열대몬순기후인 캄보디아에서 2011년부터 2013년까지 건기와 우기로 구분하여 사료용 옥수수인 국내 품종인 광평옥과 평안옥 그리고 수입 품종인 CP888과 Loeung Mongkul에 대한 생육과 수량특성을 구명한 결과이다. 출사 소요일수는 건기 재배에 있어서 2011과 2012년 및 2013년에는 큰 차이가 나타나지 않았으나, 우기 재배에서는 2012년이 2011년에 비해 6일이 단축되었으며 품종간 약 2-3 일의 차이를 보였다. 종실 수량은 건기 재배에서 2012년(548-788 kg/10a)이 2011 년(386-505 kg/10a)과 2013년(345-687 kg/10a)에 비해 다소 높았다. 우기 재배에서는 2012년(369-582 kg/10a)과 2011년(221-601 kg/10a)간에 차이를 보이지 않았다. 그러나 품종간 유의한 차이를 보였다. 본 연구결과 우기 재배에서 안정적인 옥수수 수량을 확보하려면 강수량이 높은 8월과 9월 및 10월 보다는 우기 전인 6월과 7월에 파종하는 것이 좋으며, 건기 재배에서는 충분한 관개가 있는 조건에서 12월부터 다음 년도인 2월 말까지가 파 종하는 것이 최대 수량을 확보하는데 유리할 것으로 사료된다.
기후 온난화가 우리나라 남부지역에서 쌀 수량 및 미질에 미치는 영향을 예측하기 위해 우리나라와 일본 사이의 지역간 비교시험을 수행한 결과를 요약하면 다음과 같다.
1. 쌀 수량은 익산에서 조생종은 10 a당 495 kg, 중생종은 510 kg, 중만생종은 540 kg이었으며, 하이누즈까에서 조생종은 485 kg, 중생종은 477 kg, 중만생종은 518 kg으로, 익산에 비해 하이누즈까에서 모든 생태형의 수량이 낮았다.
2. 천립중은 익산에서 조생중은 20.2 g, 중생종은 23.6 g, 중만생종은 21.0 g이었다. 쓰꾸바에서 모든 생태형별 천립중은 익산보다 약간 무거웠고, 하이누즈까에서는 조생중 22.8 g, 중생종 25.3 g, 중만생종 23.0 g으로 세 지역 중 천립중이 가장 무거웠으며, 생태형별로는 세 지역 모두 중생종>중만생종>조
생종 순으로 무거운 경향을 보였다.
3. 외관상 미질을 고려한 완전미 쌀 수량은 익산에서 10 a당 조생종 366 kg, 중생종 395 kg, 중만생종 448 kg으로 조생종보다 중만생종의 완전미 수량이 많았다. 반면에, 하이누즈까에서는 조생종 423 kg, 중생종 393 kg, 중만생종 400 kg으로 조생종이 중만생종보다 완전미 수량이 많았다. 이상의 결과를 종합하면, 익산지역이 하이누즈까와 비슷한 수준으로 온난화가 진행된다면 쌀 수량은 2 ~ 6% 정도 감소할
것으로 보이나, 완전미 수량의 경우 생태형 및 품종에 따라서 증가하거나 감소할 수도 있는 것으로 나타났다. 따라서 기후온난화에 대비하여 남부지역에 적응하는 품종을 육성하기 위해서는 감광성보다는 감온성 유전자원을 탐색하여 이용하는 것이 중요하다고 생각되며, 중만생종 중심에서 조생종의 품종 비율을 높이는 방안도 필요할 것으로 사료된다.
육성년대 및 생태형에 따른 품종군별 생육형질과 수량관련 형질, 품질관련형질에 대한 특성의 품종변이와 상관을 분석한 결과는 아래와 같다.
