A bacterial colony was isolated from the gut of the bean bug, Riptortus clavatus. From morphological and biochemical tests, the bacterial isolate showed the highest similarity to Staphylococcus succinus. DNA sequence of 16S rRNA gene of the bacterium supported the identification. Oral administration of penicillin G to adults of R. clavatus gave a dose-dependent mortality of adults of R. clavatus to adults along with significant decrease of the bacterial population in the gut. Similarly, three metabolites (benzylideneacetone, proline-tyrosine, and acetylated phenylalanine-glycine-valine) derived from an entomopathogenic bacterium, Xenorhabdus nematophila, also inhibited growth of the gut bacterial population and gave significant mortalities to R. clavatus. These results suggest that a gut bacterial population classified as Staphylococcus sp. is required for survival of R. clavatus and that the three bacterial metabolites had toxic effects on the bugs due to their antibacterial properties.

The bean bug, Riptortus pedestris (=clavatus) Fabricius (Hemiptera: Alydidae) is a serious pest of soybean in many countries. It enters a reproductive diapause in the winter by short day length (＜13.5 h.) in Korea and Japan. The combined effects of temperatures (25℃ and 8℃) and day lengths (10L:14D and 14L:10D) upon the termination of the male"s diapause were studied. The combinations are ① HTLD = 25℃, 14L:10D treatment for 1, 2, 3 weeks and 30 days, ② HTSD = 25℃, 10L:14D treatment for 1, 2, and 3 weeks, ③ LTLD→HTLD = 8℃, 14L:10D treatment for 1, 2, and 3 weeks followed by HTLD treatment, ④ LTSD→HTLD = 8℃, 10L:14D treatment for 1, 2, and 3 weeks followed by HTLD. The amount of aggregation pheromone components secreted was adopted as the criteria for diapause termination in males. Males did not secrete aggregation pheromone under HTSD conditions. However, the males treated with HTLD for more than 21 days secreted significantly higher amounts of aggregation components, (E)-2-hexenyl (Z)-3-hexenoate and (E)-2-hexenyl (E)-2-hexenoate, compared to the control or HTSD treatments. The three components of the aggregation pheromone were secreted by the 14%, 29%, and 100% males treated with HTLD for 7 days, respectively. However more than 83% of the HTLD-treated males secreted all three components by the 30<SUP>th</SUP> day under the treatment. In comparison, the pheromone amounts secreted by the males treated with LTLD→HTLD or LTSD→HTLD were equal or less than those secreted by males with continuous HTLD treatment.

The bean bug, Riptortus clavatus Thunberg (Hemiptera: Alydidae), is a pest of soybeans and tree fruits. It enters reproductive diapause during winter. We studied the effect of different combinations of temperature, day length, and treatment period on the termination of diapause in R. clavatus using adult females collected in October and November 2006. Ovarian development was used to determine diapause termination. The treatments were: ① HTLD, 25℃, 14L:10D treatment for 1, 2, 3 weeks and 30 days, ② HTSD, 25℃, 10L:14D treatment for 1, 2, and 3 weeks, ③ LTLD, 8℃, 14L:10D treatment for 1, 2, and 3 weeks followed by HTLD for 3 weeks, and ④ LTSD, 8℃, 10L:14D treatment for 1, 2, and 3 weeks followed by HTLD for 3 weeks. The HTSD treatments did not affect ovarian development, and resulted in no significant difference in the number of mature eggs in ovaries or the percentage of diapause-terminated females compared to the control females before treatment. The percentage of females that terminated diapause was significantly higher in the HTLD treatment than in the HTSD treatment. The HTLD treatment for more than 14 days increased the percentage of diapause-terminated females, accelerated the development of the ovaries, and increased the number of mature eggs in ovaries. Compared with the HTLD or HTSD treatments, the LTLD or LTSD treatments followed by the HTLD treatment accelerated ovarian development and increased the number of ovipositing females. The pre-LTSD treatment for 1 week was enough to increase the number of eggs oviposited.

