간행물
한국응용곤충학회 학술대회논문집
- 발행기관 한국응용곤충학회
- 자료유형 학술대회
- 간기 반년간
- 수록기간 2000 ~ 2024
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 495DDC 595
권호리스트/논문검색
2010 한국응용곤충학회 정기총회 및 춘계학술발표회 (2010년 5월) 225건
201.
2010.05
구독 인증기관·개인회원 무료
Five field-collected populations (TR-CP, UR-CP, JR-CP, GR-CP, and BR-CP colonies) of northern house mosquito, Culex pipiens pallens, identified by polymer chain reaction (PCR) were tested for susceptibility to 10 different insecticides by direct-contact mortality bioassay in comparison with an insecticide-susceptible KS-CP strain. Marked regional variations of insecticide susceptibility were observed. Extremely high to high levels of resistance were obtained: bifenthrin, resistance ratio (RR) = 1–520; β-cyfluthrin, RR = 16–397; α -cypermethrin, RR = 9–343; deltamethrin, RR = 1–40; etofenprox, RR = 2–42; permethrin, RR = 2–12; chlorpyrifos, RR = 2–675; fenitrothion, RR = 0.5–364; and fenthion, RR = 2–360. All of the colonies were susceptible to one or more of the insecticides examined. These results indicate that careful selection and rotational use of these insecticides should result in continued satisfactory control against field populations of northern house mosquito.
202.
2010.05
구독 인증기관·개인회원 무료
파리목 모기과에 속하는 모기는 인간에게 질병을 매개하는 위생해충으로 세대기간이 짧으며, 번식력이 강한 특성을 가지고 있다. 인간에게 피해를 주는 모기에 대한 국내의 모기 방제는 대부분 성충을 대상으로 분무식 화학농약을 처리하였으나성충의 휴식 및 행동습성 그리고 정확한 서식처에 직접 접촉을 해야 방제효과를 얻을 수 있다는 점에서 방제에 어려움이 있다. 본 연구에서는 피해의 본질적인 원인이 되는 모기 유충을 대상으로 곤충 살충성 단백질을 생성하는 Bacillus thuringiensis(이하Bt)균주를 선발하여 친환경적인 모기 방제에 대한 실험을 수행하였다. 국내의 토양에서 Spherical type의 Crystal을 형성하는 19개 균주를 위상차 현미경으로 확인 후 모기에 가장 높은 활성을 보인 B.thuringiensis subsp. israelensis CAB191균주를 선발 대량배양하였다., 생물검정은 현재 시판중인 B.t.i 약제(B제품) 와 에스메토프렌을 주성분으로 하는 천연 식물성 테르페노이드 화합물인 약제(A제품)를 대조구로 사용하였다. 대상 해충은 이집트숲모기(Aedes aegypti), 이나도미집모기 (Culex inatomii), 지하집모기(Culex pipiens molestus)의 3령 유충으로 생물 검정을 수행하였다. CAB191균주와 B제품은 103cfu/ml에서 이집트숲모기, 이나도미집모기, 지하집모기에 90%이상의 높은 사충률을 보였다. 반면 A제품은 1,000배 희석액에서 48시간 후 검정시 10%정도의 사충률을 보였다. 이로써 CAB191균주와 B제품이 활성이 높음을 알 수 있었다. 또한 B.t.i와 B제품, CAB191, 대조 균주로 B.t.morrisoni의 단백질 패턴을 분석하기 위하여 SDS-PAGE한 결과 CAB191, B제품이 B.t.i와 동일한 130, 72, 27KDa의 밴드를 형성함을 알 수 있었다.
203.