1. 출수일수 및 간장은 2000년대 이후에 육성보급 품종군은 1970년대 이전에 육성 보급된 품종군보다 유의성 있게 단축되었고 통일형 품종군은 육성 년대 어떤 품종군의 일반형 품종군보다 출수일수 및 간장이 짧았고 단축되었다.
2. 2000년이후 최근에 육성 보급한 품종일수록 수량이 증가했으며 이는 수수 및 수당립수증가에 기인한 것으로 판단된다.
3. 최근 육성한 품종일수록 현미 길이는 짧아졌고 폭이 좁은 단원립의 형질 특성을 가졌으며 단백질함량도 낮아졌으며 품질평가치의 개선이 뚜렷하였다.
4. 육성보급 년대에 따른 품종군별 수량에 미치는 영향이 수량구성요소 형질에 따라서 상이하였다.
5. 육성년도에 따른 모든 일반형 품종군과 통일형 품종군에서 품질평가치는 단백질함량 및 아밀로스함량과는 부의 상관을 보였다.
The effects of seeding rate and N fertilizer on leaf area index, dry matter accumulation, grain yield and yield components in oats were studied using split experimental design. The results indicated t㏊t seeding rate and N fertilizer ㏊d significant influence on leaf area index, dry matter accumulation and grain yield. With the increasing seeding and fertilizer rates, leaf area index, aboveground dry matter increased significantly. Oat reached the highest grain yield under 112.5㎏/h㎡ seeding rate, N2 was the best N fertilizer treatment for oat.
Amaranth (Amaranth caudatus) is attracting attention as a preference crop in Gyeongsangbuk-do province. To determine its growth potential and cultivation requirements, we investigated its growth characteristics, dry matter productivity, and grain yield according to the growing period. Growth and dry matter productivity were significantly higher for plants that were sown on May 10th when the temperature was the highest, whereas the yield was significantly higher for plants that were sown on April 10th. Amaranth grain yield ranged from 96 to 243 kg according to the sowing date and cultivation year. The optimum harvest time for plants that were sown on April 10th, May 10th, and June 10th were 120, 110, and 110 days after seeding, respectively. The mean temperature and growing period had a significant quadratic function with yield. Based on these equations, the optimum growing temperature was estimated as 20.6℃ and the optimum growing period as 104-119 days after seeding.
Rice quality should be considered as a primary consumer requirement. Regarding marketing, characteristics such as appearance, physicochemical properties, and palatability of brand rice are of high economic importance. Therefore, this study was conducted to select the optimal rice cultivars representing the commercial rice brands of Gyeongsangbuk-do province in Korea. Various rice grain quality components, grain appearance, several physicochemical properties of rice grain, and texture or palatability of cooked rice grains of ten cultivars (namely ‘top quality rice’) cultivated at three different locations, such as inland mountainous and southern plain areas of Gyeongsangbuk-do province in 2013~2014, were evaluated, and the obtained data was analyzed. ‘Hiami’ showed slightly higher protein contents and lower palatability of cooked rice than the other rice cultivars. Rice production and head rice yield produced at Gumi were the highest. The protein content of milled rice produced at Andong, an inland mountainous region, was approximately 0.3% point lower than that from other locations, whereas the amylose content of milled rice was approximately 1% point higher than those from the other plain regions, Daegu and Gumi. We evaluated the texture, Glossiness value determined using a Toyo teste meter and palatability of cooked rice of ten cultivars. The hardness of cooked rice produced in Andong was slightly lower than that produced in Daegu and Gumi, and additionally, the palatability of cooked rice produced in Andong was the best, followed by that producted in Gumi and Daegu. Considering rice yield and grain quality in the major rice cultivation areas of Gyeongsangbuk-do province, the rice cultivars that may be suitable for each region could be recommended mid-late maturation: ‘Younghojinmi’ and ‘Mipum’ in Daegu, ‘Daebo’, ‘Samgwang’, Chilbo’ and ‘Younghojinmi’ in Gumi, ‘Samgwang’, ‘Jinsumi’ and ‘Sukwang’ in Andong. These results obtained in this study imply that the selected cultivars with high yield and quality could be recommended with high priority to rice farmers in the regions.
