본 논문은 Keller의 ARCS 모델을 기반으로 하는 유아 대상의 수학교육 CAI 프로그램 설계를 제안한다. 이를 위해 본 논문은 NCTM의 다섯 가지 영역 중 수와 연산 영역, 기하 영역, 자료 분석과 확률 영역에서 색, 수 세기, 도형, 방향을 포함한 수학 교육 CAI 프로그램을 개발하였다. 수학 교육 CAI 프로그램은 유아가 직접 카메라를 사용하여 RGB를 이용한 색 인식, 윤곽선 근사화와 허프변환을 이용한 도형 인식, 색 위치 기반의 방향 인식에 대한 미션을 수행하면서 학습하게 해 게임 몰입을 유도한다. 또한 동물형 캐릭터와 캐릭터가 제시하는 미션을 수행하게 함으로써 유아의 흥미를 유발하여 주의를 집중시키고 유아 스스로 학습과 생각할 수 있게 해 학습을 하면서 미션을 성공이나 실패시에 캐릭터의 칭찬과 격려를 통해 자기 효능감을 높이고 자신감을 가질 수 있는 환경을 제공한다.

목적: 본 연구는 안경수건에 함유된 은나노 입자의 항균효과를 연구하기 위해 수행되었다. 방법: 은나노 입자가 함유된 안경수건과 함유되지 않은 안경수건의 세균수를 비교하였다. 결과: 은나노입자가 함유된 안경수건에서 뚜렷한 세균수의 감소가 관찰되었으며, 이 결과는 안경렌즈에 존재하는 세균에 대해 은나노 입자가 높은 항균성을 가짐을 의미한다. 결론: 가까운 미래에 나노기술을 이용한 항균작용 안경수건의 개발이 기대된다.

본 연구는 돼지 B-casein 유전자 위치에서 EGFP가 발현될 수 있는 knock-in 벡터를 구축하기 위하여 실시되었다. 돼지의 B-casein 유전자를 이용하여 knock-in 벡터를 구축하기 위해 돼지의 태아 섬유아세포로부터 B-casein 유전자를 동정하였고 EGFP, SV4O polyA signal을 동정하였다. Knock-in 벡터는 5' 상동 영역 약 5 kb와 3' 상동 영역 약 2.7 kb로 구성되어있으며, positive selection marker로 neor 유전자를, negative selection marker로 DT-A 유전자를 사용하였다. 구축된 knock-in 벡터로부터 EGFP의 발현을 확인하기 위하여 생쥐 유선 세포인 HC11 세포에 knock-in 벡터를 도입하였다. 그 결과 EGFP의 발현을 HC11 세포에서 확인하였다. 이와 같은 결과로서 이 block-in 벡터는 knock-in 형질전환 돼지를 생산하는데 사용될 수 있을 것으로 생각된다.

고흥지역 일대에 분포하는 백악기 화강암을 대상으로 석영내에 발달해 있는 아문미세균열을 측정하여 연구지역의 고응력장을 분석하였다. 5개의 시료(GH-1, GH-3, GH-4, GH-5, GH-8)에서 아문미세균열들의 방향을 측정하여 분석한 결과, GH-1, GH-3, GH-4에서 최대수평주응력은 N60˚W와 N70˚E, N20˚W, N50˚W의 방향성이 가장 우세하였으며, N-S와 N30˚E의 방향성도 미약하게 나타났다. GH-5와 GH-8시료에서는 최대수평주응력의 방향 N40˚E와 N10˚E가 가장 우세하였고, N40˚W의 방향성도 미약하게 나타났다 전체적인 최대수평주응력의 방향은 N60˚W가 가장 우세하였으며, N20˚W, N20˚E, N70˚E등의 방향성도 미약하게 나타났다. 이 연구의 결과와 기존의 고등력장에 관한 연구결과를 종합해 볼 때, 백악기 말에서 신생대 3기 초 사이 연구지역에 작용한 최대수평주응력의 방향은 WNW에서 NE로 변화했을 것으로 판단되며, 그 원인으로는 그 당시 동북아시아 일대에 일어났던 복잡한 지구조운동에 기인한 것으로 사료된다.

