This study was carried out to know the active fraction of Dryopteris rhizoma on antimicrobial activity against some food spoilage microorganisms. Also, antimicrobal activities were investigated for the aqueous and ethanolic extracts of four herbs such as Terminaliae fructus, Eugeniae flos, Salviae miltiorrhizae radix and Dryopteris rhizoma. Antimicrobial activities of three herbs except for Terminaliae fructus showed higher activities in 75% ethanolic extracts than in aquoeus extract. Ethanolic extract of Dryopteris rhizoma showed the highest antimicrobial activity among extracts of four herbs. Antimicrobial activity intensities of solvent fractions of Dryopteris rhizoma extracted by 75% ethanol were order to CHCl₃ fraction$gt;EtOAc fraction $gt;BuOH fraction$gt;H₂O fraction. The CHCl_(3+) EtOAc and BuOH fractions also inhibited growth of food spoilage microorganisms as the concentration increased, respectively. In case of EtOAc fraction, 1000 ppm of fraction almost inhibited completely the growth of microorganisms tested.

This study was conducted to investigate the antimicrobial activity of grapefruit extracts and polylysine mixture against food-borne pathogens. The mixture was showed a potent and quick antibacterial activity for 5 major bacteria causing food poisoning i.e. Escherichia coli, Escherichia coli O-157, Salmonella typhi, Staphylococcus aureus, Vibrio cholerae. The antibacterial effect of the mixture on the ordinary bacteria inhibiting on the surface of lettuce was lasted even 6 hrs after the treatment, however the mixture was non-effective on the color, smell and taste of lettuce. The treatment with 10% mixture solution of the foods such as fish, meat, rice and bread suppressed the growth of the bacteria and kept the foods more freshly than the untreated foods.

To develop a lactic starter to produce antimicrobial substance for inhibiting the growth of a variety of foodborne spoilage bacteria in fermented foods, we investigated the antibacterial effect of the antibacterial substance, produced by Lactobacillus amylovorus IMC-1, against foodborne spoilage strains, and its sensitivity on the treatment of proteolytic enzymes. L. amylovorus IMC-1, which was isolated from a traditional cheese in Inner Mongolia, produced a maximum amount of antibacterial substance in the skim milk medium after 72 h incubation at 37℃, and further incubation resulted in the same activity. The substance obtained from gel filtration inhibited all strains used such as Bacillus subtilis IFO 3025, Staphylococcus aureus IAM 1011, Listeria monocytogenes VTU 206, Escherichia coli RB, and Pseudomonas fragi IFO 3458 at the concentration of 20 units/ml. This substance was found to show bactericidal action against B. subtilis, E. coli, and Ps. fragi, and bacteriostatic activity against both Staph. aureus and L. monocytogenes. The bactericidal action was due to cellular lysis. The substance is not organic acid, hydrogen peroxide and proteinaceous compound.

Antimicrobial activity of Korean leek was effective on Staphylococcus aureus, Bacillus cereus, Bacillus subtilis, E. coli O157:H7, Pseudomonas fluorescens, Pichia membranaefaciens. Extract from raw Korean leek and that from frozen one showed antimicrobial activity; in contrast, extract stored frozen condition or extract from blanched one lost antimicrobial activity. Filtration, centrifugation, and vacuum concentration did not affect antimicrobial activity of the leek, nor did the changes in storage pHs. Addition of ground leek to soy milk, soy curd, and broth for cold noodle resulted in the prolonged shelf life by significantly reducing the number of viable cells.

A survey was conducted to investigate the attitudes of the food specialist to the food developed by gene recombination. The mail survey was distributed to 1,400 food specialists and received 464, a response rate of 33.1%. Respondents were asked about knowledge, concerns of potential hazards, purchasing and labeling of the gene recombination foods. Most respondents (98.7%) have some knowledge on the gene recombination foods. 91.3% of respondents recognized necessity of gene recombination technology. However, they also point out its potential hazards (80.9%). The groups with less knowledge showed their increased worry on the hazard in comparison with ones of having more knowledge(p$lt;0.01). The result indicated that there was negative relationship between knowledge and worry on the gene recombination foods. The groups with more knowledge showed their increased purchasing on gene recombination foods in comparison with ones of having less knowledge(p$lt;0.01). The result indicated that there was positive relationship between their purchase intent for gene recombination foods and knowledge. 68.4% of respondents showed their interest on purchasing the gene recombination foods. In this group, most of them (44.9%) has on condition that low cost (27.0%). In addition, they also have not use the foods for their children (17.9%) if they buy it. Most respondents (85.3%) want labeling on the gene recombination foods.

