한지형 마늘의 엽 제거 정도에 따른 피해양상을 구명코자 단양종 마늘을 시험재료로 출현 후 1엽, 2엽, 3엽, 4엽 제거 후 생육을 조사하였다. 엽생육은 4엽 제거에서 저조하였으며, 엽초경 또한 같은 경향을 보였다. 엽 면적은 무제거 및 1엽 제거에서 441.3 cm2/주 및 458.5 cm2/주 였으나 3엽 및 4엽 제거에서는 320 cm2/주로 엽 면적이 줄어드는 경향을 보였다. 조기출현정도별 생체중, 뿌리수를 조사한 결과 엽 피해가 클 수록 적어지는 경향을 보였고, 출현율은 일정한 경향을 보이 지 않았다. 초장, 엽수, 엽초장, 엽초경 등의 지상부 생육은 엽 제거 정도가 클수록 생육이 저조하였으며 화경생육 및 엽록소 함량은 일정한 경향을 보이지 않았다. 구중은 엽 제거 정도가 클수록 적어지는 경향을 보였고, 구형지수는 엽 피해가 클수 록 타원형의 형태를 보였다. 인편수 및 이차생장은 엽 피해와 관계없이 일정한 경향을 보이지 않았다. 엽 부위가 구 비대에 미치는 영향을 검토한 결과 제거시기에 관계없이 4번엽 제거 에서 구비대가 가장 억제 되었다. 엽 피해 정도별 수량조사결 과 무제거(946 kg/10a) 대비 4엽 제거(698 kg/10a)에서 26.2% 정도 감수되었다.

코끼리마늘의 적정 파종시기를 구명하기 위하여 단양과 군위지역에 파종시기를 9월 30일, 10월 10일, 10월 20일 및 10월 30일로 달리 하여 파종한 후 생육특성 및 수량을 조사한 결과는 다음과 같다. 1. 코끼리마늘의 출현율은 단양지역의 72.5%에 비해 군위지역이 8.4%p 높았으며, 출현기는 파종시기가 늦어질수록 출현소요일수는 짧아졌으며 추대기와 개화기는 단양지역에 비해 군위지역에서 각각 15일, 10일이 빨랐던 반면 파종시기 간에서는 차이가 없었다. 2. 지하부의 생육은 군위지역이 단양에 비해 구중, 구고, 구경, 자구 등 양호한 생육을 보였으며, 파종시기에서는 단양에서는 10월 10일, 군위지역에서는 10월 20일과 10월 30일에서 구중과 구고가 무겁거나 큰 경향을 보였다. 3. 코끼리마늘 수량은 단양에 비해 군위지역이 파종시기 간 차이가 있으나 1.5~ 2.3배 높았으며, 단양지역은 10월 10일, 군위지역에서는 10월 20일 이후에 많았다.

Background: For stable induction of tetraploidy in Fallopia multiflora Haraldson, colchicine was treated to establish the condition of induction and investigated the morphological and cytogenetic traits of the tetraploid plants obtained compared to those of diploid ones. Methods and Results: For the induction of tetraploidy, F. multiflora plants were soaked in aqueous solutions of colchicine at various concentration (0.1, 0.5, and 1.0%). After this, 2% dimethyl sulfoxide (DMSO) was added at room temperature on a shaker set at 150 rpm for periods of 12, 24, and 48 h. The induction rate of tetraploids appeared to be the highest in plants treated with 0.5% colchicine for 24 h. As the colchicine concentration and soaking time increased above these levels, the growing tip of the roots did not develop and they began to rot. When compared to diploid plants, tetraploids differed greatly in various characteristics, including the sizes and shapes of the leaves, fruits, flowers and roots. The induced tetraploid F. multiflora had larger guard cells, and chloroplasts, increased number of chloroplast in the guard cells and decreased stomatal densities. Conclusions: When colchicine induced plants for tetraploid, it can be distinguished from diploids, in various characteristics such as morphological changes as stomatal size, number of chloroplasts per guard cell, number of chromosomes and flow cytometry. Therefore, it proved that these methods are suitable, quick and easy methods for the identification of the ploidy level of F. multiflora.

