Barley breeding program of RDA (Rural Development Administration) has been develop to new hulled barley variety that has been used for malt and beer material. Through this program, we developed new two-rowed covered barley, Imaeg, with disease resistant, lodging tolerant and good malt quality for beer and Korean traditional drink ‘Sikhye’ in 2013. Imaeg showed resistant to Barley yellow mosaic virus (BaYMV), lodging tolerant and early maturing. The regional yield trials were conducted for Imaeg as a breeding line ‘Iksan166’ during three years at four different regions. And then it was registered to Plant protection right, No. 5681 in 2015 after field experiment by KSVS (Korea Seed & Variety Service). Imaeg has I of vernalization and shorter stem length as 77cm than 7cm to control variety Hopum. Yield potential of Imaeg about 4.8ton/ha that higher about 3% in paddy field condition than control, however, it has no significant difference. In quality examination for beer usage, Imaeg has higher 1,000 grain weight (45.6g) and higher assortment ratio (93.6%) than those of Hopum as 42.6g and 90.4% respectively. In malt quality, Imaeg showed higher soluble protein, Kolbach index and diastatic power than those of Hopum significantly. In addition, Imaeg has better ‘Sikhye’ malt quality significantly in lightness of grain (58.4 L value) and growth rate (216.8%) than those of Hopum as 54.3 (L value) and 177.5% respectively. Imaeg would be suitable for cultivation at the area above -4°C of daily minimum temperature in January in Korean peninsula.

Barley yellow mosaic virus (BaYMV) is transmitted by a root-inhabiting Polymyxa graminis (P. graminis) and thus the disease is called “soil-borne”. In this study, the presence of P. graminis was confirmed by PCR test based on specific sequences of P. graminis type Ⅰ (P. graminis f. sp. temperata) Internal Transcribed Spacer (ITS). P. graminis was detected in the BaYMV infected soil and root of barley plants. The monitoring of P. graminis was conducted in March 2015 in 8 korean provinces. It was detected in the soil of all collected regions. This is the first report on a P. graminis a survey of Korea.

Improvement of winter hardiness, lodging resistance and good quality have been recently received more attention by covered barley(Hordeum vulgare L.) breeders than ever in Korea. ‘Hyeyang’, a new covered barley cultivar with early maturing and high yield was developed by the National Institute of Crop Science, RDA in 2010. It was derived from the cross between‘Milyang87’and‘Wolsung8120’with good quality. The initial cross was done in 1999 and the selected line, ‘Iksan432’ (YB4494-B-B-21-2), showed high yield and good quality characteristics under yield trial test from 2008 to 2010. The following is the characteristics of‘Hyeyang’that is characterized as the vernalization of Ⅰ, green leaf, compact spike and long rough awns. The heading date of‘Hyeyang’were similar to‘Olbori’. The culm length was 82 cm which was 6 cm shorter than‘Olbori’. It showed the spike length of 4.3 cm, 798 spikes / m2, 56 grains / spike and 26.7 g for 1,000 grains weight. It showed similar winter hardiness and stronger resistance to barley yellow mosaic virus (BaYMV) compare to‘Olbori’. It showed higher diastatic power 235DP than that of check cultivar, ‘Olbori’. Average yield of ‘Hyeyang’ was 4.44 MT/ha in the regional yield trials carried out in four location at double cropping system in Korea from 2008 to 2010, which was 8 percent higher than ‘Olbori’. ‘Hyeyang’would be suitable for the area above –8℃ of daily minimum temperature in January in Korean peninsula.

