본 연구는 낙지다리(Penthorum chinense Pursh) 추출물을 기능성 화장품소재로 개발하기 위해 (−)‑epicatechin gallate를 지표성분으로 선정하고, 품질관리를 위해 High Performance Liquid Chromatography (HPLC)를 이용하여 분석법을 개발하였다. 분석에 사용된 칼럼은 Unison US-C18 (4.6 × 250 mm, 5 μm, Imtakt, USA)을 사용하여 0.05% (v/v) trifluoroacetic acid (TFA)와 메탄올을 이동상 조건으로 컬럼 온도는 30 ℃ 에서 유속은 1.0 mL/min 로 검출파장은 280 nm에서 검출하였다. International Conference on Harmonization (ICH) 가이드라인(version 4, 2005)을 근거로 하여 특이성, 직선성, 정밀성, 정확성, 검출한계 및 정량한계를 분석하여 분석방법을 검증하였다. 검출한계 및 정량한계는 각각 0.11 mg/mL 및 0.33 mg/mL로 나타났으며, 검량곡선은 상관계수값이 0.9999로 양호한 직선성을 보였고, 정밀성 분석결과 도 0.6% 이하로 확인하였다. 또한, 회수율은 99.51 ~ 101.92% 범위로 정확성이 있음을 알 수 있다. 따라서, 본 분석법은 낙지다리 추출물의 지표성분의 분석법은 적합한 시험법임이 검증되었다.

Background : Aralia continentalis Kitag. (syn. = A. cordata Thunb., Araliaceae) is a traditional medicinal herb spread widely in northeastern Asia, including Korea, China, and Japan. Many constituents from its root extracts, including diterpene and essential oils, have been isolated as active components for antioxidant, anti-inflammatory, analgesic, sedative, antifungal, anti-thrombotic, and growth inhibition. In this study, we describe the structural determination of the two new compounds. Materials and Results : The roots of A. continentalis were collected in Namwon-si, Jeollabuk-do, Korea, in March 2018 and identified by one of the authors (Dr. S. S. Hong). The roots of A. continentalis were extracted with 70% EtOH two times at room temperature. The concentrated residue was subsequently suspended in H2O and partitioned with n-hexane, CH2Cl2, EtOAc and n-BuOH. The n-hexane, CH2Cl2, and EtOAc layer were subjected to sequential column chromatography over silica-gel, RP-18, MPLC and preparative HPLC to isolated the compounds 1 - 30. Consequently, a new nor-ent-pimaran diterpenoid (11) and a new 8-O-4′ type neolignan (25) along with 28 known compounds that included diterpenoids, phenolic derivates, and polyacetylenes have been isolated from the ethanol extract of the roots of A. continentalis. The structures of the new compounds were established by spectroscopic data interpretation, particularly HRESIMS, 1D and 2D NMR data including HSQC and HMBC. Also, those of the known compounds were identified by spectral comparison with those of the reported values. Conclusion : The structures of two new compounds were determined as 18-nor-ent-pimara-9 (11),15-diene-4β-ol (11) and 8-O-4-dehydrocoumaroyl-ferulic acid (25). To our knowledge, compounds 10, 14, 15, 19, 20, 22 – 24, and 26 were isolated and identified from Aralia genus for the first time.

Background : Despite the presence of various bioactive compounds in ginseng, there is lack of study on the variations of bioactive compounds in ginseng according to the cultivation of soil and the applied fertilizer types (or amount). Therefore, this study aims to examine the variations of 37 fatty acids (FA) and 8 vitamin E (Vit-E) vitamers in 6-year-old ginseng root cultivated in different soil types with different fertilizers regimes. Methods and Results : The profiling of 37 FAs and 8 Vit-E vitamers in 6-year-old ginseng roots was measured by gas chromatography coupled with a flame ionization detector, and then these results were statistically analyzed with chemometrics. The FA and Vit-E content in ginseng roots varied significantly with respect to soil cultivation conditions due to organic fertilizer types and amounts used. Unsaturated FA in ginseng is approximately 2.7 fold higher than the saturated FA. Linoleic, palmitic, and oleic acids were the most abundant FAs found in the ginseng roots. Also, the major Vit-E vitamer found in ginseng root is α -tocopherol. In particular, the application of rice straw compost or food waste fertilizer was increased to create nutritionally-desirable FAs and bioactive Vit-E in ginseng root. In addition, phytonutrient profiling coupled with chemometrics can be used to discriminate the cultivation conditions of ginseng. Conclusion : This preliminary study extends our understanding about the variations of FA and Vit-E in ginseng root depending on cultivation conditions. Hence, these results can be useful as basic information for reliable ginseng production containing high amounts of phytonutrients in a paddy-converted field.

Background : Phenolic compounds were isolated from the twig of Broussoneita Kazinoki. Their structures were established on the basis of extensive spectroscopic (MS, 1D , and 2D NMR) data analysis and by comparison with the spectroscopic data reported in the literature. Methods and Results : The twig of B. Kazinoki were extracted 60% aqueous ethanol for 3 days at room temperature. The extract was filtered and concentrated by vacuum evaporator. And then, extract was partitioned using hexane, methylene chloride (MC), ethyl acetate (EtOAc), n-butyl alcohol (BuOH) and H2O, successively. The extraction was separated by using prep-HPLC, and the structure was analyzed by Mass spectrometry (MS) and 1H-, 13C-, 1H-1H COSY, HSQC, HMBC NMR data. Conclusion : These compounds were identified as chlorogenic acid (1), ferulic acid (2), p-coumaric acid (3), taxifolin (4), marmesin (5), 5-methoxy marmesin (6), pinoresinol (7), syringaresinol (8), quercetin (9), broussonin A (10), broussonin B (11), broussoflavonol A (12), broussoflavonol B (13), kazinol A (14), and 5,7,3',4'-tetrahydroxy-3-methoxy-8,5'-diprenylflavone (15).