This study examines the effects of different cooking methods (stir-frying, steaming, superheated-steaming) on the contents and the true retention of moisture, water-soluble vitamins, and bioactive compounds of ten selected vegetables: broccoli, brussels sprout, cabbage, eggplant, green bean, onion, red cabbage, red onion, squash, and tomato. The total color difference (ΔE) values were decreased after stir-frying the samples, except for eggplant, green bean, and tomato. The true retention of water-soluble vitamins (B1, B2, B3, and C) was increased in most vegetables after superheated-steaming, as compared to steaming and stir-frying. Moreover, compared to the uncooked vegetables, a higher true retention of total polyphenol and flavonoid was obtained for most vegetables subsequent to superheated-steaming. Total anthocyanin content was detected only in eggplant, red cabbage, and red onion, and a smaller loss of anthocyanin was determined after subjecting red cabbage to superheated-steaming. Also, the free radical scavenging activities were higher in superheatedsteaming vegetables, except in eggplant and squash. These results indicate that superheated-steaming induces a positive effect for retaining water-soluble vitamins and functional components of vegetables.

The purpose of this study was to determine the contents of water-soluble vitamins B1 (thiamine), B2 (riboflavin), B3 (niacin) and B12 (cyanocobalamin) in seafood-based dishes by using the high-performance liquid chromatography with the ultra violet and fluorescence detector. The vitamin B1, B2, B3 and B12 contents were analyzed in 29 seafood-based dishes. The method validation was performed on the method to determine the linearity, accuracy, limits of quantification, limits of detection and precision for vitamin B1, B2, B3 and B12. An excellent linearity range (R2=0.999~1.000) in the calibration curve for the water-soluble vitamins was observed. All analytical methods for the water-soluble vitamins showed the acceptable accuracy (89.4~119.7% recovery) and the precision (0.6~4.8% repeatability and 1.0~4.2% reproducibility). The highest content of vitamin B1 was 2.646 mg/100 g in the kkongchi-jorim, and the highest contents of vitamin B2, B3 and B12 were 0.370 mg/100 g, 10.971 mg/100 g, and 17.193 μg/100 g, respectively, in the kkongchi-gui. Our results provide reliable data on the contents of the water-soluble vitamins of seafood-based dishes in Korea.

건강기능식품 등 중 수용성 비타민 B군 (비타민 B1, 비타민 B2, 나이아신, 비타민 B6)의 동시분석 방법을 확립하기 위하여 본 연구를 실시하였다. HPLC에 의한 수용성 비타민 B군의 분석 방법은 기기분석조건을 확립하고, 국내외 시험법 및 논문을 참고하여 시험 데이터를 분석하였다. 수용성 비타민 B군의 HPLC 분석은 270 nm에서 Capcell Pak C18 UG 120 컬럼을 사용하여 진행되었다. 분석법 검증은 수용성 비타민 B군의 직선성, 정확성, 정량한계, 정밀성에 대해 수행되었다. 직선성은 비타민 B1, 비타민 B2,나이아신(nicotinic acid, nicotinamide), 비타민 B6 모두0.1~2 μm/mL의 농도범위에서 R2= 0.999 이상의 우수한 직선성을 확인할 수 있었다. 회수율을 검토한 결과, 비타민 B1 100~103%, 비타민 B2 104~112%, nicotinic acid 82~85%, nicotinamide 121~124%, 비타민 B6 95~104%의 양호한 회수율을 확인할 수 있었다. LOQ는 비타민 B1 0.04μm/mL, 비타민 B2 0.05 μm/mL, nicotinic acid 0.15 μm/ mL, nicotinamide 0.08 μm/mL, 비타민 B6 0.63 μm/mL 이었다. 정밀성을 검토한 결과, B1 0.4%, 비타민 B2 0.4%, nicotinic acid 0.5%, nicotinamide 0.7%, 비타민 B6 0.4% 로 1% 이내로 양호한 재현성을 확인할 수 있었다. 또한 동시 분석법의 정확성을 검증하기 위하여 어린이 기호식품 및 건강기능식품의 표시함량 모니터링을 수행한 결과, 모두 표시기준에 적합함을 확인할 수 있었다.