Green manure has been used as alternative to chemical fertilizer. To evaluate the effect of green manure on the chemical properties of top-soil and sorghum yield, hairy vetch (Vicia villosa Roth, HV), manure barley (MB), and a mixture of hairy vetch and manure barley (HV+MB) were incorporated into the soil at a rate of 100 kg-N N ha -1 before the sorghum was transplanted. Total biomass of sorghum grown in the HV, MB, and HV+MB treatments was 13.1, 31.6, and 25.2 t ha -1 , respectively, and the nitrogen production of the treatments was 81, 74, and 145 ㎏ ha -1 , respectively. The SPAD value of the uppermost leaf of sorghum plants grown in the soils with HV, MB, or HV+MB were very similar until heading stage; however, at maturity, the SPAD value of sorghum cultivated in the soils with HV was lower than that of sorghum in the soils with MB or HV+MB. This could be because the nitrogen release from HV was too rapid to supply nitrogen to sorghum during the later stage of grain filling. Compared with chemical fertilizers, the incorporation of green manure increased the pH, exchangeable cations (K + , Mg ++ , and Ca ++ ), and total nitrogen in soil postharvest, indicating an improvement in soil chemical properties. Total carbon content increased in soil with green manure incorporated, but decreased in the chemical fertilized soil, suggesting that sorghum cultivation using green manure may sequester carbon in soils. The yield of sorghum cultivated with green manure was not different from the yield of sorghum cultivated with chemical fertilizers. These results suggest that the mixture of hairy vetch and manure barley can be a useful chemical fertilizer alternative in sorghum cultivation.
Post-translational modifications of nucleosomal core histones play important roles in biological processes via altering chromatin structure and creating target sites for proteins acting on chromatin. Molecular genetic studies with Arabidopsis have verified several epigenetic factors that regulate flowering time. However, the roles of chromatin remodeling factors have not been well explored in rice. Here, we identified chromatin remodeling factors, OsVIL1, 2, and 4 (Oryza sativa VIN3-LIKE) genes, that regulate grain yield. OsVIL proteins contain a plant homeodomain (PHD) finger, which is a conserved motif of histone binding proteins. We showed that plant height and number of spikelets per panicle were increased in the OsVIL2-overexpression (OsVIL2-OX) and osvil4 plants, respectively. Each mutants (OsVIL2-OX and osvil4) exhibited longer internodes and thicker stems than wild type controls. Histochemical analysis revealed that cells are smaller in OsVIL2-OX and osvil4 plants. We performed an RNA-seq using 1st internodes of WT and OsVIL2-OX stems and got the suppressed target genes in the OsVIL2-OX. OsCKX2, which encodes cytokinin oxidase/dehydrogenase is one of the suppressed genes in the OX plants and we verified decrease of that gene using qRT-PCR and closed chromatins of OsCKX2 were enriched in the OX plants by using ChIP. As results of these, cytokinins were enriched in the OX plants. These demonstrate that OsVIL2 and OsVIL4 antagonistically regulate plant height and number of spikelets by controlling cytokinin contents. Like OsVIL2-OX and osvil4 plants, besides, OsVIL1-OX plants were also shown increased plant height and biomass. We propose that OsVILs may be used for improving grain yield by increasing biomass.
