간행물

한국응용곤충학회 학술대회논문집

권호리스트/논문검색
이 간행물 논문 검색

권호

2015 한국응용곤충학회 정기총회 및 춘계학술발표회 (2015년 4월) 238

심포지엄 Ⅱ

41.
2015.04 구독 인증기관·개인회원 무료
종자류를 가해하는 해충에서 저장곡물처럼 가해하는 해충의 종류는 매우 많지만, 기주 특이적으로 가해하는 경우는 콩바구미류와 좁쌀바구미류와 씨살이좀벌과가 대표적이다. 이 중, 종자의 내부를 가해하며, 밖에서 볼 때 잘 표시가 나지 않는 씨살이좀벌과의 해충은 재식용으로 종자를 통해 쉽게 이동할 수 있다. 그 동안 종자를 통해서 국내에 침입한 해충으로 알려진 해충은 콩바구미과의 팥바구미, 완두콩바구미 등이 있으며, 씨살이좀벌과에서는 현재 알려진 종으로는 복숭아씨살이좀벌 Eurytoma maslovskii이 국내 매실에 큰 피해를 주고 있다. 종자류에 피해를 주는 씨살이좀벌류는 처음에는 잘 인식되지 않지만, 밀도가 높아져 피해를 주기 시작하면서 주요 생산물이 종자와 열매에 직접 피해를 주기 때문에 큰 피해를 줄 우려가 있다. 검역 상 검출된 씨살이좀벌류는 2000년 이후 총 16건이었으며, 수입국으로는 자작나무 종자 1건만 몽골이고, 15건은 중국이었다. 검출된 종류로는 콩과식물인 아카시아와 한약재용 종자에서 Bruchophagus sp. 가 6건이 기록되었으나 실제 정확한 종으로 동정되지 않았다. 나머지 6건은 Eurytomidae 과로 동정되었으며, 검출된 식물은 복숭아, 황기, 십자화과엽경채류, 자작나무, 한약재류등 5개 품목이었다. 실제 종 수준의 씨살이좀벌 동정이 이루지지 않았지만 기주 특이성이 있는 것으로 볼 때, 4종 이상의 종이 검역 과정에서 검출된 것으로 볼 수 있다. 실제 종자류의 검사 시 피해를 입은 종자도 성충이 빠져나오기 전에는 외관상으로 건전종자와 거의 차이가 없어서 구분이 어려워, 기형 또는 약간의 변색이 있는 종자류를 집중적으로 검사해야 할 것이다.
42.
2015.04 구독 인증기관·개인회원 무료
Internal sperm storage after mating is important for insect reproduction, because it permits delayed fertilisation, and post-copulatory mate choice in polyandrous females. The polyandry is common in many animal taxa including insects, because it increases female fitness by reducing the risk of infertility and providing opportunities for sperm competition and choice. The reproductive success of males, on the other hand, often depends upon avoidance of sperm competition by preventing mated females from copulating and receiving sperm from other male suitors. A widespread strategy used by males is the use of the male seminal fluid proteins (SFPs) that form the mating plug and alter female behaviors, for example by suppressing mating receptivity and elevating egg-laying. Under these circumstances, females are expected to evolve mechanism(s) to control exposure to the male SFPs in order to maximize fitness by balancing the positive and negative impacts of polyandry. Here, we discover that Drosophila melanogaster females eject male ejaculates 1-6 h after mating with a stereotypic behaviour, and that this is regulated by a brain neuropeptide pathway composed of diuretic hormone 44 (Dh44), and its receptor Dh44R1. We showed that suppressing Dh44 or Dh44R1 signals in the brain expedites sperm ejection, whereas enhancing Dh44 or Dh44R1 signals delays sperm ejection. This study uncovers a molecular mechanism by which females can influence sperm competition and selection, and counter actively the negative impact of polyandry.
43.