‘다혜’는 답리작 적응 조숙 내재해 다수성 등을 목표로 조 숙 대립 특성을 지닌 ‘수원318호’를 모본으로, 보리호위축병 에 강한 ‘수원311호’를 부본으로 인공교배 육성 선발된 ‘익산 409호이다. 파성은 Ⅲ형이며, 이삭의 밀도가 조밀한 편이며 까락이 길지만 탈망성이 좋다. 출수기와 성숙기는 각각 4일 빠른 조숙종이다. 흰가루병은 ‘올보리’와 비슷하며, 보리호위 축병에 대한 저항성을 나타냈다. 내한성과 내도복성은 ‘올보 리’보다 다소 강하였다. 간장(88 cm)과 수장은 ‘올보리’와 비 슷하였으며, m 2당 수수는 666개로 ‘올보리’보다 7개 적고, 1 수립수는 52개로 2개 많으나 통계적인 차이는 없었다. 천립 중은 ‘올보리’와 비슷한 대립종이며, 1 L중은 다소 가벼웠다. 단백질 함량, β-glucan 함량과 정맥률은 비슷하였고, 입백도 는 약간 높았다. 엿기름의 효소력가는 ‘올보리’보다 25 DP 높았다. 수량은 전작에서 4.00 MT/ha, 답리작에서 4.11 MT/ha 이었다.

A New six-rowed Naked Barley Cultivar “Nurichal” with good coking quality was developed by cross with HB88154-B-25-1 line and Hinchalssalbory by the Winter Cereal and Forage Crop Research Div. NICS, RDA in 2010. HB15305-B-B-31-2 was selected in 2006 and designated as ‘Iksan 95’. It showed good agronomic performance in the regional yield trials (RYT) from 2008 to 2010 and was released with the name of ‘Nurichal’ possessing good water absorption and expansion. The average heading and maturing dates of ‘Nurichal’ were April 24 and May 30 in paddy field, which were two day and one day later than those of the check cultivar ‘Saechalssalbori’ respectively. The new cultivar, ‘Nurichal’, had 82cm of culm length that was 5cm shorter than that of ‘Saechalssalbori’, and 4.0cm of spike length. It showed 774 spikes per m2, 63 grains per spike, 25.0 g of 1,000-grain weight, and 793 g of test weight. ‘Nurichal’ showed a little weaker winter hardiness than those of the check cultivar, ‘Saechalssalbori’ and showed similar lodging resistance with check cultivar. It sowed stronger resistance to barley yellow mosaic virus (BaYMV) compare to check cultivar. Grain shape was small and round. Protein content was 10.3% and β-glucan content was 6.8%. Its average yield of the pearled grain in the regional yield trial was 3.84 MT/ha in paddy field, which were higher 2% than those of the check cultivar. Its water absorption was similar and expansion was higher than ‘Saechalssalbori’. This cultivar would be suitable for the area above the daily minimum temperature of -6℃ in January in Korean peninsula.

Improvement of winter hardiness, diseases resistance and good quality have been recently received more attention by covered barley(Hordeum vulgare L.) breeders than ever in Korea. “Hyegang”, a new covered barley cultivar with similar maturing and high yield was developed by the National Institute of Crop Science, RDA in 2011. It was derived from the cross between “Milyang87/NWX-GB-G 2” and “Milyang87/Chalbori” with good quality. The initial cross was done in 2000 and the selected line, “Iksan445” (YB4625-B-B-6-2), showed high yield and good quality characteristics under yield trial test in 2008. It was cultivated three times for three years (2009～2011) in the four locations of regional yield trial (RYT) in korea and was investigated about agronomic and quality characteristics and released as “Hyegang”. The heading date was May 4 in upland and April 26 in paddy field which was 1 and 0 day lateness respectively than that of check cultivar, “Olbori”. The culm length was 77 cm which was 6 cm shorter than that with check cultivar. It showed the spike length of 4.7 cm, 800 spikes / m2, 64 grains / spike and 25.1g for 1,000 grains weight. It showed similar maturing and stronger resistance to barley yellow mosaic virus (BaYMV) compare to check cultivar. It showed higher diastatic power 216DP than that of check cultivar, ‘Olbori’. Average yield of “Hyegang” in the RYT was 4.61 MT/ha in upland and 4.38 MT/ha in paddy field, espectively.

