This study was performed to investigate antioxidant and anti-inflammatory activities of perilla(Perilla frutescens L.) seed, flower and leaf according to extraction condition. Perilla seed extracts(PSE), perilla flower extracts(PFE), perilla leaf extracts(PLE) was extracted by stirring extraction (STE, 25°C), shaking extraction (SHE, 80°C), and sonication assisted extraction(SAE, , 25°C) with 94% ethanol, 60% ethanol and distilled water, followed by analysis of total polyphenol and flavonoid and testing radical scavenging activities. The highest total polyphenol content (5.47, 9.36, 38.58 mg gallic acid equivalent/g), total flavonoid content(5.77, 8.62, 46.44 mg catechin equivalent/g), ABTS(10.68, 19.46, 63.56 mg trolox equivalent/g) and DPPH(6.51, 7.69, 79.73 mg trolox equivalent/g) radical scavenging activity of PSE, PFE and PLE was observed in the HWE with 60% ethanol,. Among the three extraction method, SHE provided the best results for yield, polyphenol, flavonoid content of perilla seed, flower, leaf in comparison to STE or SAE. SHE with 60% ethanol of perilla seed, flower, leaf more effectively inhibited secretion of nitric oxide(NO) and pro-inflammatory cytokine in RAW 264.7 macrophage exposed to LPS compared to other extraction solvent and method. Therefore, these extracts obtained from perilla seed, flower, leaf could be used antioxidant and anti-inflammatory ingredients in the food industry.
The objective of this study was to investigate the distribution of functional compounds in perilla leaves of various genetic resources and their antioxidant activities. A comprehensive analysis of functional compounds was conducted for 90 genetic resources, focusing on total polyphenol content (TPC), total flavonoid content (TFC), individual phenolic content (IPC), and lutein. Their antioxidant activities were then analyzed based on their radical scavenging capacity using ABTS (2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) and DPPH (2,2-diphenyl-1-picrylhydrazyl). The TPC content exhibited a range of 13.19 to 35.85 mg gallic acid equivalent/g, whereas the TFC content varied from 11.74 to 46.51 mg catechin equivalent/g. Total IPC was detected in a range of 6,310.98 to 40,491.82 μg/g. Lutein was detected at levels between 70.97 and 597.97 μg/g. ABTS and DPPH radical scavenging activities of perilla leaves ranged from 30.39 to 58.58 mg trolox equivalent (TE)/g and from 7.74 to 46.56 mg TE/g, respectively. Furthermore, correlation analysis demonstrated that rosmarinic acid, a phenolic acid, exhibited a significantly positive correlation with antioxidant activity. These findings suggest that various genetic resource of perilla leaves could effectively mediate antioxidant capacity. Results of this study provide valuable information for use of perilla leaves in Korea as functional food materials.
살리실산은 식물의 생장 및 발달, 항산화 방어기작, 광합성 작용 그리고 생물적 및 비생물적 스트레스 조건에서 다양한 생리적 기능을 조절하는 물질로 알려져 있다. 본 연구에서는 고온・건조 스트레스 조건에서 살리실산 경엽처리가 고추의 생육, 광합성 특성 및 항산화효소 활성에 미치는 영향을 구명 하고자 하였다. 광합성 특성 측정결과 광합성 속도, 기공전도 도 및 증산 속도가 증가하였고, 3회차 처리에서 가장 높았다. 세포내 MDA와 H2O2 함량은 살리실산 3회차 처리에서 현저 하게 감소하는 경향을 보였다. APX, CAT, POD 및 SOD 활 성이 현저하게 증가하였으며, 무처리 대비 최대 247, 318, 55 및 54% 증가하였다. 고추의 생육 특성은 무처리구와 유의한 차이를 보이지 않았으나, 상품 수량은 15% 정도 증가하였다. 이러한 결과들을 종합해 볼 때, 살리실산의 경엽처리는 고추 의 광합성 특성과 항산화효소 활성을 증진시켜 고온・건조 스트 레스에 의한 피해 경감에 긍정적 효과를 유발함을 확인하였다