2010.05
구독 인증기관·개인회원 무료
해충에 대한 화학농약의 오남용으로 인하여 해충의 저항성이 발달되고 환경오염, 인축에 대한 위험 등의 이유로 화학농약의 사용을 줄이게 되었다. 그래서 친환경적인 방제인자인 미생물농약 Bacillus thuringiensis(이하 B.t)가 현재 전 세계적으로 널리 사용되고 있다. B.t는 그람 양성의 호기성 간균으로 토양, 곡물의 분진, 낙엽, 곤충의 사체등 다양한 곳에서 분리되는 것으로 알려져 있는데 본 연구에서는 풍뎅이류 곤충의 사체로부터 B.t를 분리하여 딱정벌레목 및 나비목 해충에 대한 생물활성 검정을 하여 살충활성이 우수한 균주를 선발하였으며 SDS-PAGE를 수행하였다. 파밤나방에 대한 생물활성 검정 결과, CAB530 균주는 처리 48시간 후에 90%가 넘는 사충률을 보여주었으나 CAB533 균주는 6%의 사충률을 보여주었다. 반면에 장수풍뎅이 유충과 배추벼룩잎벌레 성충에 대해서는 살충활성을 나타내지 않았다. SDS-PAGE 수행 결과 파밤나방에 살충활성이 있는 CAB530 균주는 B.t subsp. kurstaki와 비슷한 130kDa의 밴드를 나타냈으며 파밤나방 중장액으로 소화를 시킨 후에 약 65kDa의 밴드를 확인할 수 있었다. 파밤나방에 대해 살충활성이 적은 CAB533 균주는 65kDa의 독소단백질 밴드를 나타냈지만 파밤나방에 대해서 살충활성이 높지는 않았다.
204.
2010.05
구독 인증기관·개인회원 무료
In agricultural fields, the entomopathogenic fungal species have been investigated for their potential as the biological control agents due to their role of natural enemies for insects. To address the requirements of a potential South Korea based biocontrol effort using entomopathogenic fungi, we investigated the occurrence of various entomopathogenic fungi in 1080 soil samples representing from various area and locations in South Korea. Entomopathogenic fungi were isolated from soils using semiselective medium SDA-D50 contained saboraund dextrose agar, 50 ug/ml dodine, 100 ug/ml chloramphenicol and 50 ug/ml streptomycin. The isolated putative fungi were identified by the determination of internal transcribed spacer (ITS) region sequences of the nuclear ribosomal analysis. As a result, entomopathogenic fungi were found to occur in 30.8% of the soil samples studied. The most abundant species were Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metschn.) Sorok. Isolates of B. brongniartii, Cordyceps sp., Lecanicillium sp., Isaria sp. and Tolypocladium cylindrosporum were also found. The occurrence of entomopathogenic fungi was analyzed by the area and soil types. These positive entomopathogenic fungi may have potential against variety pests in agriculture and forest
205.
2010.05
구독 인증기관·개인회원 무료
Twospotted spider mite, Tetranychus urticae was collected from the rose greenhouse in Gimhae, Gyeongnam province in Jan, 2001. Resistant strain was reared over nine years treated with Acequinocyl (over 400 times), and increased 614 folds as compared to susceptible strain (S). Cross resistance of acequinocylresistant strain adults to eight acaricide was showed high in Cyflumetofen (108 folds) and expressed low in Abamectin (16 folds), Tebufenpyrad (7.6 folds), Fenpyroximate (6.1 folds). Against Chlorfenapyr (0.5 folds), however, showed negatively correlated cross resistance. Synergistic effects with PBO (piperonyl butoxide), an oxygenase inhibitor, DEF (S,S,S-tributyl phosphorotrithioate), an esterase inhibitor, and DEM (diethymaleate), an inhibitor of glutathion S-transferase were also investigated. Synergistic effect showed low in DEM (4.2 folds) but showed high in PBO (12 folds) and DEF (23 folds).
206.
2010.05
구독 인증기관·개인회원 무료
This study was performed to investigate attraction effect of six plant essential oils and to confirm their electrophysiological response against Lycorma delicatula. Among the tested oils, spearmint oil (94.1%) significantly attracted L. delicatula at a dose of 1.25㎕/cm2 by using an olfactometer. In dose response to spearmint oil, a dose of 2.5㎕/cm2 was very effective. GC-MS analysis revealed that the active components responsible for the effective attraction effect of spearmint oil were carvone (70.6%) and limonene (54.8%). Of the two active components, carvone was more significant than limonene with reference to attraction activity against L. delicatula. Analysis by GC-EAD showed, major components of spearmint oil that elicited response in L. delicatula antennae, indicating the potential role of the essential oil as attractant that determine the choice of the attraction material. In the field test, spearmint oil exhibited attraction effect up to 5 days. This effect was different in accordance with test places and treatment dose.