The Arabidopsis gene AVP1 encodes a vacuolar H+-translocating inorganic pyrophosphatase (EC3.6.1.1) that functions as an electronic proton pump in the vacuolar membrane and affects growth development and stress responses in plants. This study was conducted to evaluate the molecular properties of the A. thaliana vacuolar H+-pyrophosphatase (AVP1) gene in rice. Incorporation and expression of the transgene was confirmed by PCR and quantitative real-time PCR, respectively. Expression of the AVP1 gene in transgenic rice plants (TRP1 and TRP2) resulted in significantly enhanced tolerance to 100 mM NaCl under greenhouse conditions when compared to control wild-type (WT) rice plants. Augmented AVP1 expression in the transgenic rice plants also affected total biomass and improved ion homeostasis through increased accumulation of Na+ ions in whole tissues when compared to control WT rice plants under high salinity conditions. The Fv/Fm values of transgenic rice plants were higher than those of WT rice plants, even though the values decreased over time in both WT and transgenic (TRP1 to TRP8) rice plants. Furthermore, rice grain yield and biomass of the transgenic rice plants were at least 15% higher based on the culm and root weights and panicle and spikelet numbers when compared to those of the WT rice plants during the farming season in Korea. Thus, these results suggest that ectopic AVP1 expression conferred tolerance and stress resistance to genetically modified transgenic crop plants by improving cellular ion homeostasis against salt conditions, which enhanced the rice yield and biomass under natural conditions in paddy fields.
The maize genome is complex with exceeding the levels of intra-specific variation, repetitive DNA content, and allelic content observed between many species. Because of tremendous diversity and variants, maize is considered as a forefront crop development and estimation of molecular markers for agricultural trait in genetics and breeding. Using quantitative trait loci (QTL) and marker assisted breeding (MAS), molecular breeders are able to development of drought tolerance and grain yield in maize genotype. To study QTL congruency, a meta QTL analysis including results from eight-teen QTL publications for grain yield and drought tolerance were considered. Among them, we assembled 420 QTLs for abscisic acid (ABA) concentration, anthesis silking interval (ASI), days to flower, days to silk, ear number, kernel number, grain number and grain yields, involved in drought tolerance and grain yield. The meta QTL analysis revealed significant evidence for linkage of these traits to 39 different segments as candidates regions on maize genome. A total of 571 marker was selected as QTL or integrated QTL markers for narrowing down the QTL region into specific functionally relevant candidates. The results of meta QTL analysis helped to refine the genomic regions of agricultural traits, interest described and provided the closest flanking markers.
A new peanut(Arachis hypogaea ssp. fastigiata L.) variety “Hwaseon(Milyang50)” with the high yield potential was developed at the Department of Functional Crop, NICS, in Milyang in 2013. This was developed from the crossing line between cultivar “Iksan 31” with large grain and high quality and “SP9614” with short stem. “Hwaseon” which is Shinpung plant type has 41cm of main stem length and 10 branch number per plant. Each pod has two grains with long ellipse shape of brown testa and yield components is composed of 45 mature pods of per plant, 84g of 100-seed weight, 79% of pod shelling ratio in the regional yield trials(RYT). Seed quality showed 47.3% of crude oil and 27.6% of protein content. This variety also showed more resistant to early leaf spot, and stem rot, compared with check variety “Daekwang”. In the regional yield trials “Hwaseon” outyielded check variety by 12% with 4.78 MT/ha for kernel.
등숙기 일조부족이 수량 및 수량구성형질, 쌀의 이화학적 특성 및 식미치에 미치는 영향을 분석하고자 자연광을 대비하여 35%, 55% 차광 처리한 결과는 다음과 같다. 1. 등숙기 차광처리에 의해 미품, 호품, 신동진의 등숙률, 현미천립중, 쌀 수량은 감소하였고 차광정도가 클수록 높은 감소율을 보였다. 2. 차광처리시 백미 단백질 함량은 증가하였고 35% 차광보다 55% 차광에서 높았으며, 미품이 호품과 신동진에 비해 단백질 함량의 변화가 적었다. 3. 차광처리시 아밀로스 함량 변화는 없었으나 식미치는 차광정도가 높을수록 감소하였고 미품이 신동진과 호품에 비해 변화가 적었다. 4. 차광에 의해 최고점도, 최저점도, 최종점도 및 강화점도는 낮아지고 호화온도와 치반점도는 높았다.