산림지(밀양 화악산)와 농경지(밀양, 식량과학원 기능성작물부 내)에서 톱다리 개미허리노린재의 발생양상을 조사하였다. 집합페로몬을 이용한 톱다리개미허리 노린재의 발생은 봄철(4～6월)에는 농경지보다 산림지에서 약 3배 이상 많았으며, 여름철(7～9월)에는 산림지보다 콩 포장에서 약 2.5배 많았고, 콩 포장 침입 최성기는 8월 상순 이었다. 집합페로몬에 의한 시기별 일중(日中) 시간대별 톱다리개미허리노린재 암컷과 수컷의 발생비율은 8월 중순 및 하순 14:00～16:00에 각각 50～53% 및 38～39%로 가장 높았으며, 9월 하순에는 12:00～14:00에 36～41%로 가장 높았다. 불출법(Flushing method)에 의한 톱다리개미허리노린재의 9월 상순 시간대별 발생량은 14:00>16:00> 12:00>10:00>08:00>06:00순으로 많았다. 따라서 톱다리개미허리노린재는 봄철에 산림지에서 여름철에서 가을까지는 콩 등의 농경지에서 많이 발생되었으며, 노린재의 활동은 오전보다 오후에 활발하였고, 시간대별 발생비율은 낮의 길이에 따라 영향을 받았다.

Male adults of bean bug, Riptortus clavatus Thunberg (Heteroptera: Alydidae), release aggregation pheromone (AP) which consists of (E)-2-hexenyl (Z)-3-hexenoate (E2HZ3H), (E)-2-hexenyl (E)-2-hexenoate (E2HE2H), and tetradecyl isobutyrate (TI). Variation in the pheromonal secretion were checked by the factors of age, mating status, seasons, body weight and time in a day. There were no significant differences in the amounts of AP secretion by mating, body weight, and time in a day. Higher amount of AP was detected from older males than younger ones. The amount of AP detected was higher in the males collected from April to September than in those collected during October. No AP was detected in the males of November and December. These phenomena in the AP secretion were discussed in relation to food exploitation and reproductive diapause of the bean bug.

The kairomonal activity of the aggregation pheromones of Riptortus clavatus for the egg parasitoid, Gryon japonicum , were investigated in soybean field. The parasitism rate of G. japonicum gradually decreased when the distances between pheromone traps and egg sites were increased from 0 to 15 m. The active distance of pheromone source for parasitoid attraction was estimated to be ca. 15 m. Under field conditions, the parasitoid wasp population peaked during the late August and early September, and the parasitism rate increased when the number of pheromone traps was increased from one to three per 165 ㎡. However, insecticide-treated plots, the level of parasitism recorded is relatively low. Sex ratios in field populations of G. japonicum were female-biased.

This study was conducted to determine pheromone trap type and height in forecasting populations of R. clavatus in soybean fields using pheromone. The most effective pheromone trap type and height in forecasting populations of R. clavatus were fish trap and 60 ㎝ above ground. Ratio of R. clavatus adults female and male in soybean field was 1.5 to 1 and Piezodorus hybneri was also attracted to the aggregation pheromone trap of R. clavatus. Attractiveness of two stink bug species caught on synthesis pheromone of R. clavatus was surveyed with imported production and synthesized production. Imported pheromone attracted only adult of R. clavatus, but synthesized pheromone attracted both adult of R. clavatus and P. hybneri. Change of population of R. clavatus was observed using pheromone trap and sweeping method in soybean field. Adults of R. clavatus occurred from early August and the population reached its peak in early September when pheromone trap was used. In case of sweeping method, its fluctuation pattern was similar to that of pheromone trap.