This study was done to analyze the contents of minerals and vitamins to compare the measured values of minerals, vitamins with labeled values of them in food labeling and to investigate the ratio of measured values to labeled values in 437 specimen with minerals and vitamins - fortified commercial beverages and liquid teas. Content of calcium and sodium in samples after microwave digestion was analyzed with an ICP-OES (Inductively Coupled Plasma Optical Emission Spectrometer) and vitamins were determined using by HPLC (High Performance Liquid Chromatography). The measured values of calcium were ranged 80.3~142.6% of the labeled values in 21 samples composed calcium - fortified commercial beverages and liquid teas. In case of sodium, measured values were investigated 33.9~48.5% of the labeled values in 21 sports beverages. The measured values of vitamin C, vitamin B2and niacin were ranged 99.7~2003.6, 81.1~336.7, 90.7~393.2% of the labeled values in vitamins - fortified commercial beverages and liquid teas, 57, 12, 11 samples. To support achievement of the accurate nutrition label, there must be program and initiatives for better understanding and guidances on food labelling and nutrition for food manufacture.

In this study, synergic effects of MEM vitamins (MEMv) and beta-mercaptoethanol (bME) supplemented to porcine maturation medium on reactive oxygen species (ROS) of oocytes and embryos, and apoptosis of blastocysts were determined. Cumulus-oocyte- complexes (COCs) were allocated into four treatment groups: 0.05X MEMv, 25 uM bME, 0.05X MEMv + 25 uM bME or a group without MEMv + bME. In experiment 1, COCs were cultured in four respective treatment groups based on NCSU-23 medium for 44 h at 39℃ in a 5% CO2 atmosphere. We evaluated ROS of oocytes. In experiment 2, COCs were cultured in four respective treatment groups and then were fertilized in vitro (IVF) or activated by chemical or electrical method. We determined ROS of early stage embryos (2 cell-4 cells) and apoptosis of blastocysts. DCHFDA for ROS level and TdT-mediated dUTP nick end labelling (TUNEL) for apoptosis were used. As a result, ROS level of oocytes was not significant difference among experimental groups. In early stage embryos produced by IVF, MEMv + bME group showed significantly lower ROS level than that of control group (p<0.05). Level of apoptosis in blastocysts of the MEMv + bME group was significantly lower than that of the control group (p<0.05). In early stage embryos produced by chemical activation, ROS level of MEMv + bME group was significantly lower than that of bME group (p<0.05) without significant difference with those of control and MEMv group. Level of apoptosis in blastocysts in the MEMv + bME group was significantly lower than that of the control group (p<0.05). In early stage embryos produced by electrical activation, ROS level of MEMv + bME group was significantly lower than that of control (p<0.05). However, apoptosis level of blastocyst was not significant difference among experimental groups. In conclusion, the present study indicates that the addition of MEM vitamins and betamercaptoethanol during in vitro maturation is able to alleviate the production of ROS and apoptosis.

This study was performed to investigate patterns of fortified food (FF) consumption and intake of vitamins and minerals from FFs among 577 Korean children (12.4 years of age) who attended elementary or middle school. FFs eaten by children as a snack were surveyed using the food record method during 3 days, including 2 week days and one weekend. As a result, 114 FF items were eaten by the children, and several kinds of nutrients such as vitamin A, D, E, B complex, C, calcium (Ca), iron (Fe), and zinc (Zn) were fortified in these foods. Ca-FFs (65.8%) were most frequently consumed, followed by vitamin C-FFs (33.4%) and vitamin D-FFs (33.3%). The number of FF items in each food group was the most in the milk group (n=24, 21.0%), followed by the beverage group (n=19, 16.7%), and the cookie/bread/cake group (n=17, 14.9%). Fortified nutrients in FFs were in various combinations, but the major combination patterns were Ca, Ca plus vitamins, Ca plus vitamins plus other minerals, and Ca plus other minerals. Daily mean intakes of vitamins and minerals from the FFs were 66-300% more than those of the recommended nutrient intake (RNI ) or adequate intake (AI) for most vitamins and minerals. Daily maximum intakes (95th percentile) of vitamins and minerals from FFs were 1-15 times the RNI or AI for most vitamins and minerals. Vitamin and mineral consumption ratios from each FF group were different according to the kind of fortified nutrient. For example, vitamin C was mostly eaten in fortified beverages (46-54%), and Fe was mostly eaten in fortified cookie/breads/cakes (87%). The above results show that FF consumption varied widely among the children, and that most of the children's foods were fortified with several vitamins and minerals without a common rule; thus, subjects risked over consuming vitamins and minerals by eating FFs. Therefore, practical guideline on FF use for children's optimal nutrition and health should be provided through nutrition education.

