolic extracts than other extracts. Significant correlations were observed between ethanol concentration of extraction solvent, antioxidant properties, and major phenolics. These results indicated that the optimal ethanol concentration for extraction was 70%.

This paper provides basic data for product developers by investigating the consumption status, consumer perception, and consumer demand on products using Aster Yomena. Two hundred ninety-five people over 20 years of age were analyzed according to gender and age. In the purchase of Aster Yomena products, men were the higher purchasers except for powder. In age, noodles, wild vegetables, jangajji, tteok, and powder were consumed by those older than 30 years. In an analysis of purchase when developing Aster Yomena products, the male purchase intention was higher in all items except for kalguksu in the staple, and male purchase intention was higher in only bibimbapseasoning among aster powder addition sauce and all items in dessert. The purchase intention of those older than 30 years was higher in all items of staples, aster powder addition sause and sause The purchase intention of those older than 30 yearse was higher in green vegetables juice and milk tea among drink, and all items except ice cream among dessert. An analysis of the popularization of Aster Yomena revealed higher perceptions of men in the medium (TV, internet, online cafe, agro-fishery market), experience program (area tourism, exposition), and product development (convenience food, recipe development). The medium (agro-fishery market), experience program, and product development were more recognized in their 30s or more. The products with aster yomana require the most effective marketing to men 30 years and older, and require a strategy that will interest those in their 20s.

This study investigated the volatile flavor composition of essential oils from Chrysanthemum zawadskii var. latilobum Kitamura and Aster yomena Makino. The essential oils obtained by the hydrodistillation extraction method from the aerial parts of the plants were analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). One hundred (95.04%) volatile flavor compounds were identified in the essential oil from the C. zawadskii var. latilobum Kitamura. The major compounds were valencene (10.82%), δ-cadinol (9.77%), hexadecanoic acid (8.70%), 2-methyl-4-(2,6,6-trimethylcyclohex-1-enyl) but-2-en-1-ol (3.67%), and 2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4,4]non-3-ene (3.57%). Ninety-eight (93.83%) volatile flavor compounds were identified in the essential oil from the Aster yomena Makino. The major compounds were and 3-eicosyne (13.61%), 9,10,12-octadecatrienoic acid (7.8%), α-caryophyllene alcohol (6.83%), 9-octadecynoic acid (6.03%), and α-caryophyllene (5.74%). Although the two plants are apparently very similar, the chemical composition of the essential oils was significantly different in quality and quantity. In the case of C. zawadskii var. latilobum Kitamura, the sesquiterpene, valencene was found to be 10.82%, but it was not identified in A. yomena Makino. δ-Cadinol appeared higher in C. zawadskii var. latilobum Kitamura than in A. yomena Makino. A clear characteristic of A. yomena Makino essential oil is that it has a high content of caryophyllene derivatives. The α-caryophyllene alcohol contained in A. yomena Makino was relatively high at 6.83%, although the compound was not identified in C. zawadskii var. latilobum Kitamura. Also α-caryophyllene was shown to be higher in A. yomena Makino than in C. zawadskii var. latilobum Kitamura.

This study investigated the effects of blanching time (1-5 min), types of blanching solution (1% NaCl vs 1-3% sucrose), addition of packaging with blanching solution, and thawing methods (thawing in packaged versus depackaged units) on the qualities of frozen Aster scaber. As quality parameters, drip loss, moisture content, shear force, color, and sensory test were conducted. Aster scaber was blanched in 1% sucrose for 4 min and packaged with the same solution prior to freezing whereas frozen Aster scaber exhibited palatable quality when it was removed from the package and thawed in running water. Consequently, this study demonstrated that the optimized processing condition provided qualities like non-frozen control.

This study investigated to find optimal blanching conditions for pre-treatment of Korean Cahmchi (Aster scaber) before freezing to maintain qualities as food ingredient. Blanched A. scaber were blanched in water, 1% sucrose solution and 1% salt solution for 2min. After blanching, each sample packed with water, 0.5% and 1% sucrose solution, which were half quantity of sample. The samples were then frozen at -30°C freezer for 24h. Frozen samples were thawed in water at 4°C. Color, thawing loss, water content and texture analysis were evaluated. Water content was not significantly different among samples. Thawing loss of A. scaber was low value in sugar and salt additive samples. Sugar additive sample indicated the lowest redness and yellowness value. Also sugar added samples showed tender texture. In conclusion, sugar contents in blanching water were affected to qualities of A. scaber.

