복숭아심식나방(Carposina sasakii)은 한국, 일본 및 중국 동북부 지방에 분포하며 사과, 배, 복숭아 등 수출관련 과수의 중요한 식물검역해충이다. 방사선 처리에 의한 수출과실의 복숭아심식나방 소독을 위하여 살충 및 발달억제에 필요한 방사선 조사량을 분석하였다. 복숭아심식나방 유충이 심식한 사과를 채집하여 0, 100, 150, 200, 250, 300Gy의 감마선을 처리하였다. 감마선 처리한 과실을 실온에 보관하면서 과실로부터 유충의 탈출율, 용화울, 우화율을 측정하였다. 유충의 탈출율은 무처리를 기준으로 했을 때에 각 조사량별로 100Gy(85.8%), 150Gy(55.4%), 200Gy(27.9%), 250Gy(13.2%), 300Gy(5.2%)로 나타났다. 용화율은 100Gy(48.8%), 150Gy(21.4%), 200Gy(7.7%), 250Gy(1.1%), 300Gy(0%)이고 우화율은 100Gy(5.4%), 150Gy(0%), 200Gy(0%), 250Gy(0%), 300Gy(0%)였다. 즉, 감마선 처리에 의한 복숭아심식나방 유충의 살충율은 300Gy조사했을 때에 95%였으며 우화 억제율은 150Gy 조사했을 때에 100%로 나타났다. 또한, 100Gy 조사했을 때에 일부 성충이 우화하였지만(5.4%) 산란율은 0%였다. 본 연구 결과를 토대로 복숭아심식나방과 같은 심식류 검역해충의 방제를 위하여 방사선 처리가 효과적으로 활용될 수 있다고 판단된다.

시중에 유통 중인 영양보충용 제품의 수용성 비타민 B1(thiamin), B2(riboflavin), B3(nicotinic acid and nicotine amide), B6(pyridoxine), C(ascorbic acid)의 신속한 동시분석 방법을 확립하기 위하여 본 연구를 실시하였다. 영양보충용 제품은 정제, 연질캅셀, 분말, 액상의 4가지 제형에 대해 27종의 제품을 구입한 후, Ion-pair 분리기법을 사용하여 HPLC-UVD를 이용한 동시분석 방법을 검토하였다. 비타민 B1, B2, B3, B6, C의 HPLC에 의한 동시분석 조건을 검토한 결과, 이동상은 0.02% triethylamine, 17.5% 메탄올, 5 μM sodium hexanesulfonic acid가 함유된 pH 3.5(acetic acid로 조절)의 수용액을 사용하였고, 용출시간은 다른 피크의 영향을 받지 않도록 30분으로 하였다. 수용성 비타민의 회수율은 96% 이상이었다. 본 연구에 의해 확립된 수용성 비타민의 동시분석 조건은 검량선의 직선성, 정밀성, 정확성, 기기적합성 등이 USP 및 ICH 기준에 적합하여 HPLC의 동시분석 방법으로 합당하였다. 수용성 비타민의 추출 용매는 제형에 따라 약간의 차이를 보이긴 했으나, 물이나 산성조건을 갖춘 HPLC의 이동상이 에탄올이나 메탄올보다 높은 추출 효율을 보였다. 초음파 추출기에 의한 추출 시간은 20분이 가장 적당하였다. 본 연구의 결과는 수용성 비타민의 신속한 추출 및 분석에 매우 효율적으로 이용될 것으로 기대된다.

The objective of this study was to compare the accuracy analysis and the effect of field application of a newly developed automatic heat detector in dairy cows. From 2009 to 2010, we used 48 Holstein cows (mounting cows : 38 heads, standing cows : 10 heads) raised in experimental barn of National Livestock Research Institute (RDA) for the accuracy analysis of automatic heat detector, and 14 Holstein cows raised in three commercial dairy farms of Cheonan and Pochun area for comparison of the effect of field application. The accuracy of response in cows attached with automatic heat detector was 86.8% (33/38) displayed on board when mounting activity observed, and 100% (10/10) when standing activity observed, and on average, 90.0% (43/48) displayed on board. The accuracy of automatic heat detector in on-farm test was 85.7% (12/14), and conception rate was 75.0% (9/12).