Paromius exiguus (Distant) has caused serious damage by pecky grains around Gimpo paddy fields in 2001. We conducted field and laboratory studies to determine the seasonal occurrence and age distribution of P. exiguus on the three major host plants. The overwintering P. exiguus was found mainly on the basal part of gramineae weeds in various localities. After overwintering, in mid-May, the adults aggregated on the grain parts of Imperata cylindrica, laid their eggs and nymphs developed into adults on the same host plants. By the time, the Calamagrostis epigeios colony had newly occupied I. cylindrica areas, the nymphs and adults of first generation had already moved to the second host. The second generation of P. exiguus, after having completed its life cycle on C. epigeios, the newly emerged adults migrated to the rice plants and other gramineae weeds in early August. Afterwards, they complete its third generation cycle where they can move to the overwintering site again. P. exiguus has the five nymphal stages and each nymphal stage could be determined by head or prothoracic width. On the I. cylindrica and O. sativa hosts, the age distribution of P. exiguus showed a simple structure as each stage ratio increased stepwise with time. But in case of C. epigeios, as the newly emerged adults and immature nymphs continuously migrate after a month from the I. cylindrica, the age structure became remarkably complex. The peak nymphal density was observed when the ratio of third and forth instar was the highest in the population. The finding about the specific age structure on each generation of the insect would be very useful in control decision making on the major host plants. It is also important to consider the host"s specificity to pesticide sensitivity in relation to various nymphal stages.

Oviposition preference of the bean bug, Riptortus clavatus to sites on a plant and within a field, to plants at different developmental stages, and to different leguminous germplasms was observed. The insect layed its eggs mainly on the back surface of leaf in the upper half of plant in both observation from oviposition cage and soybean field. The egg number in fields were observed at a statistically-same rate in marginal and inside area, and at a median value of one egg per leaf in oviposited leaves. Full seed stage of soybean hardly affected oviposition preference of the insect. A statistically-significant difference in oviposition to different leguminous germplasms was observed.

콩의 주요 노린재류인 톱다리개미허리노린재와 가로줄노린재의 유인력을 증진시키기 위하여 유인물(집합페로몬과 콩 조합)과 4종류의 유인트랩을 조합하여 그 효과를 검토하였다. 톱다리개미허리노린재의 집합페로몬은 3성분(EZ:EE:MI)과 4성분(EZ:EE:MI:OI)의 2종류로 이들 집합페로몬의 조성성분에 따른 톱다리개미허리노린재의 유인효과는 3성분보다 4성분에서 약 35% 증진되었다. 집합페로몬을 청자콩, 검정콩 및 태광콩의 종실과 각각 조합하면 톱다리개미허리노린재의 유인효과는 4성분+청자콩>4성분>검정콩>4성분+태광콩>3성분+청자콩>3성분+검정콩>4성분>3성분+태광콩>3성분의 순으로 높았다. 유인트랩에 따른 톱다리개미허리노린재의 유인효과는 통발트랩>펀넬통기트랩>펀넬트랩>풍뎅이트랩 순으로 높았다. 가로줄노린재의 집합페로몬(EE) 함량에 따른 유인효과는 105㎎>80㎎>50㎎ 순으로 높았다. 집합페로몬과 콩 종실을 조합한 가로줄노린재의 유인효과는 집합페로몬 105㎎+청자콩>105㎎+검정콩>105㎎+태광콩>80㎎+청자콩>80㎎+검정콩>50㎎+청자콩>80㎎+태광콩>50㎎+검정콩>50㎎+태광콩의 순으로 높았다. 유인트랩에 따른 가로줄노린재의 유인효과는 통발트랩>펀넬통기트랩>펀넬트랩>풍뎅이트랩 순으로 높았다. 따라서 콩 포장에서 유인물을 이용한 톱다리개미허리노린재와 가로줄노린재의 유인은 톱다리개미허리노린재의 집합페로몬 4성분과 가로줄노린재의 집합페로몬 105㎎을 청자콩 종실과 조합하는 것이 가장 효과적 이었다.