본 연구는 건강한 남자 대학생을 중심으로 흡연 여부에 따른 흡연에 대한 인식 및 항산화 영양소 섭취수준과 혈장 농도를 비교하고자 흡연자 30명, 비흡연자 30명을 선정하여 신체계측과 설문조사를 통해 다음과 같은 결과를 얻었다. 대상자의 연령은 흡연자가 24.4세 비흡연자가 22.4세이고, 신장은 각각 173.4cm, 172.6cm, 체중은 69.8kg, 67.9kg으로 나타났으며, BMI, 체지방, 체지방량, 제지방량(LBM), 체수분량(TBW)에서 군간 유의적인 차이가 없었다. 수축기 혈압 및 이완기 혈압은 흡연자에게서 유의적으로 높았으며, 혈당은 유의적이지는 않았지만, 흡연자가 높은 경향을 보였다. 커피와 알코올을 섭취하는 비율은 모두 흡연자에서 유의적으로 높았으며, 일일 섭취하는 알코올의 양도 흡연자에서 유의적으로 높았다. 흡연자들의 흡연 이유로는 '스트레스 해소'가 가장 많았고, 다음으로는 '습관적', '대인관계 향상을 위해서' 순이었다. 흡연여부에 따라 흡연에 대한 인식도를 조사한 결과, 흡연자가 비흡연자에 비해 유의적으로 흡연에 동의하는 경향이 높은 것으로 나타났다. 식품섭취 빈도조사에 의한 항산화 비타민 섭취량은 비타민 A의 경우 흡연자 505.15±123.32gR.E. 비흡연자 519.48±111.25μgR.E.로 유의적 차이는 없었다. 비타민 C는 각각 133.13 37.76mg, 124.68±27.12mg로 유의적이지는 않았지만 흡연자가 약간 높았으며, 비타민 E 섭취량은 6.91±2.39mg, 7.09±1.73mg로 차이를 보이지 않았다. 신체 계측치와 생화학적 성분간 상관관계에서는 이완기 혈압과 연령, 체지방, 체지방량이 양의 상관관계를 보였고, 혈장 비타민 C 농도는 체중(p＜0.05), 체수분량(p＜0.05)과 음의 상관관계가 있는 것으로 나타났다. 본 연구의 결과 혈압이나 혈당에서 흡연자가 비흡연자에 비해 높은 수준을 보여 흡연이 건강에 부정적인 영향을 미치는 것으로 나타났으며, 흡연에 대한 인식에서는 흡연자가 흡연에 동의하는 경향이 더 높았다. 흡연자와 비흡연자의 항산화 비타민 섭취량은 차이가 없었으나 활동량이 많고, 흡연력이 짧으며, 식이 섭취량이 비교적 많은 남자 대학생을 대상으로 하였음에도 불구하고 혈장의 비타민 C 농도가 유의적으로 낮았던 것은 흡연으로 인해 비타민 C 요구량이 증가된 것이라고 볼 수 있다. 따라서 항산화 영양소의 급원이 될 수 있는 식물의 섭취를 증가시키고 올바른 식습관을 형성하기 위한 교육이 이루어져야 할 것으로 사료된다.

Lipid peroxidation is one of the main manifestations of oxidative damage and has been found play an important role in the toxicity and carcinogenesis of many carcinogens. This study was carried out to investigate the effects of aflatoxin B₁ co-administrated with antioxidant vitamins on lipid contents and fatty acids components of liver in mice. For this work, vitamin C and vitamin E, the major antioxidants, were administrated with 10 ㎎/㎏ and 63.8 ㎎/㎏ respectively, through intraperitoneal(i.p) injection to male ICR mice, and 0.4 ㎎/㎏ of the AFB₁ injected by i.p. lhr later. The results were as follows: two fold amounts of free cholesterol, triglyceride, and total cholesterol in serum and liver of mice treated with only AFB₁ were observed, when compared to those of mice co-administrated with antioxidant vitamins. However, the levels of phospholipids in serum and liver of mice treated with only AFB₁ were decreased. Concerning to fatty acids composition of liver from AFB₁-treated mice, P/S ratio was shown more low level in cholesteryl ester, triglyceride, total cholesterol and phospholipid than those of mice co-administrated with antioxidant vitamins. In these data which provide with a reliable evidence on their antioxidantal effects to aflatoxicosis.

