In resent year there has been research on benefits of conjugated linoleic acid (CLA) and docosahexaenoic acid (DHA) for human health. The effect of a fat supplement made from seaweeds meal plus palm olein meal (FS) and replacement of concentrate on milk production, and milk fatty acid (FA) profiles of dairy cows fed grass silage based diets was evaluated. Ten Holstein lactating cows were divided into a two groups. The cows in a group were fed concentrate at 40% on TDN basis (control). The cows in another group (Treatment) were supplemented 0.5㎏/day/cow of FS as a substitute for 2㎏/day/cow of concentrate. The medium chain fatty acids and DHA content of FS were 345 ㎎/g DM and 35 ㎎/g DM, respectively. Total DMI, DM digestibility and milk production as well as milk composition did not differ between the two groups. The intake of ether extract was higher (P＜0.01) in Treatment than control group (1.1 ㎏/day/cow vs. 1.3 ㎏/day/cow). The short and medium chain of FA in the milk did not differ between two groups. The trans11C18:1vaccenic acid, cis9trans11CLA and C18:3 concentrations in the milk were higher (P＜0.01) in Treatment than control group(1.88 g/100gFA vs. 1.03 g/100gFA, 0.93 g/100gFA vs. 0.51 g/100gFA and 0.48 g/100gFA vs. 0.48 g/100gFA). However, the C18:2 concentration in the milk was lower (P＜0.05) in Treatment than Control group (1.38 g/100gFA vs.1.17 g/100gFA). There were detected 0.13 g/100gFA of DHA in the milk in Treatment group. Results indicated that addition of the FS has the potential of increasing CLA and DHA in cow's milk.

A full genomic DNA microarray technique was employed to investigate the effects of Dongchunghacho on aortal and hepatic gene expression in apolipoprotein E knockout mice fed a high-fat/high-cholesterol diet. Male 8- week - old ApoE-/- mice were randomly divided into two groups, control(high cholesterol group; HC) and supplementation of Dongchunghacho (SD). All of the mice were fed a high-fet/high cholesterol diet with or without Dongchunghacho supplemented by 1% for 6 weeks. At first, lipid profile of the Dongchunghacho was measured by biochemical analysis. No differences were observed in serum triglyceride and total cholesterol levels between the two groups. Antigenotoxic effect of the Dongchunghacho was measured by the single cell gel electrophoresis assay (Comet assay) and quantified as % fluorescence in tail. Dongchunghacho supplementation decreased significantly leukocytic DNA damage and also there was a tendency of reduction in hepatic DNA damage in Dongchunghacho group compared with the control group. In up regulated genes in liver and aorta of the mice, genes with 0 to 2- fold difference in expression level between the two group (HD and SD) was very much more in liver than in aorta, on the contrary, those with 2-fold to 16-flod difference increased greatly rather in aorta than in liver. Also, almost the same results were observed in down regulated genes in liver and aorta between the two groups. These results suggested that supplementation of Dongchunghacho might be helpful in preventing leukocytic DNA damage induced by high fat diet, and has a more crucial roles in aortal gene expression.

In order to determine whether green tea tannin ameliorates abnormal arginine metabolism as the result of excessive arginine, we have assessed the effects of the administration of green tea tannin mixture in rats treated 30 days with 2% arginine. In the arginine-treated group, the level of guanidino compounds such as arginine (Arg), guanidinoacetic acid (GAA), creatinine (Cr), methylguanidine (MG) and guanidinosuccinic acid (GSA), nitric oxide, urea, protein and glucose increased significantly in the serum, urine and kidney, whereas the oxygen species-scavenging enzymes of kidney were reduced as compared with the non-argmme-treated group. By way of contrast, the administration of green tea tannin reduced blood urea nitrogen and serum creatinine, and reduced the urinary excretion of guanidinoacetic acid, creatinine, and NO2-+NO3-. The increased levels of urinary urea, protein and glucose in the arginine-treated group were also lowered by the administration of green tea tannin. In these groups, the activities of superoxide dismutase and catalase in the kidneywere increased, thereby suggesting the involvement of radicals in the normalizing of kidney function. These results show that the abnormal renal function induced by the adminstration of excessive arginine in rats may be restored by treatment with green tea tannin.

