아세틸콜린에스테라제(ace)는 신경연접에서 흥분성 신경전달물질인 아세틸 콜론을 후신경연접으로부터 분해시킴으로서 곤충체내의 신경전달을 원활하게 해주는 기능을 하는 신경전달물질 분해효소이다. 이 효소는 해충방제라는 농 업분야에 있어서 유기인계와 카바메이트계 살충제의 목표물로 잘 알려져 있 으며, 해충방제를 목적으로 사용한 살충제들의 남용으로 인한 약제 저항성 문 제로도 잘 알려져 있다. 이 효소는 의학분야에 있어서도 아주 많은 연구의 대 상이다. 이 효소와 관련된 인간의 질병은 너무나 잘 알려진 치매와 연관이 있 다. 현재까지 치매의 종류는 몇 가지로 구분되어 지지만, 특히 많이 알려져 있는 것이 알쯔하이머형 치매이다. 이 알쯔하이머형 치매에는 현재 까지 3가 지의 가설이 있으며, 활발하게 그 원인과 발병기작 연구가 전 세계적으로 진 행 중이다. 3가지의 가설 중에서 ace와 관련된 가설이 cholinergic 가설이다. 이 cholinergic 가설의 주요 내용은 저농도의 살충제에 인간이 오랜 기간 동안 노 출이 되었을 경우에 생체내에서 조금씩 감소된 ace의 량이 되돌림 현상으로 작용되어 신경연접내에 정상보다 많은 ace가 발현되고, 그로 인해서 신경전달 물질이 정상보다 빨리 분해되어 신호전달에 문제가 발생됨으로 인해서 인지 능력저하와 기억력 저하와 같은 치매현상이 나타나게 된다는 내용이다. 그래 서 본 연구는 이와 같은 가설을 증명하기위해 초파리를 이용하여 ace를 과발 현 할 수 있는 형질전환 초파리를 제작하였고, 과발현시 나타나는 이상현상을 근신경연접에서 관찰 하였다.

This study was conducted to investigate the vegetable eating behaviors and preferences of elementary school students by stage of change for vegetable intake. The subjects consisted of 191 fourth and fifth grade elementary school students from Gyeonggi province. The stage of change for vegetable intake was categorized into three groups: precontemplation (PC, 18.4%), preparation (P, 43.5%), and action/maintenance (A&M, 48.2%). The trend to eat a variety of foods was more distinctive as the students went from the PC to the A&M stage. The stage of change for vegetable intake was closely related with the kimchi-eating pattern of the students. The A&M group ate more vegetables than the PC group because they liked vegetables and/or wanted to prevent disease. The PC group had a lower tendency to follow recommendations for vegetable eating than the P and A&M groups. The PC group also had an incorrect notion that low vegetable consumption over an extended time would not have a big impact on their health. The vegetables of high preference for the elementary school students were potato, sweet potato, lettuce, radish, perilla leaf, cucumber, and cabbage. The vegetables of low preference were spinach, onion, balloon flower, and green pepper. The PC group had lower preferences for most vegetables than the P and A&M groups. Therefore, careful nutrition education is necessary especially for students in the PC group in order to explain the positive effects of vegetable intake and negative effects of an unbalanced diet. It is also necessary to survey students' tastes consistently and to develop recipes that encourage them to consume more vegetables agreeably.

This study was conducted to investigate the distribution pattern, ecological characteristics and life cycle of the Black Soldier Fly (Hermetia illucens, BSF). The BSF was widely distributed throughout Korea. The insect was mainly found in the vicinity of and in cattle sheds, manure sheds, living waste dump grounds, and food waste dump grounds. Developmental characteristics of the BSF are as follows: the egg was long oval shaped of 887 ㎛ in the major axis and 190 ㎛ in the minor axis, it weighed 24 ㎍. Female oviposited ca. 1,000 eggs on average, eggs hatched in 81 hours under laboratory condition (27℃, 60% R.H.). The duration of the larval stage was approximately 15~20 days. The size of the last instar larvae was 21 ㎜. The cuticle of the pupae gradually acquired red-brown color and the size of them was 19 ㎜. The pupal stage was shorter for females (16 days) than males (15 days). Adults were sized about 13~20 ㎜ long and black-colored. The life span of adult insects was 5~8 days for the first generation (June~July), 7~10 days for the second generation (Aug.~Sept.), and 13~18 days for the third generation (Sept.~Oct.). Mating started on the next day of emergence and actively occurred at the third day after emergence. Mating mostly occurred between 10:00 and 16:00 during which light intensity is highest. Egg-laying started on the third day and was most frequent from the fourth to the sixth day after emergence. Similar to mating time, females oviposited mostly between 10:00 and 16:00.