207.
2010.05
구독 인증기관·개인회원 무료
Effect of Electron-beam Irradiation on the Development and Reproduction of Agricultural Insect Pests
On the development and reproduction of four major agricultural insect pests including B. tabaci, M. persicae, P. xylostella and T. urticae, electron-beam was irradiated with different doses of 30, 50, 70, and 100 Gy. The effect of electron beam was investigated with respect to the longevity, egg hatching, emergence, and fecundity. Eggs hatching of B. tabaci, P. xylostella and T. urticae were more inhibited as increased irradiation doses. Especially B. tabaci and T. urticae eggs were perfectly inhibited to hatch at a dose of 100 Gy. However, these pests showed no lethal effect on the nymph/larva, pupa, and adult stages. When irradiated on the eggs of B. tabaci, P. xylostella and T. urticae, the emergence was inhibited from nymphs/larvae to adults. Individually, B. tabaci emerged adult did not lay eggs at a dose of 70 Gy. Besides, fecundity of P. xylostella emerged adult decreased at a dose of 100 Gy. On the contrary, irradiation did not affect the longevity of P. xylostella adult. When irradiated on B. tabaci, P. xylostella, Myzus persicae and T. urticae nymphs/larvae, emergence was inhibited at doses of 70 and 100 Gy, and decreased the fecundity and inhibited the hatching of laid eggs. However, the longevity of adults did not affect
208.
2010.05
구독 인증기관·개인회원 무료
Mamestra brassicae (cabbage moth) is a common European moth of the order Lepidoptera and the family Noctuidae. The larval stage is highly polyphagous and is known to feed on more than 70 species of host plants from 22 families, including Brassica species, lettuce, onion, potato, pea, tomato and apple. M. brassicae has become a significant pest also in Asia due to the damage caused to agriculturally and economically important Brassica crops. It is difficult to control M. brassicae using chemical insecticide because of its rapid development of resistance. The objective of our study, therefore, was the mass production and formulation of a local strain of M. brassicae nucleopolyhedrovirus-K1 (MabrNPV-K1) for the development of viral insecticide to control it. In production efficiency of MabrNPV-K1 using M. brassicae larvae, the mortality of the 3rd instar larvae was 100% when inoculated with 1.0 × 105 PIBs/larva and the yield of MabrNPV-K1 was maximal. Regarding the mortality, yield of polyhedra, inoculation doses and required time, the 1.0 × 104 PIBs/larva at 30°C was determined as optimal conditions producing polyhedra efficiently. To formulate MabrNPV-K1, feeding toxicities of various supplements including spreader and ultraviolet (UV) -protectant were determined. Tinopal UNPA-GX which is UV-protectants was effective for protection of polyhedra from UV and showed the increased mortality when added with 1% concentration. Other supplements did not influence significantly the mortality of MabrNPV-K1. Formulated MabrNPV-K1 with several supplements showed higher pathogencity than un-formulated MabrNPV-K1.
209.