본 연구는 제주도와 인근 부속도서에 서식하는 까치의 번식 밀도와 계절별 식이물을 조사하기 위하여 이루어졌다. 번식 밀도를 알아보기 위한 둥지 분포 조사는 까치의 번식기인 2008년 2월부터 4월까지, 식이물 조사는 2006년 5월부터 2008년 2월까지 이루어졌다. 조사결과, 제주도 전역에서 관찰된 둥지 수는 모두 2,113개로 둥지의 평균 밀도는 1.33개/km2였으며, 해발 600여 미터까지 분포하는 것으로 나타났다. 둥지 밀도는 제주시 중부지역이 688개소, 3.61개/km2로 가장 높은 반면, 제주시 동부지역이 214개소, 0.66개/km2로 가장 낮은 밀도를 보였다. 해발 고도별 둥지의 밀도는 100m 미만에서 1,172개소, 1.85개/km2로 가장 높았고, 해발 500-600m에서 16개소, 0.20개/km2로 가장 낮게 나타났다. 제주도내 유인도에서 관찰된 둥지 밀도는 비양도 8개소로 15.38개/km2, 우도 9개소로 1.49개/km2, 가파도 1개소로 1.15개/km2였고, 마라도에서는 관찰되지 않았다. 위 내용물은 개체수 파악이 불가능한 뼈, 조류알껍데기, 식물, 종자를 포함하여 모두 17종류였으며, 그 중 딱정벌레류가 가장 많았다. 봄과 여름에는 무척추동물인 곤충류의 포식빈도는 높았지만, 겨울에는 30% 내외로 낮았다. 식물과 종자의 빈도는 봄에 각각 10%와 30%로 낮게 나타났으며, 겨울에는 모두 100%로 가장 높은 빈도를 보였다. 그리고 포식된 택이원 중 빈도는 크게 나타나지 않았지만 조류의 알과 뼈도 관찰되었다. 생태계 내에서 상위 포식자 역할을 하고 있는 까치 개체군 밀도의 증가는 소형 조류나 소형 파충류 등의 토착종의 종수와 개체수의 감소에 직접적으로 영향을 미칠 것이 예상되어 이에 대한 장기 모니터링 및 대책 마련이 필요하다고 판단된다.

The principal objective of this study was to determine the effects of diet knowledge and perception on diet practice behavior among university students. Data were collected between March and April of 2005 from 523 male and female university students in Seoul and Chungbuk, via a self-administered questionnaire. The subjects included 217 male students (41.5%) and 306 female students (58.5%), and 52.0% of the subjects were 21-23 years old. With regard to residence type, 42.6% of the subjects lived in the dormitory, home (29.1%) self-boarding (19.5%) and meal (8.8) were in the order. The average weights and heights of the subjects were as follows: 70.9±9.0 kg and 174.3±4.4 cm in the male students and 53.9±5.9 kg and 161.3±4.5 cm in the female students. The average BMI values of the male and female students were 23.4±2.5 and 20.8±2.1, respectively. The average nutritional knowledge scores were as follows: 1.96 out of 3.0, dietary habit (2.13), nutrition (2.0), exercise (1.98), preferred food (1.90), and chronic disease (1.79). We noted no significant differences between the male and female subjects in terms of scored points. Nutritional knowledge by gender and residence type were shown to be significantly different--the male subjects had higher scores for exercise than did the female subjects. The self-boarding subjects had significantly lower scores with regard to nutritional knowledge and chronic disease. The experience and duration of previous diet practice were also significantly different with regard to gender and residence type. The male subjects tended to conduct diet practice for longer periods than females. The self-boarding students reported more attempts at diet behavior, but for shorter durations. The results show that the nutritional knowledge and diet practice behavior of the subjects were influenced by gender and residence type.

Murgot Looper Moth, Ascotis selenaria, is one of the worldwide pests in citrus plants. They usually damages citrus leaves and often makes young fruits injured. In Jeju, it has been discovered that A. selenaria is a pest of two major geometridae moths being occurred in citrus plant and they also had periodically occurred and damaged bean, asparagus, etc. However, physiological and ecological studies were rarely conducted on this species, which maybe resulted from the limitation of rearing method. Therefore, this study aimed on selecting the diet sources and developing mass-rearing method for A. selenaria, in order to make ease or spur a investigation related with A. selenaria. The major sources, selected among the series of semi-artificial diet, were corn and bean powder and adding yeast makes the larva developed well. Cholesterol, one of the minor sources, enhanced the development rate and lowered the mortality during 1 to 3 larva stage. Consequently the semi-artificial diet, suitable for rearing A. selenaria larva, was composed of 25g agar, 100g corn powder, 100g soybean powder, 30g yeast, 0.5g cholesterol, 2g Vanderzant's vitamin mixture, 2g Wesson's salt mixture, 2g sorbic acid, 2.5g methyl-4-hydroxybenzoate(MPH), 2g Ascorbic acid, and 1000 ml distilled water. A. selenaria larvae has 6 developmental stage and the width of head piece was 0.31 mm (1st instar) , 0.51 mm (2nd), 0.85 mm(3rd), 1.37 mm (4th), 2.14 mm (5th), and 3.19 mm (6th). The development period of A. selenaria, reared at 25℃ and 16L:8D photoperiod, was 4.7 day (egg), 3.58 day(1st instar), 1.88 day (2nd), 1.80 day (3rd), 2.46 day (4th ), 2.59 day (5th), 8.10 day (6th), 11.26 day (pupae) when citrus leaves were supplied to the larva. The larvae, fed with the artificial diet(mentioned above), showed similar developmental periods at each developmental stage as much as the case reared with citrus leaves, although the mortality was sometimes a little bit higher. The weight of pupae were 0.614g (artificial diet) and 0.427g (citrus stuff) and female adult moths lay 1094 eggs (artificial diet) and 1054 eggs (citrus stuff).