The purpose of this research was to study food behaviors and nutrient intakes according to body mass index(BMI) and body image recognition in 211 female university students in Incheon. The majority of the surveyed subjects were in the normal range for BMI. Most of these female university students preferred a thin body type and were not satisfied with their body shapes. Most also controlled their body weight for appearance by exercising and limiting food intake. The obese group often ate processed foods(p<0.01) and ate out(p<0.05). The underweight group and normal-weight group took vitamins and supplements more frequently(p<0.001). Those students who recognized their body image as a ``fat shape`` had good appetites(p<0.05) and those who recognized themselves as ``normal shape`` considered food formulations ate every meal (p<0.05) and consumed vitamins and other supplements frequently(p<0.01). The mean calorie intake of all students was about 80% of the recommend amount, so there was a risk of lack of various nutrients. The ``fat`` students by body image recognition had lower calorie intakes in order to reduce body weight. In all the groups, there was a lack of vitamins B1 and B2, as well as the minerals Ca and Fe. Therefore, education on correct body image recognition and training on proper weight control are deemed necessary. In addition, education and counseling on nutrition that are tailored to individual status are needed.

The purpose of this study was undertaken to evaluate of cryopreservation efficiency in α 1,3-galactosyltransferase knock-out(GalT KO) cloned miniature pig sperm. To compare ability of frozen-thawed sperm characteristics, three different pig strains (GalT KO) cloned miniature pig, PWG miniature pig and Duroc were used. The ejaculated semen from the three pig species was diluted with same volume extender and added to LEY solution for freezing. The diluted semen was placed in 0.5 ml straws, and freezing was initiated by exposing the straws to liquid nitrogen (LN2) vapours for 10 min before placing them into LN2 for cryopreservation. A fter thawing, the sperm ability were assessed for viability (SYBR-14/PI staining), abnormality (Rose Bengal staining), and acrosome status (intactness, intensity and capacitation) (chlorotetracycline, CTC staining). The viability of frozen-thawed GalT KO pig sperm had no significant difference as compared with Duroc and PWG miniature pig sperm. However, The CTC pattern of frozen-thawed GalT KO cloned miniature pig spermatozoa showed significantly lower rates in F pattern and AR pattern (p<0.05) and significantly higher rates in B pattern than Duroc and PWG miniature pig (p<0.05). The abnormality of GalT KO cloned miniature pig sperm was significantly lower as compared to Duroc and PWG miniature pig sperm (p<0.05). In conclusion, GalT KO cloned miniature pig semen can be cryopreserved successfully and used for artificial insemination reasonably.

The objective of this study was to compare the anticancer activity of glucosinolates against four different cancer cells; SNU-251, SNU-354, SNU-C4, MCF-7 and to determine the amounts of glucosinolates in mustard leaves. Green and red mustard leaves were cultivated on the field of Rural Development Administration from May to July, 2010. After the leaves were harvest and lypophilized, the fine powder was extracted with boiling 70% methanol(v/v) for the measurement of anticancer activity and then applied to the cancer cells obtained from Korean Cell Line Bank(KCLB). The anticancer activity of red leaf mustard was higher than that of green mustard leaf, particularly in SNU-251 for 24 hr and in SNU-C4 for 48 hr. In both green and red mustard leaf, sinigrin was the most abundant glucosinolte and was determined as 4.71 and 3.06 ㎎/g dry wt., respectively, but glucoiberin only presented 0.14 ㎎/g dry wt. with minor amounts.

Natural Omija belongs to magnoliaceae was known to possess natural odor, taste, color, and various pharmaceutical & chemical characteristics. Omija extraction was extracted using ethanol as a solvent. Omija extract showed a light red-violet color of viscous liquid state. Some conclusions from the result of characteristic experiment were obtained as follows. From the result of antimicrobial experiment, occurrence of staphylococuss aureus and aspergillus niger as microbes was decreased according to the passage of time. This phenomenon could know that Omija component is affected to antimicrobial effect. From the result of dye experiment, fiber dyeing showed with some ivory color after dyed to cotton and silk. This phenomenon could know that Omija component is affected to dyeing effect from observation of scanning electron microscope(SEM). From the result of instrument analysis, inorganic components of K(107.30ppm), Na(2.110ppm), Ca(0.935ppm), Mg(0.891ppm), Li(0.270ppm) etcs from Omija were detected with ICP/OES, and aromatic components of benzene(10.808), a-pinene(13.996), phenol(14.183), β-terpene(15.840), a-terpinolene(17.616) etcs from Omija were also detected with GC/MSD.

