바이오산업의 발전으로 의약품, 식품 등의 생산 과정의 분리/정제 공정에 사용되어 왔던 기존의 컬럼 크로마토그 래피를 대체하여 더 높은 처리효율을 갖는 막 크로마토그래피가 부상하고 있다. 본 연구에서는 서로 다른 기공 크기의 두 가 지 상용 셀룰로오스 아세테이트(Cellulose acetate, CA) 분리막을 탈아세틸화 과정을 통해, 리간드의 개질이 용이한 다공성 재 생 셀룰로오스 지지체를(Regenerated cellulose, RC) 제조하였다. 음이온 교환능을 부여하고자 grafting을 수행하였으며, 구체 적으로는 UV 중합법을 통해 4차 암모늄을 포함하는 음이온 교환 리간드(MAPTAC)를 부착하여 음이온 교환용 흡착막을 제 조하였다. 단백질 흡착 용량은 정적 흡착 용량(Static binding capacity, SBC)시험을 통해 총 단백질 흡착 용량을 측정했고, 동 적 흡착 용량(Dynamic binding capacity, DBC)을 측정하여 상용막과 비교 평가하였다. 성능 평가 결과 단백질 흡착량은 넓은 표면적에 의해 리간드 밀도가 높은, 기공 크기가 작은 순서로 높게 측정되었고, 상용 CA분리막을 탈아세틸화하고 리간드를 부착시킨 분리막(RC 0.8 + MAPTAC 43.69 mg/ml, RC 3.0 + MAPTAC 36.33 mg/ml)이 상용 막 크로마토그래피 제품(28.38 mg/ml) 대비 높은 흡착 용량을 보였다.

Black carbon (BC), which is mainly contained in fine particulate matters, is one of the typical anthropogenic air pollutants that are generated from the incomplete combustion process and discharged into the atmosphere, and its various health effects particularly on children have been a growing concern. In this study, BC and particulate matters were closely analyzed in an elementary school adjacent to a high- traffic road in a large metropolitan city. The investigation showed that black carbon behaved similarly to ultrafine dust of 0.3 μm or less in the air, accounting for 20%-40% of it. The occurrence of high concentration outdoor pollution influences the BC content in indoor particulate matters. The average I/O value was 0.7 during the class-hours, and 0.8 without students. However, when students played in the classroom, the range of BC concentrations varied from 0.25 to 1.15, wider than 0.41-1.13 without students. Although this study was conducted with regard to just one elementary school, it can be considered to represent the typical air quality status of domestic schools, and it is believed to present valuable data which can be utilized to assist with preparing measures to enhance the air quality management of schools.

본 연구는 아세트아미노펜, 비타민 C, 무수 구연산으로 구성된 해열진통 및 염증 완화제, 피버닐산의 사료첨가가 구제역(FMD) 백신의 접종으로 야기 될 수 있는 돼지의 부작용에 대한 감소 효과를 확인하기 위해 실시되었다. 8-9주령의 돼지(LYD) 30마리를 공시, 대조군(n=10), 구제 역 백신 접종군(n=10; T1), 구제역 백신+피버닐 산 투여군 (n=10; 백신 접종 후 5일 간 0.2% 피버닐 산 경구 투여, T2), 3개 그룹으로 배치하여 시험을 진행하였다. 각 시험 구에서 총 14일 동안 체중, 혈액학치, 혈액화학치, FMD 항체 및 스트레스 지표의 변화를 관찰했으며, 총 증체량 은 T1(11.13±1.33kg)에 비해 T2(13.76±1.73kg)에서 유의적 으로 증가하였다(p<0.001). 혈액학치 및 혈액화학치 분석 결과, 백신을 접종한 두 처리군에서 유의한 차이는 없었 다. FMD 항체 역가는 T1(89.1±7.9)에 비해 T2(91.9±5.6)에 서 증가하는 경향을 보였으나 통계적 유의성을 나타나지 않았다. T2 그룹의 코르티솔, TNF-α 및 CRP와 같은 스 트레스, 염증 지표 물질은 구제역 백신 접종 후 3일에서 5 일 사이에 각각 310.31±44.11ng/mL, 59.05±11.88pg/mL, 29.05±2.84ng/mL으로 T1(361.58±58.74ng/mL, 74.47±19.26 pg/mL, 34.21±5.34ng/mL)보다 유의적으로 낮게 측정되 었다. 시험 14일째에 육안 및 조직병리학적 분석 결과, 주 사 부위의 염증 반응은 T2에서 적게 관찰되었으며 염증세 포 침윤 및 섬유조직이 증식된 육아종성 염증 또한 T2에 서 적게 관찰되었다. 본 연구결과로 미루어 보아 피버닐 산의 0.2% 경구 투여는 체중감소, 발열 등의 임상증상을 감소시키는 효과가 있으며, 접종 부위의 염증반응을 억제 하여 구제역 백신 접종의 부작용에 대한 스트레스를 경감 시키는 것으로 판단된다. 따라서 백신 접종 후 피버닐 산 의 0.2% 경구 투여는 돼지에서 구제역 백신 접종과 관련 된 부작용을 줄이는 데 효과적인 것으로 사료된다.