2010.05
구독 인증기관·개인회원 무료
산림에 발생하여 피해를 주는 돌발해충의 조기진단을 위하여 유충발생 시기인 5월부터 9월까지 수목을 가해하고 있는 유충을 기주와 함께 채집하여 플라스틱 사육용기에 넣어 온도 23℃, 습도 70%, 명암 16L:8D이 일정하게 유지되는 항온항습실 조건에서 실내사육을 실시하였다. 기주는 냉장보관하면서 공급하였으며, 유충의 충태별 형태와, 생육기간 등 생활사를 조사하였다. 이번 조사를 통하여 채집된 유충은 총 192종으로 이 중 75종이 확인되었으며, 117종은 사육중 폐사되었거나 번데기상태로 아직 미확인되었다. 분류군별로 정리하면 나비목이 22과 170종으로 가장 많이 조사되었으며, 그 다음이 딱정벌레목, 벌목의 순으로 조사되었다. 나비목에서는 자나방과가 45종, 밤나방과가 35종으로 가장 많이 조사되었으며, 그 다음으로 명나방과, 잎말이나방과, 재주나방과, 독나방과의 순으로 조사되었다. 기타 분류군에서는 잎벌레와 잎벌 등 식엽성 해충이 대부분 조사되었다. 또한 채집․사육된 유충들을 기주별로 분석한 결과 총 41종의 기주식물이 조사되었으며, 그 중 참나무류가 21종으로 가장 많이 조사되었다. 그 다음으로 버드나무, 벚나무, 밤나무 등의 순으로 많았다. 그리고 2종 이상의 기주식물을 가해한 유충으로는 미국흰불나방, 사과독나방, 무늬독나방, 긴금무늬밤나방, 꿩비름집나방, 줄점불나방, 줄고운가지나방 등이 조사되었다. 앞으로 이러한 기주식물별 유충자료는 산림해충 동정에 중요한 자료로 활용할 것으로 판단된다.
210.
2010.05
구독 인증기관·개인회원 무료
Tae-Won Goo, Sung-Wan Kim, Yong-Bo Kim, Seong-Ryul Kim, Seung-Won Park, Seok-Woo Kang, Kwang Gill-Lee, O-Yu Kwon, Eun-Young Yun
For stable germline transformation, the promoter of B. mori cytoplasmic actin gene (BmA3) was used to ubiquitous expression of transgenes. Except for BmA3 promoter, promoters used to regulate gene expressionin all tissues and developmental stages of B. mori were not nearly developed. To identify more powerful promoter than previously reported BmA3 promoter (Mange et al., 1997), we introduced a new dot blot hybridization method, and isolated nine clones that show stronger dot signal compared to the control, BmA3by this method. Among these 9 clones, we focused on one clone which has high amino acid homology (94%) with heat shock protein 70 gene of Trichoplusia ni. This resulting positive clone, named bHsp70 (B. mori heat shock protein 70) was ubiquitiously expressed in tissues and developmental stage of fifth instar B. mori larvae,and stimulated bythermal and ER stress. As result of promoter assay using dual luciferase assay system, we found the highest transcription activity region (-1003/+147) in the 5'-flanking region of bHsp70 gene that has 264-fold more intensive promoter activity than BmA3 promoter. Moreover, transcription activity of bHsp70 promoter under heat shock condition (42 ℃, 4 hr) was increased over 2-fold than normal condition. Therefore, we suggest that bHsp70 promoter may be used more effective candidate for transgene expression in B. mori.
211.
2010.05
구독 인증기관·개인회원 무료
Seong Ryul Kim, Min Jee Kim, Seung Won Park, Tae Won Goo, Seok Woo Kang, Kwang Gill Lee, Jae Sam Hwang, Iksoo Kim
Attacin is a well-studied glycine-rich antibacterial protein in insect immune response, which has limitary antibacterial effect to some Gram-negative bacteria. A cDNA encoding the attacin gene was screened and isolated from the immunized larvae of the swallowtail butterfly, Papilio xuthus. The complete P. xuthus attacin cDNA is 949 nucleotides encoding a 250 amino acid precursor that contains a putative 18-residue signal peptide, a common 42-residue propeptide sequence and a presumed 190-residue mature protein with a theoretical mass of 19904.01 and a pI of 9.13. The putative mature protein of P. xuthus attacin showed 48%~52% and 24%~30% identity in amino acid sequences with that of lepidopteran and dipteran insects, respectively. The attacin transcript was induced at significant level after injection with bacterial lipopolysaccharide (LPS). Recombinant attacin was highly expressed in E. coli BL21 (DE3) cells by fusing with an N-terminal S-tag/thrombin cleavage site configuration protein to avoid the cell death during induction. The expressed fusion protein was purified by Ni-NTA immobilized metal affinity chromatography (IMAC). After desalting and cleavage with thrombin, the recombinant attacin was released and showed considerably antibacterial activity against Gram-negative bacteria, E. coli ML 35. Our results proved that this protein family with a potent antibacterial activity may play a role in the immune response of butterflies.