The prevalence and serotype of food-borne pathogens was investigated from 888 samples of chilled meat, 222 samples of packed frozen meat and 117 samples of imported frozen meat during the period from March 1996 to October 1998. Isolation rates of pathogens associated with food poisoning were revealed in order of Staphyloccus aureus, Campylobacter jejuni/coli, Listeria monocytogenes and Salmonella spp, but Escherichia coli O157:H7 was not isolated in all of the meat samples. Amusingly, Cczmpylobacter jejuni/coli were isolated highly in refrigerated meat, but was not isolated in packed frozen meat. L. monocytogenes was encounted higher isolation frequency in packed frozen chicken meat than in refrigerated chicken meat. In the distribution of serotypes of isolates, most isolates of Sta. aureus classified as enterotoxin type C and D. All of the Salmonella spp. isolated from pork were diagnosed group A and most of isolates from chicken meat were grouped B and D. Most of L. monocytogenes isolated from chicken meat were grouped type 1 and a few number of isolates classified as type 4.

Recently, it is continuously rising to concern about the health risk being induced by microorganisms in food such as Escherichia coli O157:H7 and Listeria monocytogenes. Various organizations and regulatory agencies including U.S.EPA, U.S.DA and FAO/WHO are preparing the methodology building to apply microbial quantitative risk assessment to risk-based food safety program. Microbial risks are primarily the result of single exposure and its health impacts are immediate and serious. Therefore, the methodology of risk assessment differs from that of chemical risk assessment. Microbial quantitative risk assessment consists of four steps; hazard identification, exposure assessment, dose-response assessment and risk characterization. Hazard identification is accomplished by observing and defining the types of adverse health effects in humans associated with exposure to foodborne agents. Epidemiological evidence which links the various disease with the particular exposure route is an important component of this identification. Exposure assessment includes the quantification of microbial exposure regarding the dynamics of microbial growth in food processing, transport, packaging and specific time-temperature conditions at various points from animal production to consumption. Dose-response assessment is the process characterizing dose-response correlation between microbial exposure and disease incidence. Unlike chemical carcinogens, the dose-response assessment for microbial pathogens has not focused on animal models for extrapolation to humans. Risk characterization links the exposure assessment and dose-response assessment and involve uncertainty analysis. The methodology of microbial dose-response assessment is classified as nonthreshold and threshold approach. The nonthreshold model have assumption that one organism is capable of producing an infection if it arrives at an appropriate site and organism have independence. Recently, the Exponential, Beta-poission, Gompertz, and Gamma-weibull models are using as nonthreshold model. The Log-normal and Log-logistic models are using as threshold model. The threshold has the assumption that a toxicant is produce by interaction of organisms. In this study, it was reviewed detailed process including risk value using model parameter and microbial exposure dose. Also this study suggested model application methodology in field of exposure assessment using assumed food microbial data(NaCl, water activity, temperature, pH, etc.) and the commercially used Food MicroModel^ⓡ. We recognized that human volunteer data to the healthy man are preferred rather than epidemiological data for obtaining exact dose-response data. But, the foreign agencies are studying the characterization of correlation between human and animal. For the comparison of differences to the population sensitivity; it must be executed domestic study such as the establishment of dose-response data to the Korean volunteer by each microbial and microbial exposure assessment in food.

The present study was conducted to investigate the relation of eating behavior and food intake to 534 adolescents' obesity indices. Group means of relative body weight (RBW) were 93.66 for males and 97.12 for females. Body mass index(BMI) were 20.39 for males and 20.41 for females. Respondents were divided into 3 groups on the basis of RBW as an obesity index. Astonishingly, 44.76% of total respondents were underweight and 12.92% overweight and obese. 64.54% of respondents usually skipped their breakfasts and 24.23% their dinners. More males did not have their breakfasts than females. In contrast, more females skipped their dinners than males. A significant difference was found between males and females in their eating habits(p<.001) that should be changed. As their undesirable eating habits, males pointed out 'skipping meals', while females stressed 'overeating'. There were more overeating respondents in the overweight group, while more respondents skipping their meals were found in the underweight group. Many females(72.79%) and males(52.78%) tended to alternate consistently overeating and fasting to lose weight. The most important reason for losing weight was to improve their appearance(61.12%). In addition, nutrient score indices of respondents were very low with a mean of 33.39. Only 42.13% of total respondents showed good nutrient score indices. More males ate meat, bean, white vegetables, potato and fat than females(p<.05), whereas more females ate bread, rice cake and fruit than males(p<.01). Among the 3 groups -overweight, normal weight and underweight groups, overweight group was the most eaters of meat, fish, egg and milk; underweight group was the least eaters of them.

