윌리엄 블레이크는 􋺷네 생물􋺸에서 네 생물과 방사물이 이원론적 대립과 갈등을 일으킨 후에 조화와 재창조를 성취하는 길을 제시한다. 네 생물과 방사물은 인 간 내면의 분열과 고통, 남성과 여성의 성적 갈등, 이성과 감정의 갈등, 이성적 질서와 창조적 힘의 대립, 생명과 죽음의 갈등, 상상력과 질서의 대립, 혁명과 파괴와 질서 사 이의 대립 등을 역동적으로 보여준다. 그 후에 이들은 최후의 심판에서 조화와 재창조 를 시도하여 새로운 인간세계를 구축한다. 􋺷네 생물􋺸에서 이러한 블레이크의 상상력은 현대인들에게 상상력의 중요성을 각인시키고, 새로운 세계에 대한 비전을 제시한다.

The purpose of this study was to analyze English subject-specific descriptions through text mining, examine their alignment to the curriculum achievement standards, and ultimately support students’ educational growth. We collected English subject-specific descriptions for 1,030 tenth-graders from general high schools in City A, and keywords and contexts were extracted using TF, TF-IDF, phi coefficient analysis, and trigram analysis. The results revealed that the subject-specific descriptions frequently neglected the following aspects of learning and instruction in curriculum achievement standards: familiar and general themes, conversation and listening skills, language use across different types of information, and proper email writing. Although descriptions are intended to reflect students’ actual achievement levels, those for lower achievement levels (i.e., D and E) were often overstated. Improvements are necessary to ensure closer alignment between the descriptions and the curriculum achievement standards, with particular emphasis on the accurate representation of lower achievement levels. Based on the findings, we discussed potential improvement strategies for educational authorities to consider and implement.

The Grifola frondosa cultivar KMCC03118 was used to isolate monokaryotic strains via spore separation, resulting in the successful crossbreeding of strains KMCC03118-11 and KMCC03118-23, which produced F1 hybrids. These F1 hybrids were then further crossed with various monokaryotic strains to generate F2 progeny. In evaluating the effects of different medium compositions on fruit body development, it was found that a substrate consisting of wheat bran and dried sawdust, with a carbon-to-nitrogen (C/N) ratio of 66-68, provided the most favorable conditions for mycelial growth. Among the strains tested, KMCC03137 and GF-18-50 demonstrated superior characteristics, including a larger fruit body diameter, thicker pileus, and greater stipe thickness, with the highest productivity observed at 143.6 ± 13.3 g and 144.7 ± 15.2 g, respectively. Furthermore, the color of the caps (L: 29.7 ± 7.1, a: 2.6 ± 0.7, b: 8.2 ± 1.8) remained consistent, indicating stable high-quality production. Based on these results, the optimal substrate composition for enhancing both the quality and productivity of the fruit bodies was determined to be 42% Quercus sp. sawdust, 42% Quercus sp. fermented sawdust, 6% wheat bran, and 10% dried tofu residue. This study provides a crucial foundation for the commercial cultivation and breeding improvement of Grifola frondosa, offering valuable insights into its genetic enhancement, and providing essential data for future research aimed at increasing the species' genetic diversity and productivity.

The Japanese encephalitis virus (JEV) is a zoonotic pathogen that affects the nervous systems of humans, pigs, and horses. It has been classified into five genotypes (G1-G5) based on molecular analysis of the pre-membrane or envelope gene. In the Republic of Korea, the predominant JEV genotype has recently shifted from G3 to G1 and G5, highlighting the need for a rapid and accurate diagnostic method. In this study, we designed specific common and differential primer sets for JEV G1, G3, and G5 to detect the JEV gene. Four specific primer sets for JEV G1, G3, and G5 were used to selectively amplify the target gene. The detection limits of the common primer set for JEV G1, G3, and G5 were 100, 0.1, and 10 TCID50/reaction, respectively. The detection limits of the three differential primer sets were 1, 0.1, and 1 TCID50/reaction, respectively. No cross-reactivity was observed with non-JEV reference viruses. We successfully developed a multiplex reverse transcription polymerase chain reaction (RT-PCR) assay to distinguish the three JEV genotypes. Our multiplex RT-PCR assay is highly sensitive and specific, providing a reliable tool for confirming JEV infection in suspected samples. Additionally, our assay can be applied to suspected mosquito samples and commercial veterinary biological products.