"다호"은 맥주보리 고품질 내재해성 신품종을 개발하기 위하여 1997년에 작물과학원에서 대립이면서 색택이 양호한 밀양 85호를 모본으로 하고 바이러스저항성인 수원 335호를 부본으로 인공교배1 하여 육성한 품종으로 2007년 12월에 농작물 직무육성 신품종 선정 위원회에서 "다호"이라 명명하여 맥주보리 호위축병저항성 중간모본으로 국가품종보호출원 하고 그 특성은 다음과 같다. 다호는 2조형 맥주보리로서 출수기와 성숙기가 빠른 중생종이며 호위축병은 진주,

"오름"은 맥주보리 고품질 내재해성 신품종을 개발하기 위하여 1997년에 작물과학원 에서 보리 호위축병 저항성인 'Kinuyudaka'를 모본으로 하고 단간이며 내도복성인 '삼도보리'를 부본으로 인공교배하여 육성한 품종으로, 2006년 12월에 농촌진흥청 농작물 직무육성 신품종 선정 위원회에서 "오름"이라 명명하여 국가품종목록에 신품종으로 등록하였으며 그 특성은 다음과 같았다. "오름"은 2조형 맥주보리로서 출수기와 성숙기가 빠른 중생종이고 간장은 75

Barley Yellow Mosaic Virus (BaYMV) caused significant reduction in barley yield and is difficult to control due to alive parasitic soil-borne fungus, Palmyra gamines that transmits the, virus. Previous studies have indicated that a virus-free soil could be infested by using virus-contaminated farming machineneries and implements. For the further confirmation of this finding, different proportions of BaYMV-infested soil were mixed into virus free soil. Three barley varieties (Hordum vulgarae, cv "Olbori", "Baegdong" and "Sacheon 6") were sown in pots treated with different rate of P. graminis-infested soil ranging from 0% to 100% in October 20, 2001. Results showed that BaYMV infection increased as the rate of infested soil increased. Initial symptoms were observed in a pots treated with 10% infested soil in all the 3 varieties of barley. "Olbori" had about 5% infection in 20% infested soil and about 10% infection in 40% or 50% infested soil and about 20% infection in 60% infested soil. In "Baegdong", the trend of BYMV occurrence was similar with "Olbori" but the time of severe infection was earlier than "Olbori". BaYMV infection in "Sacheon 6" was even earlier than "Baegdong" with much more severe symptoms than "Baegdong". The growth rate of barley was affected by about 19-22% when grown in 20% infested soil. As the rate of BaYMV infested soil increased the heading date was delayed but the maturing date was early in "Olbori" and "Sacheon 6". Also, reduction rate of culm length in 3 varieties increased with increase of infested soil content. However, "Olbori" showed the highest reduction. "Sacheon 6", have been characterized with long spike length, however was significantly reduced as the infested soil increased. On the other hand, spike length of "Olbori" was not significantly affected despite of increased of infested soil. The reduction rate of 1000 kernel weight was higher in large kernel size cultivar "Sacheon 6" and "Olbori" than small kernel size "Baegdong" as increase of BaYMV-infested soil content.

A new whole crop silage barley cultivar, “oungyang” was developed from the cross between “unong and Milyang55 at the Yeongnam Agricultural Research Institute (YARI) in 2002. An elite line, YB3882-3B-17-1-3-1, was selected in 1999 and designated as “ilyang111” It showed good agronomic performance in the regional adaptation yield trials (RYT) from 2000 to 2002 and was released by the name of “oungyang”with higher yield and better animal feed quality than that of Olbori. The average heading and maturing dates of Youngyang were May 2 and June 6, which were late by 2 & 1 days than those of Olbori, respectively, in Suwon. Youngyang had longer culm length, 83cm but it had lesser spikes per m2 637 than that of Olbori, respectively, in RYT from 2000 to 2002. The rate of leaf sheath/whole length (culm+leaf) and the rate of the grains/ whole crop weight was similar to Olbori. It showed resistance to BaYMV at the regions of Naju, Jinju, Milyang andt Iksan. however, the response of Youngyang to other environmental stresses (tolerance of cold and wet) was similar to Olbori. The dried whole crop yield potential of Youngyang in the RYT was about 11.67 MT/ha in paddy fields which was about 18% higher than that of Olbori. The grain yield potential was 6.32 MT/ha in paddy fields which was about 5% higher than that of Olbori. Youngyang had lower rate of shattering, higher content of protein and NDF, lower ADF and TDN, and better quality of silage than those of Olbori.