Aster glehni Franchet et Schmidt is a compositae plant and has been known as a native specie in Ulleung Island, Korea. It is officially recognized as a regional specialty that grows only in this region. In 2014, brown and dark spots were observed on aster leaves in a forest research field, Jinju, Korea. A causal agent was isolated from the disease symptomatic leaves and identified as fungus Alternaria solani. Fungal morphological characteristics and molecular identification with internal transcribed spacer sequences were synchronized as A. solani. The isolated fungi reproduced the same disease symptoms when the fungus was artificially inoculated on healthy aster leaves. This is the first report that A. solani caused leaf blight disease in Aster glehni in Korea.

ASW0 is a polysaccharide derived from the perennial herb Aster scaber Thunberg. We isolated ASW0, a fraction of crude polysaccharide, by means of ethanol precipitation and dialysis after hot water extraction to investigate its physicochemical properties and immunostimulatory effects. ASW0 contains neutral sugar (45.7%), acidic sugar (51.6%), protein (2.3%), and 2-keto-3-deoxy-D-manno-octonate (KDO) (0.4%). The neutral sugar in ASW0 (in mole percentage) was mainly composed of arabinose (34.5 mol%), glucose (31.1 mol%), galactose (14.9 mol%), and rhamnose (8.1 mol%), which are characteristic of pectic polysaccharides. ASW0 also contained small amounts of xylose, mannose, and fucose. The anti-complementary activity of ASW-0 was similar to that of polysaccharide K (used as positive control). ASW0 exhibited no cytotoxicity in RAW 264.7 macrophages and dramatically increased nitric oxide (NO) production in a dose dependent manner (0.3~30 μg/ mL). Also, macrophages stimulated with ASW0 showed enhanced production of immunostimulatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in a dose dependent manner. These results suggest that the ASW0 have a potent immunostimulatory effect and can be used as a natural immune health ingredient.

ASW0 is a polysaccharide derived from the perennial herb Aster scaber Thunberg. We isolated ASW0, a fraction of crude polysaccharide, by means of ethanol precipitation and dialysis after hot water extraction to investigate its physicochemical properties and immunostimulatory effects. ASW0 contains neutral sugar (45.7%), acidic sugar (51.6%), protein (2.3%), and 2-keto-3-deoxy-D-manno-octonate (KDO) (0.4%). The neutral sugar in ASW0 (in mole percentage) was mainly composed of arabinose (34.5 mol%), glucose (31.1 mol%), galactose (14.9 mol%), and rhamnose (8.1 mol%), which are characteristic of pectic polysaccharides. ASW0 also contained small amounts of xylose, mannose, and fucose. The anti-complementary activity of ASW-0 was similar to that of polysaccharide K (used as positive control). ASW0 exhibited no cytotoxicity in RAW 264.7 macrophages and dramatically increased nitric oxide (NO) production in a dose dependent manner (0.3~30 μg/ mL). Also, macrophages stimulated with ASW0 showed enhanced production of immunostimulatory cytokines such as interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in a dose dependent manner. These results suggest that the ASW0 have a potent immunostimulatory effect and can be used as a natural immune health ingredient

