불연속 갤러킨 정식화에 기초를 둔 시간적분법에 대하여 시간을 변수로 한 유한요소적 접근법을 시도하였다. 단일 형상함수와 두 형상함수 정식화에 대해 각각 선형, 이차 형상함수를 적용하여 모두 네 종류의 시간적분법을 유도하였으며, 각 방법에 대하여 시간시텝의 증가에 따른 변위와 속도의 관계를 나타내는 증폭행렬을 계산하였다. 유도된 방법들의 성능을 평가하기 위하여 부하가 갑자기 변화는 진동 문제를 해석하고 변위의 오차를 비교하였다. 네 가지의 방법에 대하여 국부 오차 추정치를 개발하였으며, 오차 추정치의 정확도를 수치예를 이용하여 평가하였다. 단일 형상함수 정식화에서 이차 형상함수를 이용한 오차 추정치가 실제 국부오차를 잘 나타내었으며 유도된 오차 추정치는 시간간격제어 기법에서 시간간격의 크기를 결정하는 척도로 이용 가능하다.

Ti-45at%AI-1.6at%Mn 조성을 갖는 금속간화합물의 장시간 및 반복산화 거동을조사하기 위하여 반응소결법 및 플라즈마 아크 용해법으로 제조한 시편에 대하여 800˚C에서는 반응소결재와 용제재 모두 등온 및 반복산화에 대하여 우수한 저항성을 나타내었다. 900˚C에서는 반응소결재의 경우에는 등온 및 반복산화에 대하여 우수한 저항성을 보였으며, 중량변화와 산화피막의 박리는 극히 적었다. 이에 비해 용제재의 경우에는 등온 및 반복산화에 의해 중향이 크게 변하였으며 피막의 박리도 극심하였다. 900˚C에 있어서 두 재료간의 이러한 산화거동 차이는 기지/산화물 계면 부근에 형성된 산화층의 차이에 기인하는 것으로 간주하였다. 반응소결재의 경우에는 계면 부근에 연속적인 AO3O3층이 형성되며, 이러한 층이 산화에 대한 보호막으로 작용하는데 비하여 용제재에 있어서는 계면 부근에 AO3O3와 TiO2의 혼합층이 형성되었다. 용제재의 반복산화시에 보여진 피막의 박리는 냉각시에 TiO2와 기지간의 열팽창계수 차이에 기인하여 발생하는 열응력을 TiO2가 견디지 못하고 박리를 초래한 것으로 해석하였다.

This study was undertaken in an effort to develop a cryopreservation system of immature and mature porcine oocytes. For this aim, the experiments were designed to examine the effect of cryoprotectants and equdibration time on the viability of frozen-thawed oocytes by using trypan blue(TB) and fluorescene diacetate(FDA) test. The viability of frozen immature oocytes evaluated by TB test was slightly higher than that of frozen mature oocytes. The viability(25.O%) after IVM of frozen-thawed immature oocytes greatly decreased that(42.9%) of oocytes just after thawing, but it was higher than frozen-thawed mature oocytes(15.8%). When immature oocytes were equilibrated for 10, 20 and 30 minutes before freezing the oocyte viability was 20.0, 31.3 and 42.9%, respectively. There was a tendency for long equilibration before oocyte freezing to be more effective for the immature oocytes and a short equilibration time for mature oocytes. Although there was no difference in viability index of frozen oocytes hetween the viability test methods, the index of TB test was slightly higher than that of FDA test. The viability(FDA test) of frozen-immature oocytes with 3 different crtoprotectants was 22.2% for propylene glycol(PG), 9.3% for polyehtylene glycol(PEG) and 65.6% for PG+PEG, in which PG+PEG was more protective against freezing effect.

Experiments were conducted to assess the effect of quality and viability of bovine blastocysts derived from in-vitro culture(IVC) of in vitro matured and fertilized(IVM-IVF) oocytes during their transport 2 hours. Follicular oocytes were collected form ovaries obtained at a slaughterhouse and were cultured for 24 hours in TCM-199. The IVM oocytes were fertilized in vitro with caudal epididymis spermatozoa. Fertilized oocytes were cultured for 7 to 9 days, and embryos that developed to the blastocyst stage were used for the experiment. The blastocysts, packed in straws with storage medium that consisted TCM-199 with HEPES equilibratd in air and supplemented with 10% FCS were transported at 39~(2.0 h). The quality of blastocysts was assessed and ranked as A(excel-lent), B(Good), fair or poor after transportation. The percentages of A and B grade blastocysts after transport duration for < 1 hours(97.7%) were similar to the result from transport duration for 1~2 hours (92.9%) and 2~3 hours(89.6%), but significantly(P<0.05) higher than transpot duration for 3~4 hours(76.3%). The percentages of A and B grade blastocysts after transport duration for two hours from developed blastocyst at 7day(100%) and 8day(85.0%) were higher 9day(96.6%) and >9day (40.0%). And early to expanded blastocyst produced in vitro were transferred to recipient cow by additional embryos at 7 and 8th day after AI. Three of them were pregnant to term and produced four twin calves, and two calves was premature birth. The gestation lengths of male to female and female to female twin were 282 and 281 days, respectively. And birth weight of twin calves were male to female(22.Skg) and female to female twin(20.3Okg), respectively.