본 연구는 전화코칭을 병행한 운동프로그램이 지역사회 거주 독거노인의 균형능력, 악력 및 우 울에 미치는 효과를 조사하기 위한 단일집단 사전사후 원시실험연구이다. 연구대상자는 경기도 S시에 위치 한 노인복지관에 등록된 독거노인 20명이 참여하였으며, 자료수집기간은 2021년 7월 03일부터 2021년 10 월 14일까지 총 12회기 프로그램을 시행하였다. 프로그램 전후에 대상자 가정에 직접 방문하여 4단계 균형 검사, 근력검사, 악력 및 우울을 측정하였다. 중재 프로그램은 탄력밴드와 악력볼을 이용하여 구성하였으며 매주 전화코칭 중재를 제공하였다. 연구결과, 전화코칭을 병행한 운동 프로그램은 대상자의 4단계 균형기 능(3단계)(t=-2.37, p=.029), 4단계 균형기능(4단계)(t=-2.46, p=.024), 우측 악력(t=-2.18, p=.042), 우울 (t=2.82, p<.001)에서 통계적으로 유의한 차이가 나타났다. 본 연구 결과를 바탕으로 전화코칭을 병행한 운 동프로그램은 독거노인의 균형능력, 악력 향상 및 우울 감소를 위한 간호중재로 활용될 수 있을 것으로 기 대된다.

식물공장에서 적합한 광을 선정하기 위해서는 양적인 측면 과 기능적 측면 뿐만 아니라 운영비를 고려하기 위하여 전기 에너지이용효율과 광이용효율을 동시에 고려해야 하는데 본 연구에서는 들깨를 위한 LED광원의 광량, 적색광과 청색광 의 혼합 비율과 광주기 조건별 생육 특성과 전기에너지이용효 율과 광이용효율을 함께 비교하였다. 광량 처리구는 60, 130, 230, 320μmol·m-2·s-1 조건으로, 광질 처리구는 적색광과 청 색광의 혼합 비율 8:2, 6:4, 4:6, 2:8 조건으로, 일장 처리구는 낮 기준 9, 12, 15, 18시간으로 처리하였다. 광량 실험에서는 광량이 높을수록 생육량이 늘어나는데 비해 소비전력당 건물 중의 광이용효율은 유의차가 없었다. 소비전력당 엽생체중을 추가로 비교해보면 320μmol·m-2·s-1 처리구에서만 유의적으 로 낮은 효율을 보였고 이외의 처리구에서는 유의차가 없었기 때문에 생산량이 가장 많은 230μmol·m-2·s-1가 가장 효율적 이었다. 광질 실험에서는 적색광과 청색광의 혼합 비율은 RB 8:2에서 생육량과 광이용효율이 동시에 높게 측정되었고 색 차와 flavonoids 함량에서는 유의차가 발생하지 않아 Red: Blue 비율 8:2가 가장 적합한 조건이었다. 광주기 실험에서는 광주기가 길어질수록 높은 생육량을 나타냈는데 일장 12시간 이상에서는 생육량의 유의차가 없었으므로 광소비 효율을 고 려한 12시간이 적합한 조건이었다. 이상의 결과를 바탕으로 식물공장에서 들깨 생육을 위한 LED 광 환경 조건으로는 광 도, 광질과 일장은 각각 230μmol·m-2·s-1 이상, 8:2와 12시간 이상이었다.

