본 연구에서는 가물치(Channa argus) 추출물의 신경세포 분화와 산화 스트레스에서의 효능을 분석하기 위하여 녹차와 효소를 이용한 다양한 추출 방법(상온 추출물, RE; 녹차 상온 추출물, GRE; 효소 상온 추출물, ERE; 녹차 효소 상온 추출물, GERE)을 사용하여 제조 된 추출물의 아미노산 조성과 항산화 활성을 비교 분석하였고, 신경성장인자 (NGF) 유도 신경세포 분화 및 과산화수소 처리에 의해 유도된 PC12 세포 독성에 대한 보호효과를 규명하고자 하였다. 총 아미노산 함량은 RE 및 GRE보다 효소 추출물인 ERE 및 GERE에서 훨씬 더 높았다. 효소 가수 분해물 (ERE 및 GERE)에서 ABTS 라디칼 소거 활성은 RE 및 GRE보다 높았다. 또한, RE와 ERE는 PC12 세포에서 neuronal growth factor (NGF) 매개 신경 돌기 성장뿐만 아 니라 growth associated protein (GAP)-43 및 synapsin-1의 발현을 현저하게 향상 시켰다. 과산화수소(H2O2)에 의해 손 상된 PC12 세포에 4가지 유형의 Channa argus 추출물을 첨가한 후 PC12 세포의 생존율을 측정하였다. PC12 세포 의 생존율은 RE, GRE, GERE에서 각각 77.5±1.9%, 84.0±0.8%, 81.1±0.9%이였다. 이러한 세포 생존율은 H2O2 만을 처리 한 음성 대조군(70.0±2.0%)에 비해 더 높았다. H2O2 처리에 의해 유도 된 세포 독성도 RE, GRE 및 GERE 처리에 대한 반응으로 상당히 완화되었다. 종합하면, Channa argus 추출물은 산화 스트레스와 신경 손상을 감소시키는 기능성 물질로 유용하다는 것을 시사하며, 향후 이들 소재를 활용한 다양한 기능성 제품의 개발이 필요할 것으로 판단된다.

The study investigates the effects of corpus-based formulaic sequences learning on developing learners’ writing skills and attitudes. For this purpose, fifty-four high school students participated in the study and were divided into two groups. The experimental group learned formulaic sequences with the corpus-based method, while the control group learned the target items through the definition-centered method. The results of the study showed that no significant difference in writing ability was found between corpus-based formulaic sequences learning and traditional formulaic sequences learning. The corpus-based formulaic sequences learning showed a greater effect on improving grammatical accuracy of writing. The traditional formulaic sequences learning was effective in the acquisition of productive knowledge of formulaic sequences. The results of the survey questionnaire showed that the students showed a positive attitude toward corpus-based formulaic sequences learning, which may mean corpus-based learning can play an important role in increasing students’ motivation. These results may suggest that various corpus-based activities for EFL class need to be developed.

The purpose of this study is to find out the determinants of export in Korean fishery products. For the analysis, laver and tuna, which account for almost half of seafood exports, were selected, and a gravity model widely used in trade analysis was applied. As explanatory variables, GDP, number of overseas Koreans, exchange rate, FTA, and WTO were applied, and fixed effect terms were included to take into account multilateral resistance that hinders trade. The analysis period is from 2000 to 2018, and the Poisson Pseudo Maximum Likelihood (PPML) method was applied to solve the problem of zero observation and heteroscedasticity inherent in trade data. As a result of the analysis, GDP was found to have a significant positive effect on both laver and tuna. The number of overseas Koreans was significant in canned tuna exports, but not in laver and the other tuna products. As the exchange rate increased, the export of laver and tuna for sashimi increased. The impacts of the FTA were confirmed in the exports of dried laver and raw tuna, which supports the results of the previous study. WTO was not significant for laver and tuna. Based on these results, it is necessary to find a way to make good use of the FTA to expand exports of seafood.

The aim of this study was to investigate the effects of timing of collecting date and concentration of IBA and NAA, in order to enhance initial activity and seedling quality of domestic strawberry. Strawberry cuttings were separately taken twice, in June 7 and in July 5, and IBA and NAA were treated with the concentrations of 0.025, 0.05 and 0.1% at cutting date, respectively. The seedlings were evaluated for the percentage of survival during 18 days at 6 times after tunnel cultivation. The NAA treatment was inappropriate for strawberry cutting due to the high rate of seedling mortality, regardless of the collecting date. The vitality of the seedlings was highest at IBA 0.1% in June collecting and at IBA 0.05% in July collecting. The seedlings from June collecting had a higher quantum yield at IBA 0.1% and the seedlings from July collecting at IBA 0.05%. Therefore, IBA could be more effectively applied than NAA to promote the vitality and quality with the appropriate concentration of 0.1% at June collecting and 0.05% at July collecting, respectively.

