The aim of this study was to evaluate the probiotic properties of lactic acid bacteria (LAB) isolated from kimchi and antioxidant and anti-inflammatory activities of heat-killed LAB strains. Regarding probiotic properties, Lactiplantibacillus plantarum SMF398 and L. plantarum SMF470 isolated from kimchi can survive under artificial gastric condition and adhere strongly to HT-29 cells. The SMF398 and SMF470 strains showed strong antimicrobial activity and co-aggregation against pathogenic bacteria. The heat-killed cells of LAB (1 mg/mL) were prepared by heating at 121℃ for 15 min. MTT assay revealed that the heat-killed cells (1 mg/mL) of SMF398 and SMF470 were not toxic to HT-29 cells. The heat-killed SMF398 and SMF470 showed significantly higher DPPH and ABTS radical scavenging activities as well as β- carotene bleaching inhibitory activity than the heat-killed L. plantarum ATCC14917, a control probiotic strain (p<0.05). In lipopolysaccharide-induced RAW264.7 cells, the heat-killed SMF398 and SMF470 significantly reduced the nitric oxide production by 30.92% and 22.81%, respectively (p<0.05). Furthermore, the heat-killed SMF398 and SMF470 significantly decreased the gene expression levels of tumor necrosis factor-α, interleukin-6, inducible nitric oxide synthase, cyclooxygenase-2 up to 58.02%, 67.70%, 53.44%, 45.90%, respectively (p<0.05). These results suggest that the heat-killed L. plantarum SMF398 and L. plantarum SMF470 might be useful as antioxidant and anti-inflammatory agents.

In this study, probiotic properties such as acid resistance, bile tolerance, intestinal adhesion and fat accumulation inhibition of lactic acid bacteria isolated from kimchi were investigated in order to develop probiotics effective anti-adipogenic effects. Among the 120 types of lactic acid bacteria isolated from kimchi, 42 types of lactic acid bacteria being to acid resistance, 35 types of lactic acid bacteria being to bile resistance, and 24 types of lactic acid bacteria being to both acid resistance and bile resistance were selected. These 24 types were tested for the intestinal adhesion ability which is an essential factor of probiotics’ function and L. rhamnosus GG was used as a positive control in this test. 10 types of lactic acid bacteria having the intestinal adhesion ability were selected. The selected 10 types of lactic acid bacteria were treated in 3T3-L1 cells and intracellular fat was stained with Oil Red O reagent to measure fat accumulation. As a result, in the group treated with lactic acid bacteria OS-15, 48, 102 and 120, fat accumulation was significantly decreased compared with the non-treated group. And the strain OS-48 showed the greatest inhibitory effect on fat accumulation. The lactic acid bacterium OS-48 was identified as Lactiplantibacillus plantarum by analysis based on 16S rRNA sequencing and was named Lactiplantibacillus plantarum OS-48. Therefore, based on the results of this study, L. plantarum OS-48 is expected to be very useful as probiotics with anti-obesity effect in the food industry such as fermented food and functional food.

This study aimed to investigate the effects of antioxidant and anti-inflammatory activities of heat-killed lactic acid bacteria (LAB) produced under different temperature conditions. Regarding probiotic properties, Limosilactobacillus fermentum SMF743 and Lactiplantibacillus plantarum SMF796 isolated from kimchi showed strong acid and bile salt resistance, adhesion activity onto HT-29 cells, and antimicrobial activity against pathogenic bacteria. Based on the results of thermal death time and temperature, heat-killed LAB cells (1 mg/mL) were prepared by heating at 70oC (180 min), 80oC (120 min), 100oC (30 min), and 121oC (15 min). The heat-killed SMF743 and SMF796 showed significantly higher DPPH and ABTS radical scavenging activities than live cells (p<0.05). The heat-killed SMF743 and SMF796 at 70oC or 121oC revealed stronger DPPH and ABTS radical scavenging activities and inhibition of nitric oxide production than those at 80oC or 100oC. Furthermore, heat-killed SMF743 and SMF796 at 121oC significantly reduced the gene expression levels of tumor necrosis factor-, inducible nitric oxide synthase, and cyclooxygenase- 2 up to 53.33%, 58.67%, and 83.67%, respectively, in lipopolysaccharide (LPS)-induced RAW264.7 cells (p<0.05). These results suggest that heat-killed L. fermentum SMF743 and L. plantarum SMF796 can be used as natural antioxidants and anti-inflammatory agents.