The health, anthropometry, food habits, food intake frequency and nutrition intake of 747(219 men and 528 women) elderly people living in Inchon were investigated. Mean BMI, WHR, triceps skinfold thickness, BIA, blood pressure of the subjects were 23.4, 1.0, 15.6mm, 28.6%, 137.5/78.8mmHg in men and 24.9, 0.87, 22.6mm, 36.2%, 130.8/73.8mmHg in women respectively. Over the half of the subjects perceived themselves unhealthy and 86.2% of the elderly suffered from one or more illness. Ratios of the smoking and the drinking elderly were 40%, 43% in men and 12%, 11% in women. The major reason to stop smoking or drinking was the concern about their health. Food habit score of the subjects was relatively high (14.45/18.0) but food intake frequency score were relatively low (21.18/33.0) except the food groups of cereal & grain, white vegetable and sweets. The foods consumed by the elderly were very limited in quality and also in quantity. The daily intakes of calorie, protein, iron, vit. B1, niacin and vit. C were less than 2/3 of RDA and the amounts of calcium, vit. A and vit. B2 consumption were less than 1/2 of RDA.

본 연구는 우리나라 가계의 식생활비 지출행태에 관한 이해를 꾀하고자 식료품비와 외식비로 나뉘어 각각에 관한 결정요인을 비교, 분석하였다. 연구를 위하여 통계청에서 조사한 1994년 도시가계연보의 원자료를 사용하였으며, 식료품비와 외식비 지출에 대한 관련요인들의 영향력을 고찰하기 위하여 다중회귀분석이 사용되었다. 연구결과에 의하면 식료품비와 외식비 지출 모두 가계소득과 정적 상관관계를 보였으며, 주부가 취업한 경우 식료품비 지출은 비취업주부가계에 비해 적은 반면, 외식비 지출은 도리어 많은 것으로 분석되었다. 가구원수, 교육수준, 계절, 자녀의 존재 변수 등이 식료품과 외식비지출에 대해 유의한 영향력을 미치는 것으로 나타났다. 가구주 나이는 식료품비에 유의한 영향을 미쳤으며, 가구주 직업은 외식비지출에 영향을 미치는 것으로 분석되었다.

The status of general food service administration and nutrition education was investigated in 111 elementary schools located in Inchon Metropolitan Area by questionnaires. The results are as follows ; 1. Most schools served 5 lunches per week. Meal cost per capita was 1212.1 won in urban type school and 979.0 won in rural type school. Most schools(91.9%) had 1 regular dietitian and 1 regular cook. With increasing number of students the number of temporary employees increased significantly. Most school dietitians(86.5%) were in their twenties and had 2 year careers. 2. Most dietitians(90.1%) planned the menu based on the RDA with the first consideration in nutritional needs of the students. Many dietitians(81.8%) surveyed on the food preferences of the students at least once a year. About 86% of the schools had the food service committee which was helpful. Dietitians were the first one in charge of food purchasing and checking, and sometimes a manager in general school affairs worked together. One third of the schools used the volunteer parents in food services. 3. Most school dietitians(97.2%) gave the students nutrition education using handouts once a week. However, they could not teach the students through the classroom lecture because they are not the regular teachers. Food garbages were removed by the professional trash collecting company or by using fermentation machines or by giving them to animals as feeds. School events concerning the food and nutrition were mostly drawing pictures and composition. Only 36.4% of the school dietitians had experienced in nutrition counseling for an individual student. Most of them(99.1%) thought that individual counseling is necessary if they have enough time and chances to do.