2015.04 구독 인증기관·개인회원 무료
Although it is believed that internal nutrient sensors play important roles in feeding behaviors, their molecular and neural mechanisms underlying of the modulation of physiological status and cell growth are poorly understood. Using a Ca2+ imaging experiments with heterologous expression systems, we show that one of the gustatory receptors in the western honey bee Apis mellifera is selectively tuned to amino acids. Remarkably, we report that this gustatory receptor of the honey bee is highly expressed in hypopharyngeal gland, which plays a role in caste differentiation as well as royal jelly production and secretion. Knocking down this gustatory receptor gene reduces cellular pathways responsible for nutritional sensing such as mTOR signals in hypopharageal gland. Furthermore, the interfering expression of this gustatory receptor gene not only alters morphological changes and developmental retardation of the hypopharyngeal gland, but it also blocks cellular growth signals to induce autophagy. This new report indicates that internal sensing and downstream signals detecting nutrients is essential for honey bee to maintain the cellular growth and development of internal organs essential for caste development and maintenance of social structure in the honey bee.
44.
2015.04 구독 인증기관·개인회원 무료
현대 곤충분류학은 분자생물학적 방법론의 발달과 함께 종(species)과 집단(population)의 경계 해석에 대한 새로운 도전에 직면해 있다. 과거의 분류학자들은 대부분 형태, 생태, 지리 정보에 국한되어 종을 기재하고 분류하였는데, 조사지역과 표본수집의 한계 때문에 중복기재에 의한 동물이명 문제가 빈번하게 나타났다. 이러한 분류학적 오류를 바로 잡기위해 분류학에도 분자생물학적 연구방법이 활용되었고 수많은 분류학적 난제들이 해결되었다. 특히, DNA barcoding은 혁명이라고 해도 과언이 아닐 정도로 2000년대 중반부터 대부분의 분류군에 폭넓게 사용되어 10여년이 지난 현재 분류학에서 상당히 보편화된 방법으로 인정받고 있다. 하지만 곤충에서의 빠른 분화속도를 감당하기에 미토콘드리아 DNA의 일부분을 연구하는 것으로는 불충분하였다. 또, 곤충은 아종, 품종, biotype 등의 구별이 어려워 종과 집단의 경계에서 이를 명확하게 해결해줄 만한 접근법이 필요하였다. 이러한 문제를 극복하고자 최근에 연구자들은 집단유전학을 활용하여 집단 또는 종의 분화 과정을 이해하려고 노력하고 있으며 그 활용분야가 점차 확대되고 있다. 본 소모임에서는 그동안 연구결과 소개를 중심으로 분류학에서의 집단유전학 활용과 곤충 집단의 분화 현상에 대한 이해를 도모하고자 한다.
45.
2015.04 구독 인증기관·개인회원 무료
The development and fitness of insects depend on the temperature to which they are exposed. The thermal windows are defined as the temperature range between the minimum and maximum rate of development for individual species. The intrinsic optimal temperature for development proposed by Ikemoto is one of important factors that determine the fitness of optimum life history. The temperature requirements for development of 65 species from five orders of insects was obtained from several journals. The minimum and maximum rate of development was estimated using empirical models. The temperature tolerance range of enzyme activation was estimated using Shape-Schoolfield-Ikemoto (SSI) model. The mean and range of intrinsic optimal temperature were 20.89°C and 15.7~27.7°C. The mean intrinsic optimal temperatures of Hemiptera and Endopterygota (Coleoptera, Diptera, Hymenoptera and Lepidoptera) were 20.97°C and 20.71°C. The mean and range of thermal windows were 25.59°C and 16.69~36.13°C. The mean thermal windows of Hemiptera and Endopterygota were 25.53°C and 25.62°C. also not much different. Each species of insects had a limited temperature range for development. It is needed further studies for understanding the ecological, physiological and evolutionary response of insects to their thermal environments.

구두발표

46.