In this study, the quality characteristics of kimchi, such as its salinity, pH, and acidity, were measured and compared, and the HT-29 human colon cancer cells were used to show the anticancer effects of kimchi. The kimchi samples used herein included standard kimchi (SK), turnip kimchi (TK), and turnip-powder-added kimchi (TPK). The measured pH and acidity of TK and TPK showed no significant differences with those of SK. Compared to SK and TK, TPK had higher DPPH scavenging activity and higher total flavonoid content, confirming its antioxidant activity. The cancer cell growth inhibition rates of TK and TPK were significantly higher than that of SK. In HT-29 cells treated with TPK, the mRNA expression of Bcl-xL, an anti-apoptosis-related gene, was lower, and the mRNA expressions of the apoptosis-related genes Bax, Bad, and caspase-9 were higher. TPK showed significantly higher levels of mRNA expressions for the cell-cycle-related genes p53 and p21 than the other samples, in addition to suppression effects on cancer cell proliferation. Compared to SK, TK and TPK suppressed the growth of colon cancer cells and showed higher anticancer effects. Therefore, it is shown that kimchi with added turnip powder had high anticancer effects.

Research on the birth (起源) and names (語源) of foods such as kimchi is important to understand traditional food culture. kimchi, an ‘add flavored, fermented, pickled, vegetable food’ was initially prepared with the simple purpose of increasing storage capabilities, but later, through a complex process of change, morphological diversification occurred. In addition to the basic name of ‘kimchi’, each variety has its unique name and history. This study was conducted through qualitative research using various research methods, such as oral records and interviews, as well as investigation of data from literature, including ancient literature, modern cookbooks, newspapers, magazines, papers, and videos. The study sought to investigate the context and the meaning of the name Chonggak kimchi. In addition, it is a compilation of how the name spread through the ages and evolved to its current name. The name Chonggak kimchi did not exist during the Joseon Dynasty and Japanese occupation and first appeared in the records in the late 1950s. Nevertheless, the original name of ‘Altarimu kimchi’ evolved and finally became a part of the standard Korean language (標準語) in 1988. In the process of the name spreading and becoming popular, the movie “Chonggak kimchi (1964),” starring Shin, S.I., and Eom, A.R. played a significant role. It was also confirmed that this was a meaningful and valuable case of contentization of traditional food culture, regardless of the intention behind the same.