북한의 채소류 생산량은 2001∼2010년 연평균 377만 톤 이었으나, 2011∼2020년 동안 340만 톤으로 10% 이상 하락한 것으로 알려졌다(KOSIS 북한통계, FAOSTAT). 주요 채소류는 양배추, 토마토, 호박, 오이, 가지, 고추, 양파/파, 마늘, 수박 등이며 전체 재배면적에 대한 정확한 통계 데이터는 제공되지 않는다. 본 연구는 북한의 식량 부족 해결을 위한 식량작물과 대표적인 채소작물인 고 추의 작부체계 연계 가능성 평가를 실시하였다. 전작물인 식량작물 밀, 보리를 6 월 하순 경 수확하고 후작물로 7월 상순에 고추를 재배하였다. 재배시험은 국립식 량과학원 연천시험지(연천군 도신면 신서리 11)에서 수행하였으며, 이 지역의 기 상환경은 북한 남서부지역과 유사하여 이 지역의 약 50%에서 적용이 가능할 것으 로 판단하였다. 고추 6품종은 2021.6.7. 육묘트레이에 파종하여 30일간 육묘한 후 7.6. 연천시험 지에 정식하였다. 파종 후 첫 꽃은 36∼37일 후에 피었으며, 개화 소요 일수(파종 후 첫 꽃이 80% 개화)는‘PR진대건’이 53일,‘아시아점보’,‘신통방통’등 5품 종은 51일이 소요되었다. 홍고추 1차 수확은 개화 후 64일(9.28., GDD 889℃) 경과 되었을 때 가능하였는데 수확량은 많지 않았다. 2차 수확은 10.13.(GDD 1,041℃)에 하였으며, 수확 당시 전체 착과된 과 중에서 홍고추 비율은 품종별 12.3∼39.9%로 수확 못한 청고추의 비율이 훨씬 높았다. 보통 건고추용 홍고추 수확까지는 개화 후 GDD 1,000∼1,300(45∼50일) 정도 소요되는 것으로 알려져 있으며 농가에서는 3∼5회 정도 수확한다. 본 연구에서는 정식 후 홍고추 2차 수확기까지의 GDD는 1,430℃(7.5.∼10.15.)로 2회 정도 수확이 가능하였으나, 10.17. 최저기온이 –3.5℃로 떨어져서 더 이상 홍고추 수확이 불가능 하였다. 결론적으로 식량작물인 밀, 보리 후작으로 고추를 재배하여 홍고추를 생산하는 작부체계는 재배안전성 및 연계성 이 낮음을 확인하였다.
This study was carried out the estimation on consumption patterns and consciousness of domestic forage for improvement of the quality of domestic forage. Although the cultivated area in South Korea of forage has increased significantly compared to the past, the self-sufficiency rate of domestic forage has increased to around 80% since 2010. Also, livestock farmers prefer to use import forage than domestic due to convenience of use. In Korean beef farms, the ratio of import to domestic forage was higher in domestic forage (import forage 3 : domestic forage 7). In the method of securing domestic forage, purchase of forage (55.6%) was higher than self-cultivation of forage (44.4%). The ratio of use by bailing type was shown in the order of rice staw rice straw (50.5%), domestic hay (15%), imported hay (12.5%), and total mixed ratio (10.7%). The preference of forage was in the order of amount of foreign matter, moisture content, price, feed value in Korean native cattle farm. The result of satisfaction with domestic and import forage showed that the satisfaction of domestic forage price was higher than import forage, while the moisture content and foreign matter of forage were lower than import forage. In addition, in the results of the satisfaction and importance of domestic roughage compared to imported roughage, satisfaction with imported roughage was generally high in all items except for price. As a result, in order to improve the satisfaction of domestic forage in Korean native cattle farm, it is necessary to minimize foreign matter in forage and increase hay production for moisture content uniform in forage.
The Korean stow net is a fishing method that utilizes the changing direction of the net entrance with the tidal current. This study attempted to obtain basic data from the recent offshore stow net fisheries to improve the gear by analyzing the dynamic behavior of the nets affected by current speed and direction using computer simulations. A numerical calculation was performed at a current speed of 0.5 knot between 2.5 knot at each 0.5 knot. The time taken for the gear opening was the longest from 0.5 knot at 1,500 seconds and the shortest from 2.5 knot at 450 seconds in the simulations. In all cases, the net width and tension at net deployment gradually decreased as the current speed decreased. However, the net height tended to increase inversely proportional to the current speed. During the net rotation, the net height was maintained at all cases. The net width and tension fluctuated, but the regularity was very low. In this study, the calculated simulation data showed that the opening efficiency decreased proportional to the current speed. The opening efficiency is related to the catching efficiency; therefore, it is necessary to improve the gear to enhance its opening efficiency.