본 논문은 한일 양국의 가계에 있어서의 식품수요에 미치는 요인을 파악하기 위해서 종래의 수요모델에 코호트(cohort, 행동을 같이 한 집합체) 분석적인 생각을 도입하여 한일 양국의 식품수요 형태분석을 하였다. 여기에 제시한 새로운 수요분석 모델은 소비지출과 가격이 식료수요에 미치는 경제적 효과 이외에 세대주 연령효과나 출생연도와 같은 비경제적효과 등도 계량한 것이 특징이다. 분석한 결과를 요약하면 다음과 같다. 1. 한일 양국의 식품수요의 품목군별 탄력성을 보면 유지류, 음료, 조리식품의 수요탄력성은 한국보다 일본이 더 탄력적이었지만, 다른 모든 품목은 일본보다 한국이 탄력적이었다. 곡류, 육류, 외식의 외부화 식품의 소비지출과 가격탄력성은 한국의 큰 품목과 일본의 큰 품목이 서로 상충하고 있어서 일정한 경향 파악이 곤란하다. 그러나 신선식품에 대한 소비지출과 가격의 탄력성은 모두 일본보다 한국이 크다. 2. 식품에 대한 지출액은 세대 구성원의 연령을 반영하여 비교하여 볼 때 한국과 일본의 결과가 비슷하였다. 즉 세대주 연령이 젊은 계층의 가족에는 유아가 있기 때문에 유란류의 지출액이, 또한 중년층에서는 청소년이 많기 때문에 과자류의 지출액이 다른 연령계층에 비하여 많았다. 한국은 연령계층이 높을수록 2세대 가족이 많기 때문에 다수 품목에 지출액이 많고, 일본의 중년층은 과자류를 포함하여 특히 곡류, 육류 등의 에너지 식품이나 외식의 지출액이 많다. 그러나 연령이 높은 계층에서는 세대 구성원이 거의 성인이며 평균연령이 높기 때문에 곡류, 육류, 외식 등의 지출액이 적었지만 어패류, 야채류 등의 전통식품이나 조리식품의 지출이 많았다. 3. 식품소비의 패턴은 세대주 출생연도별로 비교하면 한일 양국 모두 구세대일수록 주식 중시의 경향을 나타내고 신세대일수록 축산물, 유지류, 외부화 식품의 비중이 높았다. 그러나 품목 구성의 세대간 차이는 한국에 있어서는 매우 크지만 일본의 경우는 비교적 작았다. 따라서 식생활의 서구화, 외부화는 세대교체에 동반하여 한국에는 급속히 진행하지만 일본은 점진적으로 진행한다고 볼 수 있다. 4. 가계 식품 수요의 장기 변화에 미치는 요인은 소비지출, 가격, 세대주 출생연도, 연령 등의 네 가지 요인으로 나누어서 각각의 효과를 요인간으로 비교하면 한일 양국이 함께 가격의 효과가 가장 작다. 그러나 그 이외 요인별 효과의 상대적 중요성은 양국간에 다른데 한국은 소비지출의 효과가 출생연도나 연령 효과보다 크지만 일본은 경제적 요인인 소비지출보다 세대주의 출생연도나 연령 등의 비경제적 요인의 효과가 크다.

This study investigates the impact of International Monetary Fund(IMF) Age on the household behavior of food consumption and analysis the related factors(demographic variables, family life style) to find out the reasonable consumer's consciousness and food market conditions. The results are as follows. 1. General life style and food life style, considered as family life style, were classified into 7 types and 9 types respectively as family life style by factor analysis. 2. The rationality of using and disposing stage on food life has increased, the rationality of the planning stage on food life has decreased since the IMF age began. But the rationality of overall food consumption has hardly changed. 3. The amount of purchasing was decreased, discount store was favorite place to buy food, and safety and freshness were major determinants in purchasing for most of food items. But this was different with food items. The various information sources were used impartially to buy food, cash was used mainly, and the degree of using the discount ticket/coupon was a little low. 4. Income level is an influential factor on the rationality of the planning and purchasing stage on food life, while environment-oriented food life style has influenced on the rationality of using and disposing stage. The purchasing amount of grain products, meats, milk products, bread and snacks, drinks, alcoholic drinks, water and convenience goods has influenced by demographic variables, eating out had been influenced by general life styles, and fruits, healthy foods, processed foodstuffs, favorite foods, vegetables seaweeds, import foods and seafood had been mainly influenced by food life style since IMF age began.

