간행물
한국응용곤충학회 학술대회논문집
- 발행기관 한국응용곤충학회
- 자료유형 학술대회
- 간기 반년간
- 수록기간 2000 ~ 2024
- 주제분류 농수해양 > 농학 농수해양 분류의 다른 간행물
- 십진분류KDC 495DDC 595
권호리스트/논문검색
2014 한국응용곤충학회 추계학술발표회 (2014년 10월) 269건
241.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.250
한국응용곤충학회
식물 생육의 장애요인으로는 물, 온도 그리고 영양분 등의 환경적 요인들과 식물 을 가해하는 해충과 여러 가지 식물병 등의 생물적 장애요인이 있다. 생물적 장애요 인인 식물병과 해충을 방제하기 위해서 다양한 살균제와 살충제 등의 작물보호제 가 활발히 연구 개발·이용되고 있으나 이러한 작물보호제는 막대한 경제적 비용이 발생하고 특히, 최근에는 이들 작물보호제의 환경 위해성 문제와 더불어 인간에게 안정한 농산물 생산의 중요성이 강조되면서 병·해충의 친환경 방제법이 확대 실시 되고 있다. 그리하여 본 연구에서는 식물병과 해충을 동시방제하기 위한 친환경소 재로써 곤충병원성 곰팡이를 이용하여 그 가능성을 연구하였다. 대표적인 난방제 해충인 점박이응애와 복숭아혹진딧물의 병원성 곰팡이 29개를 이용하여 식물병 원성 세균 Ralstonia solanacearum과 곰팡이 Botrytis cinerea에 대한 항균활성을 고체배지에서 조사한 결과, 전체 실험 균주 중 약 83%(24개)와 69%(20개)에 해당 하는 균주가 각각 R. solanacearum과 B. cinerea의 생장을 서로 다른 능력으로 억제 하는 것을 관찰 할 수 있었다. 또한, 고체배지에서 항균활성이 확인된 곤충병원성 곰팡이 균주의 배양액은 그 자체만으로도 각각의 식물병원균에 대해 항균활성이 있는 것을 확인하였다. 본 연구에서 항균활성이 확인된 곤충병원성 곰팡이 균주들 은 식물의 해충과 식물병을 동시에 방제하기 위한 연구의 재료로 유용하게 쓰일 수 있을 것으로 기대된다.
242.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.251
한국응용곤충학회
저곡해충 중 대표적인 거짓쌀도둑거저리(Tribolium castaneum), 어리쌀바구미 (Sitophilus zeamais), 화랑곡나방(Plodia interpunctella), 이질바퀴(Periplaneta americana)에 대한 열처리 방제시스템에 훈증제(Phosphine), CO2, 식용규조토를 복합처리하여 식품가공공장, 사일로, 컨테이너 등에 대한 현장적용시스템을 개발 하였다.
식품가공공장의 해충 주요 서식처는 공장설비 내부 혹은 바닦면의 작은 틈새로 써 대기 온도뿐만 아니라 생산설비와 건물 표면의 온도가 최소 50℃이상 열평형을 이루기 위해서는 약 24시간 이상의 온도 관리가 필요하였다. 또한 현장에서 열처리 시 살충효과 개선 및 방제시간 단축, 그리고 방제온도 감소를 위해서 4종 해충을 4 0℃ 및 45℃ 조건에서 Phosphine 50ppm, CO2 2%, 규조토 0.5g/m3 복합처리하여 사멸율(LT100)을 조사하였으며, 거짓쌀도둑거저리의 경우 약 36시간(45℃ 단독 처리)에서 9시간(45℃ + Phosphine + CO2 + 규조토 복합처리)으로 방제시간이 급 격히 단축되었다. 열처리와 함께 복합처리된 Phosphine과 CO2의 경우 가스형태로 공장 밀폐도가 부족하여 1일 약 10∼20%정도의 농도가 감소하였다. 저장곡물 보 관용으로 주로 사용되는 사일로는 일반적으로 직경 약 2m이상이고 높이 약 10∼ 25m정도의 원기둥 형태로써, 해충의 주요 서식처는 상부 여과장치 부분이다. 이 경 우 열방제 장비를 사일로 하단 내부에 두고 열풍의 토출부를 사일로 상부에 위치하 여 방제를 수행할 경우 효과적이였다. 또한 수출입 콘테이너의 경우 식품가공공장 과 달리 구조물의 밀폐도가 우수하였으며, 이 경우 Phosphine 및 CO2와 같은 가스 의 적용이 효과적인 것으로 확인되었다.