본 연구는 사람 H-transferase가 과발현하는 돼지 체세포주를 개발하는데 있다. 돼지 세포에 사람 H-transferase 유전자를 발현시키는 것은 이종간 장기 이식에 있어서 초급성 거부 반응을 방지하기 위한 한 가지 방법이다. 본 연구에서는 과발현 벡터를 구축하기 위하여 사람 H-transferase을 HepG2 세포로부터 동정하였으며, 이 유전자를 CMV promoter를 이용하여 발현할 수 있도록 포유동물 발현 벡터인 pRc/CMV 벡터에 삽입하였다. 또한, 돼지 산자의 귀 세포를 이용하여 체세포를 수립한 후 jetPEI DNA transfection reagent를 이용하여 벡터를 도입하였고, 300 μg/ml의 G418로 12일간 선별하였다. PCR을 이용하여 선별된 colony들을 분석한 결과, 벡터가 도입되었음을 확인하였고, RT-PCR을 이용하여 사람 H-transferase mRNA가 발현하는 것을 확인하였다. 본 연구에서 확립된 세포주는 사람 H-transferase가 과발현하는 형질 전환 돼지의 생산에 이용될 수 있을 것으로 생각된다.

체세포의 배양 방법은 체세포 핵이식에 의한 형질 전환 돼지 생산에 있어서 중요한 요인 중 하나이다. 본 연구에서는 돼지 태아 섬유 아세포의 효율적인 배양 방법을 수려하였다. 돼지 태아 섬유 아세포는 임신 33일째 태아로부터 제조하였으며, 돼지 태아 섬유아세포의 증식을 혈청과 배지 종류별로 분석하였다. 그 결과, 15% ES screened FBS가 포함된 DMEM 배지에서의 배양은 15% FBS보다 세포수의 증가가 훨씬 더 빠르게 나타났다. 또한, 태아

In this study, we improved the water-based condensation particle counter in Atmospheric Research Aircraft NARA and investigated the condensation particle number concentration over the Korean peninsula. Pump and set point information were changed to improve the instrument used by aircraft for observation. Ground-based observational result showed that the error between two instruments, which are water-based condensation particle counter and butanol-based condensation particle counter, was 4.7%. Aerial observational result revealed that the number concentration before improvement indicate large variation with unstable condition, whereas the number concentration after improvement indicate a reasonable variation. After improvement, the number concentration was 706±499 particle/㎤ in the West Sea and 257±80 particle/㎤ in Gangwon-do, and these are similar to the concentration range reported in previous studies. Notably, this is the first attempt to use aerial observation with water-based condensation particle counter to investigate condensation particle number concentration.

The purpose of this study is to examine the effectiveness of using songs for improving vocabulary acquisition and memory. A total of 19 students who are learning Korean as a second language participated in this study. They were divided into two groups; the experimental group who had their vocabulary class with the aid of a song and the control group who had the same class but without using the song. The pre-test results showed that within the two groups, there were no significant differences in the participants' knowledge of the given words. Each group had an immediate post-test right after their class. After a two-week delay of no class, they took another post-test and were given a questionnaire. It was hypothesized that there would be a significant difference in both short-term and long-term memory between the two groups. Looking at the results, there was a significant difference in the immediate post-tests between the groups. While the use of song in vocabulary teaching did not have a considerable impact on the short-term memory of the participants, there was a significant difference found between the groups in the delayed post-tests. This indicates that the use of song has a great influence on transferring a short-term memory into a long-term memory. These findings suggest that the use of a song in teaching Korean vocabulary may aid in the acquisition and memorization of vocabulary. These would make a great contribution to developing teaching materials and learning strategies for KSL learners.

As the pilot study on textile reinforced mortar(TRM) construction method, the present research has been conducted to investigate the tensile performance of fiber-reinforced polymer(FRP) grids inserted in inorganic mortar. For this purpose, experiment was carried out using twenty specimens with the main parameters of inorganic mortar and FRP grids. As a result, splitting failure did not occur between inorganic martar and FRP grids. As FRP grids were completely fractured, the specimens failed by showing the similar tensile behavior to the mechanical properties of FRP grids.