2015.04 구독 인증기관·개인회원 무료
Pogonini Laporte, 1834 is one of the tribes in Trechinae Bonelli, 1810 (Coleoptera: Carabidae). This tribe is morphologically similar to Trechini, Benbidiini, Zolini and Patrobini of the subfamily. However, Pogonini can be distinguished from the other tribes by having a combination of the following characters: apical maxillary palpomere fusiform; umbilical series of setae on elytron more or less contiguous (though sparse in middle); each tarsomeres with a median sulcus on dorsal side. Pogonini includes about 85 species of 12 genera worldwide and is found in all zoogeographical regions of the world. Many species inhabit along the sea coasts or near salt lakes, and are recognized as halobiont. In Korea, Paik (1998) reported Pogonus (Pogonus) itoshimaensis Habu, 1954. However, he added only the species name in the checklist without description or illustration although few specimens were examined. After Paik’s study, any studies on taxonomic status and distribution of Korean Pogonini have not been carried out. The genus Pogonus Dejean is the largest group in Pogonini with two subgenera: P. (Pogonoidius Carret, 1903) and P. (Pogonus Dejean, 1821). These two subgenera share very similar external morphological characters; Pogonoidius has setae on anterior angles of pronotum and setae at 7th interval of elytra while Pogonus does not have them. In the present study, two species, Pogonus (Pogonus) japonicus Putzeys, 1875 and P. (Pogonoidius) punctulatus Dejean, 1828, are newly recognized from Korea. A key to the Korean Pogonini species, redescription, distribution, photos of diagnostic characters, and illustrations of male and female genitalia are provided.
47.
2015.04 구독 인증기관·개인회원 무료
The Sphingidae are a family of moths (Lepidoptera, Bombycoidae), commonly known as hawk moths. The hawk moths form a family of over 1500 species of small to very large moths (40-150mm) occurring on all continents except Antarctica. The most conspicuous aspect of sphingid biology is the capacity of the moths for fast, long distance, and often migratory flight. Sphingidae adult are appropriately shaped with narrow wings and a fusiform abdomen. Sphingidae is very popular group with both amateur and professional naturalists, but the taxonomic study of the Sphingidae in Cambodia has been done by a few foreign entomologists. In the present study, we were collected 12 times from Cambodia in 10 area for 6 province. As the results of this study, about 455 species of 289 genera of 18 families belong to Lepidoptera (NIBR, 2009-2014). Of which, about 60 species of 30 genera of family Sphingidae were identified from Cambodia. Most of them are recorded for the first time in Cambodia.
48.
2015.04 구독 인증기관·개인회원 무료
농업체계에서 절지동물군집의 개체수와 종 다양성은 절지동물 그룹, 작물체계, 식물 밀도, 농약 사용과 관리 등에 따라 다르게 나타난다. 지표면에 서식하며 잡식성의 포식자로 알려진 딱정벌레목의 곤충은 함정트랩(pitfall)을 이용한 채집이 비교적 용이하여 생태계 환경 변화의 생물 지표로 많이 이용되고 있다. 유기농 과원의 딱정벌레목 곤충의 다양성 평가를 위해 경북 지역 5개의 유기농 과원을 선정하고, 각 과원 인근의 관행 재배 과원을 대조구로 삼았다. 딱정벌레목은 함정트랩을 이용하여 3년간 봄철 3회 반복 조사하였다. 전체 채집된 딱정벌레목은 12과 50종 1,181개체로 유기농 과원이 9과 41종 665개체, 관행 과원은 10과 33종 516개체가 조사되었다. 유기농과 관행 사과원의 딱정벌레목의 주요 그룹은 딱정벌레과(Carabidae)가 84%로 가장 높은 개체수 비율을 보였으며 점박이먼지벌레, 우수리둥글먼지벌레가 우점하는 것으로 나타났다. 두 관리 체계 간 평균 종수와 풍부도는 유기농 과원이 관행 과원보다 높은 결과를 보였으며 년도에 따른 차이도 나타났다. 종 다양도는 유기농 과원이 2.81, 관행 과원은 2.67로 유기농 과원이 높은 결과를 보였으며 균등도는 두 관리 체계 모두 0.76으로 비슷한 결과를 나타냈다.
49.
2015.04 구독 인증기관·개인회원 무료
The subgenus Nippocallis (Matsumura, 1917) is one of the 10 subgenera of the genus Tuberculatus Mordvilko, 1894. Originally, this subgenus was regarded as an independent genus of the subtribe Myzocallidina by the distinct morphological features and the host plant association with Castanea (Fagaceae). We performed molecular and morphological analyses to determine whether genus Nippocallis belongs to Tuberculatus group. Molecular analysis of the combined data set of Cytochrome Oxidase subunit I COI, Cytochrome B CytB and Elongation Factor I-alpha EF1α indicated that Nippocallis form a sister clade of the genus Myzocallis Passerini, 1860, apart from the genus Tuberculatus. In this contexts, we propose Nippocallis as a separate genus of the subtribe Myzocallidina.