본 연구에서는 한식의 해외 현지화를 목표로 take-out형 franchise system 개발을 위한 기초자료를 얻기 위하여 한국적인 특성을 나타내면서 미국인들에게 선호도가 높았던 4 개의 품목을 선정하여 한국식단의 영양적인 우수성을 확인 하고자 실시하였다. 실험기간은 총 9주로서 전반기 5주(실험 1期)는 모두 고지방식을 급여하였고, 후반기 4주(실험 2 期)는 동결건조된 한식(제육볶음, 김치볶음, 두부조림, 나물 무침)을 첨가한 실험식이를 급여하였다. 대조군은 실험 전 기간 동안 고지방식만 급여하였다. 실험종료시 체중은 실험 군간에 유의적인 차이가 없었고, 실험기간 동안 일당 증체 량과 식이효율도 차이가 없었다. 장기무게는 대조군(A)이 한식식이군(B~E)에 비해 낮은 경향이었다. 체지방의 축적 지표로서 내장지방량(RFP, EFP)은 대조군(A)보다 김치볶음군(C)과 두부조림군(D)이 20% 감소하였다. 고지방식을 급여한 후 한식첨가식이를 4주 동안 급여후 혈청 지질 중 TC농도는 나물무침군이 94.13 mg/dL로 실험군중 통계적 으로 유의하게 낮았다(p<0.05). TG농도는 실험군간에 통계 적인 차이가 없었다. 고밀도지단백-콜레스테롤(HDLC) 농 도는 제육볶음군(20.38 mg/dL)이 두부조림군(17.25 mg/ dL) 보다 유의하게 높았고(p<0.05), 김치볶음군과 나물무침군은 차이가 없었다. 저밀도지단백-콜레스테롤(LDLC) 농도는 두부조림군이 가장 높았던 반면 나물무침군이 통계적으로 가장 낮았다(p<0.05). 이상의 결과에서 김치볶음과 두부조림 및 나물무침은 한국적인 특성과 건강기능성을 갖는 한식품목으로서 향후 이들 품목과 제육볶음을 적정하게 응용하여 미국인의 입맛에 맞는 take-out형 음식이 개발된다면 미국인들에게 한식의 영양적인 우수성과 인지도를 높여 널리 보급할 수 있을 것으로 기대된다.

The purpose of the present study was to examine the nutrient intake status of elderly dementia patients. We surveyed the dietary intake of 50 demented elderly patients receiving a regular diet, who were hospitalized in a geriatric hospital in Yongin, Gyeonggi-do. The average age of the subjects was 79.6 土6.5 for the males and 80.5 土6.3 for the females. The average heights were 169.5 土6.0 cm (males) and 154.6 土5.2 cm (females), and the average weights were 58.5 土7.4 (males) and 51.7 土8.9 kg (females). Depending on the type of dementia, the male patients showed significant differences in their intakes of vegetable protein, fiber, total iron, and non-heme iron between the Alzheimer’s disease (AD) and the vascular dementia (VD) groups, and the female patients showed significant differences in their intakes of total protein, phosphorus, zinc, and vitamin B6 between the two groups. The male patients showed significant differences in their intakes of animal-source calcium and carotene according to ability to self-feed. According to physical activity, the male patients showed significant differences in vegetable fat and vitamin A intake, and the females showed significant differences in their intakes of total protein, animal protein, fiber, ash, total calcium, vegetable-source calcium, animal-source calcium, phosphorous, total iron, non-heme iron, heme iron, sodium, vitamins B1, B2, B6, niacin, vitamin C, folate, and potassium. We found that the patients consumed excessive protein, but low amounts of calcium, vitamin B2, and folate. Also, the patients’ physical activity abilities appeared to affect their nutrient intakes.