The object of this study is to investigate the confined effects of specimens using GFRP(glass fiber reinforced polymer) sheet and PET(polyethylene terephthalate) sheet. Since GFRP and PET sheet have a grate deformation capacity, the brittle failure of concrete can be avoid. A total of specimens is sixteen and major variables are the type of strengthen material and strengthening layer. As a result of test, specimens reinforced with a glass fiber sheet compare to the standard specimen have a significant increase both strength and ductility. Ductile of the specimens reinforced with PET is superior to it of the GFRP regardless of strengthening layer. However, in the strength effect aspect is not great compare to the ductile. Resultingly, it is judged that the reinforced method with PET is available to apply field application.

An ICH RC (internally confined hollow reinforced concrete) tower for supporting 5.0MW wind turbine was developed and its standard sections were designed. It was found that ICH RC wind tower is satisfied with maximum 200% bigger moment which is required for pre-existing steel tower. Also, the results from the FAST showed that 5.0MW turbine can be normally operated with the ICH RC tower.

A standard design section of a FRP DSCT wind power tower supporting 3MW was suggested and designed through AutoDSCT and CoWiTA programs. The thicknesses of the FRP tubes were optimized and by using the parameters of designed tower, the performance of the new type wind tower was evaluated via FAST program.

Background : This study aimed to investigate the antioxidant and antiproliferative activities of extract from different parts of water chestnut (Trapa japonica Flerow). Methods and Results : The total polyphenol content of pericarp and seed extract was 438.31 ㎎/g and 25.32 ㎎/g respectively. DPPH radical scavenging assay showed that the half maximal inhibitory concentration (IC50 values) of pericarp and seed extract were 5.28 ㎍/㎖ and 355.51 ㎍/㎖ respectively. In addition, the pericarp extract showed strong reducing power. In the MTT assay, the pericarp extract significantly inhibited the viability of A549, AGS, HeLa, PC-3, HCT116, HT29 and SW620 cell lines compared with the seed extract. Conclusions : These results suggest that T. japonica Flerow extracts have significant antioxidant and antiproliferative activity.

Transverse reinforcement in reinforced concrete column can improve strength and ductility by confining column to expend laterally. The effects of enhancement of strength and ductility depend on the degree of confinement on transverse reinforcement. The theoretical equations about confinement effects of reinforcement concrete column were proposed by earlier investigators. In this study, it was identify the factor for significant impact on reinforced concrete circle column based on early models, and Parameter studies was carried out by each factor. Parameter studies decide the major variables for vertical interval and yield strength of transverse reinforcement, compressive strength of concrete, and sectional area. In Parameter study results, it was identify that vertical interval of transverse reinforcement is significant impact on the strength and ductility.

There has been little research on the harvesting time-dependent changes in the antioxidant activities of new varieties of highland cultures of Gomchwi (Ligularia fischeri) crossed with Turcs (Ligularia fischeri (Ledeb.)) and Nakai (Ligularia fischeri var. spiciformis), namely Sammany (S), Gommany (G) and Damogy (D). This study was conducted to assess the effect of different harvesting times on nutritional and health-related properties such as total phenolic contents, flavonoids, DPPH (1,1-diphenyl-2-picrylhydrasyl) free radical scavenging activities and reducing power. From these harvests, extracts were prepared using methanol. Total phenolic content in Jul 14-G (Gommany harvested on July 14, 0.172 ㎎·GAE/㎖) was higher than that in other products harvested after the same period (S, 0.154; D, 0.141 ㎎·GAE/㎖). Flavonoid content was higher in Jul 3-G (0.114 ㎎·QE/㎖), compared to Jul 3-S (0.113 ㎎·QE/㎖) and Jul 14-D (0.089 ㎎·QE/㎖). Antioxidant activities were higher in samples harvested after June 12 in all cases. On July 14, the highest DPPH free radical scavenging activities among all harvest dates were seen (92.875~94.595%). The reducing power was also dependent on harvest day (Abs 0.5~0.6 on July 14), showing a pattern similar to that of DPPH free radical-scavenging activities. Antioxidant activity and harvesting times seem to correlate with total polyphenol and flavonoid contents.