The research was analyzed the catch data of the five years (2016-2020) for six joint venture company tuna purse seiners in the Atlantic Ocean, with the aim of suggesting improvement measures for responsible and sustainable fishing according to changes of recommendation by International Commission for the Conservation of Atlantic Tuna (ICCAT) on the tropical tunas. In the last five years, the average catch of six tuna purse seiner gradually increased to 7,745 tons, 8,364 tons and 9,053 tons from 2016 to 2018, but decreased to 7,761 tons in 2019 and 6,214 tons in 2020. The reason for the decrease in fishing volume in 2019 and 2020 was the fluctuation of the formation of the cold water zone (22-23℃), and the total ICCAT convention area of FAD closure in January and February due to the expansion of the FAD closure area and poor free school catching during two months period, respectively. The analysis on fishing area showed that the percentage of fishing in the high sea was about 85% although the FAD closure area included the EEZ zone in coastal countries; the rise of the fishing license in coastal countries is also believed to be a factor. In order to overcome such situations and improve catching volume, it will be possible if excellent manpower is secured, school fishing is expanded, and the production of high value-added catch (purse seine special: PS).
The breakdown of the Korea-Japan fishing negotiations since 2016 has made it impossible for many domestic fishing vessels to operate in the Japanese EEZ water, and large purse seine fishing vessels are one of them. Mackerel is a highly migratory fish species, circulating in the East China Sea, South Sea, West Sea and East Coast throughout the year, and goes through a period of wintering, spawning, growth and feeding migration. In this study, in order to find out the dependence of large purse seine fishing vessels on mackerel fishing in the Japanese EEZ waters, we analyzed the characteristics of mackerel by large purse seine fisheries in the Japanese EEZ waters from 2010 to 2016. The catch and fishing details were investigated. The total catch of mackerel by large purse seine fishery, the average catch rate of mackerel in Japanese EEZ is 4 percent per year. The amount is estimated at 10.2 billion won per year. Although there was an effect, it was not judged to be a significant factor, and it was found that the amount of catch and fish price in the domestic waters had a greater influence on the total catch than the Japanese EEZ waters.
PURPOSES : In this study, the installation standards for micro-roundabouts at the intersection of local streets with two entering lanes of a 3.0 m lane width were developed.
METHODS : The inscribed circle diameter where a design standard vehicle could turn around safely based on the lane width and speed was analyzed using AutoTURN software. A total of 864 analysis scenarios for different entering volume and left-turning ratio (10%, 20%, 30%, and 40%), intersection type (three-way and four-way), heavy vehicle ratio (0%, 5%, and 10%), and circle lane turning speed (10, 15, and 20 km/h) conditions were established and evaluated. VISSIM Micro-Simulation was used to estimate the entering volume for microroundabouts with LOS C. RESULTS : The results showed that the estimated entering volumes of the three-way and four-way micro-roundabouts with LOS C were 300~400 and 200~400 vehicle/hour/lane, respectively. CONCLUSIONS : It is demonstrated that the standard volume of entering vehicles for micro-roundabouts is approximately 200 vehicle/ hour/lane, considering the number of approach lanes, designed speed of circulating lanes, left turn ratio, and heavy vehicle ratio.
Environmental fundamental facilities have different odor emission characteristics depending on the type of treatment facilities. To overcome the limitations of the olfactometry method, research needs to be conducted on how to calculate the dilution factor from the individual odor concentrations. The aim of this study was to determine the air dilution factor estimated from manually measured concentration data of individual odor substances (22 specified odor species) in three environmental treatment facilities. In order to calculate the optimum algorism for each environmental fundamental facility, three types of facilities were selected, the concentration of odor substances in the exhaust gas was measured, and the contribution of the overall dilution factor was evaluated. To estimate the dilution factor, four to six algorism were induced and evaluated by correlation analysis between substance concentration and complex odor data. Dilution factors from O municipal water treatment (MWT) and Y livestock wastewater treatment (LWT) facilities showed high level of dilution factors, because concentration levels of hydrogen sulfide and methylmercaptan, which had low odor threshold concentrations, were high. In S food waste treatment (FWT) facility, the aldehyde group strongly influenced dilution the factor (dominant substance: acetaldehyde, i-valeraldhyde and methylmercaptan). In the evaluation of four to six algorism to estimate the dilution factor, the vector algorism (described in the text) was optimum for O MWT and Y LWT, while the algorism using the sum of the top-three dominant substances showed the best outcome for S FWT. In further studies, estimation of the dilution factor from simultaneously monitored data by odor sensors will be developed and integrated with the results in this study.
