본 연구의 목표는 한의학을 기초로 한 새로운 평가 방법을 이용하여 한방화장품의 효과를 분석하고자 하는 것이다. 한의학에서는 얼굴과 피부에 나타나는 현상을 분석하는 것이 환자의 질병과 체질을 예측하는 중요한 방법이었다. 내부 장기의 상태를 얼굴에 발현되는 증상과 피부를 통해 진단하는 방법을 한의학의 진단체계에서는 ‘망진(望診)’이라 한다. 이에 저자들은 지난 5년간 인삼농축크림을 사용한 피검자군 여성 11명과 대조군여성 11명을 대상으로 한의사 3인의 망진 평가와 비침습적인 피부 실측 과정(non-invasive skin bio-engineering)을 병행하여 두 그룹 간의 피부 상태를 비교하여 유의한 결과를 얻었기에 이를 보고하는 바이다. 총 17항목의 망진 평가에서는 7개 항목에서 인삼농축크림 장기사용군이 대조군에 비하여 통계적으로 유의하게 우수한 결과를 보였으며 7가지 피부 측정항목에서는 유분, 거칠기, 색소침착 면적과 눈가 주름 깊이에서 인삼농축 크림 장기사용군이 대조군에 비해 우수한 결과를 보였다. 본 실험을 통해 인삼농축크림의 장기사용이 피부에 긍정적인 영향을 줌을 확인하였으며, 또한 한의학적인 진단법과 현대기기를 이용한 피부 계측 방법을 융합하여 한방화장품의 특성을 살린 신규 평가 방법을 제안하고자 하였다.

This study was done in order to investigate the bioconversion of ginsenoside, as well as the quality characteristics of fermented ginseng, by using lactic acid bacteria. Quality characteristics such as the thin layer chromatography(TLC) pattern, ginsenosides, total phenolic content, electron donating ability, and total sugar of fermenting ginseng and red ginseng were analyzed. The ginsenoside Rg2r, Rh2s and Rh2r of the fermented ginseng and red ginseng for 65 hours at a temperature of 37℃ were not detected. The ginsenoside Rg1 and Re contents have decreased, while the Rh1, Rg2s, Rd, Rg3r, and Rg3s have increased due to fermentation. The ginsenoside Rg3 of the fermented red ginseng has increased and the contents were 114.83∼131.68 μg/mL (control 104.56 μg/mL). The total phenolic content and electron donating ability of the red ginseng have totally decreased after 7 days of fermentation. The total phenolic contents of the fermented ginseng and red ginseng with different lactic acid bacteria did not show any tendency as different strains. The electron donating ability of the fermented ginseng has increased; however, the electron donating ability of the red ginseng has decreased. The total sugars of the fermented ginseng and red ginseng with different lactic acid bacteria have also decreased.

This study was carried out to have the basic and applied information to develop the cultivation methods and models and to increase the productivity of high-quality ginseng. Plant height was dramatically increased from one year old to four years old, and then it was grown up very slowly. Stem length was shown similar tendency as the plant height did. Its range were from 9.4 cm in one year old to about 42.4 cm in four year-old ginseng. The leaf area was clearly increased until four years old, 10.1cm2 in one year old to 204.9cm2 in four years old, while it was slightly increased after four years old. Root length was continuously increased from one year old until four years old, and then it was grown up very slowly. Tap root length which was measured was appeared about 8 cm from three years old to six years old, showing not much different among the year-olds. The root diameter was continuously increased from one year old to six year-old ginseng, about 2.9 mm and about 19 mm, respectively. The moisture content was higher on the shoot than that on the root. It was slightly decreased as they were getting old. In shoot part, the rate of leaves and stems based on dry weight was about 1 : 1. In the root, the dry weight of tap root was heavier than that of lateral root. The dry weight of shoot was decreased after four years old, while it of the root was continuously increased until six years old, resulting the production of ginseng root. Based on the results of this study, growth characteristics of shoot and root of ginseng were dramatically increased from one year old to four years old and then their growths were appeared different between shoot and root, showing not much growing in shoot and keep growing in root.