아가, 청자, 대원의 세 가지 콩 품종이 톱다리개미허리노린재의 발육과 생식이 미치는 영향을 실내에서 비교하였고, 또한 이 세 가지 품종 간에 야외 발생소장을 비교하였다. 실내 실험에서 약충의 발육기간은 아가, 청자, 대원콩에서 각각 평균 14.5, 14.0, 14.6일이였고, 약충의 사망률은 각각 40.0, 51.3, 55.7%였다. 발육기간에서는 통계적 차이가 없었지만, 사망률에서는 아가콩에서 유의하게 다른 두 품종보다 낮았다. 성충의 생식능력으로 7일 동안 암컷이 산란한 알의 총 개수의 평균은 각각 67.8, 68.0, 67.7개였으며 통계적 차이는 없었다. 야외조사는 안동시 풍산면 소재 친환경 인증 포장에서 아가, 청자, 대원을 각각 3반복의 라틴방각법으로 배치하여 재배하였다. 7월 13일부터 10일 간격으로 8회 동안 알, 약충, 성충의 수를 각 처리구에서 임의로 30주를 조사하였다. 조사기간 전체 동안 발생 수는 알의 개수가 아가, 청자, 대원콩에서 각각 평균 18.3, 23.0, 23.7개, 약충은 각각 평균 8.0, 11.3, 17.3마리, 성충은 각각 평균 7.3, 21.3, 15.7마리가 조사되었다. 톱다리개미허리노린재의 알과 약충에서는 통계적인 차이가 없었지만, 성충은 아가에서 가장 적게 발생하였다. 이상의 결과로 새로운 품종인 아가콩은 다른 두 품종에 비해 톱다리개미허리노린재의 유충 생존율을 높였지만 성충의 발생은 다른 두 품종에 비해 적음을 알 수 있었다.

Male adults of bean bug, Riptortus clavatus Thunberg (Heteroptera: Alydidae), release aggregation pheromone (AP) attracting both sexes of adult and nymphs, which its egg parasite, Ooencyrtus nezarae (Hymenoptera: Encyrtidae) exploits the pheromone to find host. The AP consists of three components, (E)-2-hexenyl (Z)-3-hexenoate (E2HZ3H), (E)-2-hexenyl (E)-2-hexenoate (E2HE2H), and tetradecyl isobutyrate (TI). We analyzed composition of the pheromone components of bean bugs from different geographical locations of Korea and Japan. The attractiveness of different blends of AP components to R. clavatus was also tested in the fields in Jinju, Korea and in Kumamoto, Japan. Composition ratios (E2HZ3H: E2HE2H:TI) of the AP of Jinju and Iksan populations were 1:1.4:0.2 and 1:0.8:0.2, and those of Tsukuba and Kumamoto populations were 1:2.8:0.2 and 1:1.5:0.1, respectively. In field tests, traps baited with ratio of 1:1:1 (E2HZ3H:E2HE2H:TI = 16.7:16.7:16.7 ㎎/rubber septum) and 1:1:0.5 (E2HZ3H:E2HE2H:TI = 20:20:10 ㎎/rubber septum) attracted significantly greater number of adult bugs than that of 1:5:1 (E2HZ3H:E2HE2H:TI = 7.1:35.7:7.1 ㎎/rubber septum).