50.
2015.04 구독 인증기관·개인회원 무료
The Chrysomelid species of the genus Xanthogaleruca, X. aenescens Fairmaire, 1878 is reportd for the first time from South Korea. The genus Xanthogaleruca Laboissiére, 1934, within subfamily Galerucinae, contains only nine species, and is distributed Palearctic region (Beenen, 2010), but one adventive species, X. luteola (Müller, 1766), is widespread throughout much of Canada, North Africa and the United States. Also known as the elm leaf beetle, it feed on Ulmus (Ulmaceae). In the present study we report on the genus Xanthogaleruca Laboissiére, 1934 from Korea for the first time, based on X. aenescens Fairmaire, 1878. We provide the habitus photographs, illustrations of diagnostic features and a key to the genus in S. Korea.
51.
2015.04 구독 인증기관·개인회원 무료
The brown soft scales, Coccus hesperidum (Linnaeus, 1758) is one of the most widely distributed species in the family Coccidae. Interestingly, this species shows considerable morphological variation depending upon the geography and host plants. Although, Korean populations also exhibit morphological variation between geographical regions, this species have been regarded as one species without intensive studies. In this study, we performed Neighbor joining analysis of combined data set of Cytochrome oxidase subunit I COI, 18S ribosomal RNA 18S rRNA, 28S ribosomal RNA 28S rRNA and Elongation factor I-alpha EFI-α genes for Korean populations of brown soft scales. The result of molecular analysis indicated that there are three distinct populations in Korea. Among three populations, one population is separated from two populations with 9% of molecular divergence. Based on high level of molecular divergence, we propose a cryptic species of C. hesperidum with description and illustrations of morphology.
52.
2015.04 구독 인증기관·개인회원 무료
Aedes albopicuts, known as asian tiger mosquito or forest mosquito, is characterized by its black and white strips on legs and body. Ae. albopictus is an important vector mosquito for the transmission of many viral pathogens such as dengue fever and chikungunya fever. Unlike other mosquito, Ae. albopictus attacks people mainly during the daytime in the forest. To evaluate the diel activity of Ae. albopictus, three times mosquito collecting were conducted using BG Sentinel trap with dry ice in bamboo forest in Damyang-gun, Jeoullanam-do, in South Korea from August to September, 2009. Captured mosquitoes were counted and released every one hour during 25 hours experiment time. The result showed that Ae. albopictus activity began with sun rising at approximately 09:00, with peak in the early evening between 16:00 to 19:00, and ended with sunset at 21:00. Among the several factors affecting on the mosquito activity, light intensity seems to be a main factor. Especially, if the light intensity is over some threshold, it negatively effects on the activity of Ae. albopictus.
53.
2015.04 구독 인증기관·개인회원 무료
Psyllids are a serious pests of pears cultivated in temperate and subtropical regions. Pear psyllids are a member of the large genus Cacopsylla (Psyllidae: Psyllinae). Among the 28 psyllid species that infect pear trees, Cacopsylla chinensis (Yang and Li, 1981) is considered the most harmful. Recently, we found new pear psyllid pest affected Korean pear (Pyrus pyrifolia var. culta) orchards. The psyllid is morphologically identical to C. chinensis and is herein reported as a new record. In this study, we conducted DNA sequence analysis of the mitochondrial cytochrome oxidase I-leucine tRNA-cytochrome oxidase II (COI-tRNALeu-COII) and 16S rDNA regions to demonstrate the phylogenetic relationships among C. chinensis from pear orchards in Korea, and those recorded from China, Taiwan and Japan. The sequence of the COI-tRNALeu-COII and 16s rDNA regions were equivalent from Korea. Comparison of nucleotide sequences and phylogenetic analysis differentiated Korean psyllids from the Chinese and Taiwanese C. chinensis, but Korean and Japanese psyllids were closely related. The results suggest that Korean and Japanese C. chinensis are genetically homologous. It is presumable that these individuals descended from a single colony that was probably introduced recently.