Background : There are studies on the planting distance, sowing periods, and over wintering method of Saururus chinesis, but lesson the necessary soil moisture content for rhizome growth. This study examines the effects of soil moisture content on the growth of Saururus chinesis. Method and Results : The radicles of Saururus chinesis were harvested in the beginning of March and cut into three joints. The planting densities were determined as 40 ㎝ × 10 ㎝, 40 ㎝ × 20 ㎝ and 40 ㎝ × 30 ㎝, and the soil moisture content was adjusted through continuous flooding treatment, wetting (0 ~ -10 kPa), and field moisture capacity (-20 ~ -50 kPa). As a result of investigating the ground growth of Saururus chinesis, the growth through continuous flooding was best with the planting distance of 40 ㎝ × 20 ㎝ that yielded a plant height of 7.4 ㎝, stem diameter of 5.2 ㎜, and leaf number of 5.5. The growth through field capacity (-20 ~ -50 kPa) was poorest at the planting distance of 40 ㎝ ×10 ㎝. The transpiration rate, indicating the breathing of leaves, was highest at 1.4 s ㎝-1 through continuous flooding at 40 ㎝ × 30 ㎝, and decreased to 0.5 s ㎝-1 with the field capacity of (-20 ~ -50 kPa) 40 ㎝ × 20 ㎝. The investigation on the leaves showed that the leaf weight was heaviest at 23.1 g/plant through continuous flooding of 40 ㎝ × 30 ㎝ that also showed a wider leaf area and rich chlorophyll. At continuous flooding of 40 × 10 ㎝, the leaf weight was as light as 9.5 g/plant, showing no consistency among treatments. The investigation on the underground growth showed the best results through continuous flooding with a planting distance of 40 × 10 ㎝ where the root length was 50.7 ㎝, root diameter 6.7 ㎜, and fresh root weight of 45.3 g/plant, which decreased to 24.4 g/plant with field capacity (-20 ~ -50 kPa). The investigation on the polyphenol content as a functional component showed the richest content in the leaf of the plant at 752.5 ㎎/100g through continuous flooding, and lowest at 661.0 ㎎/100g with field capacity. With the field capacity (-20 ~ -50 kPa) it was the highest at 262.0 ㎎/100g. Conclusion : It was the most advantageous for mass Saururus chinensis radical production with continuous flooding treatment for soil moisture and a planting distance of 40 × 10 ㎝.

This study was performed to develop the mass propagation system using tissue culture technique to supply the seeds of Elephant garlic (Allium ampeloprasum L.) which has difficulty in propagation. Immature spathe of Elephant garlic was cultured on Murashige & Skoog (MS) medium supplemented with two plant growth regulators, naphthaleneacetic acid (NAA) and kinetin. After 6 weeks of culture, the highest number of shoot (14.9/explant) was obtained when the immature spathe with 10 ㎝ length was cultured right after harvesting. In MS medium supplemented with 2 ㎎/L kinetin and 0.5 ㎎/L NAA, the most vigorous growth characteristics was observed, the shoot number was 14.9/explant, its length was 11.3 ㎝, and its fresh weight was 2.5 g. When the bulblets were cultured in MS medium with 2 ㎎/L kinetin and 0.5 ㎎/L NAA, the addition of 30 ㎎/L adenine improved their proliferation and growth significantly, the highest bulblet formation rate (48%) was obtained. The addition of 7% sucrose also increased the bulblet formation rate at the highest frequency of 98.2%. The shoots were shown be more vigorously proliferated at the secondary subculture stage rather than primary culture stage, their propagation rate was 80% after subculture.