Biomimetic study is being conducted in various fields and applied to the development of technology for the realization of a sustainable society. In this study, we analyzed the cuticular surface structure and wax layer composition of the leaves of Myriophyllum verticillatum and Azolla imbricata to investigate the antifouling characteristics. Field emission scanning electron microscopy analysis (FE-SEM) and contact angle measurement revealed that the surface of M. verticillatum had an irregular and curved layered structure with non-directional filament structures and showed high hydrophobicity. On the leaves of A. imbricata, amphiphilic structures with nano-sized hydrophobic plate-like filament structures and micro-sized irregular hydrophilic spikes were observed. The dorsal surface of the A. imbricata leaves had a denser distribution of hydrophobic nano-structures compared to the ventral surface. The dorsal surface of the leaf showed high hydrophobicity, while the ventral surface showed wettability. Due to the habitat characteristics of A. imbricata, which is a floating plant, the ventral surface is constantly in contact with water, while the front surface has a hydrophobic surface. Analysis of the wax composition of plants revealed that M. verticillatum and A. imbricata were mainly composed of saturated fatty acids, ketones (2-Nonadecanone and 2-Heptadecanon), and sugar alcohols such as 1,30-Triacontanediol. These substances have high antimicrobial activity, and saturated fatty acids form stable and rough anisotropic crystalline surfaces. The hierarchical amphiphilic structure and the non-toxic stable hydrophobic surface observed in the cuticle of aquatic plants are expected to be utilized as materials for the development of sustainable antifouling technologies.

This study aimed to address the time, cost, and ethical issues associated with traditional animal experiment-based observational methods by utilizing in silico Physiologically Based Pharmacokinetic modeling to predict veterinary drug residues in livestock products and validate them against observational data. Using PK-sim software, we modeled the physiological conditions of pigs to predict the depletion of ceftiofur and spiramycin. We evaluated the ceftiofur (3 mg, 6 mg) and spiramycin models by comparing them with observational data using residuals, MSE, and R-squared values. Specifically, the R-squared values for the ceftiofur models were all negative, indicating poor predictive power. For Ceftiofur (3 mg), the R-squared value was <0 with MSE of 611.3764, and for Ceftiofur (6 mg), it was <0 with MSE of 2447.982, highlighting significant discrepancies. Similar shortcomings were observed in the spiramycin models, with an R-squared value of <0 . These discrepancies can be attributed to inaccuracies in literature data, limited physicochemical data, inadequate consideration of inter-individual differences, mismatches between experimental and model conditions, and limitations of benchmark observational experiments. This underscores the critical importance of enhancing data quality and refining modeling approaches. Future research should focus on validating in silico techniques across diverse animal models and drugs to broaden their applicability in safety assessments. Ultimately, leveraging in silico techniques is crucial for establishing a scientifically robust safety management system for livestock products, overcoming the constraints of current observational experimental methods.

This study evaluated the quality characteristics of Flammulina velutipesduring storage using modified atmosphere films of different thicknesses (20, 40, and 60 μm). The films included high-density polyethylene (HDPE) and low-density polyethylene (LDPE). F. velutipeswere stored at 1°C for six weeks, and quality was assessed based on weight loss, respiration rate, firmness, color parameters, β-glucan content, total phenolic content (TPC), and antioxidant activities (2,2-diphenyl-1- picrylhydrazyl and 2,2'-azino-bis [3-ethylbenzothiazoline-6-sulfonic acid] radical scavenging activities). All HDPE and LDPE films were more effective than the conventional film (polypropylene) at maintaining mushroom quality, particularly in the later stages of storage. In particular, LDPE films with thicknesses of 20 and 40 μm showed superior performance at reducing respiration rates and weight loss, while mushrooms packaged with these films retained higher TPC and antioxidant activities. The β-glucan content also remained more stable in mushrooms stored using HDPE and LDPE films. Although we did not evaluate changes in sensory properties or nutritional components, such as vitamins, our results suggest that the type and thickness of packaging films significantly influence the preservation of the quality of F. velutipesduring storage. Additionally, LDPE films with thicknesses of 20 and 40 μm were found to be the most suitable packaging materials for the distribution and storage of F. velutipes. Furthermore, these findings are expected to provide valuable insights into the selection of optimal packaging materials to extend the shelf life and maintain freshness during the postharvest handlingof F. velutipes.