This study established hazards which may cause risk to human at farm during cultivation stage of paprika. Samples of plants (paprika, leaf, stem), cultivation environments (water, soil), personal hygiene (hand, glove,clothes), work utensils (carpet, basket, box) and airborne bacteria were collected from three paprika farms (A, B, C)located in Western Gyeongnam, Korea. The collected samples were assessed for biological (sanitary indications and major foodborne pathogens), chemical (heavy metals, pesticide residues) and physical hazards. In biological hazards,total bacteria and coliform were detected at the levels of 1.9~6.6 and 0.0~4.6 log CFU/g, leaf, mL, hand or 100 ㎠,while Escherichia coli was not detected in all samples. In major pathogens, only Bacillus cereus were detected at levels of ≤ 1.5 log CFU/g, mL, hand or 100 ㎠, while Staphylococuus aureus, Listeria monocytogenes, E. coli O157and Salmonella spp. were not detected in all samples. Heavy metal and pesticide residue as chemical hazards were detected at levels below the regulation limit, physical hazard factors, such as insects, pieces of metal and glasses, were also found in paprika farms. Proper management is needed to prevent biological hazards due to cross-contamination while physical and chemical hazards were appropriate GAP criteria.

2010년 4월 20일, 반잠수식 시추선 Deepwater Horizon호가 폭발, 침몰하는 사고가 발생하였으며, 이로 인해 490만배럴(약 77.8만톤)의 원유가 미국 멕시코만으로 유출되었다. 이 사고로부터 1년 이상이 경과함에 따라 정부 측과 오염행위자 측의 각종 분석보고서와 사고로부터 얻은 교훈 등이 발표되고 있다. 본 연구에서는 미국 버락 오바마 대통령의 지시로 구성된 "Deepwater Horizon 기름유출과 원해 석유시추에 관한 국가위원회"의 최종보고서와 미국 해안경비대(USCG)와 미국 에너지 관리 규제 집행국(BOEMRE) 합동조사반의 중간보고서를 바탕으로 기름오염 사고 원인과 사고대응에 대한 측면을 중점적으로 검토 분석하였다. 또한, 분석결과를 토대로 우리나라 정부에서 유출구 봉쇄조치 지도감독 능력 강화, 현장소각과 임시방제정 프로그램의 도입검토 및 향후 미국의 연구개발성과에 대한 지속적인 모니터링 등 국가방제정책의 개선방안을 제시하였다.

KFDA compiles the statistical data of food poisoning outbreaks two or more persons since 2002in Korea and release them to the public on the web. There is a gap of outbreak number between the real situation and the reports. To reduce the gap, addition of sporadic individual case of food poisoning may be one of the solution method. The statistical data of Japan where food consumption pattern is similar to Korea, were used in this study to compare the ratio and the pattern between the outbreak cases two or more persons and individual cases. By doing so,the data of Japan regarding to outbreak cases two or more persons will be comparable to that of Korea. The data of 2002 and 2003 in Japan showed that sporadic individual cases were 43.3% in the total food poisoning cases. The individual cases occurred highly in unknown places (90-92.3%) and home (6.2-8.5%) whileas the outbreaks two or more persons occurred mostly in the place of restaurants (46.6-50.1%) and inns (9.2-9.8%). The food-borne pathogens attributed to the individual cases were C. jejuni (51.9%), Salmonella spp. (35.3%), and V. parahaemolyticus (9.8%)while those to the outbreak cases two or more persons were norovirus (31.3%), Salmonella spp. (20.8%), C. jejuni (15.5%) in Japan. The data of 2002-2009 between Korea and Japan showed the outbreak case report rate was 1:1.5based on the total population number.