Background : Polygonatum is a perennial herbaceous crop that is further classified into P. falcatum A. Gray (wild vegetation of Jeju, Japan), P. sibiricum F. Delaroche (wild vegetation of Korea north, China, Russia, and Mongol), and P. stenophyllum MAx (wild vegetation of South of Danyang), with the herbal name of Rhizoma polygonati. Polygonatum is harvested after 5 - 6 years since planting, and the quality of the radicle is a major factor determining the quantity of harvest. This study presents the morphological characteristics to predict the later quantity of radicles when planting polygonatum, and develops the methods for the prediction. Methods and Results : This study investigated the ground-level growth and the quantity of radicles in underground in 5-years-old, 4-leaf, 5-leaf, and 6- or more leaved polygonatum planted in the Chungbuk Agricultural Research and Extension Services. According to the investigation on the ground-level growth by the number of leaves, the 5-leaved plant showed the best plant length and nodal leaf number; 171 ㎝ and 24/stock, respectively. On the other hand, the leaf number and leaf weight were as heavy as 85 leaves and 58.3 g/stock, respectively, in 6-leaved plants or plants with more leaves. The leaf area was as wide as 15.6 ㎠ with 5-leaved plant, and the chlorophyll was also high. The investigation on the underground growth showed the best results in 6-leaved plants or plants with more leaves: the root length was 23.8 ㎝, number of radicle node was 5.7, and root weight was 291 g. The quantity was highest at 2,148 ㎏/10 a with 6-leaved plants or plants with more leaves, and at the lowest at 907 ㎏/10 a in 4-leaved plants. The profit analysis showed a profit of 3,859,000 won/5 years in 6-leaved plants or plants with more leaves, which 86% higher than the 4-leaved plants. Conclusion : If plants with6-leaf or more leaves at the ground level are selected and used as radicles for new polygonatum packaging, the quantity of radicles has increased by 59% and the profit increased by 86%, compared to 4-leaved plants.

Background : This study was performed to investigate the morphological and growth characteristics and metabolomics approach for the diploid and tetraploid Polygonum multiflorum Thunb (PMT). Methods and Results : The present study was performed to investigate the effective induction method of tetraploid plants using colchicine treatment. To know the morphological and growth characteristics of PMT overground growing was investigated about the stem diameter, leaf length and width, a scape, inflorescence length, chlorophyll, seed length and seed width. Metabolite profiles from P. multiflorum were analyzed by an ultra performance liquid chromatography–quadrupole - time of flight (UPLC-Q-TOF) mass spectrometry (MS). The number of stomata and chlorophyll content was also higher in tetraploid. However, the physiological and morphological characteristics of the newly developed tetraploid showed noticeable results compared to the diploid plant. Also, the inherent characteristics of a newly acquired tetraploid also exhibited better results compared to diploid. Twenty one compounds including catechins from leaf and thirty compounds including stilbenes from roots were identified as major metabolites of PMT. The levels of most root metabolites except dethiobiotin, epigallocatechin gallate, procyanidin B1, epiafzelechin 3-O-gallate, and moupinamide from PMT were higher than those of diploid. The levels of 2,3-digalloylglucose, fallacinol, tricin, physcion-8-O-β-D-glucoside, torachrysone-8-O-β-D-glucopyranoside, questin, aloe-emodin 8-O-(6′-O-acetyl)-glucoside, polydatin, 2,5-dimethyl-7-hydroxychromone, 3,5,4'-trihydroxystilbene, aloe-emodin, myrciacitrin II and ω-hydroxyemodin from teraploid PMT were 342, 32, 31, 21, 11, 11, 9, 8, 6, 5, 4, and 2 times, respectively, higher. Conclusion : From above results, overground growing of tetraploid P. multiflorum is fairly better than that of native P. multiflorum.

Background : This experiment was conducted to select suitable industrial crop after Panax ginseng cultivation under the greenhouse in the middle area. Methods and Results : This experiment was conducted in greenhouse of Chungbuk Agricultural Research Service and extension services from 2015 and 2016 years. Ginseng grown four years in greenhouse. After cropping of Panax ginseng was transplanted Cynanchum wilfordii, Platycodon grandiflorm, Dioscorea opposita, and Codonopsis lanceolata. Non-cultivated field that were grown sesame. Cynanchum wilfordii, Platycodon grandiflorm, and Codonopsis lanceolata was collected in the Chungbuk area and Dioscorea opposita was collected in Andong-si. Each industrial crop after panax ginseng cultivation under the greenhouse was sowing from late April to early May 2015 years. The growth and yield of crop examined in the experiment were good on the whole except Codonopsis lanceolata without disease due to continuous cropping of Panax ginseng. The yield of Cynanchum wilfordii, Platycodon grandiflorm, Dioscorea opposita, and Codonopsis lanceolata was 803kg, 1,393kg, 1031kg, 412kg per 10a respectively, and the every decline yield index was by 1%, 8%, 12%, and 31% respectively. The proper crop in after cropping of Panax ginseng was cynanchum wilfordii, which was yielded 1,393kg per 10a by fresh tuber and it was 1% decline compared to the non-cultivated field. Conclusion : In this study, Cynanchum wilfordii, Platycodon grandiflorm, Dioscorea opposita was recommended as a crop after Panax ginseng cultivation under the greenhouse in the middle area.