In this study, Pleurotus ostreatus were grown in bottles at temperatures set to 15°C, 20°C, and 25°C inside the cultivation room. Changes in temperature, CO2 concentration, and humidity inside the bottles were measured, and growth characteristics according to the temperature conditions were evaluated. CO2 concentration increased overall as the temperature increased and was particularly stable at 20°C, suggesting that 20°C is the optimal condition for the physiological respiration of P. ostreatus . While humidity was relatively constant at 15°C, it decreased over time at 20°C and was maintained at a stable level at 25°C, suggesting that water retention capacity may occur at high temperatures. As a result of the growth investigation, the yield per bottle and individual weight were the highest at 20°C, confirming that 20°C is the most suitable temperature condition for the growth of oyster mushrooms. At 25°C, the yield per bottle was maintained but the individual weight decreased and the color tended to change. These results suggest that the interaction between CO2 concentration, humidity, and temperature has a significant effect on the growth and quality of oyster mushrooms, and that it is effective to control the cultivation room temperature to 20°C for optimal growth.

Lactobacillus is a probiotics that improves various diseases and enhances immunity through the activation of cytokines involved in natural immunity. A variety of studies on immunoregulation have been conducted on various Lactobacillus, but few on Lactobacillus johnsonii (Lj) and especially on immune cells. In this study, we investigated how Lj affects the viability and function of RAW264.7 macrophages. The results indicated that Lj decreased the metabolic activity of RAW 264.7 cells and caused a decrease in the number of cells. In Annexin V and PI staining, the viable cells was significantly decreased at 107 cfu/mL Lj concentration. Measurement of cytokines using enzyme-linked immunosorbent assay (ELISA) showed that Lj increased the production of tumor necrosis factor (TNF)-alpha. Also, Lj increased the expression of CD40 and CD86, confirmed by flow cytometry analysis. Taken together, Lj caused decreased metabolic activity and cell viability, but simultaneously increased the expression of macrophage activation markers and TNF-alpha production. Therefore, when an appropriate dosage of Lj is administered, it is expected that the necessary immunomodulation can be increased while minimizing damage on cells. This study provided evidences for the immunomodulatory effects of Lj on RAW 264.7 cells in vitro and is expected to be a valuable information for Lj research.

This study compared and analyzed fish communities and mortality rates based on different survey methods (mesh size and retrieval frequency) of stationary nets, which are actively used in freshwater fish surveys in Korea, and proposed an appropriate survey method. Field surveys were conducted on the mainstream of the Nakdong River from May to June 2022 through a total of 10 times of fyke net surveys. The number of species and individuals collected varied significantly depending on the mesh size of the net (5 mm vs. 15 mm) (P<0.001), and larger species with an average total length of over 9 cm were mainly collected in the 15 mm mesh. However, the number of species (P=0.684) and individuals (P=0.100) collected did not show statistically significant differences depending on retrieval frequency (once/24 hours vs. twice/24 hours). In contrast, the mortality rate of fish differed based on all survey methods (mesh size, P<0.001; retrieval frequency, P<0.05), with mortality decreasing as the mesh size increased and retrieval frequency increased. Fyke nets are one of the standard fishing gears used in freshwater fish research in Korea, and to ensure species diversity, it is recommended to maintain the current standard mesh size of 5 mm. However, reducing the current 48-hour deployment time or increasing the retrieval frequency could lower the mortality rate. Furthermore, future research should focus on the differences in fish communities based on the combination of fishing gears commonly used in Korea to improve fish survey methodologies suitable for domestic freshwater environments.

Ganoderma lucidum, a fungus of significant scientific interest that encompasses a diverse array of bioactive compounds, has been extensively investigated for its potential health-promoting and disease-preventing properties. Ganodermanontriol, a triterpenoid, is the principal active component contributing to its biological activities. This study aimed to explore the anti-inflammatory properties of ganodermanontriol and to evaluate its potential as a functional ingredient. The expression of inflammatory mediators, including tumor necrosis factor (TNF)-α and inducible nitric oxide synthase (iNOS), and nitric oxide (NO) production was significantly inhibited in ganodermanontriol (1.25–5 μg/mL)-treated compared to that of untreated lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Additionally, the phosphorylation of mitogen-activated protein kinases (MAPK), specifically p38 and c-Jun N-terminal kinase (JNK), was markedly inhibited in ganodermanontriol (3–5 μg/mL)-treated cells. These findings indicate that ganodermanontriol possesses significant potential as both a prophylactic and therapeutic agent for immune-related disorders, owing to its ability to modulate inflammatory responses.