돌연변이체인 or (orange body) 계통과 KNIH (Korea National Institute of Health) 계통의 바퀴(Blattella germanica (L.) (Orthoptera: Blattellidae)를 반자연적인 조건하에서 비디오와 컴퓨터 자료처리를 통하여 연속적으로 관찰하였다. 휴식처에 머무르거나, 먹고, 물 마시며, 다른 개체들과 교신하는 행동을 관찰 할 수 있는 사각형의 관찰상자 내에서 수컷 성충을 개체별로 4~5일 동안 연속적으로 관찰하였는데, 본 논문에서는 휴식처에 머무르는 시간과 방문 빈도를 분석하였다. 개체들 간의 변이가 상대적으로 컸지만 시험개체들은 휴식처에 전체 관찰시간의 47 -61%를 평균적으로 머무르는 것으로 나타났다. 판찰 개체가 휴식처에서 머무르는 시간은 모계에 따른 유의한 차이가 있었으나, 계통 사이에는 뚜렷하게 구분이 되지 않았다. 명암기의 차이는 두 계통 모두 관찰되었는데 휴식처에 머무르는 시간은 암기보다 명기에서 더 길었다. 휴식처 주변의 국소적 활동성을 나타내주는 방문빈도는 KNIH 계통보다 or 계통에서 상대적으로 높게 나타났는데, 모계와 계통간에 유의한 차이가 있었다. 방문빈도의 명암기 차이는 or 계통에서는 유의하게 나타났지만 KNIH 계통에서는 없었다. 개체들 간의 변이가 있었으나 동일한 모계 내에서 휴식처에 머무르는 시간이나 방문빈도가 유사하게 관찰되는 개체들이 있었다.

The objective of this study was to identify pulmonary functional variations in relation to postural changes, lapse after changing position, and the use of abdominal band in the cervical cord injured. The subjects of this study were 19 quadriplegic patients who had been admitted to the department of the Rehabilitation Hospital, College of Medicine, Yousei University, from April, 1997 through May 3, 1997. A spiroanalyzer was used to measure pulmonary function in supine, standing, time after changing position, and recording to the position, application method, and tightness of the abdominal band. The data were analyzed by the repeated measure one-way ANOVA, and Wilcoxon signed rank test. The findings were as follows: 1. All phase of the patients' pulmonary function improved significantly in supine posture in contrast to standing (vital capacity by and expiratory reserve volume by ). 2. The longer the time lapsed from supine posture to standing, the patient's expiratory reserve volume, maximum ventilation volume, vital capacity, and forced expiratory volume increased. 3. When the patient lay in supine position, the maximum ventilation volume, vital capacity, and the forced vital capacity increased then the center line of the abdominal band was placed along iliac crest; on the other hand, when the patient was standing, placing the bottom line of the abdominal band along iliac crest increased the maximum ventilation volume, vital capacity, and forced expiratory volume. 4. In placing the abdominal band in the patients, leaving space between the top and bottom lines of the band helped increased in maximum ventilation volume, vital capacity, and forced vital capacity for patient in supine as well as in standing. 5. When placing the abdominal band to patients in supine posture, reducing the length of the band by 2.5% along the patient's waist line increased the patients' vital capacity, while reducing the length by 10% to patients in standing increased the maximum ventilation volume. The abdominal band should be placed in such a way that the bottom part of the band should be more tightly fastened while leaving enough room for a hand to be placed in between the body and the band for the top part of the hand. It should also be noted that in a supine position, the bottom line of the band should be placed along the iliac crest, while in standing, the center line should be placed along the iliac crest. The length of the band should also be reduced by 2.5% of the waist line in supine position, and in standing, the length should be reduced by 10%. It should also be noted that the pulmonary function of the patients should be measured at least 10 minutes after one position change.