본 연구는 지구온난화와 식물 생장의 제한요소로 알려져 있는 질소가 단양쑥부쟁이의 생육에 어떤 영향을 미치는지 알아보기 위해 지구온난화 조건하에서 질소의 농도를 다르 게 처리하여 생육시킨 후 그 결과를 비교하였다. 이를 위하 여 온실을 두 구획으로 나누고, 한 곳은 CO2를 처리하지 않은 대조구로, 다른 한 곳은 CO2를 처리한 처리구로 설정 하였다. 대조구는 대기 중 CO2 농도와 온도를 그대로 반영 하였고, 처리구는 대조구보다 CO2 농도와 온도를 더 높게 상승시켰다. 그 후, 대조구와 처리구 내에서 각각 개체수(1, 2, 3 개체)와 질소량(0, 2, 20g)을 다르게 처리하여 지상부, 잎 폭 그리고 잎 길이에 차이를 비교하였다. 그 결과 개체수 가 하나 이거나 두 개일때, 대조구와 처리구 모두 질소량이 증가할 수록 줄기길이, 잎 길이 그리고 잎 폭 모두 좁아졌다. 개체수가 세 개일 때, 질소량이 증가할 수록 대조구는 줄기 길이가 낮아졌고, 처리구는 줄기길이, 잎 길이 그리고 잎 폭 모두 짧아졌다. 일반적으로 단양쑥부쟁이는 하천변에서 군락을 이루어 서식하는 것으로 알려져 있는데, 이 결과는 지구온난화는 거의 영향이 없고, 하천 오염이 심해질 경우 단양쑥부쟁이의 생육에 피해를 입힐 수 있을 것으로 판단된 다.

지구온난화 조건에 따른 단양쑥부쟁이(Aster altaicus var. uchiyamae)의 생태적 반응을 알아보기 위해 2012년 5월부터 2012년 11월까지 실험하였다. 유리온실 안을 두 구획으로 나눠 대조구(온도 약 2℃상승, CO₂농도 약 340ppm) 와 온난화 처리구(온도 약 3.5℃상승, CO₂농도 약 470ppm)로 나누었다. 그리고 각각 유기물(0%, 1%, 5%, 10%)과 수분(100ml, 300ml, 500ml, 700ml)을 4구배씩 처 리하여 양묘하였다. 그 후 2년생 단양쑥부쟁이를 수확하여 지상부 길이, 줄기 두께, 줄기 무게, 잎 수, 잎 무게, 지하부 길이, 지하부 무게, 종자 수와 종자 무게를 측정하였다. 유기 물에 대한 생태적 지위폭은 대조구에서 0.943, 온난화 처리 구에서 0.924였다. 수분에 대한 생태적 지위폭은 대조구에 서 0.954, 온난화처리구에서 0.929였다. 유기물과 수분에 대한 생태적 지위폭 모두 대조구가 온난화 처리구보다 더 넓게 나타났다. 이러한 결과는 지구온난화가 되면 단양쑥부 쟁이는 생태적 지위가 감소함을 의미한다. 2년생으로 알려 져 있는 단양쑥부쟁이가 대조구와 처리구에서 2년째 생식 생장을 하지 않고, 이듬해인 3년째 계속해서 영양생장을 하 고 있는 개체들이 관찰되었다. 3년생 단양쑥부쟁이 비율은 온난화처리구(58.5%)에서 대조구(38.0%)보다 높았다. 3년 생 단양쑥부쟁이의 비율은 대조구에서 유기물이 많을수록 높게 나타났다. 그러나 온난화처리구에서는 대조구의 결과 와는 다르게 유기물 함량이 많을 때 그 비율은 가장 낮았다. 수분 구배 처리에서는 유기물 구배 처리의 결과와는 다르게 수분이 가장 많은 조건에서 비율이 가장 높거나(69%,온난 화처리구), 가장 낮았다(30.8%,대조구).

This study investigated the antioxidative activities and quality characteristics of Aster scaber Bibimbap as a home meal replacement with blanching (BASB) or without blanching (ASB) pretreatment. BASB samples at 5.0 g showed higher moisture, crude protein, and ash contents than the control (p<0.05). Color L, a, b-values of samples significantly decreased with increased content of Aster scaber powder (p<0.05). BASB samples showed lower hardness than ASB samples. BASB samples showed higher total phenolic content and DPPH radical scavenging activity than ASB samples. BASB samples showed higher sensory values for color, savory aroma, savory flavor, and aftertaste than ASB samples. Except for glossiness, BASB samples showed higher sensory acceptance scores than ASB samples. Sensory acceptance tests of all samples showed high scores for ‘usually like’, representing the possibility of successful development of Bibimbap as a home meal replacement, especially that containing blanching pre-treated Aster scaber powder.