본 연구는 가톨릭 민족주의와 프로테스탄트 합병주의라는 이분법적 환 원주의를 강화시키는 아일랜드의 기념 정치를 탐구하기 위해 마이클 롱리가 사용한 기억 시학을 분석한다. 첫째, 롱리의 기억 시학은 양귀비꽃 이미지를 사용하여 제1차 세계대전과 북아일랜드 분쟁을 상징적으로 연결시키며, 기념 정치의 이분법을 극복한 다. 둘째, 롱리는 포스트-1916세대의 노스탤지아를 통해 역사적 희생자들 간 연대의 비전을 제시한다. 그는 과거에 실현되지 않은 가능성들을 탐구하는데 있어 세계대전과 북아일랜드 분쟁의 희생자들을 병치시켜 당대 지배적인 기념 정치를 탈신화한다.

This study investigated the relationship between teacher support in the areas of learner autonomy, relatedness, and competence, and motivational beliefs regarding achievement goal orientations and perceived task value in Chinese EFL college classrooms based on self-determination theory. A questionnaire was administered to 712 college students, and the data were analyzed using SPSS 26.0 and AMOS 24.0. The findings indicated that teacher support for autonomy, relatedness, and competence was positively correlated with mastery goal orientation and performance-approach orientation, while it was negatively correlated with performance-avoidance orientation. Furthermore, these forms of teacher support positively influenced students’ perceived task value. Achievement goal orientation mediated the relationship between the three types of teacher support and student engagement. However, perceived task value only mediated the relationship between teacher autonomy support and student engagement. This study offers valuable insights for enhancing English teaching practices by promoting student motivation through addressing their psychological needs in EFL college classrooms.

This study explores the introduction and applicability of the Species Protection Index (SPI) as a tool to evaluate the effectiveness of biodiversity conservation. Specifically, the SPI was recalculated for amphibians in Korea and compared with the internationally provided SPI results. The pilot evaluation of SPI for amphibians showed an increase from 41.52, based on international data, to 44.25, indicating that SPI calculations using domestic data can reflect conservation status more accurately than international SPI results. The findings suggest that SPI can serve as an important scientific basis for formulating national biodiversity conservation policies and managing protected areas, contributing to the development of more effective conservation strategies.

To prevent and improve various metabolic-related diseases caused by modern high-energy eating habits, alternative meats using mushroom materials are being researched. In this study, high-moisture (HMMA) and low-moisture meat analog (LMMA) were prepared using Pleurotus ostreatus fruiting body (oyster mushroom) powder and isolated soy protein as the raw materials in a co-rotating twin-screw extruder. Textural characteristics tended to decrease as the oyster mushroom content increased. HMMA exhibited a fibrous structure similar to that of chicken, whereas LMMA did not show a characteristic fibrous structure. The water absorption capacity of substitute meat decreased with increasing mushroom powder content. Radical scavenging activity, a measure of antioxidant activity, increased with increasing mushroom content in the substitute meat because of the influence of antioxidant components such as polyphenols in mushrooms. In terms of the prepared substitute meat's color, it was less vibrant and lacked intensity, which is thought to make it less appealing to customers. To address this issue, more ingredients need to be investigated.

This study investigates the effect of machine translation (MT) use on the writing performance of Korean EFL students, focusing on complexity, accuracy, and fluency (CAF). Six participants completed a series of writing tasks in which they first translated their L1 writing into L2 manually and then used MT to revise their L2 drafts. This process was repeated across ten different writing topics. Participants’ drafts were analyzed using CAF measures to assess MT’s impact on their writing performance and observe changes over tasks. The results show that MT significantly improved accuracy and fluency. However, gains in syntactic and lexical complexity were less evident. While group-level analysis showed consistent progress, individual trajectories varied widely, indicating diverse patterns of development. Overall, the findings suggest that MT enhances writing accuracy and fluency among Korean EFL students, although its impact on syntactic and lexical complexity is limited. These results indicate that MT can serve as a valuable tool for self-directed learning, helping students independently improve their writing accuracy and fluency and develop essential self-editing skills. This study highlights the potential of MT as a supplementary tool to support EFL students’ writing development, along with traditional instruction.