This study was carried out to investigate the cause of leaf discoloration occurring frequently in paddy cultivation. Chemical property of soil and inorganic nutrient component of leaf were analyzed on abnormal fields of 7 regions where leaf discoloration occurred severely and normal fields of 7 regions among ginseng garden. The pH of abnormal fields was strong acidic condition (pH 5.51) compare to normal fields of slightly acid condition (pH 6.42). Calcium and magnesium content in abnormal fields were lower distinctly than that of normal fields, while EC, organic matter, phosphate, and potassium content showed not distinct difference between abnormal and normal fields. Whereas calcium and magnesium content were distinctly high in normal fields, both of potassium and iron content of ginseng leaf were distinctly high in abnormal fields. In particular, iron content of abnormal fields was more 1.94 times in soil, and 3.03 times in leaf than that of normal fields. In soil chemical property, there were significant negative correlation between leaf discoloration ratio and soil pH, and there were also significant positive correlation between leaf discoloration ratio and iron content. In ginseng leaf, there were highly significant negative correlation between leaf discoloration ratio and calcium content, and there were also highly significant positive correlation between leaf discoloration ratio and iron content.

인삼은 음지성 작물인데 7~8월 고온기에 강광과 고온하에서 잎의 가장자리부터 피해를 받아 결국 고사하게 되는 고온장해(엽소)가 발생한다. 엽소 피해를 받은 인삼은 근중의 감소, 뿌리 조직의 스폰지화로 인해 홍삼제조 시 내공의 발생율이 증가한다. 기존에 육성된 천풍은 지하부 체형과 홍삼품질이 우수하나 엽소에 약하다. 반면 엽소에 강한 연풍은 지하부 체형과 홍삼품질이 불량한 단점이 있다. 이에 따라 엽소에 강하고 홍삼품질이 우수한 인삼 품종의 개발이 시급하다. 본 연구는 엽소에 내성으로 선발된 ‘선일’ 품종의 시기별 고온장해 발생율, 엽록소 형광반응, Fm/Fv 값 변화, 인삼 잎 조직의 큐티클 층의 분포와 두께, 광합성 특성 및 유효성분 분석 결과를 보고 하고자 한다.

This study was carried out to investigate ginsenoside content in different root parts and the correlation between root diameter and ginsenoside composition of Panax ginseng C. A. Meyer cultivated by direct seeding. The unit contents of ginsenoside were 29.65, 28.76, 26.34 mg/g, respectively in 4, 5, 6 years old. However, the total contents of ginsenoside were 431.97, 606.56, 657.80 mg/root, respectively. Total ginsenoside content of fine root was higher than that of main root and lateral root. These tendencies were related to decrease by the increase of root diameter. When diameter of main root and lateral root were the same in different ages, the total ginsenoside content was higher in the order of 4 > 5 > 6 years old roots. Except for ginsenoside-Rg1, other ginsenosides components (PD/PT and total ginsenosides) had highly negative correlation with the root diameter within whole root, main root, lateral root and fine root, which indicated that ginsenoside content is correlated to root diameter. As results, it is suggested that ginsenoside content can be predicted.

Lime-bordeaux mixture has been used to prevent diseases in the field of ginseng. The purpose of this study is to investigate the efficacy of lime-bordeaux depending on the concentration and treatment time on major diseases of ginseng such as Alternaria blight and anthracnose, and to evaluate the root growth of ginseng. Lime-bordeaux caused damage on leaf when it was sprayed on ginseng between April and early May. No difference was found in root growth by spraying lime-bordeaux mixture between ratio 4-4 and 8-8 ratio in concentration. Plot of 6-6 raito and 8-8 ratio appeared to be similar efficacy compared to that of practical chemical control. However, the plot of 4-4 ratio showed lower than that of chemical control.