Microcystins (MCs) are cyano-toxins mainly produced by cyanobacteria in the genera of Microcystis, Anabaena, and Oscillatoria. The concentrations of MCs in the water bodies and fish tissues taken from the four weirs (Ipo, Gangjeong-goryeong, Baekje, and Juksan) in the four main rivers in Korea, and the health risk of human due to consumption of toxin-detected fish was examined. The maximum values of MCs concentration in the water samples were as follows: Juksan (3.261 μg L-1), Gangjeong-goryeong (1.014 μg L-1), Baekje (0.759 μg L-1), and Ipo (0.266 μg L-1) weirs. The MC-RR concentration was the highest among the MCs, and MC-YR was not detected. MCs of 0.222~9.808 μg g-1 dry weight were detected in the liver of 3 out of 215 fishes of 16 species, and below the detection limit in muscle. As a result of comparing the feeding characteristics of the collected fishes and toxin concentrations in water and fish tissue, it was concluded that the biomagnification of MCs through the food chain did not occur. It was judged that there was no health risk due to the consumption of the fish detected the toxin, based on the amount of the fish intake of the Korean people and the allowable daily intake of MCs. However, in order to reduce the health risk due to MCs, further studies should be conducted to analyze the concentration of MCs contained in fish tissues collected at various times in the area dominated by harmful cyanobacteria to obtain data on the exposure of MCs due to fish consumption. In addition, it is necessary to establish the management guidelines for MCs in fish tissues.

The occurrence of cyanobacterial blooming and the contaminant sources were analyzed in the downstream of Jecheon Stream, a tributary of Chungju Reservoir. The concentrations of chlorophyll a at the Myungseo Fishing Point (GPS; 37°03′25.5″N, 128°03′13.6″E) were 399.2 and 184.8 mg m-3 on October 18, 2015 and September 25, 2016, respectively, and the concentrations of total microcystins, a cyanobacterial toxin mainly produced by Microcystis, were 124.09 and 79.71 μg L-1, respectively. The occurrence of cyanobacterial blooming at the downstream of Jecheon Stream was closely related to the water level of Chungju Reservoir. The cyanobacterial blooming occurred after the increase of water level in Chungju Reservoir, when the water body stagnated. As a result of analyzing National Water Quality Monitoring Data of the upper region of Jecheon Stream, the main source of pollutant was Jangpyeong Stream, the tributary of Jecheon Stream, and the discharge water from Jecheon Wastewater Treatment Plant located in Jangpyeong Stream was considered to be the most important source of contaminant.

This study aims to analyze mediating effect of social outcome between value realization efforts and economic outcome of social entrprises. Data for the analysis is collected from Social Enterprise Promotion Agency. The results of anaylsis are first social value realization efforts had no positive effect on economic outcome of the social enterprises. But social outcome had a significant mediating effect between social value realization efforts and economic outcome. Through this, it was confirmed that although a social enterprise is a hybrid organization that must pursue both social and economic outcome, social value realization efforts go through a social outcome that affects economic outcome. mediating social performance This is expected to have implications for the role of social value realization efforts and social outcome in order to increase the economic outcome of social enterprises.

A field study was conducted to reduce airborne bacteria by supplying active ions to indoor spaces used by vulnerable human groups spending substantial amounts of time in places such as schools and hospitals. In an experiment conducted during school hours (8:00-15:00), the average number of airborne bacteria in classrooms was 345.53 CFU/m3 or more without active ions. However, ion supply reduced the airborne bacteria to an average of 113.23 CFU/m3, indicating an efficiency of 61.61%. As a result of tests in 33 rooms used for surgery in small and middle sized hospitals, ion supply for 2 to 4 hours reduced the average airborne bacteria concentration from 243.88 CFU/m3 to 104.34 CFU/m3, representing a 41.53% reduction. A laboratory test to confirm the ion activity has shown that the mortality rate of E. coli used as a test bacterium increased with exposure time to ions. The initial colony number of E. coli was 251 CFU, but decreased to 4 CFU after 60 minutes of exposure to active ions. Therefore, it was confirmed that the supply of active ions can contribute to the control of airborne bacteria in the indoor environment of schools, hospitals and other public facilities.