The use of agricultural by-products as alternative nutrient sources in crop production had gained popularity in order to reducing the rate of chemical fertilizer application in the field. This study was conducted to determine whether the application of rice milling by-products treated with yeast inoculants could substitute, or reduce the rate of chemical fertilizer application. The results of agronomic measurements showed that the effect of incorporated materials was not immediate, as compared to 100% chemical fertilizer application. However, grain yield and quality was either the same or greater than 100% chemical fertilizer application. It was found out that expanded rice hull (treated with yeast or not) could reduce the rate of applying chemical fertilizers by half. Also, yeast treatment was only favorable only to expanded rice hull and not with rice bran, and was already found to be a potential material in reducing chemical fertilizer application in rice production.
The excessive and indiscriminate use of chemical fertilizers in the past has brought serious soil and other environmental problems so alternatives over this agrochemical are being searched. Our study focuses on the effects of expanded rice hull inoculated with selected beneficial microorganisms on growth (through agronomic characters), yield and yield components, and grain quality indices of rice. Results showed that favorable effects of different expanded rice hull preparations were not readily apparent at vegetative stage and only treatments with supplemental chemical fertilizer application were comparable with the conventional practice. Expanded rice hull combined with 50% rate of chemical fertilizer exhibited a significantly higher yield (6,471 kg ha-1) over conventional practice (5,719 kg ha-1). Good milling quality indices were observed in treatments having 50% chemical fertilizers plus alternatives from expanded rice hull. Finally, we demonstrated that chemical fertilizer rate can potentially be reduced into 50% if combined with expanded rice hull, and show even better output than chemical fertilizer alone.
조생종 벼 품종인 오대, 운광과 HR28021-AC16 계통에 대한 벼흰잎마름병 저항성 유전자분석과 K1과 K3a 균계를 접종한 후에 수량 및 품질 관련 형질들의 변이에 대하여 분석하였다. 오대는 저항성 유전자를 보유하지 않는 것으로 나타났고, 운광은 Xa3 유전자를, HR28021-AC16은 Xa21 유전자를가지는 것으로 확인되었다. 오대는 K1과 K3a 균계 모두에 이병성을 보였고, 운광은 K1에는 저항성을 K3a에는 이병성을보였다. HR28021-AC16은 K1 균계에는 이병성을 나타냈고K3a 균계에는 저항성 반응을 보였다. 균계접종에 의한 이병성 정도와 2차 감염 정도로 볼 때, K3a 균계가 K1 균계에 비해 병원성이 강한 것으로 나타났다. K1과 K3a 균계 접종에따른 생산력검정시험에서 균계 처리와 반응에 의해 변이가발생한 형질들 중에 등숙률, 정현비율, 현미수량과 완전미도정수율은 서로 간에 정의 상관관계를 나타냈으며, 이들 형질들과 현미 및 백미 단백질 함량은 부의 상관관계를 나타냈다.사미는 등숙률 및 완전미도정수율과 부의 상관관계를 나타냈다. 주성분분석에서 주성분 1을 기준으로 등숙률, 정현비율,현미수량 및 완전미도정수율과 사미, 현미 및 백미 단백질 함량이 다른 방향성을 나타냈고, 주성분 2를 기준으로는 등숙률과 사미가 다른 방향성을 나타냈다. Xa21 유전자를 가지고있는 HR28021-AC16은 K3a에는 저항성 반응을 보였으나K1 균계 접종구에서는 등숙률, 정현비율, 현미수량 및 완전미도정수율은 감소하였고 사미는 증가하는 등 이병성 반응을나타냈다. Xa21 유전자는 최근 큰 피해를 주는 K3a 균계에대해 강한 저항성을 보이나, 우리나라 우점 균계인 K1에 이병성을 보이기 때문에 K1 균계에 저항성인 다른 유전자와의집적을 통한 활용이 안정적인 저항성 증진에 효과적인 것으로 나타났다.