글루탐산은 식물의 생장과 발달에 중요한 역할을 하는 필수 아미노산의 전구체이며, 저온 보호 물질로 이어지는 생합성 경로를 자극하여 저온 피해를 줄이는 생물자극제 중 하나이 다. 본 연구에서는 저온 스트레스 조건에서 글루탐산 엽면 처 리가 배추에 미치는 영향을 평가하였다. 글루탐산 2가지 엽면 시비 농도(0 및 10mg·L-1)와 3가지 주/야간 온도 수준(11/－1 °C extremely low, E; 16/4°C moderately low, M; 21/9°C optimal, O)을 결합하여 6개의 처리가 수행되었다. 글루탐산 의 엽면 처리는 정식 후 10일에 1회 살포하고, 글루탐산 처리 직후 온도 처리는 최대 4일 동안 실시하였다. 처리 4일 후, ABA, PA, DPA 및 ABA-GE 함량은 M 처리에서 Glu 0 처리 보다 Glu 10 처리에서 함량이 더 높았다. Glucose 함량은 E 및 Glu 10 처리에서 가장 높은 반면(52.1mg·100g-1 dry weight), fructose 함량은 O 및 Glu 0 처리에서 함량이 가장 높았다 (134.6mg·100g-1 dry weight). GLP, GBS, 4MGBS, GNBS 및 GNS 함량은 E 및 Glu 10 처리에서 모든 처리 중 가장 높았 다(0.72, 2.05, 1.67, 9.40 및 0.85μmol·g-1 dry weight). 처리 2일 후 E 및 Glu 10 처리의 PA와 DPA함량에서 급격한 변화 를 확인하였고, 몇몇 개별 glucosinolate 함량(GLP, GBS, 4MGBS, GNBS 및 GNS)은 저온과 글루탐산 처리에 따라 유 의적 차이를 확인할 수 있었다. 또한, fructose는 glucose 대신 fructan을 에너지원으로 사용하였기 때문에 처리 4일후 E와 M처리에서 O 처리에 비하여 유의적으로 낮은 함량을 보였다. 따라서, 저온과 글루탐산 엽면 처리에 따른 PA, DPA, glucose, fructose 및 개별 glucosinolate 물질들의 변이를 확 인하였지만, 저온과 글루탐산의 효과에 관한 명확한 상관관계를 평가할 수는 없었다. 배추과 작물은 호냉성 채소로서 저 온에 민감하게 반응하지 않고, 대부분 내한성을 가지고 있기 때문으로 판단된다.

Comparing the quality characteristics of kimchi were measured and anticancer effects using AGS human gastric cancer cells were observed. Five kinds of kimchi samples were made of Kanghwa Baek kimchi (KB), Kangwha Turnip kimchi (KT), Turnip: Chinese cabbage = 1:1 Baek kimchi (T1B1), Turnip:Chinese cabbage = 4:1 Baek kimchi (T4B1), Turnip mul kimchi (T). As a result T kimchi showed the best fermentation characteristics among the five samples. T kimchi had a lower percentage of the total number of aerobic bacteria, while the number of lactobacillus was higher than that of other samples. The mRNA and protein expression levels of apoptosis-related factors found that T kimchi significantly increases the mRNA expression levels of caspases-3 and caspases-9 in AGS human gastric cancer cells as compared to the other kimchi samples. It showed high anticancer effects in the order of T, T1B1, and KB kimchi. As the anticancer effect of Turnip mul kimchi made only of turnip was higher, the higher the turnip content, the higher the anticancer effect. These results show that there were changes in fermentation characteristics such as pH, acidity, number of lactic acid bacteria, and anticancer effects according to the ratio of turnip and cabbage.

Listeria monocytogenes (L. monocytogenes) is one of gram-positive foodborne pathogens with a very high fatality rate. Unlike most foodborne pathogens, L. monocytogenes is capable of growing at low temperatures, such as in refrigerated foods. Thus, various physical and chemical prevention methods are used in the manufacturing, processing and distribution of food. However, there are limitations to the methods such as possible changes to the food quality and the consumer awareness of synthetic preservatives. Thus, the aim of this study was to evaluate the anti-listeria activity of lactic acid bacteria (LAB) isolated from kimchi and characterize the bacteriocin produced by Lactococcus lactis which is one of isolated strains from kimchi. The analysis on the anti-listeria activity of a total of 36 species (Lactobacillus, Weissella, Lactobacillus, and Lactococcus) isolated from kimchi by the agar overlay method revealed that L. lactis NJ 1-10 and NJ 1-16 had the highest anti-listeria activity. For quantitatively analysis on the anti-listeria activity, NJ 1-10 and NJ 1-16 were co-cultured with L. monocytogenes in Brain Heat Infusion (BHI) broth, respectively. As a result, L. monocytogenes was reduced by 3.0 log CFU/mL in 20 h, lowering the number of bacteria to below the detection limit. Both LAB strains showed anti-listeria activity against 24 serotypes of L. monocytogenes, although the sizes of clear zone was slightly different. No clear zone was observed when the supernatants of both LAB cultures were treated with proteinase- K, indicating that their anti-listerial activities might be due to the production of bacteriocins. Heat stability of the partially purified bacteriocins of NJ 1-10 and NJ 1-16 was relatively stable at 60oC and 80oC. Yet, their anti-listeria activities were completely lost by 60 min of treatment at 100oC and 15 min of treatment at 121oC. The analysis on the pH stability showed that their anti-listeria activities were the most stable at pH 4.01, and decreased with the increasing pH value, yet, was not completely lost. Partially purified bacteriocins showed relatively stable anti-listeria activities in acetone, ethanol, and methanol, but their activities were reduced after chloroform treatment, yet was not completely lost. Conclusively, this study revealed that the bacteriocins produced by NJ 1-10 and NJ 1-16 effectively reduced L. monocytogenes, and that they were relatively stable against heat, pH, and organic solvents, therefore implying their potential as a natural antibacterial substance for controlling L. monocytogenes in food.