212.
2010.05
구독 인증기관·개인회원 무료
The Laodelphax striatellus(SBPH: Small Brown Plant Hopper) is known to be not overwintering in South Korea and migrate from China to Korea every year by now, but these days many evidence support the possibility of SBPH overwintering. These insects do considerable damage to the rice production in Korea. If they will come from China, identification of origin of migration is very important to make countermeasure against SBHP outbreak. So we want to know exact the route of long-distance migration of them. In this study, we have developed some microsatellite markers for basic population genetic analysis of SBPH. We will show the genetic characteristics of them
213.
2010.05
구독 인증기관·개인회원 무료
To increase the fruit quality of the Japanese apricot, 3 species of insects, Apis mellifera, Bombus terrestris and Osmia cornifrons were used for pollination in Japanese apricot field in Jinju and Sancheong province. And also economical effect for crops of Japanese apricot fruit pollinated with same insect was analyzed. This study was also conducted using 3 kinds of methods for pollination effect, natural condition, net-house and control method. Because blooming period of Japanese apricot was different from the other fruit tree(Blooming time of Japanese apricot shows always in the middle of the March), foraging behavior of insects used in this examination showed different tendency each other. Especially O. cornifrons was not adapted to wind and lower temperature compared with A. mellifera and B. terrestris in this field condition. Rate of fruit set pollinated with A. mellifera and B. terrestris was showed 14.9% and 13% respectively. But O. cornifrons was lower than another 2 kinds of insects with 6% of fruit setting rate. Total rate of fruit set of Japanese apricot showed lower tendency compared with other kinds of fruit tree with under 30%. Quality of fruit pollinated with A. mellifera and B. terrestris showed higher than O. cornifrons in natural field condition in fruit weight. Economical income of Japanese apricot fruit producted by A. mellifera showed highest with 185% of benefit effect, and B. terrestris with 143% compared with natural field condition.
214.
2010.05
구독 인증기관·개인회원 무료
Eun-Young Yun, Tae-Won Goo, Jae-Sam Hwang, Mi-Young Ahn, Nam-Jung Kim, Young-Il Yoon, Chee-Hun Song, O-Yu Kwon
To examine the expression profile of oxidative stress responsive genes in Spodoptera litura, we constructed a cDNA library from S. litura injected with hydrogen peroxide (H2O2). Using a microarray chip composed of 2,964 cDNAs, we screened gene expression at 1, 3, 5, 7, and 9 h post H2O2 injection. Data were clustered into 15 groups of genes that behave similarly across each time course. Seventy-three genes were identified as being at least 2-fold up- or downregulated after treatment with H2O2 in S. litura. We constructed expressed sequence tags (ESTs) for genes that changed at least 2-fold after treatment with H2O2. The functional classification of these ESTs based on Gene Ontology showed that the ESTs are rich in genes involved in oxidoreductase activity (5.7%), defense (14.3%), cellular process (22.9%), and development (17.1%).
215.
2010.05
구독 인증기관·개인회원 무료
Jae-Sam, Hwang, Bo Ram Kang, Ho Kim, Eun-Young Yun, Sung-Hee Nam, Seong-Ryul Kim, In-Woo Kim, Mi-Young Ahn, Jong Soo Chang
Our previous study demonstrated that Coprisin, a peptide from Copris tripartitus infected with bacterial pathogens, has an antibacterial activity. We assessed in this study whether Coprisin caused cellular toxicity in various mammalian cell lines. Coprisin selectively caused a marked drop of cell viability in Jurkat T cells, U937 cells and AML-2 cells belonging to the human leukemia cells but not in Caki cells and Hela cells. Fragmentation of DNA, a maker of apoptosis, was also confirmed in theleukemia cell lines but not in other cells. The Coprisin-induced apoptosis in leukemia cells was mediated by AIF (apoptosis inducing factor), a caspase -independent pathway.