The effects of Lotus (Nelumbo nucifera Gaertner) leaf dry powder on serum lipid concentrations were evaluated in rats. Forty-eight male Sprague-Dawley rats were divided into six groups and fed high fat diets for sis weeks. Experimental groups were following diets; Control group (CON), 40 % animal and plant high fat group (AHF and PHF) control with 2% Lotus leaf dry powder group (CLDP), animal and plant high fat diets with 2% Lotus leaf powder group (AHFLDP, PHFLDP). Tissue weights of liver, heart, kidney, spleen and lung of AHF group exposed rats were decreased by PHFLDP groups. The concentrations of serum triglyceride in rats fed the CLDP and PHFLDP group were lower than those in other groups. The concentrations of total cholesterol in CLDP and PHFLDP group the were lower than those in AHF groups. The concentrations of HDL-cholesterol in serum of the CLDP and PHFLDP groups were significantly higher than those of other groups. The levels of LDL-cholesterol in serum of the CLDP and PHFLDP groups were tended to be lower than those of other groups. GPT and GOT were decreased in CLDP and PHFLDP groups and than in the AHF group. LDHase was lower in CLDP and PHFLDP groups than in the AHF group. These results suggest that 2% Lotus leaf dry powder groups may reduce elevated levels of serum lipid concentrations in rats fed high fat.

Development of pellet-type artificial diet for insect was tried by using a twin screw extruder(TSE). Screws were arranged several times and two reverse screws were equipped on the foreparts of 2 axes of TSE. Pellet-type diet could be produced successfully under conditions of TSE set as the following. , 300 rpm of main motor speed, 228 rpm of feed motor speed, 75㎖/min of running water speed and 5 ㎜ of extrusion diameter. The optimum adding quantity of water to the manufactured diet was 1.2~1.5 times of dry diet. On the rearing results of beet armyworm, Spodoptera exigua and common cutworm, Spodoptera litura with the pellet-type diet, the final survival ratio (emergence rate) of these two species was over than 50%, and so it was concluded that the manufactured pellet-type artificial diet was sufficient dietetically to rear those insects.

Recently, epidemiological evidence has raised concerns that moderate elevation in body iron stores may increase oxidative stress and the risk of cardiovascular disease and cancer. Onion flesh or peel contains antioxidant such as flavonoids and alk(en)ylcysteine sulphoxides. This study was conducted to examine the effect of onion flesh or peel feeding on antioxidative capacity in aged rats supplemented with high dietary iron. Thirty-two Sprague-Dawley male 16-month-old rats weighing 618±6g were acclimated for 10 days with medium-iron diet (35ppm in diet), and blocked into 4 groups according to their body weights and raised for 3 months on either control diets (adequate iron-35ppm or high iron-350ppm) or experimental diets containing onion flesh/peel (5% w/w in diet) with high iron (350ppm). Rats fed high iron-onion peel diet had significantly high quercetin and isorhamnetin levels in plasma whereas rats fed high iron-onion flesh diet did not show. Plasma TBARS level was lowered by onion flesh or peel diet with high iron supplementation. However, there was no significant difference in cellular DNA damage in brain and kidney tissue among all experimental groups. We concluded that high iron diet (10 times higher than requirement) tend to increase oxidative stress and it is plausible that onion flesh or peel feeding enhances antioxidative capacity in the elderly even with iron supplementation.

To elucidate the effect of feeding differently prepared CaCO3 (industrially purified limestone) on laying performance, egg shell quality and serum calcium and phosphorus concentration, totally 288 layers (52 wks old), with 16 birds per replicate, 3 replicates per treatment for six treatments were employed into the series of CaCO3 treatments followed by feeding study. Six CaCO3 treatment and diet preparation includes mash diet with naturally sized CaCO3 (MNaC), mash diet with coarsely particled CaCO3 (MCoC), mash diet with finely particled CaCO3 (MFiC), pelleted diet with naturally sized CaCO3 (PNaC), pelleted diet with casein coated CaCO3 (PCnC) and pelleted diet with agar coated CaCO3 (PAgC). Hen day egg production of the bird fed both fine CaCO3 and pelleted CaCO3 were lower (p<0.05) than those fed other CaCO3 diets. Although there is no difference in average egg weight among treatments, the weight tended to be heavier once the egg production rate was low. There is no difference in feed conversion ratio (intake/egg) among treatments. Egg shell thickness was the highest (p<0.05) with feeding of MCoC whereas the value was the lowest with feeding of pelleted CaCO3 diet. Although there is some difference in egg shell thickness, the shell breaking forces were not significantly different among treatments. Calcium content of the shell was the lowest (p<0.05) in the egg from the layer fed finely particled CaCO3. Serum Ca content tended to decrease up to 8 hrs after feeding, then tended to increase afterwards. Although both agar and casein coating of CaCO3 tended to retard the recovering increase of blood Ca, the difference needed to be clarified with individual ovulation cycle. Serum P level was generally decreased as time passed after feeding. Crude protein utilizability was the highest with feeding of agar coated CaCO3 and that of fat was higher with hens fed pelleted diets. Both ash and P utilizabilities of diet with finely particled CaCO3 were the highest among treatment although there were no differences in both energy and Ca utilizabilities. Conclusively, both the particle size of CaCO3 and pelleting of CaCO3 diet could affect shell quality of the egg. The effect was more evident by the differentiation of CaCO3 particle size than any other processing such as pelleting and coating.