A change in distribution structure affect a consumption structure which may result in considerable changes in lifestyle including food purchase. The purpose of this study was to investigate the effect of change of distribution structure on the indigenous fermented food purchase. The informations of this study were obtained from 407 housewives residing in Seoul and the capital area by using questionnaire during May to June, 1997 Their data were statistically analyzed based on frequency, mean, and 2-test. The results were as follows; 1) The percentages of subjects buying ready made fermented food were 69.8% for Jotkals(salt-fermented fish products), 22.3% for Kochujang(fermented red pepper soybean paste), 21.6% for Janachis(pickled basic side dishes), 21% for Kanjang(Korean style soybean sauce), 17.1% for Toenjang(Korean style soybean paste), and 3% for Kimchi(seasoned and fermented vegetables). 2) With decreasing age, preparation ability of Kanjang, Janachis, and Kimchi. The subjects living close to the new distribution channel were higher in ready made fermented food than the others. 3) Compared to the subject living far from the new distribution channel, the subject living close to the new distribution channel was high in frequency of new distribution channel utilization for indigenous fermented food purchase but low in frequency of conventional market utilization. The younger in age, higher in educational level, and nuclear family, the higher the utilization of new distribution channel for indigenous fermented food purchase.

대구지역 남자 대학생의 체중조절지향 및 체형에 따른 식습관 및 기호도, 영양지식을 조사하기 위하여 대구 C대 남학생 219명을 대상으로 설문지 및 신체계측을 통하여 조사한 결과는 다음과 같다. 1. 조사대상자의 평균연령은 19.8±2.3세였으며, 평균 신장 및 체중은 173.8±8.7cm 와 65.8±7.9Kg, 그리고 평균 BMI는 21.9±2.4로 한국인 체위기준치의 표준신장 172.0cm, 체중 66Kg, 평균 BMI 22.3과 비슷한 수준이었다. 조사대상자들이 생각하는 이상적인 신장과 체중은 179.3cm와 70.4Kg로 이는 대체로 실제보다 큰 체형이었으며 이때 BMI는 21.9였다. 2. 스스로 평가한 자신의 체형은 '보통이다' 혹은 '여위었다'라고 생각하는 대상자는 72.7%였으며. 이상적으로 생각하는 체형은 '약간 살찜'이라고 답하였다. 체중조절에 대한 관심은 '좀 더 살찌기를 원함'이라고 답한 대상자가 74명으로 전체의 33.8%였고, '여위기를 바람'이라고 답한 대상자는 47명으로 전체의 21.5%였으며, '지금의 상태에 만족함'이라고 답한 대상자는 98명으로 44.7%였다. 3. 평소 식사량은 많은 편으로 '먹을 수 있는 최대의 양을 먹는다'고 답한 수는 전체 조사대상자의 40.6%였으며 체중군별로는 과체중군이 48%로 가장 높았다. 각 체중군이 가장 중점을 두고 식사를 하는 시간은 '저녁식사'였다. 식사의 규칙성은 전체 조사대상자의 51.6%가 '아침'을 규칙적으로 먹는다고 답하였으며 저체중군은 점심을 불규칙적으로 하는 것으로 조사되었다(51.1%). 식사를 거르는 이유는 '시간이 부족해서'라고 전체 조사대상자의 60.3%가 답하였으며 과체중군에서 '습관적으로 식사를 거른다'가 다른 군보다 높게 나타났다. 자신의 식생활에서 가장 문제가 되는 것은 '불규칙한 식사시간'이라고 조사 대상자의 45.2%가 답하였다. 4. 영양관련 정보를 습득하는 경로는 조사 대상자의 49.8%가 '신문이나 잡지, TV'라고 답하였으며 '부모님', '학교수업' 및 '영양관련 서적' 등 이었다. 반면에 '영양관련 지식의 습득이 전혀 없다'라고 대답한 조사대상자도 26.5%나 되었다. 식품군별 섭취빈도조사 결과 '저체중군'은 '육류'의 섭취빈도가 높은 반면 '과체중군'은 '채소'의 섭취빈도가 높게 나타났다. 5. 영양관련 지식에 대해서는 각 체중군은 20점 만점에 평균 15점을 기록하였다. 이들 체중군 사이에는 유의적인 차이가 없었으며 조사 대상사의 연령이 증가함에 따라 영양관련 지식은 상대적으로 감소하였다(p〈0.05). 6. 체형에 따른 식품섭취빈도는 일반적으로 '곡류 및 면류'를 가장 선호하였으며, '야채류 와 '기호식품 및 간식류'의 선호도도 높은 편이었고 '햄, 소세지'에 대한 선호도는 모든 체중군에서 낮게 나타났다. '김치'의 섭취빈도가 각 체형군에서 고르게 가장 자주 섭취하였다. '과체중군'은 다른 군에 비해 '쇠고기'와 '기호식품 및 간식류'의 섭취빈도가 낮아 유의적인 차이를 보였다(p〈0.01). 저체중군'은 '과체중군'에 비해 육류의 섭취빈도가 높은 반면 '과체중군'은 야채와 과일 섭취빈도가 높았다. 이상의 결과로 남학생의 체형에 대한 올바른 인식과 이에 따른 식사행동이나 식습관의 바람직한 방향을 위하여 영양학의 이해나 영양교육이 필요할 것으로 사료된다.