216.
2010.05
구독 인증기관·개인회원 무료
배 병해충 방제시 농약사용 절감과 방제효율 개선을 위해 고속살포기(SS기; Speed Sprayer; HA-1000SCA)를 사용하여 2개 시험포장을 대상으로 각 포장에 동일한 시험구로 관행구(노즐:ø1.0mm 표준노즐; 분무압력 : 20kg/cm2; 표준농도; 살포량 : 1L/3.3m2), 관행저물량구(노즐:ø1.0mm 표준노즐; 분무압력 : 20kg/cm2; 표준농도; 살포량 : 0.7L/3.3m2), 저물량-1구(노즐:ID노즐(Lechler사; 스위스); 분무압력 : 10kg/cm2; 1.5배 고농도; 살포량 : 0.5L/3.3m2), 및 저물량-2구(노즐:ID노즐 (Lechler사; 스위스); 분무압력 : 10kg/cm2; 1.5배 고농도; 살포량 : 0.7L/3.3m2)로 설정하여 방제하였는데, 시험포장에 따라 병해충의 발생 정도는 달랐지만 배 생육기 동안 검은별무늬병, 붉은별무늬병, 꼬마배나무이, 사과애모무늬잎말이나방의 방제 효과는 관행구와 저물량-2구에서 적은 병해충 발생량을 보였다. 관행저물량구와 저물량-1구의 병해충 발생량의 전자의 두 처리구보다 많은 경향을 보였다. 수확과실에 대해 검은별무늬병과 붉은별무늬병의 피해과율은 재배기와 유사한 경향을 보였지만 복숭아순나방 등 심식충류와 사과애모무늬잎말이나방의 피해과율은 처리구간 큰 차이가 없었고, 시포험포장 I과 II의 관행저물량구에서 깍지벌레류에 의한 피해과율이 각각 2.1과 7.5%로 가장 많았고, 다른 처리구에서는 0.9~1.4%의 피해과율로 큰 차이가 없었다. 따라서, 단위면적당 농약사용량(A.I.)은 일정하게 유지하여야 할 것으로 생각된다.
217.
2010.05
구독 인증기관·개인회원 무료
Bee venom contains a variety of peptides and enzymes, including serine proteases. Here we describe the molecular cloning and characterization of a serine protease (Bt-VSP) isolated from the venom of the bumblebee Bombus terrestris. The Bt-VSP gene consists of six exons encoding a 358-amino acid protein. The form of Bt-VSP detected in bee venom was the 34-kDa mature protein, which is created by cleavage of the catalytic domain of Bt-proVSP between Arg111 and Val112. Bt-VSP activates prothrombin and directly degrades fibrinogen into fibrin degradation products, defining roles for Bt-VSP as a prothrombin activator, a thrombin-like protease, and a plasmin-like protease. The finding that Bt-VSP acts as a fibrin(ogen)olytic enzyme is similar to a previous finding that Bi-VSP, a venom serine protease of B. ignitus, exhibits fibrin(ogen)olytic activity. We also compared major venom components in honeybee and bumblebee, and found that bumblebee venom contains a larger amount of serine protease. Furthermore, unlike bumblebee venom, which exhibits fibrin(ogen)olytic activity owing to the presence of a serine protease, it is likely that honeybee venom lacks fibrin(ogen)olytic activity.
218.
