Salmonella spp. and Brucella spp. have caused a considerable disease of farmed animals and economic loss in animal farming and food industry. In this study, the disinfection efficacy of Vital-Oxidel®, a commercial disinfectant, composed to chlorine dioxide, betaine hydrochloride, and propylene glycol was evaluated against S. typhimurium and Brucella ovis. A bactericidal efficacy test by broth dilution method was used to determine the lowest effective dilution of the disinfectant following exposure to test bacteria for 30 min at 4oC. Vital-Oixdel® and test bacteria were diluted with distilled water (DW), hard water (HW) or organic matter suspension (OM) according to treatment condition. On OM condition, the bactericidal activity of Vital-Oixdel® against S. typhimurium and Brucella ovis was lowered compared to that on HW condition. As Vital-Oxidel® possesses bactericidal efficacy against animal pathogenic bacteria such as S. typhimurium and Brucella ovis, this disinfectant solution can be used to control the spread of bacterial diseases.

Highly pathogenic avian influenza virus (HPAIV) is already panzootic in poultry and caused a considerable economic loss in poultry industry. In addition, HPAIV continues to cross species barriers to infect humans and other mammals, often with fatal outcomes. In this study, the virucidal efficacy of Citra-Kill® composed to quaternary ammonium chloride and citric acid was investigated against avian influenza H9N2 virus (AIV). A virucidal efficacy was determined with the viability of AIV contacted with the disinfectant in the allantoic membrane of chicken embryos. Citra-Kill® and AIV was reacted on the distilled water (DW), hard water (HW) or organic matter suspension (OM) condition. On DW condition, AIV was inactivated with 2,000 fold dilutions of Citra-Kill®. When the antiviral effect on HW condition was evaluated, the antiviral activity of the disinfectant showed on 1,500 fold dilutions against AIV. With the investigation of the antiviral effect of the disinfectant on OM condition, AIV was inactivated on 500 fold dilutions of Citra-Kill®. As Citra-Kill® possesses virucidal efficacy against AIV, the disinfectant solution can be used to limit the spread of animal viral diseases.

벼잎선충(線蟲)에 대(對)한 종자소독(種子消毒) 효과 및 본답(本畓)에서의 약제방제(藥劑防除) 체계(體系)를 구명(究明)코자 시험(試驗)한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 벼잎선충(線蟲)에 대(對)한 종자소독용(種子消毒用) 살충제(殺蟲劑)로 메프유제(乳劑), 다수진유제(乳劑), 펜치온유제(乳劑)의 약효(藥效)가 양호(良好)하였다. 2. 기존(旣存) 종자소독용(種子消毒用) 살균제(殺菌劑)인 베노람수화제(水和劑), 티시엠유제(乳劑), Proaz유제(乳劑)와 살충제(殺蟲劑), 메프유제(乳劑), 펜치온유제(乳劑), 다수진유제(乳劑)를 각각(各各) 혼용(混用)하여 종자소독(種子消毒)을 하였을때에도 약효(藥效)가 우수(優秀)하였으며 약해(藥害)도 없었다. 3. 본답(本畓)에서의 벼잎선충(線蟲)에 대(對)한 방제체계(防除體系)로는 종자소독(種子消毒)+카보입제(粒劑) 토중처리(土中處理)와 종자소독(種子消毒)+카보입제(粒劑) 6월하순(月下旬) 수면처리(水面處理)가 효과적(效果的)이었다.

비유기수은 종자소독제의 개발을 목적으로 배도열병균, 벼깨씨무늬병균 및 벼키다리병균에 감염된 증자 4개품종을 공시하고 Blotter 법, 물한천평판법 및 유묘 검정법 등을 통해서 12종의 약제에 대한 약효를 검정하는 동시에 검정방법별 차이를 검토하고 약해발현 여부등을 조사하였으며, 그 결과는 다음과 같다. 벼도열병, 벼깨씨무늬병 및 벼키다리병에 대하여 종합적으로 약효가 좋았던 것은 Sisthane, 및 Sodium Omadine이었다. Benlate T 및 Homai는 깨씨무늬병에 대한 약효가 낮았으며, Busan 30 및 Panoctine은 유묘검정에서 키다리병에 대한 약효가 낮았다. 약효검정방법으로서는 도열병에 대해서는 Blotter법이, 깨씨무늬병과 키다리병에 대해서는 물한천평판법이 좋았다. 발아율 및 초기생육에 미치는 약제의 최아전종자소독 결과, 약해의 유발은 없었다.

his study was carried out to investigate antimicrobial activities of water extracted Pleurotus ostreatus var Heuktari (PO), and their application to F. fujikuroi Nirenberg growth inhibition material. Various organic solvents (chloroform, hexane, ethyl acetate, ethanol) were tested to investigate the antimicrobial activities of Pleurotus ostreatus var Heuktari against F. fujikuroi Nirenberg. Chloroform, hexane, ethyl acetate and hot water extracts had no antimicrobial activities, but butanol extract showed 2.5% strong activities in order of F. fujikuroi Nirenberg disk diffusion test. Then we observe Antifungal activity using green house. Bed soil and mushroom extract mixed at 10%, 7.5%, 5%, 2.5%, 1% total bed soil mass. Screening of Antifungal activity was tested two periode 18day and 25day. The Antifungal activity rate of each period and extract density was 7.5% mixed bedsoil has investigated 80% Antifungal activity rate. The result indicates that the Pleurotus ostreatus var Heuktari extract using F. fujikuroi Nirenberg registance, which shows the development of organic agriculture seed disinfectant.