친전자성 치환반응으로 제조된 술폰화 단량체, 비(非)술폰화 단량체 및 탄산칼륨을 이용하는 직접 중합법을 통하여 높은 점도의 술폰화 폴리아릴에테르술폰 공중합체를 합성하고, 이들을 원료로 N-메틸-2-피롤리돈(NMP)과 디메틸아세트아미드(DMAc)의 혼합 용매 상에서 직접 메탄을 연료전지용 고분자 전해질 막을 제조하였다. 막 제조 시의 용매 효과에 주목하여 혼합 용매의 부피 비는 0~100%로 변화시키고 공중합체의 술폰화도는 50%로 고정하였다. 이온 전도도 및 메탄올 투과도 측정을 통하여 최종 전해질 막의 기본 특성을 파악하고, 주사전자현미경 및 원자간력현미경분석을 통한 표면 분석 결과와 비교함으로써 이들의 상관관계를 고찰하였다. 막 제조 시의 용매 혼합 비율을 적절히 조절함에 따라 최종 전해질 막의 이온 전도도를 크게 향상시킬 수 있음이 확인되었는데, 25℃의 100% 가습 환경에서 측정된 수소 이온 전도도는 NMP : DMAc 50:50 부피/부피-%에서 최대 1.38×10 -1 S/cm이었다.

This study aimed at providing fundamental data to take health care plan in the aspect of Oriental medicine by evaluating the lifestyle and yangseng(養生) levels by job-type of industrial workers. General characteristics, lifestyle, and yangseng levels were investigated through self-administration questionnaire in the course of Oriental health examination for 1,190 male workers at a manufacturing company located in the Chungcheongnam-do region, and the following results were obtained. The total mean of yangseng levels was 96.73 and the evaluation mark was 3.12. The highest mark was shown in the morality yasngseng, while the lowest mark in the activity and rest yangseng. In general, the higher were age and income level, the higher were yangseng levels, and yangseng levels were found to be high in non-smokers and those who have regularly exercised. For yangseng levels by job-type, total yangseng levels were found to be high in office workers, engineer workers, and manufacturing workers in order. For detailed categories of job-type including diet yangseng, activity and rest yangseng, sleeping yangseng, seasonal yangseng, and sexual life yasngseng, yangseng levels were generally found to be high in office workers but low in manufacturing workers. In this study, difference in yangseng levels could be found by job-type of industrial workers, and lifestyle and general characteristics were found to influence yangseng levels. Therefore it is deemed that close studies on health care by job-type are required for health promotion and care of industrial workers in the future.

This study was carried out to analyze any possible changes in the heart rate variability(HRV) of white-collar workers of a manufacturing firm based in Chollabuk-do Province when they are stressed due to their daily job performance and thereby to help them seek a more effective method of the management of their health and help them form more desirable habits of living by finding out how seriously his or her stresses from his or her job performance affect their quality of life(QOL). As a process of the above-mentioned manufacturer's plan to do an Oriental health examination on its white-collar workers, each of the workers of the firm was given a copy of questionaires. The questionaires carried questions regarding each of the workers' general characteristics, clinical history, health-related lifestyle and the QOL and each of the workers was asked to provide his answers to the questions listed on the questionaire. The examination progressed to measure any possible changes in the HRV of an individual worker. Among all of the questionaires filled out and collected from the workers, a total of 423 data were used as the data of the research except those that had answers which were not appropriate for the purpose of the research. The analysis of each of the workers' health related quality of life(HRQOL) showed that the younger, the lower the standard of education is, each of the subjects was found to tend to show a relatively higher QOL if only he is healthy. With regard to the worker's habits of living, those who exercise on a regular basis and who get a good sleep were also found to have a comparatively higher QOL With reference to the HRV, young workers, those who have spouses, those with a high level of academic background, those with a short work experience were found to tend to have a relatively higher HRV. As regards each worker's habits of living, those who do exercise on a regular basis were found to have a comparatively higher index of HRV. RMSSD was found to have an impact on workers' general health, on the other hand TP was found to have an impact on workers' role of emotion. This result was based on findings through analyzing interrelation between HRV and QOL that stresses have an impact on the QOL. Taking a look at the above results, it was learned that individual worker's efforts to promote his or her health, along with establish desirable habits of living, these two qualities are important methods to improve the quality of life. In addition, control of stresses is necessary to improve the QOL, which is possible through emotional stabilization.