Environmental Enrichment (EE) alone is not capable of enhancing the fine digit and the forelimb functions. Therefore, we applied modified constraint-induced movement therapy (mCIMT) under the influence of EE to assess its effect on promoting improved forelimb sensorimotor functions. Focal ischemic brain injury was produced in Sprague-Dawley rats (60 rats, 250±50 g) through middle cerebral artery occlusion (MCAO). Before MCAO induction, all rats were trained in modified limb placing tests and reaching tasks for 1 week. Then they were randomly divided into three groups: Group I: application of standard environment (SE) after MCAO induction (n=20), Group II: application of EE after MCAO induction (n=20), Group III: MCAO+EE, mCIMT and task-oriented training that was initiated at 10th day after MCAO induction (n=20). We also applied mCIMT (between 9 AM and 5 PM/daily) which included restraining the forelimb ipsilateral to the lesion using the 'Jones & Schallert' method. We assessed the change of modified limb placing, single pellet reaching test and the immunoreactivity of BDNF by immunohistochemistry (pre, 1st, 5th, 10th and 20th day). Group I showed no improved outcome, whereas group II and III significantly improved on the use of the forelimb and the immunoreactivity. The qualitative analysis of the skilled reaching test, of group III showed the greatest improvement in the fine digit and the forelimb function. These results suggest that EE combined with mCIMT is more functional in promoting enhanced fine digit and forelimb functional movements.

Two hymenopteran egg parasitoid species, Gryon japonicum (Ashmead) (Scelionidae) and Ooencyrtus nezarae Ishii (Encyrtidae), were emerged from eggs of bean bug, Riptortus clavatus Thunberg (Heteroptera: Alydidae). The former parasitoid is first recorded in Korea. Brief morphological and biological characteristics, and parasitism on R. clavatus eggs are described.

The objective of this study was to determine the effect of dietary oils on the levels of the γ-linolenic acid in chicken meat lipids. Three hundred ten five, 1-d old, male, Ross strain, broiler chicks were fed for 35 d to compare diets containing evening primrose oil(EPO) and hemp seed oil(HO) to a control diet. Fatty acid composition of lipid from chicken skin, thigh and breast muscle were determined at the end of the trial. The level of γ-linolenic acid of lipids from chicken meat fed diets containing EPO or HO was significantly higher than that of the control group(p〈0.05). The level of γ-linolenic acid of lipids from chicken skin was highest in the group, which had been fed the EPO 0.85%, followed in order by EPO 0.7%, 0.5%, EPO mixed oil, HO and HO mixed oil. There was a significant difference in the level of γ-linolenic acid of chicken skin between the control and treatment groups(p〈0.05). The level of γ-linolenic acid of lipids from chicken thigh muscle was also similar to skin, and significantly higher than that of the control group(p〈0.05). The level of γ-linolenic acid of lipids from chicken breast muscle was highest in the group, which had been fed the EPO 0.5%, followed in order by EPO 0.7%, 0.85%, HO 0.5% and HO mixed oil. There was a significant difference in the level of γ-linolenic acid of chicken breast muscle between the control and treatment groups(p〈0.05).

Life history of Ivela auripes (Lepidoptera: Lymantriidae), a pest of Comus controversa, was investigated both in laboratory and field condition. At 24±1℃, developmental period from larvae to adult and adult life span was 26.3±0.3 and 4.4±0.2 days, respectively. Developmental period of pupae reared in the laboratory was not significantly different from those collected in the field. Female moths reared in the laboratory laid significantly less eggs than those emerged from the pupae collected in the field. Light trap catches was less effective than direct monitoring in the field. The two monitoring results showed that adult moth emerged from early June to late June, and the time of 50% cumulative emergence was 17 June.

To improve trunk stability, various exercise protocols were introduced into the clinical field. Trunk and lumbar stability exercises on unstable surfaces are especially recommended to improve lumbar stability. The purpose of this study was to compare abdominal oblique muscle activity during leg raising in hook-lying position among 3 different type of surface conditions (on floor (F), vestibular board (VB), and foam roll (FR)). Sixteen able-bodied volunteers, who had no medical history of lower extremity or lumbar spine disease, were recruited for this study. Surface electromyography (EMG) activity was recorded from the internal and external oblique muscles of both sides. The normalized EMG activity was compared using a one-way repeated ANOVA. The results showed that the EMG activities of the internal oblique and external oblique of the lifted leg side during straight leg raising significantly increased under the FR condition when compared to the F condition. There was no significant difference of the EMG activity in abdominal oblique muscles between the VB and the FR conditions. The EMG activity of the internal oblique of supported leg side during the straight leg raising was significantly greater under the FR condition than the VB and F conditions (p<.05). The composition ratio of EMG activity of internal oblique muscles during straight leg raising was significantly increased under the FR condition. Therefore, straight leg raising exercise on foam roll in hook lying position could be beneficial to improve trunk and lumbar stability.