Trawl fishing is a fishing method in which a large, motorized trawler tows a bag-shaped net to catch fish living at the bottom or middle layers. For a trawl gear, it is of utmost importance to select the gear size and towing speed suitable for the effective horsepower (EHP) of the trawler in the design stage. In general, the power required to move an object is proportional to the product of the object speed and resistance; therefore, there are various choices for the gear resistance and towing speed given the effective horsepower of the trawler. However, there have been few studies on the gear design of an appropriate scale for the towing speed given the effective horsepower of the trawler. In this study, the resistance and shape of three types of midwater trawl gears were analyzed using SimuTrawl, a computer simulation tool. In addition, the relationship between the propulsion force and speed of the ship was clarified when the size and effective horsepower of the trawler were determined. Finally, we suggested the relationship between the towing speed and the resistance of the gear when the trawler towed the net was investigated, and a specific method of selecting the gear size according to the towing speed.
본 연구에서는 2008년 1월부터 2019년 10월까지 보고된 국내외 출간・비출간 연구를 대상으로 오락용 게임이 일반 사용자의 공격성, 사회성 및 정서에 미치는 영향에 대한 다변량 메타분석을 시행했다. 총 22개 관련 연구(효과 크기 수 = 54개, 참여자 수 = 8031명)에 대한 메타분석 결과에 따르면, 게임의 폭력성 또는 폭력적 게임의 이용이 사용자의 정서기능에 미치는 영향은 크지 않았다. 진실험연구 분석 결과에서만 게임의 폭력성 또는 폭력적 게임의 이용이 사용자 의 공격적 인지와 친사회적 행동에 약한 수준의 부정적 영향을 미치는 것으로 나타났다. 뿐만 아니라 공격적 행동과 부정적 감정(예를 들면 불안, 우울 등)에 미치는 영향은 진실험, 준실험 및 상관회귀 연구 모두 통계적으로 유의미하지 않았다. 조절변수에 대한 메타분석 결과, 게임의 폭력성 또는 폭력적 게임 이용의 효과는 아동보다 청장년에게서, 남녀의 비율이 균등하게 통제된 연구보다 남성의 비율이 더 높은 연구에서, 그리고 방법론적 질이 높은 연구보다 방법론적 질이 낮은 연구에서 더 크게 나타났다. 본 연구의 결과는 게임의 폭력성 또는 폭력적 게임 이용이 사용자의 공격성에 미치는 영향은 크지 않으며, 이 또한 사용자의 연령과 성별 그리고 연구의 방법론적 질에 따라 좌우될 수 있음을 시사한다.
In this study, the risk factors of coastal purse seine fisherman were analyzed through a survey of fishery workers of coastal purse seine fishery and the accident compensation insurance data of the fisheries workers of the National Federation of Fisheries Cooperatives (NFFC). The classified fishing operation accident data was analyzed through 4M (Man, Machine, Media, Management) model of the National Transportation Safety Board (NTSB) and the accident prevention measures were presented using Harvey's 3E (Engineering, Education, Enforcement) model. The rate of accidents on coastal purse seinens each year was 75.8‰, 36.7‰ and 74.8‰ from 2015 to 2017. The accident frequency resulting from slipping was the highest, and the risk of a contact with gear was low. When comparing each insurance data, the average value of the contact with gear accident was the highest. This research result is expected to be important data in identifying and preventing safety hazards of coastal purse seiner fisherman in the future.
The objectives of this study were (i) to evaluate the effects of temperature and relative humidity on two electrochemical sensors measuring hydrogen sulfide and ammonia using a laboratory testing system for various sensors, and (ii) to propose a calibration method for those concentrations to collect more reliable monitoring data. The effect of temperature and relative humidity was tested under three different conditions, respectively. The linearities measured data under all different conditions for the relative humidity and temperature were excellent, indicating more than 0.99 of R2 for both odor sensors. Under the condition of zero concentration, baselines (intercepts) at zero increased with increasing relative humidity for both hydrogen sulfide and ammonia sensors. The rate of gas concentration according to ADC variation (slopes) increased with increasing relative humidity about only the hydrogen sulfide sensor. In this study, slope, and intercept are utilized for calibration of hydrogen sulfide and ammonia concentration, and the reliability of the data of hydrogen sulfide and ammonia sensors is further enhanced by the relational expression obtained by this paper.