This study examines the effects of the Korean horticultural foods which are, by traditional wisdom, supposed to contain anti-aging substance. All twenty three kinds of plants are chosen as a sample for the experiment in which the content of three main anti-aging nutrients, -carotene, Vitamin C, and Vitamin E are respectively measured by Nilis, Colorimetric, and AOAC method. The result shows: among the samples, top six plants that contain the most -carotene content are high quality tea(Camellia sinensis), thistle(Cirsium japonicum Var.), Angelica keiskei, mulberry leaves(Morus alba l.), field horsetail(Equisetum arvensa), and carrot(Daucus carota), in order; top four for a Vitamin C contents are low quality tea, mulberry leaves, pine leaves(Pinus densiflora), and high quality tea, in order; top four for a Vitamin E contents are persimmon leaves(Diospyros mongolicum) tea, high quality tea, low quality tea, and pine leaves, in order. Drying or boiling process increases the content of -carotene and Vitamin E in high quality tea, dropwort(Oenanthe javanica), field horsetail, and wild aster(Ligularia stenocephala Marsumum et Koldzumi), while, in case of mulberry leaves, drying process lowers them. Vitamin C content strikingly decreases in heating and drying process.

This study was carried out to measure the contents of moisture, crude ash, crude fat, total amino acid, with amino acid composition, vitamin C, β-carotene, vitamin E, total catechins, EGCG, EGC, ECG, EC, GA, caffeine, theobromine and theophylline of the green tea I, II, III, oolong, and black tea. The content of crude fat of green tea I, II, III, oolong, and black teas was 1.1, 2.5, 4.9, 0.8 and 1.2% respectively, total amino acid content was 0.87, 0.78, 0.60, 0.63 and 1.05% respectively, and theanine content was 0.52, 0.48, 0.31, 0.41 and 0.61%, respectively. Total amino acid content of green tea increased in the order of green tea I $gt; green tea II $gt; green tea III, and among the teas, the content of theanine was the highest in the amino acids present. The content of vitamin C of green teal, II, III, oolong, and black tea was 101.6, 87.5, 95.9, 99.1 and 108.0 mg%, respectively, β-carotene content was 270, 268, 481, 80 and 181 ppm, respectively. Among the α-, β-, γ- and δ-tocopherol, the content of α-tocopherol was the highest in vitamin E present, and β- and δ-tocopherol were not detected in the samples of green teal, II, III, oolong, and black teas. The total catechins of green teal, II, III, oolong, and black teas was 10.5, 10.4, 7.2, 8.4 and 1.8% respectively, and among them, EGCG content was the highest. The content of EGC increased in the order of green tea I $gt; green tea III $gt; green tea II $gt; oolong tea $gt; black tea. The contents EGCG and ECG increased in the order of oolong tea $gt; green tea I $gt; green tea II $gt; green tea III $gt; black tea, and the highest contents of EGCG and ECG were observed in the samples of oolong tea. The content of GA was 0.01, 0.02, 0.05, 0.13 and 0.31%, respectively, and the highest contents of GA, caffeine and theobromine were observed in the sample of black tea. The highest content of theophylline, however, was observed in the sample of green tea I.

Influence of vitamins on the felt and lipid production of Aspergilus niger var. macrosporus, Aspergillus fumigatus and penicillium notatum were investigated after 10 days of incubation at 30℃ under static culture condition. The felt of molds were lower in the media containing vitamins than control medium. The lipids produced by each strains were highest yields in media containing vitamins. Among of these Aspergillus niger var. macrosporus and Aspergillus fumigatus produced. 4.6g and 5.28g in medium containing inositol 2.5mg/l and 0.5mg/l, respectively; while Penicillium notatum produced 1.51g in a medium containing thiamine 10mg/l. The major fatty acid of lipids were palmitic acid, stearic acid, oleic acid and linoleic acid. Aspergillus niger var. macrosporus was lower palmitic acid in media containing vitamins than control medium, While linoleic acid was higher in media containing thiamine and inositol. Aspergillus fumigatus was lower oleic acid in media containing vitamins than control medium, While linoleic acid was higher in media containing vitamins. Peniillium notatum was lower palmitic acid, oleic acid and stearic acid in media containing vitamins but linoleic acid was higher. The composition of fatty acid of lipids changed depending on the media containing vitamins but hardly found a certain tendency except linoleic acid which was higher in a media containing thiamines and inositols. The degree of unsaturation of fatty acids in the lipids were comparatively higher in media containing vitamins than control medium.