The effect of food source in aggregation pheromone trap of bean bug, Riptortus clavatus, was evaluated in terms of the trap attractiveness using water-pan and cylinder-type traps. Water-pan trap baited with pheromone+food (dried soybean+dried peanut+water) attracted significantly more number of females and males than those with pheromone or control trap. Although cylinder-type trap baited with pheromone+food did not show significantly higher attractiveness, it attracted more adults and nymphs than the pheromone and control traps. Further research on the effect of trap types on attractiveness is needed.

망사시험관을 이용하여 20, 24, 28 및 에서 톱다리개미허리노린재의 난 및 약충발육과 성충수명 및 산란수를 조사하였다. 부화율은 에서 100%였으며, 이 온도에서 멀어질수록 부화율은 낮아졌다. 난기간은 에서 7일 이었으며, 온도가 낮아질수록 날기간이 길어져 에서 16.7일이었다. 영기별 발육기간은 1령에서 일로 가장 짧았으며, 영기가 증가할수록 발육기간이 점점 길어져 5령에서 일로 가장 길었다. 약충기간은 20, 24, 28 및 에서 각각 38, 30, 23 및 18일 이었다. 우화율은 20, 24, 28 및 32에서 16, 41, 72 및 68% 이었다. 암컷 및 수컷성충의 수명은 에서 각각 63 및 60일로 가장 길었고, 에서 각각 20 및 19일로 가장 짧았다. 산란전기간은 에서 11일로 가장 길었으며, 온도가 높아질수록 현저하게 짧아져 에서 5일로 가장 짧았다. 산란수는 에서 67개로 가장 많았고, 에서 21개로 가장 적었다. 발육임계온도는 알의 경우 였고, 1, 2, 3, 4 및 5령충은 각각 9.3, 12.7, 10.0, 11.0 및 였다.

콩 생식생장 단계별로 톱다리개미허리노린재를 접종하거나, 침으로 꼬투리를 치르는 인위적 가해를 통해 콩 종자 피해를 조사하였다. 착협기에 톱다리개미허리노리재 성충을 섭식시켰을 때, 빈 꼬투리와 종자로 전혀 발육하지 못한 판형종자의 생성비율이 종실비대성기에서의 가해보다 높은 것이 관찰되었다. 종실비대성기에 섭식시켰을 때는 종자모양은 거의 정상적이나 종피에 피해흔적이 뚜렷한 종자 수가 유의적으로 증가하였다. 종자모양이 많이 변형되거나 불완전하게 성숙한 기형종자의 비율은 처리 사이에 차이가 없었고 다른 종자형태들에 비해 상대적으로 낮은 비율을 차지하였다 착협시 피해에서 생성된 판 종자의 83%, 종실비대성기에 생성된 피해흔적이 있는 종자의 91%에서 종자 표면 위에 실험곤충의 빨대피막이 관찰되었다. 개화기 섭식은 피해종자 생성에 거의 영향을 주지않았다. 종실비대성기와 성숙기에 침을 이용하여 콩 꼬투리를 찔러 물리적인 가해를 하였을 때, 수확기에 건전한 종자들을 발견할 수 없었고, 피해gms적이 있는 종자의 비율이 기형종자 보다 많았다. 종실비대성기에 침으로 찌른 회수가 많은 경우 판형종자와 기형종자의 비율이 증가하였고, 피해흔적 종자 비율은 상대적으로 감소하였는데 무게감소 비율은 적게 찌른 경우 보다 상대적으로 켰다. 그러나 성숙기 인위적 가해에서는 수확종자의 무게감소가 나타나지 않았다.