54.
2015.04 구독 인증기관·개인회원 무료
The genus Eurystylus Stål (Hemiptera: Heteroptera: Miridae: Mirinae) comprises to date 32 species in the world. This group is generally known as phytophagy, and some of the species are known as major insect pests of crops in various regions such as E. oldi Poppius, 1912, reducing grain mass and quality of sorghum crops by feeding and by ovipositing in Africa. Although these species are easily found and widely distributed, there are few taxonomic studies on this group in detail. In Korea, 2 species are known so far: E. coelestialium (Kirkaldy, 1902), E. luteus Hsiao, 1941. In this talk, the genus Eurystylus from the Korean Peninsula is reviewed for the first time. Biological information such as additional host plants of each species, and morphological characters of Eurystylus sp. nov. with other congeners from Korea are presented. Photographs of the Korean specimens, illustrations of the male genitalia, and diagnoses based on main characters with a key to the Korean species are also provided.
55.
2015.04 구독 인증기관·개인회원 무료
The longhorn beetle (Coleoptera: Cucujiformia: Chrysomeloidea: Cerambycidae) is one of the major family of coleoptera which contains more than 30,000 species worldwide. Because of their species-diversity, morphological attraction and ecological status, the family has received a massive global attention. However, in Korea, no thorough study on this family has been conducted within last twenty eight years. Here we update the latest knowledge of Korean longhorn beetle fauna. First, we give an historical overview of the longhorn beetle studies in Korea and correct some common species misidentifications. Then we provide the information of updated cerambycidae fauna with 4 new species and 9 species newly reported to Korea. Lastly, we discuss the importance of studying immature stage of cerambycidae and provide brief morphological, ecological information of two recently described species.
56.
2015.04 구독 인증기관·개인회원 무료
많은 학자들이 편리한 동정을 위해 검색키를 사용해 왔다. 현재 사용되고 있는 검색키로는 전통적인 검색키와 대화형 검색키가 있다. 전통적인 검색키는 기본적으로 트리구조로, 두 가지 형질 중에 양자택일을 하여 순차적으로 형질을 선택해 가는 방법이다. 기존에 주로 사용되어 왔지만, 양자택일의 방식을 사용하기 때문에 형질선택에 있어서 제한을 받는 반면에, 대화형 검색키는 트리구조와 관계없이 어떠한 단계의 형질이든 바로 이용할 수 있는 장점이 있다. 이 대화형 검색키 구조를 응용하여, Pictorial Key를 추가한 이미지형 동정시스템을 개발하였다. 이미지형 동정시스템을 이용할 경우에, 복잡한 용어나 수식이 필요 없이, 이미지만을 이용한 직관적인 동정이 가능하기 때문에, 전문가가 아닌 비전문가나 일반인들도 사용할 수 있는 검색키 작성이 가능해진다. 이 방법을 통해서, 본 연구에서는 한국산 나비 200종에 대한 이미지형 동정시스템을 구축하여, 일반인들이 사용하기 편리한 동정시스템을 구축하였다.
57.
2015.04 구독 인증기관·개인회원 무료
The genus Ypsolopha Latreille 1796, belongs to the family Ypsolophidae, small to medium sized micro-moth, with more than 140 described species around the world. More than 70% of the species are described from the Palearctic and Oriental Regions (Jin et al., 2013). Major taxonomic study of Ypsolopha carried out by following scholars. In the end of 18th to early of 20th century, Fabricius (1798), Hübner (1826), Agassiz (1846), Walshingham (1881, 1889), Busck(1903, 1906) and Meyrick (1914-1937). Furthermore, Moriuti (1964, 1977) studied total 17 Japanese Ypsolopha species and he divided 3 groups which based on fore- and hind- wing venations and shape of labial palpus. The first systematic study of the genus Ypsolopha carried out by K. T. Park (1983), discovered with four species, Ypsolopha blandella (Christoph), Y. yasudai Moriuti, Y. strigosa (Butler), Y. acuminata (Butler) in ‘Illustrated Flora & Fauna of Korea vol. 27 Insecta (Ⅸ)’ and ‘Check List of Insects from Korea’. After than, Ypsolopha longa Moriuti was discovered by Jeong et al.(1995). In 2001, Byun and Park, they described new species, Ypsolopha nigrimaculata Byun and Park. At the same time, Byun and Bae reported 3 newly recorded species; Y. cristata Moriuti, Y. japonica Moriuti, Y. parenthesella (Linnaeus). Up to date, 14 species have been recorded from Korea by Sohn (2010, 2011) with Y. amoenella (Christoph), Y. asperella (Linnaeus), Y. contractella (Caradja) and Y. acerella (Ponomarenko and Sohn). In the present study, we identified 14 species belonging to the genus, with some unidentified species. The materials examined come from the collection of Incheon National University.