243.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.252
한국응용곤충학회
Behavioral reaction can be measured as a sensitive endpoint for sublethal toxicity of copper, and can be used to obtain easily and quickly. Also behavioral endpoints may serve as a more insightful evaluation tool of the ecological effects of toxic chemicals.
In this study, four invertebrates in freshwater which are usually used indicate species for water quality were selected as test species (Chironomus riparius, Heterocypris incongruens, Daphnia magna, and, Triops longicaudatus). Each test species was exposed to copper for 6 hours, and total distance, velocity, and, turn angle were measured for 1 hour using video analysis system (Ethovision : Noldus Information Technology) in laboratory condition.
Each endpoints reflected effect of copper toxicity appropriately for all test species. These endpoint have possibility that can be used to identify characteristic behavioral responses to a metal toxicity.
We viewed this study as a preliminary experiment for future research to investigate the significance of behavioral endpoints to various toxic chemicals.
244.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.253
한국응용곤충학회
중부지방 회화나무 가로수 및 조경수에 발생하여 피해를 주고 있는 줄마디가지 나방(Chiasmia cinerearia)은 분류학적으로 자나방과(Geometridae) 가지나방아 과(Ennominae)에 속한다. 줄마디가지나방은 주로 잎 위에 250∼300개의 알을 무 더기로 산란하였으며, 유충은 총 5령기를 거쳐 용화되었다. 성충은 6월중순과 8월 경에 2회 우화하였으며, 1세대 경과일수는 약 48일로 조사되었다. 유충의 몸길이 는 25∼30mm정도로 연녹색을 띠고 복부의 옆선은 황색이었다. 성충의 날개편 길 이는 36mm정도로 회갈색을 띠며 앞날개 외횡선을 중심으로 4각형의 무늬가 무리 지어 있는 것이 특징이다.
245.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.254
한국응용곤충학회
지속적인 대형 계발사업에 노출되어 있는 수도권 서부의 습지 주변 6개 비오톱 에 서식하는 육상곤충을 대상으로 2005년부터 2013년 까지 총 9년간 장기조사를 수행하였다. 조사지역의 행정구역은 경기도 시흥시 및 화성시 일대였으며, 습지 주 변 비오톱 6곳을 지정하여 조사지역으로 선택하였다. 조사시기는 4, 6, 8, 10의 2개 월 간격을 최대한 유지하였으며, 매회 스위핑(sweeping)을 활용한 주간조사를 하 였고, 6월 조사에서는 1개 지점에서 유아등과 스크린을 이용한 야간채집을 함께 수 행하였다. 2005년 총 10목 76과 255종 출현을 시작으로, 2006년 10목 83과 273종, 2007년 13목 86과 282종, 2008년 13목 110과 354종, 2009년 12목 107과 338종, 2010년 10목 97과 295종, 2011년 12목 106과 337종, 2012년 11목 75과 220종, 2013년 9목 85과 232종이 출현하였다. 2005년부터 연도별 출현종수를 비교하면 2005년 10목 76과 255종으로 시작해서, 2008년 13목 110과 354종으로 최대종수 를 기록하였고, 이후 감소추세가 이어지고 있다. 본 연구는 향후 기후변화 및 생물 군집 연구에 큰 도움이 될 것으로 판단된다.