Background:The study aimed to obtain data on the effects of cultivation and soil reduction of green manure crop on the quantity and quality of organically cultivated Cynanchum wilfordii Hemsley.Methods and Results:The experiment comprised four treatments: control, hairy vetch, barley, and hairy vetch + barley (3 : 2). The plant height in the hairy vetch treatment (86.3㎝) was significantly different from that in the other treatments, whereas the stem diameter leaf area, and special product analysis division (SPAD) value did not differ across the treatments. The largest soil reduction of green manure crop was recorded in the barley treatment (440 ㎏/10 a), whereas the smallest was recorded in the single treatment with hairy vetch (80 ㎏/10 a). The hairy vetch + barley (60 : 40) treatment showed 63% more soil microorganisms than control. Radical scavenging activity estimation revealed that the total polyphenol content was highest (1,740 ㎎/㎏), and the 2,2-diphenyl-1-picrylhydrazyl (DPPH) was 92.6% in the barley treatment. The 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) activation was highest in the control (51.1%), and the root yield was the highest in the barley treatment (310 ㎏/10 a).Conclusions:The root yield, total polyphenol content, and antioxidant activity of Cynanchum wilfordii (Maxim.) Hemsley increased in presence of the green manure crop barley.

Background : This study was investigated the effect of organic fertilizer application and stem training methods on the growth and yield of Cynanchum willfordii. Methods and Results : Traditional species, C. willfordii conducted the test to the field in Chungbuk ARES, Korea. Two methods, non-supporting (custom) and I-shape supporting was used for stem training method. Planting spacing was adjusted to 30 (interrow spacing) x 20 cm (intrarow spacing) and other key management is the followed the cultivation manual for standard medicinal crops. Organic fertilizer application is conducted in mid-March before planting of C. willfordii. Registered organic agricultural materials such as fungal cultures (CC), mixed organic materials (MO), fermented and mixed expeller cake (EC), and fermented fowl dropping (FD) was used. Application rate is based on the nitrogen application rate after soil testing. Plant height was both good in all at I-shape supporting (266.7 ㎝) and all in non-supporting (160.8 ㎝); however, stem diameter was more thicker in custom (4.6 ㎜) of I-shape supporting and EC (4.8 mm) of non-supporting. Number of branched stem were good at EC from I-shape supporting and non-supporting, as well as leaf growth. SPAD value was evaluated in MO (52.7) of I-shape supporting and EC (52.3) of non-supporting. Number of branched root per organic fertilizer were showed 7.0 at MO from I-shape supporting, but 7.3 at FD from non-supporting. While length of main root were 27.0 cm in MO of I-shape supporting and 31.3 ㎝ in FD of non-supporting. Root weight also more heavier in EC (66.3 g) of I-shape supporting and FD (53.0 g) of non-supporting. When applied organic fertilizer, total density of soil microorganisms were changed into 38.4 ppm in custom; however, it more plentiful of 90.7 ppm in MO, it showed good effect on the replication of soil microbiota. Conclusion : From the investigated results, MO of I-shape supporting was good at overall growth, including good tendency of roots growth.