It was practiced that crop rotation between barley and soybean to reduce the Barley yellow mosaic virus(BaYMV) in barley and investigated barley growth and yield. The diseased degree of barley by BaYMV reduced in one or two years fallow of barley with the soybean cultivated continuously in summer season. In the same practices, barley growth such as plant height and tiller number was better than another crop rotation plots. In yield components of barley, culm length and number of head was different among the treat plots. One or two years fallow of barley plots was better results than others. The yield of barley also showed significant difference in above one or two year fallow of barley. These plots showed higher yield from 24% to 32% than continuously cultivated barley and soybean plot. In comparison to conventional practice barley and rice, these rotation system presented higher barley yield from 3% to 13%. These crop rotations affect the density of the BaYMV vector, Polymyxa graminis. In these results, crop rotation with one or two years fallow of barley in viral infested paddy fields could reduce the BaYMV disease incidence and fungal vector density in soil.

보리의 저항성 정도에 따른 바이러스병에 의한 생육 및 수량 피해를 조사하였다. 1. 병징 발생정도에 따라 백동과 새쌀보리가 각각 7~9 와 5~7 의 이병정도를 보여 감수성과 중도저항성을, 내한쌀보리는 0~1 정도로 저항성으로 조사되었다. 2. 감수성과 중도저항성 품종에서 Barley yellow mosaic virus와 Barley mild mosaic virus의 감염이 확인되었으나, 저항성인 내한쌀보리는 바이러스 감염이 검정되지 않았다. 3. 월동 후 생육 신장 조사에서 저항성은 지속적인 생육 증가 현상을 나타낸 반면, 중도저항성과 감수성 품종은 10.5~11.8cm 신장이 억제되는 생육 피해를 보였다. 4. 수량구성 요소의 피해를 건전포장 결과와 비교했을 때 중도저항성과 감수성 품종에서 저항성에 비해 출수기, 수수, 간장 등에서 특히 차이가 큰 것으로 나타났다. 감수성 품종에서 출수기는 7일 늦어졌고, 간장과 수수는 50% 이하의 생육을 보였으며, 중도저항성에서는 수수와 간장의 생육이 일반 포장에 비해 20% 정도 억제되는 피해를 보였다. 5. 품종별 저항성 정도에 따른 수량 피해 정도를 조사 결과, 병 발생이 없는 일반포장에 비해 중도저항성과 감수성 품종에서 10a당 108~288kg 감소되는 피해를 보인 것으로 조사되었다. 6. 바이러스 감염시 중도저항성인 새쌀보리는 평균 35%의 수량 감소 피해를 보였으며, 감수성인 백동의 경우 평균 63%의 수량 감소 피해를 나타내었다.

A new malting barley variety, Hopum, was developed from the cross between Sacheon 6 and Misato Golden at thenated as Milyang114. It showed good agronomic performance in the regional adaptation yield trials (RYT) from 2001 to 2003 andwas released with the

Suwon295 at the Honam Agricultural Research Institute (HARI) in 2005. An elite line, 953017-BG-BN-BN-53N, was selected in2000 and designated as Milyang126. It showed good agronomic performance in the regional adaptation yield trials (RYT) from2003 to 2005