246.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.255
한국응용곤충학회
The Wolbachia bacterium is one of the best studied reproductive manipulators found in variuos invertebrate taxa. Theoretically it infects two thirds of invertebrates. It is thought to have a huge impact on its hosts’ evolution.
The ant is a very successful organism in terrestrial ecosystems especially with its charicteristic sociality. However, the influence of Wolbachia on the animal remains to be paid more attention.
This study is to examine Wolbachia infection frequency in some ant species in Korea. The result shows that 9 out of 12 ant species harbor the bacterium. Furthermore, at least two species show polymorphism in their infection status at the colony level.
This will be used as a foundation of further study on the interactions between the bacterium and ant species.
247.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.259
한국응용곤충학회
The Riptortus pedestris-Burkholderia symbiotic system is a promising model for understanding molecular mechanism of symbiosis. In previous studies, the Burkholderia symbiont has been shown to play important biological roles in the growth and fitness of host R. pedestris. The Burkholderia symbiont, one of bacteria found in the soil, is the only bacterium that can colonize the symbiotic midgut region of R. pedestris. However, the molecular mechanism of host selectivity for the Burkholderia symbiont remains unknown. To determine where the selection occurs, we firstly compared initial infectivity of different mid-gut regions after oral infection of Escherichia coli and Burkholderia. Interestingly, E. coli were not detected in any regions of mid-gut, while Burkholderia could reach to the posterior region of mid-gut. Therefore, we hypothesized that host selectivity for the Burkholderia symbiont is occurred in the salivary gland. To address this hypothesis, we treated E. coli and Burkholderia with lysate of salivary gland and examined their survival by estimation of colony forming unit (CFU) on the plate. We found that E. coli, but not Burkholderia, was susceptible to the lysate of salivary gland. To determine molecular basis of the selective mechanism in the salivary gland, we analyzed antimicrobial proteins (AMPs) from lysate of salivary gland. we identified three AMPs, namely rip-trialysin1, rip-trialysin2 and lysozyme and further purified rip-trialysin1 and rip-trialysin2. When E. coli and Burkholderia were treated with rip-trialysin1 and rip-trialysin2, rip-trialysin1 exhibited little antimicrobial activity, but rip-trialysin2 exhibited antimicrobial activity. Furthermore, we found that E. coli was susceptible, but Burkholderia is resistant to commerciallypurchased egg white lysozyme. Our results suggest that R. pedestris salivary gland provides a chance of selection for the Burkholderia symbiont and lysozyme in salivary gland seems to play an important role for the selection of gut symbiont.
248.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.260
한국응용곤충학회
Double-stranded RNA(dsRNA) had been used to specitically suppress target gene expression at post-tanscription level. Injection of dsRNA to hemocoel is the most efficient to knockdown target mRNA. However, some insects have shown to be susceptible to feeding dsRNA. Spodoptera exigua was susceptible to dsRNA at oral treatment. Especially dsRNA specific to β-integrin was potent to survival of S.exigua larvae. This study advanced our dsRNA application technology by generating recombinant E.coli expressing dsRNA specific the β-integrin. A recombinant vector L4440 was constructed with a partial β-integrin gene under T7 RNA polymerase promoter. The recombinant vector was used to transform HT115 competent cells of E.coli. The transformed E.coli expressed the dsRNA. The production of dsRNA was proportional to the bacterial number. By feeding the recombinant E.coli, S.exigua underwent significant mortality. By adding E.coli expressing Cry1Ca Bt toxin to E.coli expressing dsRNA, S.exigua exhibited highly enhanced mortality. This study suggests a possibility to use a recombinant E.coli expressing dsRNA to control S.exigua.