This study was undertaken to assess eating out behavior and factors in food choices of 369 college students in Kwangju. 47.1% of subjects ate out at least once a day and lunch was the most frequent meal for eating out. Eating out expenditure was significantly associated with student's allowances. Significant differences were observed in frequency, expenditure of eating out and related factors in food preference between male and female students. Taste was the most considered factor but nutrition was the least considered in food selection of respondents. Among male respondents, the most important nutritive factor was protein followed by calorie and vitamin. However, female counterpart regarded calorie as the most important nutritive factor affecting food selection. Tendency of increased vegetable and fruit consumption was noted in subjects over past a few years. It might be suggested that effective nutrition education for college students be implemented to improve and develop eating out behavior related to nutrition in food selection.

The study was carried out from March to May in 1998 to compare the food habits and food preference of 391 men in Kyung Nam area who were between twenties and sixties. The summarized results are as follow: 1. The 30's were highest(173.8cm) and the 60's were lowest(168.3cm) in their average stature. The 30's were heaviest(68.2Kg) and the 20's were lightest(62.3Kg) in their average weight(P<0.001). Average BMI was highest in the 50's and lowest in the 20's. 2. Average food habit score of the subjects(4.08) was as low as belonging to the poor group and lowest was 20's. 3. The rate of not eating was highest in the breakfast and the subjects ate breakfast more as they were older(P<0.001). The serious problem of food habit was irregular eating time. The subjects ate in the more irregular time as they were younger. 4. 88.9% of the subjects preferred cooked rice and cereals as their main food, kimchi as their side dish and fruit as their eating between meals. 5. We found great differences in the kinds and number of drinkings subjects preferred as their ages(P<0.001). 20's preferred cider and cola. 30's, 40's and 50's preferred coffee and 60's preferred ginseng tea and citron tea. The rate of smoking was higher in 30's and 40's then in 20's and 50's. 6. 72.9% of the 20's and 16.7% of the 60's ate instant food more than 2-3 times a week. They ate Ramen most often because of its convenience. They ate out more as they were younger and preferred Korean food as their eating out menu. 7. The degree of preference in a sweet taste was highest in 20's and lowest in 40's (P<0.01) and the degree in the preference of a sour taste became lower as they were older(P<0.001). Only in the preference of a salty taste, we could find the difference of the BMI level. They showed high degree in the preference of a salty taste as the order of the over-weight group, normal group, under-weight group and fat group. They showed meaningful correlationship between the level of BMI and the degree of preference of a salty taste in 20's and 40's and between the food habit score and the level of BMI in 60's (P<0.05).

Safety of present food has been accepted on the basis of extensive use and experience for a long time. Many food resources have been developed by traditional techniques without any significant adverse impacts on the safety of food. Recently recombinant DNA techniques are being used to develop new food resources. These techniques enable developers to make specific genetic modifications in food resources that introduce substances that could not be introduced by traditional methods. With these techniques food resources are being to resist pests and disease, to tolerate herbicides, and to have improved characteristics for food preservation and nutritional contents. Because the properties of an organism result from interaction between biochemical pathways controlled by many genes, the genes conferring these traits usually encode directly responsible proteins for the new trait as well as proteins that indirectly modify carbohydrates or lipids in food. Therefore, this kind of food is regarded as new food that has not been existed before, and the safety of the food developed by recombinant DNA techniques should be evaluated upon scientific basis. In this paper, the issues upon safety of the food developed by gene manipulation are discussed in terms of composional changes that can be introduced, potential food safety harzards that might arise, present status of safety regulations in various countries and international organizations, and suggestions for the safety regulation in Korea.