김치로부터 내산성, 내담즙성 및 항산화활성이 우수한 유산균을 분 리하였으며, 프로바이오틱스 활성이 우수한 2종의 균주를 스타터 로 선정하여 고농도의 홍삼 추출물이 보강된 요구르트를 제조하고, 그 품질특성을 평가하였다. 가정에서 제조된 배추김치로부터 분리 한 L. plantarum SY46과 L. brevis SY65 균주는 인공위액과 담 즙염에 대한 저항성, 병원균에 대한 항균활성 및 항산화활성이 상 업용 프로바이오틱스 균주인 L. rhamnosus GG(LGG)와 비교하 여 우수하였으며, auto-aggregation과 co-aggregation 능력은 유사한 특성을 나타내었다. 홍삼 추출분말 5%와 10%가 각각 첨가 된 우유에 SY46과 SY65의 복합 균주를 접종하고 24시간 발효한 결과, 홍삼 요구르트의 pH는 각각 4.46과 4.28, 산도는 1.25%와1.53%, 유산균 생균수는 9.26와 9.90 Log CFU/mL를 나타내었다. 홍삼의 첨가량이 증가할수록 DPPH 라디칼 소거능과 β-carotene bleaching 억제능이 증가되어, 유산균체의 항산화능과 더불어 홍 삼이 요구르트의 항산화능을 높이는데 기여하는 것으로 나타났다. 따라서 본 연구결과, L. plantarum SY46과 L. brevis SY65 균주 를 스타터로 활용하여 프로바이오틱스 및 홍삼의 기능성이 강화된 요구르트의 제조가 가능할 것으로 기대된다.

본 연구의 목적은 L. lactis 와 Leu. citreum 균주를 혼합 배양할 수 있는 배지 조성을 개발하여 이를 통해 김치의 발효 속도 조절을 통한 숙성도 맞춤형 김치를 제조하는 것이다. 먼저 김치 육수의 재 료인 북어, 양파, 무, 다시마, 건고추, 건새우, 대파, 건표고 등을 혼합 또는 단일로 하여 육수를 제조한 후, 이들이 L. lactis 증식에 미치는 영향을 알아본 결과, 건새우를 제외한 모든 재료가 증식을 억제하는 결과를 나타내었다. 질소원, 탄소원 및 성장 촉진 인자로 서 tryptone, peptone, yeast extract, lactose, ascorbic acid 등을 이용하였고 pH 완충제로는 potassium citrate를 사용하였 다. L. lactis가 잘 생장하는 M17배지와 균 생장 및 항균활성을 비 교 평가하여 최종적으로 배지 조성은 건새우 육수에 yeast extract 3%, ascorbic acid 0.05%, potassium citrate 1%, lactose 3% 로 하였다(식용 액체배지1). 개발된 식용 액체배지 1에 L. lactis 에 의해 생장 및 증식에 저해를 받지 않고 만니톨 생성능이 우수한 Leu. citreum을 혼합 배양하고자 glucose, maltose, fructose, sucrose 등 여러 가지 탄소원을 달리하여 Leu. citreum의 생육정 도를 살펴본 결과, 0.1-1%의 농도로 maltose 또는 fructose를 첨 가했을 때 선택적으로 잘 증식하였다. 이어서 발효 조절을 통한 숙 성도 맞춤형 김치를 제조하기 위하여 식용배지 1에다 maltose를 0%, 0.1%, 0.2%, 0.3%의 농도로 달리 첨가한 식용배지 2를 제조 하였다. 여기에 L. lactis 와 Leu. citreum을 혼합 배양한 후 김치 에 첨가하여 10℃에서 5일간 발효시킨 결과, 각 김치별로 pH 5.3, pH 4.9, pH 4.7, pH 4.5를 나타내어 각기 숙성도가 다른 특징을 보여주었다. 결론적으로 본 연구에서 개발한 식용 액체 배지를 사 용하여 L. lactis를 배양한 후 그 배양액을 김치에 첨가함으로써 종 균의 활성 및 생장을 극대화시킬 수 있었다. 또한 액체 배지 조성의 조절을 통해 Leu. citreum 균주와의 혼합 배양이 가능하였고, 이를 이용하여 김치 발효 속도 조절을 통한 숙성도 맞춤형 김치를 제조 할 수 있었다.