Food waste, food leachate and livestock wastes from an usual farm and a farm using much disinfectant were mixed to incubate within anaerobic serum bottle for BMP test. The methane yield rate and lag phase were determined by the modified Gompertz model and the Logistic model. The maximum methane yield rates by the modified Gompertz model were 15.9 ~ 41.0 mL CH4/g VS and higher than by the Logistic model. The modified Gompertz model was more appropriate than the Logistic model to have higher determination coefficient R2. The methane fermentation of mix with sole livestock waste from the farm using much disinfectant had ninefold lag phase and 40% or lower maximum yield rate comparing with the mix with sole usual livestock waste. The methane yield rate from a tonne of mix was increased as the ratio of food waste and food leachate increased. The cumulative methane yield was in the proportion of 40 m3 to a tonne of food wastes. The results of BMP test were analyzed by a response surface methodology (RSM) and modelized to a binomial expression, which was verified by analysis of variance (ANOVA) and to be appropriate for this case.

This study was conducted to evaluate the effect of seed disinfectant, in control of brown leaf blight, growth characteristics, and dry root yield in the cultivation of Alisma plantago after early maturing rice cropping. Experimental plot was laid out in split plots design with three replications. The major seed disinfectants were benomyl Wp, 20%, Captan Wp, 50%, Triferine Ec, 17%, Etridia zole Ec, 25%, and Thioplant-mythyl Wp, 50%. Even though seed disinfectant treated had no effect on the growth and flowering date of Alisma plantago, dry root yield was increased largely with benomyl Wp, 20%, in seed disinfectant than in the other seed disinfectants and contorl. All seed disinfectants had no injury with standard dosage. But all seed disinfectants had slight injury in the double dosage level for the Alisma plantago. On the basis of yield, vegetative and disease paramerer, benomyl Wp (20%) (100g/20l) had shown superior performance, however, all the seed disinfectants are effective as compare to without treatment.

The antibacterial effect amino acid-copper(Ⅱ) surfactant on fish pathogens was studied. Fish pathogens of Edwardsiella tarda, Vibrio anguillarum, Aeromonas hydrophila and Streptococcus sp. were selected, cultured in nutrient agar and adjusted at 2 × 10 5∼106 CFU/㎖ in phosphate buffer saline before the addtion of amino acid-copper(Ⅱ) surfactant with different concentrations. All tested pathogens died within 1 hour with 1 ppm of amino acid-copper(Ⅱ) surfactant. In comparison with formalin and ET, amino acid-copper(Ⅱ) surfactnat was more effective in antibacterial capacity.

본 연구는 율무 종자소독제인 Fludioxonail 10% 종자처리 액상수화제의 침종기간이 발아율 및 병발생에 미치는 효과를 구명하기 위해 수행된 결과를 요약하면 아래와 같다. 시내 실험에 있어 율무종자를 3일간 종자소독 후 3일 침종처리시 발아율이 93.8%로 기타 처리구에 비해 높았으며, 부패율은 침종기간이 길수록 높아지는 경향이었다. 폿트 및 포장시험에서 율무종자를 3일간 종자소독 후 3일 침종처리시 출현율이 기타 처리구에 비해 높았으며, 입고병은 침종기간이 길수록 심해지는 경향 이었다. 포장재배에서 생육상황은 약제처리간 큰 차이가 없었으며, 10a당 조곡 수량은 무처리 194kg에 비해 3일간 종자소독 후 3일 침종처리구와 6일 종자소독구에서 26%~27% 증수하였다. 이상의 실험결과를 종합하여 볼 때 율무의 출현율향상 및 병발생을 줄이기 위해서는 3일간 종자소독 후 3알 침종처리 또는 6일 종자소독이 적당하다고 여겨진다.

본 연구는 율무 주산단지인 연천지역에서 율무 종자전염병인 잎마름병 및 깜부기병에 대한 종자 소독 실태와 종자소독제 처리에 의한 방제효과를 검정한 결과를 요약하면 아래와 같다. 율무의 잎마름병 종자감염율은 연천지역 농가에서 수집한 종자를 검사한 결과 32.7~45.7%였으며, 율무종자 소독기간은 율무 1호가 0~7일, 연천 재래종이 0~20일이었다. 농가별 종자소독기간 분포 비율은 무소독 20%, 1~5일 20%, 6~10일 55% 그리고 11일 이상이 15%였다. 종자소독처리제인 후루디옥소닐종자처리액상수화제의 잎마름병에 대한 종자소독효과는 97%, 깜부기병에 대한 종자 소독효과는 93.4% 로 높은 방제가를 나타냈다.