Processing of medicinal plants and mushrooms plays a crucial role in oriental medicine. This study investigated the antioxidant potency and chemical composition of antler-shaped Ganoderma lucidumprocessed under different heating temperatures (120 to 180 °C) and times (10 to 20 minutes). Processed samples were analyzed for their antioxidant activities, including total phenol content, DPPH, ABTS radical scavenging capacity, NO assay, ROS scavenging capacity, and chemical composition changes. Antioxidant potency was highest with processing at 180 °C for 10 min and at 210 °C for 10 min. This enhanced activity is likely attributed to changes in compound functional groups due to heat processing, which in turn influences antioxidant activity. In contrast to antioxidant potency, chemical components, including ganoderic acid D, ganodermanonitriol, and ergosterol, showed decreasing trends with increasing temperature and time.

본 논문은 윌리엄 버틀러 예이츠(W. B. Yeats)의 시 나는 그대의 주 (“Ego Dominus Tuus”)에 나타난 니체적 예술관과 자아 이론을 분석한다. 예이츠는 니 체의 사상을 긍정하며 자아와 반자아의 갈등을 창조적 에너지로 전환하고, 이를 통해 예술적 자아와 이상적 삶의 가능성을 탐구한다. 본 논문은 예이츠가 마스크 이론(Mask theory)을 통해 자아를 재구성하고자 한 의도를 조명하며, 자아의 분열과 대립을 예술 적 이상화 과정의 필수적 요소로 이해했음을 논의한다. 또한, 나는 그대의 주 에 나타 난 예이츠의 창조적 긴장과 자아의 변형 과정을 통해, 그의 시 세계가 지향하는 이상 적 삶과 예술의 상호작용을 규명한다. 이를 통해 예이츠가 니체의 예술로서의 삶을 어 떻게 자신의 시적 비전에 통합했는지를 밝힌다.

The genus Xylaria, a large and diverse group within Ascomycota, plays an important role in forest ecology as decomposers. Several new Xylaria species have been recently identified. In this study, the morphological and molecular characteristics of Xylaria species found in Korea were analyzed based on the latest classification systems. Notably, species with characteristics similar to Xylaria cubensis and X. rogersii, which were not previously reported in Korea, were identified. X. cubensis grows on rotten wood and is characterized by brownish clavate stromata with rounded apices and small, dark ascospores. X. rogersii, found in Magnolia spp., has long stromata with a dark-brown, fine-striped outermost layer and subhyaline to yellowish single-celled ascospores. Genetic analysis using a combined dataset of internal transcribed spacer, β-tubulin, and RPB2 sequences revealed that these species form a distinct group separate from other known species. Further investigations included detailed macro- and micromorphological studies, as well as molecular analyses to establish their taxonomic positions more precisely.

Tricholoma matsutake is a commercially valuable fungus that produces gourmet mushrooms. The artificial cultivation method that is currently used to produce fruiting bodies involves the transplantation of pine trees infected with T. matsutake from their natural habitats and the successful infection of young pine trees and mushroom production have been reported in Hongcheon. In this study, T. matsutake genetic diversity and relationships in this region were investigated. A genotype analysis was conducted on 25 fruiting bodies collected from an area approximately 2.56 km2 in size using 10 simple sequence repeat (SSR) markers. The analysis revealed 25 genotypes and 23 alleles with mean observed (HO) and expected (HE) heterozygosities of 0.312 and 0.293, respectively. The average polymorphism information content (PIC), which indicates marker polymorphism, was 0.257. A phylogenetic analysis showed no clear correlation between collection location and genetic distance; a spatial autocorrelation analysis indicated no significant genetic structure within 1600 m; and the Mantel test showed no significant correlation between genetic and geographic distances. These results were consistent with previous reports on Japanese populations. This study provides insights into the genetic diversity and structure of T. matsutake at a local scale and highlights the need for further studies across broader geographical ranges.