249.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.261
한국응용곤충학회
Like vertebrate insulins, insulin-like peptides (ILPs) play crucial roles in controlling immature growth, adult lifespan, and plasma sugar level in some insects. An ILP gene (SeILP1) was predicted from a transcription database of Spodoptera exigua. SeILP1 encodes 95 amino acid sequence, which shares sequence homologies (33~83%) with other insects ILPs. The predicted B and A chains possess six cysteine residences. SeILP1 was expressed in all developmental stages of S. exigua. However, its expression was detected in fat body, gut and epidermis, but not in hemocytes. Its expression increased with feeding activity. Plasma trehalose levels of fifth instar larvae maintained at relatively stable concentration of 2.31±0.62 mM. However, starvation induced a significant increase of plasma trehalose level by more than two fold in 48 h, at which SeILP1 expression kept at a low level. RNA interference of SeILP1 induced a significant increase of plasma trehalose level. Interestingly, a bovine insulin decreased plasma trehalose level in a dose-dependent manner. These results indicate mat SeILP1 plays a role in suppressing plasma trehalose level in S. exigua.
250.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.262
한국응용곤충학회
Cotesia plutellae, an endoparasitoids braconid wasp, possesses a polydnaviruses (PDVs) called Cotesia plutellae bracovirus (CpBV) that encodes viral histone H4 (= CpBV-H4). This viral histone H4 shares high sequence homology (82.5%) with host`s H4 of P.xylostella, except an extended N-terminal tail consisting of 38 amino acid residues with nine lysines. Its extended N-terminal tail has been postulated to play a crucial role in suppressing host immunity, growth and development-associated genes, presumably through an epigenetic control mechanism. A suppression subtractive hybridization (SSH) analysis was analyzed in transcriptome by short-read sequencing technology and provided several target and non-target genes of a viral histone H4. In this study, we analyzed the effect CpBV-H4 on the expression of two target genes: Lysine-specific demethylase (KDM) and Serine proteinase inhibitor (Serpins). Transient expression of CpBV-H4 into non parasitized P. xylostella was performed by microinjection of a recombinant expression vector, and showed the expression up to 70 h. Under this transient expression condition, we analyzed the effect of CpBV-H4 on the expression of target genes by RT-PCR at different time points. Interestingly, the CpBV-H4 significantly inhibited the expression of these target genes, while the truncated CpBV-H4 deleting the N-terminal tail did not show this inhibitory effect. This study also showed that the viral histone H4 suppresses expressions of lysine-specific demethylase and serine proteinase inhibitor (Serpin2) to inhibit host growth and development.
251.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.263
한국응용곤충학회
Mealworm, Tenebrio molitor L. (Coleoptera: Tenebrionidae) has high and safe protein contents, which enables it to be animal feed. However, occurrence of entomopathogenic fungi in mealworms is one of the limitations for mass production. In this work, we investigated relationships between abiotic conditions and occurrence of fungal pathogens and established an effective control method using fungicides. In virulence assay, third instar mealworm larvae were sprayed by six entomopathogenic Beauveria bassiana isolates and kept under high relative humidity; B. bassiana ERL1575 isolate had highest virulence. Under normal humidity, ERL1575 conidia showed different virulence between spray (~0% virulence) and digestion (~80% virulence) method. Furthermore, mealworms, which digested conidia, were exposed to various temperature (20-35°C) and humidity (1-3 ml distilled water spray/35 mm diam. dish) conditions for 5 days. All the treatments showed ~90% virulence except 35°C incubations (~20% virulence), but irrespective to the humidity conditions. Forty chemical fungicides were assayed against conidial germination and hyphal growth of ERL1575. Fluazinam and mancozeb showed strong inhibition of conidial germination at standard application dose (SD), 1/2 SD and 1/5 SD; besides, fluazinam showed strong inhibition of hyphal growth. When fluazinam and mancozeb were applied to the fungal conidia-inoculated wheat bran, most of mealworms were alive after 3 days post application. However, high mortality rate (~100%) were observed in the conidia-inoculated wheat bran without any fungicides. In conclusion, this work suggests that B. bassiana isolates could be pathogens at <30°C when they were digested by mealworms, and fluazinam and mancozeb would be used as effective control agents against the pathogen.