The objective of this study was to find out candidate genes associated with litter size trait in pigs of inbred Large Yorkshire and Landrace populations. 86 sows were screened for candidate genotypes along with litter size data recordings. Association of litter size with genotypes of candidate genes were investigated to verify the usefulness of each gene's genotypes as markers for the trait. For the lines of Large Yorkshire, PRLR3 and RBP4 genes were genotyped. Frequency distribution of PRLR3 with genotypes AA, AB and BB were each 0.14, 0.44 and 0.42. And the average litter size by PRLR3 genotypes were 8.83, 10.81 and 10.70 piglets per litter, the average estimated breeding values of which were 0.243, 0.332, 0.365, respectively for AA, AB and BB genotypes. Genotypic frequencies of RBP4 by AA, AB and BB genotypes were 0.10, 0.44 and 0.46. The average litter size by genotypes of RBP4 were 10.40, 10.57 and 10.35 piglets per litter and their corresponding average estimated breeding values were 0.451, 0.353 and 0.261, respectively for genotypes AA, AB and BB. Significance in differences among genotypes were not observed, but B allele of RBP4 seems to be associated with litter size. In Landrace lines, frequencies of RBP4 genotypes, AA, AB and BB were 0.29, 0.55 and 0.16. And the average litter size of these genotypes were 10.50, 11.08 and 11.00 piglets per litter. The corresponding averages of estimated breeding values of each genotypes were 0.172, 0.135 and 0.104. In Landrace lines, allele A was more likely to be associated with litter size, even if differences among average litter size were not significant. We conclude that genotyping of two candidate genes is a helpful tool to identify genetic potentials of litter size in pigs.

우리나라는 선박관리산업의 중요성을 인식한 해운업계 및 학술단체의 전문가들에 의해 지속적으로 노력한 결과 최근 국회의원의 발의에 의하여 "선박관리산업발전법안"이 제안되었다. 이러한 성과는 우리나라의 선박관리산업이 선진국 대열에 진입하게 되는 결정적인 계기가 된다고 생각한다. 이 연구는 최근 발의된 우리나라 "선박관리산업발전법안"을 각 조문별로 소개하고, 조문별 분석을 통해 법적인 문제점과 실효성을 검토하여 개선방안을 제시함으로써 향후 우리나라 선박관리산업의 발전을 위한 법과 제도의 발전에 기여하는 것을 궁극적인 목적으로 한다. 이를 위해 우리나라 "선박관리산업발전법안"의 구성, 목적, 용어의 정의, 다른 법률과의 관계 등을 중심으로 개념을 소개하고, 이 법률안의 핵심이라고 할 수 있는 선박관리산업의 육성 및 지원, 그리고 선박관리우수사업자의 인증을 중심으로 구체적 실효성을 분석하였다.

하절기 수화발생이 빈번한 대청호에서 2003~2005년(3년)에 걸쳐 분자생태학적 방법의 하나인 DGGE를 이용하여 시간에 따른 미생물 군집구조의 변화를 연구하였다. 조사기간 동안 출현한 식물플랑크톤을 형태학적으로 분류한 결과 Cyanophyceae가 우점하였고, 이중에서 Microcystis, Planktothrix (Oscillatoria), Phormidium 그리고 Anabaena 속이 크게 우점하였다. 분자적 군집분석 방법으로서 16S rDNA

Poly(vinyl chloride)-g-poly(oxyethylene methacrylate) (PVC-g-POEM) 가지형 공중합체를 원자전달라디칼 중합을 통해 합성하여 전기변색소자의 전해질에 적용하였다. 가소화된 고분자 전해질은 가소제로서 propylene carbonate (PC)/ethylene carbonate (EC) 혼합물을 도입하여 제조하였으며, Lithium tetrafluoroborate (LiBF4), lithium perchlorate (LiCIO4), lithium iodide (LiI) and lithium bistrifluoromethanesulfonimide (LiTFSI)를 사용하여 염의 종류에 따른 영향을 조사하였다. 광각 x-선 산란(WAXS)과 시차주사 열량법(DSC) 측정 결과 고분자 전해질의 구조와 유리전이온도(Tg)가 변하였고, 이는 POEM 내의 에테르의 산소와 리튬염 사이의 상호작용으로 인해 변했다는 것을 FT-IR 분광법을 통하여 확인하였다. 투과전자현미경(TEM) 측정 결과 PVC-g-POEM 가지형 공중합체의 미세상분리 구조가 PC/EC와 리튬염의 도입에도 변하지 않는 것을 관찰하였다. 가소화된 고분자 전해질은 poly(3-hexylthiophene) (P3HT) 전도성 고분자를 이용한 전기변색소자에 적용되었다.