The present study was conducted to examine the effect of soybean silage as a crude protein supplement for corn silage in the diet of Hanwoo steers. The first experiment was conducted to evaluate the effect of replacing corn silage with soybean silage at different levels on rumen fermentation characteristics in vitro. Commercially-purchased corn silage was replaced with 0, 4, 8, or 12% of soybean silage. Half gram of the substrate was added to 50 mL of buffer and rumen fluid from Hanwoo cows, and then incubated at 39°C for 0, 3, 6, 12, 24, and 48 h. At 24 h, the pH of the control (corn silage only) was lower (p<0.05) than that of soybeansupplemented silages, and the pH numerically increased along with increasing proportions of soybean silage. Other rumen parameters, including gas production, ammonia nitrogen, and total volatile fatty acids, were variable. However, they tended to increase with increasing proportions of soybean silage. In the second experiment, 60 Hanwoo steers were allocated to one of three dietary treatments, namely, CON (concentrate with Italian ryegrass), CS (concentrate with corn silage), CS4% (concentrate with corn silage and 4% of soybean silage). Animals were offered experimental diets for 110 days during the growing period and then finished with typified beef diets that were commercially available to evaluate the effect of soybean silage on animal performance and meat quality. With the soybean silage, the weight gain and feed efficiency of the animal were more significant than those of the other treatments during the growing period (p<0.05). However, the dietary treatments had little effect on meat quality except for meat color. In conclusion, corn silage mixed with soybean silage even at a lower level provided a greater ruminal environment and animal performances, particularly with increased carcass weight and feed efficiency during growing period.

We investigated the standard cultivation substrate for Sparassis latifolia “Neowul” bred in Jeollabuk-do Agricultural Research and Extension Services. Cultivation characteristics and yield were assessed by varying the kind of sawdust and additives, and the mixing ratio. The cultivation period in larch sawdust was the shortest at 97 days. The yield was excellent (143.6 g). The findings indicated that larch is a tree species appropriate for the cultivation of S. latifolia. When the additives were varied, the yield and productivity (53.1%) were highest (116.6 g) for the wheat bran treatment. Thus, wheat bran would be an additive appropriate for culturing S. latifolia. Concerning the use of different mixing ratios, larch sawdust:beet-pulp:wheat bran ratios of 80:15:5 and 85:10:5 resulted in yields of 114.4 g and 111.4 g, and productivity of 52.5% and 51.8%, respectively. These yield and productivity values were not statistically different. Thus, the standard cultivation substrate for S. latifolia can comprise larch sawdust, beet pulp, and wheat bran at a ratio of 80:15:5 or 85:10:5. The carbon/nitrogen (C/N) ratio assumed to be appropriate for the cultivation of S. latifolia was 184 –223. Pinheading would be difficult below a C/N substrate ratio of 105. Thus, the C/N ratio of the media, as well as the pH, would be vital factors affecting pinheading during S. latifolia cultivation.

This study evaluated whether fevernill, which is primarily composed of acetaminophen, vitamin C, and anhydrous citric acid, is effective in relieving stress caused by foot-and-mouth disease virus (FMDV) vaccination in calves. Three-month-old calves from a farm in Korea were randomly assigned to one of three groups (n = 10 per group: control [untreated], group A [FMDV vaccination], and group B [FMDV vaccination + addition of fevernill 0.2% to feed for five days after vaccination]). Body weights, antibody formation, clinical symptoms, serum parameters were measured in the 14 days following vaccination. Total weight gain and average daily gain during the experiment period were group A (12.00±1.15 kg, 0.86±0.08 kg/day) compared to group B (13.57±0.98 kg, 0.97±0.07 kg/day) was significantly improved (p<0.05). There was no significant difference in the formation of antibodies against the FMDV vaccine between group A and group B, though the antibody value of group B tended to be higher than that of group A. The pre-inflammatory cytokine TNF-α value was 74.47±19.26 pg/ml in group A and 59.05±11.88 pg/ml in group B on the 5th day of the experiment, which was significantly lower in group B than in group A (p<0.05). Also, cortisol concentrations were significantly lower in group B than in group A (p<0.05). In conclusion, the feeding of fevernil is judged to be helpful in mitigating the side effects caused by FMD vaccination, and thus it is thought to be able to prevent the decrease in productivity caused by vaccination.

CALMET (California Meteorological Model), which is a meteorological subroutine for an air quality dispersion mode (CALPUFF; California Puff Model), closely related with the land surface structure. In this study, the sensitivity of micro-meteorological parameters including wind speed and roughness height, Albedo, Bowen ratio, soil heat flux, and leaf area index were closely evaluated with change of land-use in relation to urban development. As a result, although no consistent dependence of roughness height on surface wind was found, it showed that high value of surface roughness could lead to the increase of friction velocity, influence the Monin-Obukhov length and the mixing height. At the same time, the increasing Albedo reduced friction velocity and mixing height. Thus, it was concluded for the CALMET modelling that it is necessary to first define the roughness height, Albedo, and Bowen ratio according to land-use.