2010.05
구독 인증기관·개인회원 무료
Peptidoglycan recognition proteins (PGRPs) are pattern recognition molecules of the innate immune system that recognize peptidoglycan, a unique cell wall component of bacteria. Here we cloned and characterized PGRP-S from the bumblebee Bombus ignitus (BiPGRP-S). The BiPGRP-S gene consists of four exons encoding 194 amino acid residues. Comparative analysis indicates that the predicted amino acid sequence of BiPGRP-S shares high identity with enzymatically active PGRP-S proteins and contains the amino acids required for amidase activity. BiPGRP-S in B. ignitus worker bees is constitutively expressed in boththe fat body and epidermis, and it is secreted into the hemolymph. Quantitative real-time PCR assays revealed that in both the fat body and epidermis, the BiPGRP-S gene is highly induced by an injection of Bacillus thuringiensis. In addition, recombinant BiPGRP-S expressed as a 19-kDa protein in baculovirus-infected insect cells can bind to B. megaterium and B. thuringiensis but not to Staphylococcus aureus, Escherichia coli or Beauveria bassiana. Consistent with these data, BiPGRP-S shows antibacterial activity against B. megaterium and B. thuringiensis. These results indicate that BiPGRP-S is an inducible protein that may be involved in the immune response against bacterial infection of the genus Bacillus as an amidase-type PGRP-S.
219.
2010.05
구독 인증기관·개인회원 무료
Nam Sook Park, Han Seok Kang, Seon Ku Kim, Yong Gyun Kim, Byung Uuk Cho, Teak Soon Shin, Keun Ki Kim, Hyun Chul Park, Hong Joo Son, Hong Gu Lee, Sang Mong Lee
Fungi belonging to the Paecilomyces spp. have recently been used as food and herbal medicines in Korea and are greatly popular as commercially available powdered supplement or dried fruiting body. Despite this acceptance and its use, little is known of the genes related to its reactive agents. Presently, We have constructed an olig-d(T) primed directional cDNA library from the silkworm Dongchunghacho, an entomopathogenic fungus, of which species is belonging to Paecilomyces spp. based on the previous identification of ITS1 and ITS2 at the molecular level and collected from Jocheon Miryang, Korea. To isolate and screen genes in the fungus, 626 expressed sequence tags(ESTs) were generated by a partial sequencing from the cDNA library. cDNA encoding the glyceraldehyde-3-phosphate dehydrogenase(Pt-GAPDH) of Paecilomyces tenuipes- Jocheon was cloned from the above cDNA library. The complete cDNA sequence of Pt-GAPDH is comprised of 1,014bp encoding 338 amino acid residues. The deduced protein sequence of Pt-GAPDH showed higher homology with Beauberia bassiana-GAPDH(93% amino acid identity). Hydropathy analysis revealed that Pt-GAPDH protein is hydrophilic. The major three amino acids in its composition of amino acid residues were alanine(11.54%), valine(9.47%) and glycine(8.88%). The Pt-GAPDH gene of Paecilomyces tenuipes entomopathogenic fungus consisted of three exons and two introns coding for 338 amino acid residues, and the genomic DNA length of the gene spans 1302bp. The accession number of the gene in GenBank are GU997099 for Pt-GAPDH cDNA and GU997102 for Pt-GAPDH genomic DNA. More investigation works including gene expression, immunological analysis etc. will be carried continuously without hesitation after this presentation.
220.
2010.05
구독 인증기관·개인회원 무료
To search for novel biologically active venom components, secretory venom proteins of two solitary hunting wasps, Orancistrocerus drewseni Saussure (1857) and Eumenes pomiformis Fabricus (1781), were identified by SDS-PAGE in conjunction with mass analysis with the aid of venom gland and sac-specific EST libraries constructed by suppression subtractive hybridization. Arginine kinase was the most predominant protein in both wasp venoms. Along with the full-length arginine kinase, a truncated form, which was known to have paralytic activity on a spider, was a common predominant protein in the two wasp venoms. Insulin/insulin-like peptide-binding protein was abundantly found only in E. pomiformis venom and the EST library, which might be due to its unique behaviors of oviposition and provision. It seemed that some venom proteins are secreted into venom fluid from venom gland cells via exosomes, not by signal sequence-mediated transport processes. Amphipathic α-helical peptides (10-15 amino acids) were predominantly transcribed in the venom gland/sac than protein components, and showed cell lytic activities against insect cells, mammalian cells, bacteria, and fungi. Phospholipase A2 and hyaluronidase, which are known to be the main components of wasp venoms, were found in both wasp venoms. In addition, a dendrotoxin-like peptide known to be a K+ channel blocker was also found in the venom of E. pomiformis.