The salinity of the brining solution for preparing kimchi imparts saltiness to kimchi, and affects its overall taste. In this study, the changes in the overall taste and quality of kimchi depending on the salinity of the brining solution (6% and 12%) were investigated. The salinity of kimchi brined in 6% NaCl solution was approximately 1.3%, and its umami (2.22±0.22) was lower than that (2.96±0.16) of the conventional kimchi, brined in 12% NaCl solution. In contrast, its bitterness (-3.77±0.05) was higher than that of the conventional kimchi (-4.48±0.12). The sensory evaluation results showed that the overall taste score of the low-salinity kimchi (2.8±0.4) was significantly lower than that of the conventional kimchi (4.5±1.1). To overcome the deterioration in the overall taste, a taste enhancer with a sea tangle extract and anchovy extract was added to the low-salinity kimchi. The overall taste score (4.4±1.2) of the seasoned kimchi, which is the low-salinity kimchi supplemented with the taste enhancer, was comparable to that (4.5±1.1) of the conventional kimchi, while maintaining a salinity of 1.7%. This study demonstrated the benefits of proper taste enhancer for improving the taste of low-salinity kimchi.

GABA는 식물, 동물 및 미생물 등 자연계에 다양하게 존재하고 있으며, 항고혈압, 이뇨, 진정, 수면유도 및 항스트레스 등 다양한 효과가 있는 것으로 알려져 있어서 식품, 의약품 소재로 활용되고 있다. 본 연구에서는 김치로부터 분리한 GABA 형성 유산균을 옥수수 수염 추출물에 적용하여 발효 특성과 발효물의 항산화 효과에 대해 분석하였다. 신규 L. plantarum LAB459의 분리와 동정은 생화학적 특성, 당자화성 및 16s rRNA 염기 서열 분석을 통해 확인되었다. 그리고 TLC와 HPLC 분석을 통해 분리된 L. plantarum LAB459가 GABA 생성능이 있는 것으로 나타났다. 그리고 탈지유가 포함된 옥수수 수염 열수 추출물에 대해 발효를 수행한 결과, 동결 건조된 발효물에 약 1 μg/mg 수준의 GABA가 형성된 것을 확인하였다. 또한 발효물에 대한 플라보노이드와 항산화 분석은 옥수수 수염 열수 추출물에 비해 더 높은 것으로 나타났다. 따라서 본 연구에서 분리된 L. plantarum LAB459는 다양한 식품 발효의 스타터 또는 식품 소재와 의약품 소재로 적용이 가능할 것으로 판단된다.