월동 후 맥류 재배지에서 나타나는 이상 증상으로는 주로 잎에 황화등의 변색과 모자이크성 반점 등이 조사되었다. 이들 증상을 가진 잎의 바이러스 검정 결과 78~% 이상에서 바이러스 감염이 확인되었으며, 주로 BaYMV와 BaMMV에 의해 발생하였다. 전국일원의 맥류 재배지에서 4개년간 BaYMV, BaMMV SBWMV와 BYDV-MAV(2003년) 등 4종의 바이러스의 발생율을 조사한 결과 대상 바이러스 중 BaYMV가 가장 높은 감염율을 나타내었다. BaYMV는 조사 4개년 동안 평균 발생율이 70~% 이상으로 전국적으로 큰 차이 없이 가장 높은 발생율을 보였다. 그러나 경기지역의 경우는 20~% 정도로 다른 지역의 65~~85~% 에 비해 낮은 발생율을 보였다. BaMMV는 전북, 전남, 경기, 강원, 경남지역에서 20~~40~% 를 보인 반면, 경북, 충남, 경기지역에서는 발생이 적었다. SBWMV와 BYDV-MAV는 현재까지 국내 보리 재배지에서 다발생되고 있지는 않았다. 저항성 유전자원에 대해 바이러스에 대한 저항성 반응을 검정한 결과 BaYMV와 BaMMV가 단독 또는 복합 감염되는 형태로 나타났으며, SBWMV는 감염이 확인되지 않았다. 저항성 유전자별 반응을 검정한 결과에서 익산 발생하고 있는 BaYMV-Ik strain과 BaMMV에 대해 모두 저항성인 유전자는 확인되지 않았으나 국내 맥류 재배지에서 가장 발생이 많은 BaYMY-lk체 대해 저항성 반응을 보인 유전자는 Ishukushirazu, Chosen에 들어있는 rym 3, Tokushima Mochi Hadaka의 rym 4y 및 Hakei I-41의 rym 5a로 나타났다. 그러나 BaYMY-lk에 대해 저항성으로 확인된 rym 3, rym 4y 및 rym 5a의 경우 BaMMV에는 모두 감염이 확인되었으며, rym 1+5 두개의 저항성 유전자를 가지고 있으며 일본의 모든 BaYMV strain에 저항성인 Mokusekko 3도 감염이 확인되었다. 유전자에 따른 병징 발생 양상을 조사한 결과 일반적인 바이러스 병징과 같이 대부분 모자이크나 황화가 발생하였다. 그러나 저항성 유전자에 따라 고사, 괴사 반점, 조직 괴사, 줄무늬성 증상과 잎 말림등의 다양한 증상이 확인되었다

보리호위축병 피해지역에 대한 수량감소의 예측을 위하여, 보리호위축병(BaYMV)에 대한 저항성 품종과 이병성 품종간 수량구성요소, 수량 및 증질 변이를 건전구와 이병구에서 비교 조사하였다. 1. 보리호위축병의 이병주는 BaYMV에 감염된 것으로 나타났으며, 이병정도는 감수성 품종인 백동에서 9, 저항성 품종인 내한쌀보리에서 1정도 발병하였다. 2. 감수성 품종인 백동은 천립중을 제외한 간장, 수당립수, m2 당 수수 등의 수량구성요소가 유의하게 감소하였으며, 수량은 대조구의 20%수준으로 현저히 감소하였다. 3. 보리호위축병 감염구에서 종자의 발아율은 감수성 품종과 저항성 품종간에 차이가 없었으나, 정립률은 감수성 품종인 백동에서 설립중이 유의하게 증가하였으며, 대조구에 비하여 정립률과 조단백질 함량 모두 유의한 차이를 보였다. 4. 보리호위축병은 간장, 수량, 정립률, 수수, 천립중, 1수립수 등의 피해와 부의 상관을, 조단백질 함량과는 정의 상관을 보여 보리호위축병에 이병이 되면 수량 및 품질에 크게 피해를 주는 것으로 나타났다.

1. 4개년 조사 기간 중 보리호위축병 발병율은 6.6%-62.5%로 연차간 기상조건에 따라 큰 변이를 나타내었다. 2. 면역혈청학적 검정 결과 보리호위축병의 감염이 확인되었으며, 일부 품종은 BaMMV와의 혼합 감염이 발생하였으나 보리호위축병에 비해 상대적으로 발생이 낮은 경향이었다. 3. 월동전 보리 초기 생육 기간의 기상 조건과 발병율의 변화 조사 결과 평균기온이 바이러스의 감염과 유의성 있는 상관을 나타내었다. 4. 월동기중에는 기상조건과 발병율과는 어떤 요인도 유의성 있는 상관을 나타내지 않았다. 5. 생육재생기 이후의 발병율은 최고기온과 유의성 있는 상관을 나타내어 병징 발현에는 최고기온이 영향을 미치는 것으로 조사되었다.