목련흰비단병균 Sclerotium rolfsii의 균사생장 및 균핵형성에 대한 thianine, biotin, nicotinic acid, Pyrido-xine, inositol과 DNA 및 RNA의 영향을 조사한 바 그 결과를 요약하면 다음과 같다. 1. 공시균은 thiamine 결핍균으로서 균사생장 최적 thiamine 농도는 20r/l이고 이농도를 초과하면 오히려 균사생장이 억제되어 150r/l에서는 무첨가구와 거이 비슷한 균사생장을 하였다. 2. 공시균의 생장에 있어서 thiamine의 첨가에 따른 질소원 이용도는 NH4NO3>(NH4)2SO4>asparagine>KNO3의 순위이며 질소원별 thiamine 최적요구량은 인 경우 12r/l, asparagine인 경우는 16r/l정도였다. 균핵형성량에 있어서는 KNO3>NH4NO3>asparagine>(NH4)2SO4의 순위로 질소원별 thiamine 최적량은 가 8r/l에서 균핵의 대부분이 형성되나 asparagine은 16r/l정도였다. 3. 배양액의 pH는 공시균이 생장을 개시하자마자 3.5정도로 급격히 떨어지나 그 이후로는 생장량이 증가함에 따라 완만하게 떨어졌다. 그러나 pH2.2 이하로는 내려가지 않았다. 4. Nicotinic acid는 공시균의 생장 및 균핵형성에 아무런 효과가 없었다. 그러나 thiamine 10r/l와의 공존하에서는 다소의 효과가 있는 것으로 nicotinic acid 7-10mg/l에서 가장 생장이 좋았고 그 이상에서는 생육이 억제되었다. 5. Pyridoxine, biotin 및 inositol은 배지의 질소원이 나 asparagine인 경우 모두 공시균의 균사생장 및 균핵형성에 아무런 효과가 없었다. 6. 공시균의 균사생 장에 대한 각종 vitamin의 상호효과는 thiamine, biotin, Pyridoxine, inositol의 4가지 combination에 의해서도 thiamine이 첨가되지 않으면 거이 효과가 나타나지 않았다. 그러나 thiamine+pyridoxine, thiamine + inositol, thiamine + biotin + pyrid oxine, thiamine + biotin + pyridoxine + inositol 구에 있어서는 thiamine단독첨가구와 동등 혹은 그 이상이지만 thiamine+biotin과 thianline+biftin+inositol구는 오히려 떨어졌다. 균핵형성에 있어서는 thiamine단독구에 비하여 각구 모두 약간씩 증가하였다. 7. 공시균의 균사생장에 대한 DNA와 RNA의 효과는 무첨가구에 비하여 다소 인정되는데 DNA보다는 RNA가 좀더 효과적이었다. RNA는 2-6mg/l에서 DNA는 6mg/l에서 가장 좋았다. 균핵형성에 있어서는 효과가 없 었다. 8. RNA의 농도가 증가함에 따라 또 thiamine의 농도가 증가함에 따라 균사생장량이 증가하는 것으로 thiamine의 존재하에 RNA의 효과가 뚜렷하게 나타났다. 그러나 균핵형성에는 효과가 없었다.

강원도 홍천 유기농 재배단지 내에서 재배한 5종의 유기농 야채 즉, 케일, 신선초, 셀러리, 상추 및 파와 가락동 시장에서 구입한 일반재배 야채를 시료로 하여 일반성분 함량, 무기물, 비타민 및 chlorophyll 함량을 각각 측정하여 유기농 야채 판별을 위한 기초자료로 활용하였다. 일반성분 함량은 전체적으로 유기농 야채의 조단백, 조지방 함량이 일반야채 보다 높아 이는 야채의 외관에도 직접적인 영향을 미치는 것으로 판단되며, 상대적으로 녹색색

To produce several high-quality species of Chwinamul (Aster scaber, A. Tataricus, Ligularia fischeri), leaves of the plants grown in different natural habitats were anlayzed. Contents in vitamin C and β-carotene were generally higher in plants grown in the field. However, Aster scaber picked form Yongwol and A. tataricus from Mt.Taryong contained relatively higher vitamin c and β carotene. Crude protein and fat contents varied depending on the growing area. Ca content was markedly higher in the plants grown in the natural havitats.