This study was conducted to find out the effect that κ-Carrageenan has on the properties of dog sperm when it was added to the cryoprotectant. Extender basically was contained 1.21 g Trizma base, 0.67 g citric acid, 0.4 g glucose, 0.03 g penicillin G, 0.05 g streptomycin sulfate. Extender1 was added with 0.1%, 0.2%, 0.3%, and 0.5% carrageenan, while extender2 was supplemented with glycerol. After freezing-thawing, the motility, viability, acrosome integrity, apoptosis, and ROS (reactive oxygen specifications) of sperm were measured to analyze the effects of the supplementation of carrageenan. Total Motile (TM), Rapid Progressive Motile (RPM), Medium Progressive Motile (MPM), and Immotile were measured through the CASA system after thawing in 37 degree water. Extender with 0.2% κ-carrageenan (64.26 ± 0.49) was significantly higher than control (40.24 ± 8.27) (p < 0.05). RPMs of extender with 0.1%, 0.2% κ-carrageenan (57.64 ± 6.34, 56.47 ± 1.35) were significantly higher than the other groups (p < 0.05). Acrosome integrity was measured by dyeing to PSA-FITC with an epifluorescence microscope. Normal acrosome ratio of extender with 0.5% κ-carrageenan (61 ± 8.03) was higher than the other groups (p < 0.05). Apoptosis was measured with a FACSCalibur flow cytometer using FITC (FITC Annexin V Apoptosis Detection Kit). Treated groups of κ-carrageenan of 0.1% (0.81 ± 0.05), 0.2% (0.85 ± 0.05) were significantly higer (p < 0.05) than control. Modified SYBR/PI staining was used for determination of viability and DCF staining was used for evaluation of ROS. Viability and ROS were not significantly different from other groups. In conclusion, adding a certain concentration of carrageenan to the extender of cryopreservation, carrageenan contributes to the improvement of the sperm motility, acrosome integrity and prevention of apoptosis.

얌빈(Pachyrhizus erosus, Yam bean)은 멕시코 원산의 아열대성 콩과 식물로서 아삭하고 즙이 많아 생으로 먹거나 다양한 요리재료로 사용되는 구근채소이다. 본 시험은 국내에서 얌빈 재배시 적정 파종시기를 찾고자 수행되었으며, 이를 위해 국내에서 주로 재배되는 2종을 대상으로 하여 온도에 따른 발아특성과 파종시기에 따른 적산온도 및 생산성을 비교하였다. 발아율은 18℃이상에서 86.0~94.0%를 보였으며, 발아 소요일수는 낮은 온도에서 길게 나타났다. 천군만마 품종이 태국 재래종에 비해 개화기나 구근 형성시기가 더 늦었고, 개화와 구근형성 및 비대에 필요한 적산온도도 각각 293℃, 280℃, 108℃가 더 소요되었으며, 지상부나 구근의 건물량은 더욱 많았다. 또한, 4월 25일 파종은 다른 시기에 비해 구근형성기까지 일수가 더 소요되었고, 구근형성과 비대시기의 지연으로 수확지수가 낮았으며, 6월 9일 이후 파종시는 영양생장이 충분하지 않은 상태에서 10월 하순 이후 저온 및 서리로 인해 생육이 정지되어 구근의 충분한 비대가 어려웠다. 한편, 5월 10일과 25일에 파종한 경우에는 일장, 온도 등의 변화가 영양생장과 구근형성에 유리하게 작용하여 얌빈 두 종 모두 구근 건물량과 수확지수가 높고 수량이 많아 파종시기로 적합하였다.

21세기 동시대 예술의 흐름은 글로벌 아트라는 경향 속에 있다. 글로벌 아트는 주된 예술의 중 심이 없는 탈 중심의 예술경향이다. 이는 곧 탈서구화의 의미이기도 하다. 1998년에 창립된 베를 린 비엔날레는 기존의 서구중심의 예술관점을 탈피한 운영정책 즉 탈서구화 정책을 펴고 있다. 젊 은 예술가들을 주축으로 동시대 예술을 고찰 하고자 하는 베를린 비엔날레는 현 시대 예술경향인 글로벌 아트의 이해와 정립을 시도하는 운영전략을 펴고 있다. 이 고찰을 통해, 본 연구는 세계화 속의 국제 비엔날레가 궁극적으로 개최 지역의 문화역사를 동반한 과정일 수밖에 없으며 이는 동 시에 지역예술 발전의 동반을 마련한다는 것을 설명한다. 이는 세계화로 긴밀히 밀착된 글로벌 아 트의 특성과 지역 예술 간의 밀접한 관계에 대해 재조명하게 할 것이다.

This study investigated the effect of high concentration of free ammonia on microalgal growth and substrate removal by applying real wastewater nitrogen ratio. To test of this, the conditions of free ammonia 1, 3, 6, 9, 12, 15 mg-N/L are compared. After 3 days of incubation, algal growth of Chlorella vulgaris and carbon removal rate are respectively lower in the reactors of FA 12, 15 mg-N/L compared to the others. This indicates that the high concentration of free ammonia, in this case, above 12 mg-N/L, has negative effect on algal growth and metabolic activity. Also, high concentration of free ammonia causes the proton imbalance, ammonium accumulation in algae and has toxicity for these reasons. So, we have to consider free ammonia in applying the microalgae to wastewater treatment system by the way of diluting wastewater or controlling pH and temperature.