252.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.264
한국응용곤충학회
곤충 병원성 곰팡이 Metharizium anisopilae JEF 003, 004와 Beauveria bassiana JEF 006, 007의 대량생산배지 조건에 따른 열안정성을 평가하였다. 첫 번째로 millet 배지에서 배양된 포자의 열안정성 평가를 위하여 50℃ 조건에 0, 30, 60, 90, 120 min으로 포자 현탁액 상태와 grain상태로 노출한 결과 현탁액 상태에서 포자 의 열안정성이 더 많이 감소하는 것을 확인하였다. 다음으로 각기 다른 고체배지 (조: 1~5.×109 conidia/g, 수수: 1~2×109 conidia/g, 기장: 2~3×109 conidia/g) 조건에 서 생산된 포자의 열안정성 확인을 위하여 배양이 완료된 포자를 현탁액과 grain상 태에서 50℃ 조건에 0, 1, 2, 4, 8 hours동안 노출하여 열안정성을 평가하였다. 실험 결과 현탁액 상태보다 grain상태에서 포자의 열안정성이 더 높은 것을 확인하였으 며, 조 배지 조건에서 포자의 열안정성이 가장 높게 향상된 것을 확인하였다. 마지 막으로 포자의 열안정성의 추가 향상을 위하여 배양이 완료된 고체배지 포자에 cotton seed oil, coconut oil, soybean oil, castor oil, olive oil, mineral oil을 넣고, 5 0℃의 온도에 0, 1, 2, 4, 8 hours으로 열안정성을 평가하였다. 결과적으로 cotton seed oil, soybean oil, castor oil, olive oil을 처리한 포자에서 높은 열안정성을 확인 하였다. 따라서 곤충병원성 곰팡이의 열안정성 실험 결과로 확인 된 조를 이용하여 높은 열안전성을 확보할 수 있을 것으로 판단이 되며, 추가적인 열안정성 확보를 위 하여 식물성 오일을 제제에 이용할 수 있을 것으로 전망된다.
253.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.265
한국응용곤충학회
Climate change is a global phenomenon and has major impacts on ecotoxicology. A variety of environmental variables affected by climate change can alter the fate of chemical and responses of organism. Especially, soil temperature is an important factor in ecotoxicology. Increasing temperature results in an increase in the rate of uptake and degradation of toxic compound. Therefore, the research of temperature effect on toxicity is needed to understand the change of toxic effect under climate change.
In this regards, the response of Paronychiurus kimi (Collembola) to Geunsami™ (glyphosate-based herbicide) were evaluated at different temperatures (20℃, 25℃) and soil aging time (7, 15 days). Survived adults and hatched juveniles were counted after 28-day exposures in artificial soil spiked with 1, 5, 50, 100, 500 mg/kg of glyphosate in different temperature and soil aging time conditions. In addition, we investigated the fatty acid composition of Paronychiurus kimi.
Increasing soil aging time and temperature, EC20 value of P. kimi was increased. Fatty acid composition of P. kimi was similar with that of Folsomia candida mainly composed of 18:1 w9c, 16:0 and 18:0 fatty acids. UI (Unsaturation Index) and the ratio C16/18 of fatty acid composition decreased with increasing temperature. The 18:0 (Stearic acid) fatty acid increased with increasing concentration of glyphosate.
254.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.266
한국응용곤충학회
Understanding how species will respond to projected future climate change has become important. However, the impacts of climate change on the ecosystem are very complex and uncertain, we need a reliable tool for approaching it. Mechanistic modeling can be one of the solution for handling the various factors and responses of test organisms in regard to climate change. We introduce the case study on the copper toxicity on D. magna and show the applicability of these mechanistic model approaches.