Background : This study was performed to investigate the morphological and growth characteristics of tetraploid Polygonum multiflorum L. with colchicine treatment, and to clarify its effective induction and time for the increase of rhizome hypertrophy and effective components. Methods and Results : The induction of tetraploid P. multiflorum were done with colchicine treatment of 0.1, 0.5, and 1.0% conc. and the time was treated with 12, 24, and 48 hrs, respectively. DMSO 1% is treated for the effective penetration of colchicine with constant-temperature oven at 24C. Leaf explant was added the HR-A solution stained with HR-B solution, and then determined whether DNA contents of the doubles or not. Seed stand rate showed lower tendency as higher treatment concentration and as delayed the treatment time. When treated in DMSO 1% with 48 hr, seed stand was so small rate with 2%, and treated in 0.5% with 12 hr, seed stand was recorded higher rate with 33%. When treated in 0.5% colchicine with 24 hr, chimera and tetraploid induction rates were highest and obtained 29 individual chimera and 5 individual tetraploid. On overground growing, mean stem diameter of tetraploid (11.3 ㎜) is two times thicker than that of diploid (6.4 ㎜), with the vigorous growth. leaf length and width of tetraploid was 9.1 and 6.2 ㎝, respectively, and that of diploid was 6.8, and 5.1 ㎝, respectively. Stem diameter of a scape was also more thicker tendency in tetraploid than in diploid, chlorophyll contents over 29% existed in tetraploid. Investigated result from stoma no. and size of leaf with 400 magnification, stoma no. of diploid (26 individual) was four times higher than that of tetraploid (7 individuals), the size of tetraploid (38 ㎛) is twice longer in size of diploid (18 ㎛) and stoma area are broaden in four times. Seed length of tetraploid (3 ㎜) is longer than that of diploid (22 ㎜); but, seed width of tetraploid (22 ㎜) is widen than that of diploid (14 ㎜) in seed size comparison. That is, seed length is bigger in 1.4 times and seed width is bigger in 1.6 times, and seed surface area is broaden in two times. Conclusion : From above results, overground growing of tetraploid P. multiflorum is fairly better than that of native P. multiflorum. In the future, this tetraploid variety will promote to register when recognize the excellence than native species after tested the disease tolerance, underground growing characteristics, and key index component.

Background : The effect of ridge-up bed cultivation and stem training method on the plant growth and yield of Cynanachum willfordii was investigated. Methods and Results : Domestic variety of Cynanachum willfordii was tested at Chungbuk ARES under the following conditions. Nonsupporting (custom) and I-form supporting was used for stem training method, and ridge height was set as 30 (custom) and 60 cm (high ridge). Planting spacing was adjusted to 30 (interrow spacing) × 20 cm (intrarow spacing), and other major management was followed the method of standard cultivation for medicinal crop. Investigated result from leaf characteristics, leaf length was longer in high ridge cultivation (HRC) as 11.1 cm than custom cultivation (CC) as 10.6 cm. Leaf width is proved to be 12.8 (HRC) > 11.2 cm (CC). Leaf number is proved to be 294 (HRC) > 254/plant (CC), with higher number of 44/plant at HRC and weight/10 leaves were more heavy at HRC (4.9 g) than that of CC (2.6 g). It was more fruit setting at HRC over 15/plant. According to the stem training method and ridge height from nonsupporting cultivation, main root was 4.0 (CC), higher than that of HRC over 0.5/plant. However, root length was more longer in HRC (28.6) than that of CC (25.0 cm). Main root diameter was also showed more thicker pattern in HRC. From staking cultivation, root number of HRC was 7/plant, it was recorded more 3/plant than that of CC, and it was also same pattern in main root length and root diameter. By the standard of commercial root, yield of living roots in nonsupporting cultivation were 59.0g/plant (HRC), it was more heavy over 10.4g/plant than that of CC. In staking cultivation, HRC were recorded as 74.2g/plant, more heavy 6.9g/plant than that of CC. Yield from I-form support stem training and ridge-up bed cultivation of HRC was higher approxmiately 52.7% than that of CC. Conclusion : Overall growth by high ridge cultivation of I-form support was good and yield of HRC was also increased over 52.7% than that of CC.

충북농업기술원 마늘연구소에서는 2000년부터 수집한 유전자원 중 한지형인 ‘단양종’ 보다 조숙다수성이며 불완전추대의 특성을 가진 계통을 5년 동안 지속 선발하여 ‘단산’ 품종을 육성하였다. ‘단산’ 마늘의 추대 특성은 불완전 추대형으로 ‘단양종’의 완전 추대형과 뚜렷한 구별성을 갖는다. 따라서 화경이 출현하지 않아 수확 전에 쫑을 뽑을 필요가 없으며 숙기도 6일 정도 빨라 수확기 때 장마를 피할 수 있는 장점을 갖고 있다. 2차 생장비율은 3.3%로 ‘단양종’이 17.3% 보다 매우 낮아 이차생장율이 적은 상품성 마늘을 생산할 수 있다. ‘단산’의 통의 크기는 ‘단양종’ 보다 크고, 쪽 껍질의 색은 갈색을 띠며 안토시아닌 줄무늬가 존재한다. 수량은 1,131 kg/10 a로 ‘단양종’ 보다 18% 정도 높다.