최근 전자태그(RFID : Radio Frequency Identification)를 부착하여 비접촉식으로 다 양한 물품의 정보를 받아 물품의 추적 및 정보확인이 가능한 RFID 시스템을 작업현장에 도입/확산시키고자하는 연구들이 진행되어지고 있다. RFID 시스템은 무선인터페이스 환경에서 태그는 안테나에 정보를 담은 주파수를 보내고 있으며, 이 환경에서의 작업자는 주파수에 노출되어있다. 따라서 작업자 주파수에 노출되는 RFID 시스템 환경 에서 주파수가 작업자에게 미치는 영향에 대한 안전성평가가 필요하다.

The purpose of this study was to investigate whether or not ‘focus on form’ instructions can be applied to 7 year old children"s communication classes and how many uptakes are observed from each class. And if it works, what types of ‘focus on form’ will be observed and which types of ‘focus on form’ are most useful for learners" uptakes. This study observed 9 different kinds of ‘focus on form’ types and divided into two higher types: Reactive Focus on Form and Preemptive Focus on Form. From this study, there were 103 ‘focus on form’ episodes which were recorded in order to identify occasions where there was attention to linguistic form. After 98 times, learners’ uptakes were examined from 103 ‘focus on form’ episodes. Learner uptake was generally higher and successful, to a much greater extent than has been reported for ESL adult classrooms. Uptake was higher in ‘reactive focus on form’ than in ‘preemptive focus on form’, in teacher-initiated ‘focus on form’ than in student-initiated ‘focus on form’, and also higher when blended with reactive and preemptive focus on form. Having accomplished this research I conclude it is necessary when attempting to examine young learner uptakes during ‘focus on form’ instructions in a communication class, the teacher should plan to use the most effective types of focus on form ‘recast’ and teacher-initiated episodes as possible, rather than student-initiated episodes.

푸른 곰팡이병을 일으키는 병원균은 매우 다양하며, 현 재까지 밝혀진 병원균은 Trichoderma spp., Gliocladium spp., Penicillium spp. 그리고 Aspergillus spp.로 이 중에서 Trichoderma spp.는 자낭균류에 속하는 Hypocrea spp.의 무성세대로서 발생빈도가 가장 높은 것으로 알려져 있다. 버섯의 병해 중에서 가장 방제가 어렵고 버섯의 발생 및 수확에 가장 큰 영향을 미치는 대표적인 병의 하나인 푸른 곰팡이병은 고온다습한 조건에서 많이 발생하며 발생초 기에는 버섯균과 비슷한 백색을 띄다가 포자가 형성되면 서 황록색 내지 진녹색으로 변하고, 골목의 표면에 부정형 의 병반을 형성하면서 버섯균의 생장을 억제하여 결국에 는 버섯균을 죽게 한다. 현재 버섯 재배농가에서는 버섯균 사생장 기간동안에 푸른 곰팡이병의 발생을 억제하기 위 하여 버섯배지 살균 전에 균상표면에 벤레이트, 판마쉬, 스포르곤수화제를 살포하는 것이 최대의 방제법이다. 따 라서 본 연구는 버섯 병해의 주범인 그린몰드(green mold)의 생장을 제어하기 위하여 수목추출물 및 nhexane 용해부의 효능을 비교 평가하였다. 수목추출물의 그린몰드에 대한 항균활성은 T. aggressivum 34.7%, T. atroviride 35.7%, T. harzianum 34.9%, T. koningii 31.0%, T. viride 30.7%로, 평균 33.4%의 항균 활성을 나타내었고, T. atroviride에서 수목추출물이 가장 높은 항균활성을 나타내었다. n-Hexane 용해부의 그린몰드에 대한 항균활성은 T. aggressivum 52.3%, T. atroviride 65.6%, T. harzianum 47.1%, T. koningii 48.5%, T. viride 51.1%로, 평균 52.9%의 항균활성을 나타내었고, T. atroviride에서 nhexane 용해부가 가장 높은 항균활성을 나타내었다. 수목추출물과 n-hexane 용해부의 그린몰드에 대한 항 균활성은 추출물의 농도에 따라서 5% 수준에서 유의성이 나타났다. 수목추출물과 n-hexane 용해부의 항균활성을 비교하 였을 때 수목추출물보다 수목 n-hexane 용해부에서 더 높은 항균활성이 나타났다.