The overall objective of this case study was to simulate the chronic toxicity of copper on Daphnia magna using dynamic energy budget theory with the improved toxicity module component. The toxicity module includes toxic effects on allocation of reserve, structure, and maturity energy in the D. magna. Model calibration and verification were performed using data sets obtained from a laboratory experiment that include growth, maturity and survival measurement data of D. magna during copper exposure. The simulation results show that the response of D. magna under copper exposure was well estimated by toxicity module. Overall, the results show the dynamics model based on DEB theory can be used for estimating long-term metal toxicity on D. magna. Thus, mechanistic modeling can be utilized as a approach tool for evaluating the impacts of climate change on the ecosystem with more mechanistic description.
255.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.267
한국응용곤충학회
Winter cherry bug, Acanthocoris sordidus Thunberg (Hemiptera: Coreidae) is a pest of Solanaceous and Convolvulaceous crops and distributed in Taiwan, Japan, and Korea. Adult female generally lays eggs in cluster on host plant leaves. Most researches have been conducted in Japan, and it is regarded as a minor pest. In this study, we surveyed the seasonal population change of A. sordidus and its damages from May to September by visual count in experimental pepper fields located in Andong National University, where no pesticide was sprayed. Hatchability was measured using field-laid egg mass covered by nylon-meshed pouch. Potential disease transmission was confirmed in filed pepper fruits confined for a week with two adult A. sordidus inside nylon-meshed pouch. Adults and eggs of A. sordidus started to appear in 30th May and 10th June, respectively. Nymph populations were first found on 10th July. No eggs were found from 7th September when highest adult population (67/plant) was recorded. Hatchability was over 90% in the field. No disease transmission to pepper fruit was observed. We believe that A. sordidus would become a serious pest under a certain condition such as low level of insecticide use and climate change.
256.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.268
한국응용곤충학회
Chemical control is the indispensable part of insect pest management. However, due to overuse of pesticides, the emergence of resistance to pests and environmental pollution problems are appeared. In order to solve these problems, the need of integrated pest management (IPM) has emerged. The IPM means that the pest density should be controlled and maintained to avoid any economic loss, also to minimize any side effects to the human beings and environment using appropriate control methods. In order to do successful IPM, sampling plan should be prepared. Sampling plan consists of monitoring, density estimation and decision making of target insect pests in certain crop field including the knowledge of spatial distribution.
Tabaco whitefly, Bemisia tabaci, and Western flower thrips, Frankliniella occidentalis, are serious insect pests in paprika greenhouses. We selected two paprika greenhouses with different sizes (12,000 m2 and 4,000 m2). Insect pests monitoring was evaluated from January 24, 2014 to June 27, 2014. Two monitoring methods are used to develop sampling plans, one is visual survey for B. tabaci population, and the other is using sticky trap for F. occidentalis population, respectively. We calculated spatial distribution using Taylor’s Power Law (TPL). In visual survey results, TPL showed that adults and pupae of B. tabaci were aggregated in paprika greenhouses. And in trap survey results, F. occidentalis was also aggregated with different scale of the slope of TPL equation. In density estimation, the more density of insect pests, the less samples were required. Moreover, the more accuracy, the more samples were required for stop sampling in greenhouses. We selected hypothetic action threshold of 0.3 and 5 for decision making. As a result, if the action threshold is increased, the maximum sample number is decreased
257.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.271
한국응용곤충학회
Enhanced green fluoresce protein gene (egfp) was expressed in Beauveria bassiana ERL836 based on the Agrobacterium tumefaciens-mediated transformation (AtMT) method in this study. The ERL836 transformants were generated with pCambia-egfp binary vector. Ten transformants were randomly selected and analyzed for the T-DNA insertion and gene expression. The results revealed that 60% of the fungal putative transformants were inserted by the T-DNA fragment. Of these transformants, 33.33% (2 transformants) expressed the egfp gene. The egfp transformants showed strong green fluorescence with different expression levels. The results of this study could provide a reference for foreign protein expression in B. bassiana by using the AtMT method.