Deoxynivalenol (DON) and zearalenone (ZEN) are mainly contaminated mycotoxins in feeds. The study was carried out to analyze and survey the contamination of DON and ZEN in one hundred thirteen samples of feeds. After cleaning all samples with immunoaffinity column, the mycotoxins were analysed by using high performance liquid chromatography/fluorescence with diode array detector (HPLC /FLD with DAD). The average recoveries of DON were 88.76 and 95.40% at the levels of 200 and 1,000 μg/kg and 87.09 and 98.40% of ZEN were recovered at the levels of 100 and 500 μg/kg, respectively. The limit of detection (LOD) were 6.0 and 3.0 μg/kg for DON and ZEN, respectively. The average concentrations of DON were 372.1, 324.0 and 990.9 μg/kg in chicken, pig and cattle feed, respectively. Those of ZEN were 76.1, 43.7 and 196.2 μg/kg for them, individually.

목적: 시야검사 시 신뢰도 지수에 영향을 미칠 수 있는 여러 요인들을 정상인과 녹내장환자를 대상으로 비교 분석하였다. 방법: 녹내장이 없는 정상(normal subjects)군 158안과 녹내장 판정을 받은 녹내장환자군 64안으로 나눠서 각각 험프리 자동시야검사계(Carl Zeiss Meditec, Dublin, CA, USA)를 이용하여 검사하였다. 낮은 신뢰도의 검사결과는 신뢰지수를 분석하여, 주시상실도 20% 이상, 허위 양성률 33% 이상, 허위 음성률 33% 이상 등 세 가지 조건에 어느 한 가지라도 해당되면 낮은 신뢰도로 정의하였다. 결과: 총 222안의 실험군 중 정상군(158안)에서는 35안(22%)에서 낮은 신뢰도를 보였으며 녹내장 환자군(64안)에서는 16안(25%)에서 낮은 신뢰도를 보였다. 이들을 재검사 하였을 때 정상군의 35안 중 16안(46%)에서 다시 낮은 신뢰도를 보였고, 녹내장 환자군에서는 16안 중 5안(31%)에서 다시 낮은 신뢰도를 보였다. 처음 검사 시 낮은 신뢰도를 보이는 대상이 재검사시 다시 낮은 신뢰도를 보이는 경우를 알아본 결과 정상군에서는 46%, 녹내장 환자군에서는 31%가 다시 낮은 신뢰도를 보였다. 결론: 자동시야검사라 하더라도 검사진행시 반드시 검사자가 옆에서 주의를 관찰하면서 시야검사를 진행하는 것이 필요하다고 사료된다.

Rice has been the most important staple food in everyday meals of Korean people for thousands of years. Nowadays, it is getting increasingly used as flour ingredients in a variety of processed foods, so that it is consumed in more diversified ways. As a consequence, production volume of rice flour to manufacture rice cakes, noodles,breads, or confectioneries is recently getting increased in Korea. But there are not sufficient research outcomes to guarantee Korean consumers microbiological qualities of rice flour as well as rice. As a preliminary experiment,therefore, the microbiological profiles (aerobic mesophilic bacteria (AMB), spore-forming aerobic bacteria (SAB),lactic acid bacteria (LAB), yeasts and molds (YM), and Escherichia coli and coliforms) have been monitored for nine retailed white rice samples in this study. AMB counts ranged 10² - 10^6CFU/g for all the nine white rice samples. All the nine rice samples have SAB counts within a narrow range (1.0 × 10²- 2.5 × 10³CFU/g). LAB was detected in two white rice samples (4.0 × 10² and 3.7 × 10³ CFU/g). YM was detected in one white rice sample (2.0 × 10² CFU/g)only. E. coli was not detected from all the nine samples. Coliforms were detected in one white rice sample (4.1 × 10CFU/g) only. All the rice samples were conclusively considered to have various microorganisms, though most of them are harmless and some, such as coliforms, may be harmful.