표고, 느타리, 양송이, 팽이등 버섯의 종류에 관계없이 발생되고 있는 푸른곰팡이병은 그린몰드(green mold)가 그원인으로 알려져 있으며 이들의 피해는 심각하다. 푸른 곰팡이병을 일으키는 곰팡이로는 Trichoderma, Gliocladium, Aspergillus, Penicillum 등이 현재 알려져 있다. 푸른곰팡 이 병원균은 균사생장 시 균상에 발생하여 버섯균의 균사 생장을 방해하거나 기생하여 버섯균사를 사멸시키며, 버 섯수확기에 병해를 발생시키고 있다. 따라서 본 연구는 버 섯의 수확을 저하시키는 Trichoderma를 억제하기 위하 여 수목추출물 2종을 혼합한 다음 그 효능을 평가하였다. Trichoderma에 대하여 항균력을 나타내는 유효 화합 물질을 확인하기 위하여 GC/MS를 이용하여 편백 휘발성 추출물을 분석한 결과, 50개의 테르펜계 화합물이 존재한 다는 것을 확인하였고 대부분의 물질이 동정되었다. 편백 휘발성 추출물의 주요 화학적 조성은 모노테르펜류인 sabinene 11.62%, limonene 4.31%, isobornyl acetate 6.23%, α-terpinyl acetate 11.75%였으며, 세스퀴테르 펜류로 elemol 11.08%, β-eudesmol 4.02%, epibicyclosesquiphellandrene 4.18%가 나타났다. 소나무와 편백 추출물의 혼합비율 10 : 1(g/g) 제제는 T. aggressivum에서 20.4%, T. atroviride에서 14.0%, T. harzianum에서 32.0%, T. koningii에서 26.8%, T. viride에서 19.0%의 항균활성을 나타냈다. Trichoderma 균주는 편백 추출물의 첨가량이 증가함에 따라 항균활성 이 감소하는 경향을 나타냈다. Trichoderma 균주는 편백 추출물보다는 소나무 열수추출물에 대하여 영향을 받았다.

In this study, the apoptotic effects of the actin disruption agent, latrunculin B(LB) have been investigated on p53 deficient chronic myeloid leukemia cell line K562. A dose-dependent decrease in K562 cell proliferation was observed after LB treatment with maximum decrease in cell proliferation being at 1.5μM where the percent inhibition was 66.53%. F-actin stained with TRITC-phalloidin was shown as a peripheral ring or appeared diffusely distributed throughout the cytoplasm in untreated cells, this actin ring was decreased following LB treatment, and even large focal actin aggregates were formed. Treatment of K562 with LB(1.5μM) generated ROS substantially. LB activated expression in a dose-dependent manner. Therefore it can be concluded that LB, depolymerising agent of actin, induces apoptosis by producing ROS and up-regulating NF-kB and COX-2 activation.

본 연구는 개구부를 통해 음향적으로 결합된 공간에서의 잔향특성변화를 축소모형을 이용하여 살펴보았다. 대상공간의 음향적 결합은 정방형의 개구부를 통하여 이루어졌으며, 개구부의 크기, 부실의 흡음력 및 위치를 변화시켜가며 실의 음향특성변화를 측정 분석하였다. 연구결과 동일한 체적을 가진 부실을 통해 주실의 음향특성을 조절하는 경우 부실의 흡음력을 주실보다 현저하게 낮게 하는 것이 넓은 범위의 음향조절이 가능하며, 음향특성을 단계적으로 조절하고자 하는 경우 주실보다 높은 흡음력을 가진 부실을 이용하는 것이 효과적인 것으로 나타났다. 잔향부실의 경우 음원 측에 위치시키는 것이 효과적이며, 개구율이 6.25%이상일 때부터 주실의 음장특성에 중요한 영향을 미치는 것으로 분석되었다. 그러나 개구율이 25%이상으로 증가되면 개구율 증가에 따른 주실의 유의적인 음향특성변화는 더 이상 나타나지 않았다. 흡음부실이 결합되는 경우 음원으로부터 멀리 위치시키는 것이 효과적이었으며, 개구율 3.13%이상일 때부터 주실의 음장특성변화가 가능하였다.