258.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.272
한국응용곤충학회
The baculovirus expression vector system (BEVS) is an effective and widely used method for the production of recombinant proteins in insect cells or larvae. However, the expression efficiency of foreign proteins using the polyhedrin promoter could not obtain the protein yields observed for native polyhedrin. To enhance the production efficiency of foreign protein in baculovirus expression system, the effects of various polyhedrin fragments were investigated by fusion expressing them with the enhanced green fluorescent protein (EGFP). Among the fusion-expressed protein in nucleus and cytoplasm, the most hyper-expression was observed in the fusion of amino acids 19 to 110 and 32 to 59 of polyhedrin. Additionally, the several proteins expressed by the partial polyhedrin-fused expression system was markedly increased. However, we identified that hyper-expression of target protein varied depending on the partial polyhedrin. Therefore, we constructed the virus inducible partial polyhedrin fusion transient expression system. This system amenable for screening of suitable partial polyhedrin to produce the target protein. The present study suggests a new option for higher expression of useful foreign recombinant protein using the partial polyhedrin fusion expression in baculovirus.
259.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.273
한국응용곤충학회
The most industry players were still building a case for biopesticides as an important sector of the crop protection industry, most bigger players were making guarded statements about their intentions in the sector, and the headline proclaimed that biopesticides were no longer a fringe sector. Within months, the larger companies started getting seriously involved through acquisitions and licensing deals. The second special issue that came out in 2013 gauged the momentum that the sector quickly achieved and it was becoming clear that biopesticides would figure in the plans of most of the big crop protection companies. The momentum that started in 2012 was sustained throughout 2013 and the first four months of 2014 in the forms of deals as well as acquisitions. The Europe-based majors consolidated the acquisitions made during the previous year and went about submitting registration dossiers for the acquired products and formulating business plans incorporating biopesticides into their overall strategy. In 2013, big US-based companies became active. Monsanto acquired US agricultural biotechnology company, Agradis, which has a collection of thousands of plant-associated microorganisms; formed a partnership with Danish company Novozymes to develop and commercialise biopesticides worldwide; and supported the establishment of a US company, Preceres, aimed at the development of delivery methods for RNAi-based biopesticides. The global companies believe that biologicals will provide new growth opportunities in agriculture and are looking at market opportunities in all crops and the needs of its crop protection business.
260.
2014.10
구독 인증기관·개인회원 무료
한국응용곤충학회 학술대회논문집
응용곤충학의 현안 - 검역과 곤충산업 Frontiers in Applied Entomology - Quarantine and Insect Industry
p.274
한국응용곤충학회
곤충병원성선충은 지상부와 지하부 서식 해충에 모두 병원성을 나타내지만 토 양을 서식처로 하고 있는 천적자원이다. 따라서 곤충병원성선충의 탐색은 주로 토 양시료를 채취하여 미끼곤충을 투입하는 방법을 주로 사용하면 때대로 토양 내에 서 치사 된 감염치사충으로부터 분리하기도 한다. 우리나라에서 곤충병원성선충 자원의 탐색은 1990년대 초에 최초로 이루어져 499개의 토양 시료 중 23개 지역 토 양에서 곤충병원성선충을 분리한 바 있고, 이후 2000년과 2010년대 초반에 전국단 위 조사가 수행되어 세계적으로 2과 76종의 곤충병원성선충들 중 우리나라에서는 2과 6종 30여 계통이 채집되고 있다. 이러한 채집 된 곤충병리자원들은 체계적인 관리가 제한적으로 이루어지고 있다. 따라서 국내 토착 생물자원의 탐색과 유용생 물자원의 확보적인 측면에서 곤충병원성선충을 비롯한 곤충병리 생물자원의 국가 단위의 체계적 발굴과 보존에 대한 협업과 시설 확보가 절실한 실정이다.