58.
2015.04 구독 인증기관·개인회원 무료
The red-spotted apollo butterfly, Parnassius bremeri, immatures grow during winter and spring. Supercooling point of larvae during January goes much below -20℃. Morphologically, the larvae appear to be adapted to cold temperatures. Dark-colored body surface is useful to absorb solar energy and spiny integument may prevent any external ice formation on the body surface. Biochemically, P. bremeri larvae elevate glycerol as a cryoprotectant. This study reports two genes associated with glycerol biosynthesis in P. bremeri. Larval transcripts were analyzed using RNA-Seq technique. A total of 14 Gb transcripts were read by Illumina HiSeq and assembled to be 127,279 contigs. To specify the the genes associated with glycerol biosynthesis, a biosynthetic pathway to synthesize glycerol from dihydroxyacetone-3-phosphate was predicted with two genes of glycerol-3-phosphate dehydrogenase (GPDH) and glycerol kinase (GK). Both genes were annotated in the transcriptome of P. bremeri. Pb-GPDH encodes 166 amino acid residues containing NAD+-binding region, catalytic site, and calcium binding region. The predicted amino acid sequence was clustered with other lepidpopteran GPDH genes. Three Pb-GK genes were annotated from the transcriptome. Pb-GK1 encodes a full open reading frame of 514 amino acid residues. A ohylogenetic analysis showed that these three GKs were separately clustered. Interestingly, Pb-GK1 was clustered with other GKs that were known to be associated with rapid cold hardiness.
59.
2015.04 구독 인증기관·개인회원 무료
Naratettix Matsumura, 1931 (Hemiptera: Cicadellidae: Typhlocybinae) have been recorded 3 species such as Naratettix zini, N. koreanus, and N. rubrovittatus in Korea. In this study, Naratettix ibukianus Matsumura, 1931 is recognized as new to Korea. The male specimes is redescribed and illustrated and a key to the species of Naratettix is provided for the Korean species.
60.
2015.04 구독 인증기관·개인회원 무료
Insect immunity is innate and consists of cellular and humoral immune responses. Cellular immune response usually requires hemocyte-spreading behavior, which is accompanied by cytoskeletal rearrangement. A glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), catalyzes an oxidation reaction of glyceraldehyde-3-phosphate to 1,3-biphosphoglycerate in the cytosol. Another function of GAPDH in mammalian cell is to bind C-terminal α-tubulin to facilitate cytoskeletal arrangement. An immunoprecipitation (IP) of viral protein, CpBV-CrV1, against hemocyte protein lysate revealed that CpBV-CrV1 binds to GAPDH, identified by MALDI-TOF analysis. RNA interference (RNAi) of GAPDH significantly suppressed cellular immune response, but neither RNAi of hexokinase nor aldolase suppressed the cellular immune response. A common molecular motif of CpBV-CrV1 and a-tubulin at C-terminal region supported the IP analysis. To test the role of α-tubulin motif in CpBV-CrV1, point mutations of CpBV-CrV1 were applied and resulted in loss of the biological activity of CpBV-CrV1. Furthermore, an immunofluorescence assay indicates CpBV-CrV1 colocalized with a-tubulin in hemocytes collected from Plutella xylostella parasitized by Cotesia plutellae possessing C. plutellae bracovirus (CpBV). This result suggests that GAPDH plays a critical role in hemocyte-spreading behavior during immune challenge, and it is a molecular target of the pathogenic virus.
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