본 연구는 김치에 사용되는 젓갈을 대체할 수 있을 것으로 예상되는 천연 소재들(고기추출물, 해산물추출물, 채소추출물)이 김치의 휘발성 향기 특성에 미치는 영향을 조사하였다. 모든 실험군에서 김치가 발효·숙성되면서 원재료에서 유래되는 황화합물이나 aldehyde류는 감소하고, alcohol류와 acid류 등과 같은 발효산물이 증가하여 김치의 휘발성 향미를 이루었다. 발효·숙성 28일째에 젓갈을 첨가한 김치에서 휘발성 물질 중 acetic acid가 가장 낮은 비율(4.90%)로 검출되었으며 채소추출물(5.39%), 대조구 (6.28%), 해산물추출물(6.47%), 고기추출물(8.08%)을 첨가한 김치 순서로 acetic acid가 높은 비율을 나타내었다. Acetic acid 비율이 증가함에 따라 산도가 증가하고 pH는 감소하였는데, 젓갈을 첨가한 김치는 숙성 28일에 가장 낮은 산도와 가장 높은 pH(산도 0.80, pH 4.37)를 나타내었으며, 그 다음으로는 채소추출물을 첨가한 김치(산도 0.84, pH 4.32), 고기추출물을 첨가한 김치(산도 0.89, pH 4.31), 대조구(산도 0.93, pH 4.27), 해산물 추출물을 첨가한 김치(산도 0.97, pH 4.23) 순서였다. 산도와 pH의 변화 그리고 발효산물인 acetic acid나 alcohol의 함량 비율로 볼 때, 김치에 첨가되는 젓갈 및 젓갈을 대체한 부재료는 발효·숙성에 시간적인 차이를 제공하는 것으로 생각된다. 담금 직후 (발효·숙성 전)에는 각각의 다양한 부재료로부터 유래되는 향미물질이 관능적 특성에 영향을 줄 수도 있으나, 김치의 휘발성 성분의 구성은 숙성이 진행됨에 따라 유사한 패턴으로 변화하는 양상을 나타내며 부재료에 대한 관능적 영향이 줄어들었다. 본 연구결과에 따르면, 젓갈과 젓갈을 대체하기 위한 천연 소재들은 초기 김치 담금시에 관능적 특 성에 영향을 주고 발효·숙성이 진행되는 속도에 영향을 줄 뿐, 최종 김치의 기호도에 영향을 주는 향기 특성과 관능적 특성에는 큰 영향을 미치지 않는 것으로 판단된다.

What is the origin of kimchi and what changes did it go through to become the kimchi of today? Also, what makes kimchi different from other pickled vegetables such as Chinese paochai, Japanese tsukemono, and Western pickles, and sauerkraut, and what is the identity of kimchi? This study is the result of thought on these fundamental questions about kimchi which is the only pickled vegetable distributed around the world that uses a fermented and ripened animal seasoning called jeotgal (salted marine life) to dramatically improve its umami taste and nutritional value, thereby securing its uniqueness. Kimchi has further evolved into a unique and high-quality pickled vegetable through the use of its specialized seasoning, adopting a composite fermentation mechanism and absorbing new ingredients such as red pepper. It is expected that this paper will invigorate the discussion on the firm establishment of the identity of kimchi and the future direction that it may take.

The Chinese Sigyeong records the foods of the Primitive Pickling Period, pickling being a universal vegetable storage method, but does not indicate the origin of the pickled vegetables or the location of the source of transmission. Kimchi mainly used salt and sauce-based soaking materials at the beginning of the Fermented Pickling Period (beginning in the 1st to 3rd centuries A.D.), and it differed from the Chinese method, which used alcohol and vinegar. In the Umami-Flavored Pickling Period (beginning in the 14th and 15th centuries A.D.), jeotgal, fermented seafoods, were added, and pickles with a completely new identity were created, one different from any other pickles in the world. Lastly, entering the Complex Fermentation and Pickling Period (beginning in the 17th and 18th centuries), the technical process evolved using a separate special seasoning containing red pepper as the secondary immersion source after pickling in brine, the primary immersion source. As a result of this, kimchi was transformed into a food with a unique form and taste not found anywhere else. The unique characteristic of kimchi is that the composition of original materials, a combination of salted marine life and vegetable ingredients, is its core identity, and there is a methodological difference in that it is completed through a second process called saesaengchae (生菜)-chimchae (沈菜).